{"id":27793,"date":"2021-06-07T11:06:23","date_gmt":"2021-06-07T03:06:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27793"},"modified":"2024-06-28T11:41:39","modified_gmt":"2024-06-28T03:41:39","slug":"purkine-his-tag-cu-ida-resin-%e8%b2%a8%e8%99%9fbmr20040","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27793","title":{"rendered":"PurKine\u2122 His-Tag Cu-IDA Resin | \u8ca8\u865fBMR20040"},"content":{"rendered":"

PurKine\u2122 His-Tag Cu-IDA Resin | <\/strong>\u8ca8\u865f<\/strong>BMR20040<\/a><\/span><\/p>\n

His-tag\uff08\u7d44\u6c28\u9178\u6a19\u7c64\uff09\u7684\u512a\u9ede\uff0c\u7531\u65bc\u7d44\u6c28\u9178\u6a19\u7c64(His-tag)\u5f88\u5c0f\u4e26\u5e7e\u4e4e\u4e0d\u6703\u5e72\u64fe\u76ee\u7684\u86cb\u767d\u7684\u529f\u80fd\u3001\u6d3b\u6027\u6216\u7d50\u69cb\uff0c\u56e0\u6b64\u88ab\u5ee3\u6cdb\u4f7f\u7528\u3002\u56fa\u5b9a\u5316\u91d1\u5c6c\u96e2\u5b50\u89aa\u5408\u5c64\u6790\uff08IMAC\uff09\u662f\u7d14\u5316\u7d44\u6c28\u9178\u6a19\u7c64\u86cb\u767d\u6700\u5e38\u7528\u7684\u65b9\u6cd5\u3002\u9078\u64c7\u6027\u5730\u8ca0\u8f09\u5171\u50f9\u91d1\u5c6c\u96e2\u5b50\uff08\u6bd4\u5982\u93b3\u6216\u9237\uff09\u7684IMAC\u5c64\u6790\u4ecb\u8cea\u80fd\u5920\u622a\u7559\u7d44\u6c28\u9178\u6a19\u7c64\u86cb\u767d\uff0c\u4e26\u5728\u8b8a\u6027\u689d\u4ef6\u4e0b\uff0c\u80fd\u5f9e\u5305\u6db5\u9ad4\u4e2d\u7d14\u5316\u51fa\u4e0d\u6eb6\u6027\u7684\u7d44\u6c28\u9178\u6a19\u7c64\u86cb\u767d\u3002\u6210\u529f\u7684IMAC\u7d14\u5316\u80fd\u5f97\u5230\u9ad8\u7d14\u5ea6\u3001\u9ad8\u7522\u7387\u3001\u9ad8\u6d3b\u6027\u7684\u76ee\u7684\u86cb\u767d\u3002<\/p>\n

\"itanta\"<\/p>\n

PurKine\u2122 His-Tag \u7d14\u5316\u7cfb\u7d71(PurKine\u2122 His-Tag Purification system,\u8ca8\u865fBMR20040) \u57fa\u65bc\u5275\u65b0\u7684\u9ad8\u5bb9\u91cf IMAC \u57fa\u8cea(IMAC matrix)\uff0c\u53ef\u65b9\u4fbf\u5730\u5f9e\u7e3d\u88c2\u89e3\u7269\u4e2d\u4e00\u6b65\u7d14\u5316 His-tag \u86cb\u767d\u3002\u5c08\u6709\u7684\u96e2\u5b50\u87af\u5408\u5316\u5b78\u78ba\u4fdd\u8207\u5e38\u7528\u9084\u539f\u5291\uff08\u5982 DTT\uff09\u3001\u87af\u5408\u91d1\u5c6c\u86cb\u767d\u9176\u6291\u88fd\u5291\uff08\u5982 EDTA\uff09\u4ee5\u53ca\u5404\u7a2e\u7de9\u885d\u7269\u8cea\u548c\u9e7d\u689d\u4ef6\u5177\u6709\u975e\u51e1\u7684\u76f8\u5bb9\u6027\u3002\u8a72\u7522\u54c1\u7d44\u5408\u63d0\u4f9b\u591a\u7a2e\u87af\u5408\u57fa\u5718\uff08IDA\u3001NTA \u6216 Super NTA\uff09\u3001\u4ea4\u806f\u74ca\u8102\u7cd6\uff08\u4ea4\u806f 4% \u6216 6% \u74ca\u8102\u7cd6\uff09\u3001\u96e2\u5b50\uff08Nick\u3001Colbat\u3001Copper \u6216\u975e\u96e2\u5b50\u96fb\u8377\uff09\u548c\u76f8\u5bb9\u6210\u5206\u4ee5\u9032\u884c\u512a\u5316\u6700\u5927\u86cb\u767d\u8cea\u7522\u91cf\u3001\u7a69\u5b9a\u6027\u548c\u6eb6\u89e3\u6027\u7684\u904e\u7a0b\u3002\u6e2c\u8a66\u9084\u8b49\u5be6\uff0c\u5728\u81f3\u5c11\u91cd\u8907\u4f7f\u7528\u4e94\u6b21\u5f8c\uff0c\u6027\u80fd\u4e0d\u6703\u4e0b\u964d\u3002His-tag\u89aa\u548c\u5c64\u6790\u586b\u6599:Ni\u4f5c\u70ba\u6700\u5e38\u7528\u7684\u87af\u5408\u91d1\u5c6c\u96e2\u5b50\uff0c\u8207\u4e4b\u914d\u5957\u7684Resin\u914d\u9ad4\u4e3b\u8981\u70ba\u4e9e\u6c28\u57fa\u4e8c\u4e59\u9178\uff08IDA\uff09\u548c\u6c2e\u5ddd\u4e09\u4e59\u9178\uff08NTA\uff09\u3002Abbkine\u70ba\u60a8\u63d0\u4f9bResin\uff08\u6a39\u8102\u586b\u6599\uff09\uff0cPacked Column\uff08\u9810\u88dd\u7d14\u5316\u67f1\uff09\u548cKit\uff08\u8a66\u5291\u76d2\uff09\u4e09\u7a2e\u5f62\u5f0f\u7522\u54c1\uff0c\u7531\u60a8\u81ea\u7531\u9748\u6d3b\u9078\u64c7~<\/p>\n

 <\/p>\n

Ni-IDA \uff1a <\/span><\/strong>\u5177\u67093\u914d\u4f4d\uff0c\u5f62\u6210\u6bd4\u8f03\u7a69\u5b9a\u7684\u5e73\u9762\u7d50\u69cb\uff0c\u5177\u6709\u8f09\u91cf\u9ad8\uff0c\u6027\u50f9\u6bd4\u9ad8\u7684\u7279\u9ede\uff08\u53ef\u6eb6\u6027\u86cb\u767d\uff09<\/p>\n

Ni-NTA\uff1a<\/span> <\/strong>\u5177\u67094\u914d\u4f4d\uff0c\u5f62\u6210\u975e\u5e38\u7a69\u5b9a\u7684\u516b\u9762\u9ad4\u7d50\u69cb\uff0c\u53ef\u8010\u53d7\u4e00\u5b9a\u6fc3\u5ea6\u7684\u9084\u539f\u5291\u3001\u8b8a\u6027\u5291\u6216\u8026\u5408\u5291\u7b49\u82db\u523b\u689d\u4ef6\uff0c\u9069\u7528\u7bc4\u570d\u66f4\u5ee3\uff01\uff08\u53ef\u6eb6\u6027\u86cb\u767d&\u5305\u6db5\u9ad4\uff09<\/p>\n

Ni-Super\uff1a<\/span><\/strong>\u4e00\u6b3e\u65b0\u578b\u7684\u91d1\u5c6c\u96e2\u5b50\u87af\u5408\u586b\u6599\uff0c\u53ef\u4ee5\u8010\u53d7\u975e\u5e38\u82db\u523b\u7684\u5be6\u9a57\u689d\u4ef6\uff0c \u53ef\u8010\u53d710mM EDTA\u9ad8\u905424h\uff0c\u53efNaOH\u76f4\u63a5\u6e05\u6d17<\/p>\n

6FF\uff1a<\/span><\/strong>\u4ee5\u9ad8\u5ea6\u4ea4\u806f\u76846%\u74ca\u8102\u7cd6\u51dd\u81a0\u70ba\u57fa\u8cea\uff0c\u8010\u53d7\u6700\u9ad80.3 MPa\u7684\u58d3\u529b\uff0c\u53ef\u8207\u8815\u52d5\u6cf5\u3001AKTA\u5100\u5668\u7d50\u5408\u9069\u7528\uff0c\u9069\u5408\u5de5\u696d\u7d14<\/p>\n

Kit \uff1a<\/span><\/strong> \u63d0\u4f9b\u76f8\u61c9\u7684\u91cd\u529b\u9810\u88dd\u67f1\uff08* Packed Column)\u548c\u86cb\u767d\u8cea\u7d14\u5316\u6240\u9700\u7684\u7de9\u885d\u6db2\uff0c\u65b9\u4fbf\u4f7f\u7528\uff0c\u64cd\u4f5c\u7c21\u4fbf\uff0c\u7d14\u5316\u6548\u7387\u9ad8\uff01<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong><\/p>\n

His-Tag Cu-IDA Resin PurKine\u2122 His-Tag Cu-IDA \u6a39\u8102\u53ef\u5728\u5404\u7a2e\u898f\u6a21\u7684\u91cd\u529b\u67f1\u7a0b\u5f0f\u4e2d\u6709\u6548\u7d14\u5316\u9ad8\u6c34\u6e96\u904e\u8868\u9054\u7684 His \u6a19\u7c64\u878d\u5408\u86cb\u767d(overexpressed His-tagged fusion proteins)\u3002\u8a72\u6a39\u8102(Resin)\u7531\u9ad8\u5ea6\u4ea4\u806f\u7684 4% \u74ca\u8102\u7cd6(cross-linked 4% agarose)\u7684 90\u03bcm \u73e0\u5b50\u7d44\u6210\uff0c\u5176\u4e2d\u5df2\u5076\u806f\u4e9e\u6c28\u57fa\u4e8c\u4e59\u9178 (IDA)\u3002\u7136\u5f8c\u87af\u5408\u57fa\u5718(chelating group)\u5e36\u6709\u9285\u96e2\u5b50 (copper ions , Cu2+)\u3002\u6e2c\u8a66\u78ba\u8a8d\u5176\u6027\u80fd\u7b49\u65bc\u6216\u8d85\u904e\u5176\u4ed6\u4f9b\u61c9\u5546 Cu-IDA \u6a39\u8102(Cu-IDA resins)\uff0c\u4e26\u4e14\u5728\u81f3\u5c11\u91cd\u8907\u4f7f\u7528\u4e94\u6b21\u5f8c\u6027\u80fd\u4e0d\u6703\u4e0b\u964d\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u540d\u7a31<\/td>\n\u5f62\u5f0f<\/td>\n\u8ca8\u865f<\/td>\n\u898f\u683c<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Ni-IDA Resin<\/a><\/td>\nResin<\/td>\nBMR20000<\/td>\n5ml\/10ml\/50ml\/100ml<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Ni-NTA Resin<\/a><\/td>\nResin<\/td>\nBMR20010<\/td>\n5ml\/10ml\/50ml\/100ml<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Ni-NTA Packed Column<\/a><\/td>\nPacked Column<\/td>\nBMC20010<\/td>\n1ml*5\/3ml*5<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Protein Purification Kit (Ni-NTA)<\/a><\/td>\nKit<\/td>\nKTP20010<\/td>\n1ml*5\/3ml*5<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Ni-NTA Resin6FF<\/a><\/td>\nResin<\/td>\nBMR20016<\/td>\n5ml\/10ml\/50ml\/100ml<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Ni-NTA Packed Column 6FF<\/a><\/td>\nPacked Column<\/td>\nBMC20016<\/td>\n1ml*5\/3ml*5<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Ni-Super Resin<\/a><\/td>\nResin<\/td>\nBMR20020<\/td>\n5ml\/10ml\/50ml\/100ml<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Ni-Super Packed Column<\/a><\/td>\nPackedColumn<\/td>\nBMC20020<\/td>\n1ml*5\/3ml*5<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Ni-Super Resin 6FF<\/a><\/td>\nResin<\/td>\nBMR20026<\/td>\n5ml\/10ml\/50ml\/100ml<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Ni-Super Packed Column 6FF<\/a><\/td>\nPacked Column<\/td>\nBMC20026<\/td>\n1ml*5\/3ml*5<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Cu-IDA Resin<\/a><\/td>\nResin<\/td>\nBMR20040<\/td>\n5ml\/10ml\/50ml\/100ml<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Co-NTA Resin<\/a><\/td>\nResin<\/td>\nBMR20050<\/td>\n5ml\/10ml\/50ml\/100ml<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag Co-NTA Resin6FF<\/a><\/td>\nResin<\/td>\nBMR20056<\/td>\n5ml\/10ml\/50ml\/100ml<\/td>\n<\/tr>\n
PurKine\u2122\u00a0His-Tag IMAC-NTA Resin 6FF<\/a><\/td>\nResin<\/td>\nBMR20036<\/td>\n5ml\/10ml\/50ml\/100ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n\n\n\n\n\n
Product name<\/strong><\/td>\nPurKine\u2122 His-Tag Cu-IDA Resin<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nPurKine\u2122 His-Tag Cu-IDA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90\u03bcm beads of highly cross-linked 4% agarose, to which Iminodiacetic Acid (IDA) has been coupled. The chelating group has then been charged with copper ions (Cu2+). Tests confirm that performance equals or exceeds popular Cu-IDA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nNi-NTA; His resin; Ni-NTA resin; His tag resin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Product Properties<\/h3>\n\n\n\n\n\n\n\n
Formulation<\/strong><\/td>\nLiquid solution<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 High capacity<\/strong>\u2014More than 50 mg of 6xHis-tagged protein per milliliter of resin.
\n\u2022 Versatile<\/strong>\u2014For purification under native or denaturing conditions.
\n\u2022 Cost-effective<\/strong>\u2014No performance decrease after at least five repeated uses of the same batch of resin.
\n\u2022 Flexible<\/strong>\u2014available in multiple formats including bulk resin, spin columns and complete kits.<\/td>\n<\/tr>\n
Storage buffer<\/strong><\/td>\nLiquid in PBS buffer containing 20% ethanol.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStable for one year at 2-8\u00b0C from date of shipment. Do not freeze.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nBlue ice<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

PurKine\u2122 His-Tag Cu-IDA Resin | \u8ca8\u865fBMR20040 His-tag\uff08\u7d44\u6c28\u9178\u6a19 […]<\/p>\n","protected":false},"author":3,"featured_media":27794,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1128],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27793"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27793"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27793\/revisions"}],"predecessor-version":[{"id":49729,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27793\/revisions\/49729"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/27794"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27793"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27793"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27793"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}