{"id":27796,"date":"2021-06-07T14:39:33","date_gmt":"2021-06-07T06:39:33","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27796"},"modified":"2023-08-01T12:52:37","modified_gmt":"2023-08-01T04:52:37","slug":"chekine-hydrogen-peroxide-h2o2-assay-kit-%e8%b2%a8%e8%99%9fktb1041","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27796","title":{"rendered":"H2O2\u904e\u6c27\u5316\u6c2b\u6aa2\u6e2c | CheKine\u2122 Hydrogen Peroxide (H2O2) Assay Kit \u8ca8\u865fKTB1041"},"content":{"rendered":"

CheKine\u2122 Hydrogen Peroxide (H2O2) Assay Kit | <\/strong>\u8ca8\u865f<\/strong>KTB1041\u00a0 96\u500b\u5206\u6790\u00a0 \u00a0480\u500b\u5206\u6790<\/span><\/a><\/span><\/p>\n

Detect biological samples at a level of 1-20 \u00b5M.<\/p>\n

\"Fig.<\/p>\n

\u904e\u6c27\u5316\u6c2b (Hydrogen Peroxide , H2O2)\uff0c\u4e00\u7a2e\u6d3b\u6027\u6c27\u4ee3\u8b1d(reactive oxygen metabolic) \u526f\u7522\u7269\uff0c\u65e2\u662f\u7d30\u80de\u5167\u4fe1\u865f(intracellular signaling messenger)\u7684\u4fe1\u4f7f\uff0c\u4e5f\u662f\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u7684\u4f86\u6e90\u3002\u904e\u6c27\u5316\u6c2b(Hydrogen peroxide)\u901a\u904e\u591a\u7a2e\u6a5f\u5236\u5728\u7d30\u80de\u4e2d\u7522\u751f\uff0c\u4f8b\u5982\u4e2d\u6027\u7c92\u7d30\u80de(neutrophils)\u548c\u5de8\u566c\u7d30\u80de(macrophages)\u7522\u751f NOX \u4ecb\u5c0e\u7684 ROS\uff08NOX-mediated ROS production\uff09\uff0c\u6216\u7531\u7dda\u7c92\u9ad4\u547c\u5438(mitochondrial respiration)\u904e\u7a0b\u4e2delectron leak\u7522\u751f\u7684\u8d85\u6c27\u9670\u96e2\u5b50\u6b67\u5316\u9176(dismutase of superoxide anions)\u7522\u751f\u3002\u901a\u904e NF-\u03baB \u548c\u5176\u4ed6\u56e0\u7d20\u8d77\u4f5c\u7528\uff0c\u6c2b\u904e\u6c27\u5316\u7269\u4ecb\u5c0e\u7684\u901a\u8def\u8207\u54ee\u5598(asthma)\u3001\u708e\u6027\u95dc\u7bc0\u708e(inflammatory arthritis)\u3001\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis)\u3001\u7cd6\u5c3f\u75c5\u8840\u7ba1\u75c5\u8b8a(diabetic vasculopathy)\u3001\u9aa8\u8cea\u758f\u9b06\u75c7(osteoporosis)\u3001\u795e\u7d93\u9000\u884c\u6027\u75be\u75c5(neurodegenerative diseases)\u3001\u5510\u6c0f\u7d9c\u5408\u75c7(Down\u2019s syndrome)\u548c\u514d\u75ab\u7cfb\u7d71\u75be\u75c5(immune system diseases)\u6709\u95dc\u3002<\/p>\n

\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

CheKine\u2122 \u904e\u6c27\u5316\u6c2b\u6aa2\u6e2c\u8a66\u5291\u76d2(CheKine\u2122 Hydrogen Peroxide Assay Kit, KTB1041) \u00a0\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u6613\u7528\u7684\u6bd4\u8272\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u8840\u6e05(serum)\u3001\u8840\u6f3f(plasma)\u3001\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2(cell culture supernatants)\u3001\u7d44\u7e54(tissue)\/\u7d30\u80de\u88c2\u89e3\u6db2(cell lysates)\u548c\u5176\u4ed6\u751f\u7269\u6db2\u9ad4(other biological fluids)\u4e2d\u7684\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cH2O2 \u5c07\u4e9e\u9435\u6c27\u5316(H2O2 oxidize ferrous)\u70ba\u4e09\u50f9\u9435\u96e2\u5b50(ferric ion)\uff0c\u4e8c\u7532\u82ef\u915a\u6a59(xylenol orange)\u4ee5\u9ad8\u9078\u64c7\u6027\u7d50\u5408\u4e09\u50f9\u9435\u96e2\u5b50(ferric ion)\uff0c\u5f62\u6210\u6709\u8272\uff08\u7d2b\u8272\uff09\u7522\u7269\uff0c\u53ef\u901a\u904e\u6bd4\u8272\u6cd5\u5728 OD 580 nm \u8655\u9032\u884c\u6e2c\u91cf\u3002\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u904e\u6c27\u5316\u6c2b\u8207\u7372\u5f97\u7684\u4fe1\u865f\u6210\u6b63\u6bd4\u3002<\/p>\n

\"ELISA<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0(\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08KTB1041\u00a0 \u03bb= 580 nm\uff09<\/span><\/span><\/a><\/p>\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Hydrogen Peroxide (H2O2) Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nCheKine\u2122 Hydrogen Peroxide Assay Kit provides a simple and easy colorimetric assay for measuring hydrogen peroxide in serum, plasma, cell culture supernatants, tissue\/cell lysates and other biological fluids. In the assay, H2O2 oxidize ferrous to ferric ion, and xylenol orange binds ferric ion with high selectivity to form a colored (purple) product that can be measured by colorimetric method at OD 580 nm. The Hydrogen Peroxide present in the sample is proportional to the signal obtained.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Reaction Buffer
\n\u2022 H2O2 Standard (1M)
\n\u2022 Assay Buffer (10X)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of Hydrogen Peroxide in serum, plasma, cell\/tissue samples, urine and other biological fluids.
\n\u2022 Detect biological samples at a level of 1-20 \u00b5M.
\n\u2022 Simple procedures, fase and convenient, and more stable.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.
\n\u2022 Fresh samples are necessary for good results. If not assayed immediately, samples can be stored at -80\u00b0C for one month.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at -20\u00b0C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e8\u00a9\u00a6\u00e5\u008a\u0091\"\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n\n\n\n\n\n
Product name<\/strong><\/td>\nPurKine\u2122 His-Tag Cu-IDA Resin<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nPurKine\u2122 His-Tag Cu-IDA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90\u03bcm beads of highly cross-linked 4% agarose, to which Iminodiacetic Acid (IDA) has been coupled. The chelating group has then been charged with copper ions (Cu2+). Tests confirm that performance equals or exceeds popular Cu-IDA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nNi-NTA; His resin; Ni-NTA resin; His tag resin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Product Properties<\/h3>\n\n\n\n\n\n\n\n
Formulation<\/strong><\/td>\nLiquid solution<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 High capacity<\/strong>\u2014More than 50 mg of 6xHis-tagged protein per milliliter of resin.
\n\u2022 Versatile<\/strong>\u2014For purification under native or denaturing conditions.
\n\u2022 Cost-effective<\/strong>\u2014No performance decrease after at least five repeated uses of the same batch of resin.
\n\u2022 Flexible<\/strong>\u2014available in multiple formats including bulk resin, spin columns and complete kits.<\/td>\n<\/tr>\n
Storage buffer<\/strong><\/td>\nLiquid in PBS buffer containing 20% ethanol.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStable for one year at 2-8\u00b0C from date of shipment. Do not freeze.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nBlue ice<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e8\u00a9\u00a6\u00e5\u008a\u0091\"<\/p>\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u7522\u54c1\u540d\u7a31<\/td>\n\u80de\u5668\u67d3\u8272\u4f4d\u7f6e<\/td>\n\u87a2\u5149\u6ce2\u6bb5<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n\u7c92\u7dda\u9ad4(mitochondria)<\/td>\nEx \/ Em = 490\/523 nm<\/td>\n\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n\u5fae\u7ba1\u86cb\u767d(Tubulin)<\/td>\nEx\/Em = 500\/520 nm<\/td>\n\u6d3b\u7d30\u80de\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n\u7d30\u80de\u819c<\/a>\u00a0(plasma membrane)<\/td>\nEx\/Em = 484\/501 nm<\/td>\n\u6d3b\u7d30\u80de\u3001\u56fa\u5b9a\u61f8\u6d6e\u3001\u8cbc\u9644\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n\u7d30\u80de\u6838 dsDNA<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\nLysosome<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

CheKine\u2122 Hydrogen Peroxide (H2O2) Assay Kit | \u8ca8\u865fKTB1041 […]<\/p>\n","protected":false},"author":3,"featured_media":27794,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1127],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27796"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27796"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27796\/revisions"}],"predecessor-version":[{"id":44716,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27796\/revisions\/44716"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/27794"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27796"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27796"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27796"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}