{"id":28169,"date":"2021-07-15T14:29:20","date_gmt":"2021-07-15T06:29:20","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28169"},"modified":"2023-08-17T11:57:49","modified_gmt":"2023-08-17T03:57:49","slug":"caspase-1-%e8%9e%a2%e5%85%89%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk110-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=28169","title":{"rendered":"Caspase-1 \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fK110-100 (ABCAM ab39412)"},"content":{"rendered":"

Caspase-1 \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u00a0\u8ca8\u865f<\/strong>K110-100 (ABCAM ab39412)<\/span><\/span><\/span><\/a><\/span><\/p>\n

Caspase-1 Fluorometric Assay Kit<\/em><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39: ICE \u5bb6\u65cf\u86cb\u767d\u9176\/\u534a\u80f1\u5929\u51ac\u9176\u7684\u6fc0\u6d3b\u555f\u52d5\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u7d30\u80de\u51cb\u4ea1(apoptosis)\u6216\u5176\u4ed6\u7d30\u80de\u904e\u7a0b(cellular processes)\u3002 Caspase-1\/ICE \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u65b9\u4fbf\u7684\u65b9\u6cd5\u4f86\u6aa2\u6e2c\u8b58\u5225 YVAD \u5e8f\u5217\u7684 caspase \u7684\u6d3b\u6027\u3002\u8a72\u6e2c\u5b9a\u57fa\u65bc\u6aa2\u6e2c\u5e95\u7269 YVAD-AFC\uff08AFC\uff1a7-\u6c28\u57fa-4-\u4e09\u6c1f\u7532\u57fa\u9999\u8c46\u7d20\uff09\u7684\u88c2\u89e3\u3002 YVAD-AFC \u767c\u51fa\u85cd\u5149\uff08400 nm\uff09\uff1b\u901a\u904e caspase-1 \u6216\u76f8\u95dc caspase \u5207\u5272\u5e95\u7269\u5f8c\uff0c\u6e38\u96e2 AFC \u767c\u51fa\u9ec3\u7da0\u8272\u87a2\u5149 (505 nm)\uff0c\u53ef\u4f7f\u7528\u87a2\u5149\u8a08\u6216\u87a2\u5149\u5fae\u91cf\u6ef4\u5b9a\u677f\u8b80\u6578\u5668\u5c0d\u5176\u9032\u884c\u5b9a\u91cf\u3002\u5c07\u8655\u7406\u904e\u7684\u6a23\u54c1\u4e2d AFC \u7684\u87a2\u5149\u8207\u672a\u7d93\u8655\u7406\u7684\u5c0d\u7167\u9032\u884c\u6bd4\u8f03\uff0c\u53ef\u4ee5\u78ba\u5b9a caspase-1 \u6d3b\u6027\u7684\u589e\u52a0\u500d\u6578\u3002<\/p>\n

<\/p>\n

\u529f\u80fd\u6aa2\u6e2c\uff1aCaspase 1 \u6aa2\u6e2c\u8a66\u5291\u76d2 (ab39412)<\/strong><\/span><\/div>\n
\n

Badding MA \u7b49\u4eba\u7814\u7a76\u4e86\u6c27\u5316\u92a6\u932b (ITO) \u7684\u7d30\u80de\u6bd2\u6027\u3002\u6b64\u524d\uff0cITO \u5728\u57f9\u990a\u7d30\u80de\u4e2d\u986f\u793a\u51fa\u7d30\u80de\u6bd2\u6027\uff0c\u5728\u80ba\u90e8\u52d5\u7269\u6a21\u578b\u4e2d\u986f\u793a\u51fa\u4fc3\u708e\u4f5c\u7528\u3002ITO \u7528\u65bc\u88fd\u9020\u89f8\u6478\u5c4f\u548c\u6db2\u6676\u986f\u793a\u96fb\u5b50\u7522\u54c1\u7684\u900f\u660e\u5c0e\u96fb\u5857\u5c64\u3002RAW \u7d30\u80de\u4ee5 5 x 10 5 \u500b<\/sup>\u00a0\u7d30\u80de\/\u5b54\u92ea\u677f\uff0c\u4e26\u7528 SITO\u3001LPS \u6216 Min-U-sil \u8655\u7406\uff08\u7d30\u80de\u9996\u5148\u7528 LPS \u5f15\u767c\uff09\u3002\u6d17\u6ecc\u3001\u88c2\u89e3\u7d30\u80de\uff0c\u4e26\u4f7f\u7528 Caspase 1 \u6e2c\u5b9a\u8a66\u5291\u76d2 (ab39412) \u6e2c\u5b9a 100 \u03bcg \u88c2\u89e3\u7269\u3002\u4f7f\u7528 PBS \u4f5c\u70ba\u5c0d\u7167\u3002\u6240\u6709\u689d\u4ef6\u5747\u5728\u96d9\u5b54\u4e2d\u904b\u884c\uff0c\u4e26\u4e14\u6bcf\u500b\u6642\u9593\u9ede\u9032\u884c\u4e09\u500b\u7368\u7acb\u5be6\u9a57\u3002<\/p>\n

<\/p>\n

\u529f\u80fd\u6aa2\u6e2c\uff1aCaspase 1 \u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09(ab39412)<\/strong><\/span><\/div>\n
\n

Caspase 1 (\u00a0<\/span>ab39901<\/span><\/a>\u00a0) \u7684\u6ef4\u5b9a\uff08\u6e1b\u53bb\u80cc\u666f\u4fe1\u865f\uff0c\u91cd\u8907\uff1b+\/- SD\uff09\u3002<\/span><\/p>\n<\/div>\n<\/div>\n\n\n\n\n\n\n\n\n\n\n
Detection Method<\/strong><\/td>\nFluorescence (400 nm excitation filter and 505 nm emission filter)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetect early\/middle stages of apoptosis; differentiate apoptosis from necrosis.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple one-step procedure; takes 1-2 hours
\n\u2022 Fast and convenient
\n\u2022 Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Cell Lysis Buffer
\n\u2022 2X Reaction Buffer
\n\u2022 YVAD-AFC (1mM)
\n\u2022 DTT (1 M)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

 <\/p>\n

Min Guo, et al., (2018) Ketogenic Diet Improves Brain Ischemic Tolerance and Inhibits NLRP3 Inflammasome Activation by Preventing Drp1-Mediated Mitochondrial Fission and Endoplasmic Reticulum Stress,\u00a0Frontiers in Molecular Neuroscience, Mar. 2018, 29662437<\/a><\/p>\n

Ahn H et al., (2017) Isorhamnetin and hyperoside derived from water dropwort inhibits inflammasome activation,\u00a0Phytomedicine, 2017, 24:77-86<\/a><\/p>\n

Lim et al., Regiospecific Methylation of a Dietary Flavonoid Scaffold Selectively Enhances IL-1\u03b2 Production following Toll-like Receptor 2 Stimulation in THP-1 Monocytes.\u00a0J. Biol. Chem., Jul 2013; 288: 21126 – 21135.<\/a><\/p>\n

Koenen, T. B. et. al. The Inflammasome and Caspase-1 Activation: A New Mechanism Underlying Increased Inflammatory Activity in Human Visceral Adipose Tissue.\u00a0Endocrinology, Oct 2011; 152: 3769 – 3778.<\/a><\/p>\n

Cheng, S. C. et. al. The dectin-1\/inflammasome pathway is responsible for the induction of protective T-helper 17 responses that discriminate between yeasts and hyphae of Candida albicans.\u00a0J. Leukoc. Biol., Aug 2011; 90: 357 – 366.<\/a><\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Caspase-1 \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u00a0\u8ca8\u865fK110-100 (ABCAM ab39412) Caspase […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261,3],"tags":[1098],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28169"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28169"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28169\/revisions"}],"predecessor-version":[{"id":45133,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28169\/revisions\/45133"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28169"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28169"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28169"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}