{"id":28150,"date":"2021-07-07T13:23:10","date_gmt":"2021-07-07T05:23:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28150"},"modified":"2022-07-25T09:54:29","modified_gmt":"2022-07-25T01:54:29","slug":"human-cd274pd-l1-protein-his-tag-animal-free%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9fprp2021","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=28150","title":{"rendered":"Human CD274\/PD-L1 protein, His tag (Animal-Free)\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fPRP2021"},"content":{"rendered":"

Human CD274\/PD-L1 protein, His tag (Animal-Free)\u91cd\u7d44<\/strong>\u86cb\u767d<\/strong> |<\/strong>\u8ca8\u865f<\/strong>PRP2021<\/a><\/span><\/p>\n

\"abbkine02\"<\/p>\n

\u7a0b\u5e8f\u6027\u6b7b\u4ea1-1\u914d\u9ad4-1\uff08Programmed death-1 ligand-1, PD-L1\u3001CD274\u3001B7-H1\uff09\u5df2\u88ab\u9451\u5b9a\u70ba\u514d\u75ab\u6291\u5236\u53d7\u9ad4\u7a0b\u5e8f\u6027\u6b7b\u4ea1-1\uff08immunoinhibitory receptor programmed death-1, PD1\/PDCD1\uff09\u7684\u914d\u9ad4\uff0c\u4e26\u5df2\u88ab\u8b49\u660e\u5728\u514d\u75ab\u8abf\u7bc0(immune responses)\u4e2d\u767c\u63ee\u4f5c\u7528\u3002\u53cd\u61c9\u548c\u5916\u5468\u8010\u53d7\u3002\u5728\u8a98\u5c0e(induction)\u548c\u7dad\u6301(maintenance)\u5c0d\u81ea\u8eab\u7684\u514d\u75ab\u8010\u53d7\u4e2d\u8d77\u95dc\u9375\u4f5c\u7528\u3002\u4f5c\u70ba\u6291\u5236\u6027\u53d7\u9ad4 PDCD1\/PD-1 \u7684\u914d\u9ad4(ligand)\uff0c\u8abf\u7bc0 T \u7d30\u80de\u7684\u6fc0\u6d3b\u95be\u503c(activation threshold)\u4e26\u9650\u5236 T \u7d30\u80de\u6548\u61c9\u53cd\u61c9(T-cell effector response)\u3002\u901a\u904e\u672a\u77e5\u7684\u6fc0\u6d3b\u53d7\u9ad4\uff0c\u53ef\u5171\u523a\u6fc0\u4e3b\u8981\u7522\u751f\u767d\u7d30\u80de\u4ecb\u7d20 10 (IL10) \u7684 T \u7d30\u80de\u4e9e\u7fa4(T-cell subsets)\u3002 PDCD1 \u4ecb\u5c0e\u7684\u6291\u5236\u901a\u8def\u88ab\u816b\u7624\u5229\u7528\u4f86\u6e1b\u5f31\u6297\u816b\u7624\u514d\u75ab\u4e26\u9003\u907f\u514d\u75ab\u7cfb\u7d71\u7684\u7834\u58de\uff0c\u5f9e\u800c\u4fc3\u9032\u816b\u7624\u5b58\u6d3b\u3002\u8207 PDCD1\/PD-1 \u7684\u76f8\u4e92\u4f5c\u7528\u6291\u5236\u7d30\u80de\u6bd2\u6027 T \u6dcb\u5df4\u7d30\u80de (CTL) \u6548\u61c9\u5b50\u529f\u80fd\uff08\u76f8\u4f3c\u6027\uff09\u3002 PDCD1 \u4ecb\u5c0e\u901a\u8def\u7684\u963b\u65b7\u5c0e\u81f4\u8017\u7aed T \u7d30\u80de\u8868\u578b\u7684\u9006\u8f49\u548c\u6297\u816b\u7624\u53cd\u61c9(anti-tumor response)\u7684\u6b63\u5e38\u5316\uff0c\u70ba\u764c\u75c7\u514d\u75ab\u6cbb\u7642\u63d0\u4f9b\u4e86\u57fa\u672c\u539f\u7406\u3002<\/p>\n

\u64cd\u4f5c\u624b\u518a\u4e0b\u8f09<\/a><\/strong><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: <\/strong>Human CD274\/PD-L1 protein, His tag (Animal-Free)\u91cd\u7d44<\/strong>\u86cb\u767d<\/strong> \uff0c<\/strong> \u7531HEK293 Cells\u751f\u7522\uff0c\u80fa\u57fa\u9178\u5e8f\u5217\u6e90\u81ea\u4eba CD274 (NP_054862.1) (Met1-Thr239) \u7684\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u5728 C \u7aef\u5e36\u6709 6 His \u6a19\u7c64\u3002\u91cd\u7d44\u4eba CD274\u86cb\u767d\uff0c \u7531 227 \u500b\u6c28\u57fa\u9178\u7d44\u6210\uff0c\u9810\u8a08\u5206\u5b50\u91cf\u70ba 26.8 KD\u3002\u7531\u65bc\u7cd6\u57fa\u5316(glycosylation)\uff0c\u5b83\u5728\u9084\u539f\u689d\u4ef6\u4e0b\u5728 SDS-PAGE \u4e2d\u9077\u79fb\u70ba\u5927\u7d04 33-40 KD\u3002\u7d14\u5ea6>98% (determined by SDS-PAGE); \u5167\u6bd2\u7d20(Endotoxin): < 1.0 EU per \u00b5g of the protein ( determined by the LAL method)\u3002Alternative<\/strong> Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1<\/p>\n

\"Fig.<\/p>\n

Fig. SDS-PAGE analysis of Human CD274\/PD-L1 protein, His tag.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nHuman CD274\/PD-L1 protein, His tag (Animal-Free)<\/a><\/td>\n<\/tr>\n
Sequence<\/strong><\/td>\nAmino acid sequence derived from human CD274 (NP_054862.1) (Met1-Thr239)was expressed with a 6 His tag at the C-terminus.<\/td>\n<\/tr>\n
Protein length<\/strong><\/td>\nThe recombinant human CD274 consists of 227 amino acids and predicts a molecular mass of 26.8 KD. It migrates as an approximately 33-40 KD band in SDS-PAGE under reducing conditions due to glycosylation.<\/td>\n<\/tr>\n
Preparation method<\/strong><\/td>\nHEK293 Cells<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n>98% as determined by SDS-PAGE<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nProgrammed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLyophilized from sterile PBS, pH 7.4.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\nEndotoxin: < 1.0 EU per \u00b5g of the protein as determined by the LAL method.<\/td>\n<\/tr>\n
Molecular weight<\/strong><\/td>\n26.8 KD<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\nAlways centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD274\/PD-L1 protein using the buffer we provided not less than 100 \u00b5g\/ml, which can then be further diluted to other aqueous solutions.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nLyophilized Human CD274 protein product should be stored desiccated below -20\u00b0C. Upon reconstitution, the protein should be stored at 4\u00b0C between 2-7 days and for future use below -20\u00b0C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nThe product is shipped at ambient temperature.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n29126<\/a><\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nProgrammed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1<\/td>\n<\/tr>\n
Accession<\/strong><\/td>\nQ9NZQ7<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Human CD274\/PD-L1 protein, His tag (Animal-Free)\u91cd\u7d44\u86cb\u767d |\u8ca8 […]<\/p>\n","protected":false},"author":3,"featured_media":25491,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1103,1102],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28150"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28150"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28150\/revisions"}],"predecessor-version":[{"id":33478,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28150\/revisions\/33478"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/25491"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28150"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28150"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28150"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}