{"id":27948,"date":"2021-06-28T14:18:48","date_gmt":"2021-06-28T06:18:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27948"},"modified":"2022-07-19T14:11:44","modified_gmt":"2022-07-19T06:11:44","slug":"recombinant-nerve-growth-factor-ngf%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bdprokaryotic-expression-%e8%b2%a8%e8%99%9frpa105ra01","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27948","title":{"rendered":"Recombinant Nerve Growth Factor (NGF)\u91cd\u7d44\u86cb\u767dProkaryotic expression | \u8ca8\u865fRPA105Ra01"},"content":{"rendered":"

\u9644<\/em><\/strong>Protein marker <\/em><\/strong>\u6216<\/em><\/strong> positive control <\/em><\/strong><\/p>\n

Recombinant Nerve Growth Factor (NGF)<\/strong>\u91cd\u7d44\u86cb\u767dProkaryotic expression <\/strong>| <\/strong>\u8ca8\u865f<\/strong>RPA105Ra01<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Cloud clone\u63d0\u4f9b\u539f\u6838\u53ca\u771f\u6838\u751f\u7269\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\u7cfb\u5217\u5546\u54c1\uff1b\u5176\u4e2dRecombinant Nerve Growth Factor (NGF)\u91cd\u7d44\u86cb\u767d (Rattus norvegicus (Rat))\uff0c\u70ba\u539f\u6838\u751f\u7269 (E.coli) Prokaryotic expression\u8868\u73fe\u4e4b\u91cd\u7d44\u86cb\u767d\uff1b\u86cb\u767d\u8cea\u8403\u53d6\u5f8c\u5167\u6bd2\u7d20\u6aa2\u6e2c-LAL method <1.0EU per 1\u00b5g\u3002\u7d14\u5ea6> 90%\u3002\u8868\u73fe\u4f4d\u7f6eLys23~Ala140\uff1bN-terminal\u5177 His Tag\u4fee\u98fe\u3002\u6b64\u86cb\u767d\u8cea\u65bc\u7d30\u80de\u5167\u8868\u73fe\u4f4d\u7f6e\u70baMembrane\u3001Nucleus\u3001Cytoplasm\uff1b\u9810\u6e2c\u5206\u5b50\u91cf17.8kDa\u3002\u53ef\u61c9\u7528\u65bcPositive Control; Immunogen; SDS-PAGE; WB\u7b49\u4e0d\u540c\u7814\u7a76\u3002<\/p>\n

\u86cb\u767d\u8cea\u5e8f\u5217<\/p>\n

\u86cb\u767d\u8cea\u4fdd\u5b58\u578b\u5f0f<\/strong><\/p>\n

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. \u70ba\u51b7\u51cd\u4e7e\u71e5\u5305\u88dd\u3002\u4f7f\u7528\u524dReconstitute in PBS (pH7.4) \u4f7f\u6700\u5f8c\u6fc3\u5ea6\u70ba 0.1-1.0 mg\/mL\u907f\u514dvortex. \u77ed\u671f\u4fdd\u5b58Store at 2-8\u00b0C for one month.\u5206\u88dd-80\u00b0C\u9577\u671f12 \u500b\u6708\u3002\u8acb\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002<\/p>\n

Recombinant Nerve Growth Factor (NGF)<\/strong>\u91cd\u7d44\u86cb\u767d<\/strong>\u76f8\u95dc\u4e4b\u6297\u9ad4<\/strong> | <\/strong>\u86cb\u767d\u8cea<\/strong> \u7522\u54c1<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/td>\nRelated products for research use of Rattus norvegicus (Rat) Organism species<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
RPA105Ra01<\/td>\nRecombinant Nerve Growth Factor (NGF)<\/strong><\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
APA105Ra01<\/td>\nActive Nerve Growth Factor (NGF)<\/strong><\/a><\/td>\nCell\u00a0culture;\u00a0Activity\u00a0Assays.<\/td>\n<\/tr>\n
EPA105Ra61<\/td>\nEukaryotic Nerve Growth Factor (NGF)<\/strong><\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
PAA105Ra01<\/td>\nPolyclonal Antibody to Nerve Growth Factor (NGF)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
MAA105Ra21<\/td>\nMonoclonal Antibody to Nerve Growth Factor (NGF)<\/strong><\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
SEA105Ra<\/td>\nELISA Kit for Nerve Growth Factor (NGF)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
WEA105Ra<\/td>\nWide-range ELISA Kit for Nerve Growth Factor (NGF)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
SCA105Ra<\/td>\nCLIA Kit for Nerve Growth Factor (NGF)<\/strong><\/a><\/td>\nChemiluminescent immunoassay for Antigen Detection.<\/td>\n<\/tr>\n
KSA105Ra01<\/td>\nELISA Kit DIY Materials for Nerve Growth Factor (NGF)<\/strong><\/a><\/td>\nMain materials for “Do It (ELISA Kit) Yourself”.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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\u00a0<\/strong><\/p>\n

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\u9644Protein marker \u6216 positive control Recombinant Nerve Gr […]<\/p>\n","protected":false},"author":3,"featured_media":16383,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1117],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27948"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27948"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27948\/revisions"}],"predecessor-version":[{"id":27951,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27948\/revisions\/27951"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/16383"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27948"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27948"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27948"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}