{"id":27827,"date":"2021-06-16T15:11:05","date_gmt":"2021-06-16T07:11:05","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27827"},"modified":"2022-07-19T14:13:00","modified_gmt":"2022-07-19T06:13:00","slug":"durvalumab-elisa-%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fe4925-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27827","title":{"rendered":"Durvalumab ELISA \u8a66\u5291\u76d2 | \u8ca8\u865fE4925-100"},"content":{"rendered":"

Durvalumab ELISA \u8a66\u5291\u76d2 | \u8ca8\u865f<\/strong>E4925-100<\/a><\/span><\/p>\n

BioSim\u2122<\/em><\/strong> Durvalumab (Human) ELISA Kit<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39: Durvalumab \u662f\u4e00\u7a2e\u4eba IgG1 \u55ae\u514b\u9686\u6297\u9ad4(human IgG1 monoclonal antibody)\uff0c\u53ef\u7d50\u5408\u4f75\u6291\u5236\u7a0b\u5e8f\u6027\u7d30\u80de\u6b7b\u4ea1\u914d\u9ad4 1 (inhibits programmed cell death ligand 1, PD-L1)\u3002 PD-L1 \u662f\u4e00\u7a2e\u91cd\u8981\u7684\u514d\u75ab\u6aa2\u67e5\u9ede\u5206\u5b50(immune checkpoint)\uff0c\u8868\u9054\u65bc\u816b\u7624\u7d30\u80de(tumor cells)\u548c\u816b\u7624\u6d78\u6f64\u514d\u75ab\u7d30\u80de(tumor-infiltrating immune cells)\u4e0a\u3002 PD-L1 \u8207\u5176\u4f4d\u65bc\u6d3b\u5316 T \u7d30\u80de\u548c\u6297\u539f\u5448\u905e\u7d30\u80de\u8868\u9762\u7684\u53d7\u9ad4 PD-1 \u548c B7.1 \u7d50\u5408\uff0c\u9032\u800c\u6291\u5236\u7d30\u80de\u6bd2\u6027 T \u7d30\u80de\u6d3b\u6027(cytotoxic T-cell activity)\u3001T \u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u56e0\u5b50(cytokines)\u7684\u7522\u751f\u3002\u55ae\u514b\u9686\u6297\u9ad4(monoclonal antibody)\u8207 PD-L1 \u7d50\u5408\u4e26\u6291\u5236\u5176\u6297\u816b\u7624\u514d\u75ab\u53cd\u61c9\uff0c\u5f9e\u800c\u5728\u4e0d\u5f15\u8d77\u6297\u9ad4\u4f9d\u8cf4\u6027\u7d30\u80de\u6bd2\u6027(antibody-dependent cellular cytotoxicity)\u7684\u60c5\u6cc1\u4e0b\u6e1b\u5c11\u816b\u7624\u751f\u9577\u3002\u539f\u59cb\u6297\u9ad4\u88ab\u6279\u51c6\u7528\u65bc\u6cbb\u7642\u5c40\u90e8\u665a\u671f\u6216\u8f49\u79fb\u6027\u5c3f\u8def\u4e0a\u76ae\u764c\u548c\u5c40\u90e8\u665a\u671f\u4e0d\u53ef\u5207\u9664\u7684\u975e\u5c0f\u7d30\u80de\u80ba\u764c (non-small cell lung cancer , NSCLC)\u3002 BioSim\u2122 Durvalumab ELISA \u8a66\u5291\u76d2\u4ee5\u9ad8\u9748\u654f\u5ea6\u548c\u53ef\u91cd\u8907\u6027\u6e2c\u91cf\u4eba\u8840\u6e05\u6216\u8840\u6f3f\u4e2d\u7684\u6e38\u96e2 Durvalumab \u6fc3\u5ea6\u3002\u8a72\u8a66\u5291\u76d2\u57fa\u65bc\u593e\u5fc3ELISA\u539f\u7406\u3002\u5c07\u6a19\u6e96\u54c1\u548csamples\uff08\u8840\u6e05\u6216\u8840\u6f3f\uff09\u52a0\u5165\u5857\u6709 Durvalumab \u53cd\u61c9\u7269\u7684\u5fae\u91cf\u6ef4\u5b9a\u677f\u3002\u6eab\u80b2\u5f8c\uff0c\u6d17\u6ecc\u5b54\u3002\u6dfb\u52a0 HRP \u5076\u806f\u63a2\u91dd\u4e26\u8207\u7531\u5b54\u8868\u9762\u53cd\u61c9\u7269\u6355\u7372\u7684 Durvalumab \u7d50\u5408\u3002\u5b75\u80b2\u5f8c\uff0c\u6d17\u6ecc\u5b54\u4e26\u901a\u904e\u6dfb\u52a0 TMB \u8272\u539f\u5e95\u7269\u6aa2\u6e2c\u9176\u6d3b\u6027\u3002\u6700\u5f8c\uff0c\u7528\u9178\u6027\u7d42\u6b62\u6eb6\u6db2\u7d42\u6b62\u53cd\u61c9\u3002\u986f\u8272\u8207\u6a23\u54c1\u6216\u6a19\u6e96\u54c1\u4e2d Durvalumab \u7684\u91cf\u6210\u6b63\u6bd4\u3002\u6a23\u54c1\u7d50\u679c\u53ef\u4ee5\u76f4\u63a5\u4f7f\u7528\u6a19\u6e96\u66f2\u7dda\u78ba\u5b9a\u3002<\/p>\n

\u8a66\u5291\u5fc5\u9808\u914d\u5408ELISA\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100<\/a><\/span> (\u57fa\u672c\u6b3e405\u3001450\u3001492\u3001630 nm \/\u9032\u968e\u6b3e\u53ef\u64f4\u589e\u81f37\u500b\u6ffe\u7247) \u4f7f\u7528\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nE4925-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n96 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe Sandwich ELISA kit is used for in vitro Quantitative determination of free Durvalumab in human serum and plasma samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Detection Range: 10 – 300 ng\/ml
\n\u2022 Recovery rate: < 100 \u00b1 30% with known concentrations of normal human serum samples
\n\u2022 For in vitro Quantitative determination of free Durvalumab in human serum and plasma samples
\n\u2022 Cross Reactivity: Except for Durvalumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins
\n\u2022 Sensitivity: 10 ng\/mL
\n\u2022 Assay Precision: Intra-Assay and Inter-Assay CV < 30%<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Microtiter Plate
\n\u2022 Durvalumab Standards (S1 \u2013 S7)
\n\u2022 Assay Buffer
\n\u2022 HRP-conjugate Probe
\n\u2022 TMB substrate (Avoid light)
\n\u2022 Stop Solution
\n\u2022 Wash buffer (20X)
\n\u2022 Plate sealers<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u8a66\u5291\u5fc5\u9808\u914d\u5408ELISA\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100<\/a> (\u57fa\u672c\u6b3e405\u3001450\u3001492\u3001630 nm \/\u9032\u968e\u6b3e\u53ef\u64f4\u589e\u81f37\u500b\u6ffe\u7247) \u4f7f\u7528\u3002Durvalumab ELISA \u8a66\u5291\u76d2(\u8ca8\u865fE4558-100) \u6e2c\u91cf\u65b9\u6cd5\u70ba\u5438\u6536\u5149450nm\u3002<\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong>\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Durvalumab ELISA \u8a66\u5291\u76d2 | \u8ca8\u865fE4925-100 BioSim\u2122 Durvalumab ( […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1124],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27827"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27827"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27827\/revisions"}],"predecessor-version":[{"id":27829,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27827\/revisions\/27829"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27827"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27827"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27827"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}