{"id":27927,"date":"2021-06-25T16:44:06","date_gmt":"2021-06-25T08:44:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27927"},"modified":"2022-07-19T14:11:55","modified_gmt":"2022-07-19T06:11:55","slug":"%e7%b4%b0%e8%83%9e%e7%b5%84%e7%b9%94mirna%e8%90%83%e5%8f%96%e5%a5%97%e7%b5%84-%e8%b2%a8%e8%99%9fk1456-50","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27927","title":{"rendered":"\u7d30\u80de\/\u7d44\u7e54miRNA\u8403\u53d6\u5957\u7d44 | \u8ca8\u865fK1456-50"},"content":{"rendered":"

\u7d30\u80de\/\u7d44\u7e54miRNA\u8403\u53d6\u5957\u7d44 | \u00a0\u8ca8\u865f<\/strong>K1456-50<\/a><\/span><\/p>\n

miRNA Extraction Kit<\/p>\n

\u7522\u54c1\u4ecb\u7d39:. BioVision \u7684 miRNA Extraction Kit \u7528\u65bc\u5f9e\u7d30\u80de\u548c\u7d44\u7e54\u4e2d\u7d14\u5316 miRNA\u3002\u8a72\u8a66\u5291\u76d2\u57fa\u65bc\u6539\u9032\u7684\u786b\u6c30\u9178\u80cd-\u82ef\u915a(guanidine thiocyanate- phenol)\u4e00\u6b65\u6cd5\u548c\u4f7f\u7528\u96e2\u5fc3\u67f1\u6d17\u812b miRNA\u3002\u5728\u8a72\u8a66\u5291\u76d2\u4e2d\uff0c\u6838\u7cd6\u6838\u9178\u9176(ribonucleases)\u88ab\u6ec5\u6d3b\uff0c\u57fa\u56e0\u7d44 DNA\u300118S \u548c 28S RNA \u4f7f\u7528\u96e2\u5fc3\u67f1(spin columns)\u53bb\u9664\u3002\u901a\u904e\u4e00\u7cfb\u5217\u6d17\u812b-\u96e2\u5fc3\u6b65\u9a5f\u53bb\u9664\u7d30\u80de\u4ee3\u8b1d\u7269\u548c\u86cb\u767d\u8cea\u3002\u96a8\u5f8c\uff0c\u5c07 microRNA\uff08\u5305\u62ec miRNA\u3001snRNA \u548c\u5176\u4ed6\u5c0f\u65bc 200 bp \u7684 RNA\uff09\u5f9e\u4e0d\u542b RNase \u7684\u6c34\u4e2d\u5f9e\u7b2c\u4e8c\u500b\u96e2\u5fc3\u67f1\u4e2d\u6d17\u812b\u3002\u7d14\u5316\u7684 miRNA \u53ef\u7528\u65bc\u4e0b\u6e38\u61c9\u7528\uff0c\u5305\u62ec RNA \u6e2c\u5e8f(RNA sequencing)\u3001\u5fae\u9663\u5217(microarray)\u3001RT-PCR\u3001Northern Blot \u7b49\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK1456-50<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n50 preps<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nmiRNA can be purified from cells and tissues using spin columns.<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nRNA sequencing, microarray, RT-PCR, Northern Blot<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Highly pure miRNA for downstream applications
\n\u2022 High quality Spin Columns with silica membranes
\n\u2022 Genomic DNA is efficiently removed by buffer MRL
\n\u2022 Purify miRNA, snRNA,and other RNA less than 200 bp<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Buffer MRL
\n\u2022 *Buffer RW
\n\u2022 RNase-free water
\n\u2022 **RNase-free water (for 70% Ethanol)
\n\u2022 RNase-free Spin Column RA
\n\u2022 RNase-free Spin Column RB
\n\u2022 Collection Tube<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\nMultiple Temperatures<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nRT<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\/\u7d44\u7e54miRNA\u8403\u53d6\u5957\u7d44 | \u00a0\u8ca8\u865fK1456-50 miRNA Extraction Kit \u7522\u54c1\u4ecb\u7d39: […]<\/p>\n","protected":false},"author":3,"featured_media":25717,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1118],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27927"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27927"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27927\/revisions"}],"predecessor-version":[{"id":27936,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27927\/revisions\/27936"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/25717"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27927"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27927"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27927"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}