{"id":27610,"date":"2021-05-21T13:55:56","date_gmt":"2021-05-21T05:55:56","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27610"},"modified":"2022-07-19T14:15:31","modified_gmt":"2022-07-19T06:15:31","slug":"20s-%e5%85%8d%e7%96%ab%e8%9b%8b%e7%99%bd%e9%ab%94%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a-%e8%b2%a8%e8%99%9fk2051","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27610","title":{"rendered":"20S \u514d\u75ab\u86cb\u767d\u9ad4\u6d3b\u6027\u6e2c\u5b9a | 20S Immunoproteasome Activity Assay Kit \u8ca8\u865fK2051"},"content":{"rendered":"

20S \u514d\u75ab\u86cb\u767d\u9ad4\u6d3b\u6027\u6e2c\u5b9a | \u00a0\u8ca8\u865f<\/strong>K2051<\/a><\/span><\/p>\n

\"\"<\/p>\n

20S Immunoproteasome Activity Assay Kit (Fluorometric)<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u86cb\u767d\u9176\u9ad4\u4ecb\u8cea\u86cb\u767d\u964d\u89e3(Proteasome mediated protein degradation)\u662f\u7d30\u80de\u4e2d\u53d7\u640d\u3001\u647a\u758a(misfolded\u6216)\u805a\u96c6\u86cb\u767d\u964d\u89e3\u7684\u4e3b\u8981\u9014\u5f91\u4e4b\u4e00\u300226S\u86cb\u767d\u9176\u9ad4\u662f\u964d\u89e3\u6240\u6709\u7121\u8655\u4e0d\u5728\u7684\u86cb\u767d\u8cea\u7684\u86cb\u767d\u9176\u9ad4\u7684\u6700\u5e38\u898b\u5f62\u5f0f\u3002\u5b83\u662f\u4e00\u500b\u4f9d\u8cf4 ATP \u7684\u86cb\u767d\u9176\u8907\u5408\u7269(ATP-dependent proteolytic complex)\uff0c\u5169\u7aef\u5305\u542b\u6876\u5f62\u6838\u5fc3 \uff0820S \u539f\u578b\uff09 \u548c\u5169\u500b\u4e0a\u9650 \uff0819S \u8abf\u7bc0\u8907\u5408\u9ad4\uff09\u300220S\u514d\u75ab\u86cb\u767d\u9176\u9ad4(20S proteasome)\u662f\u86cb\u767d\u9176\u7684\u4e00\u7a2e\u7279\u6b8a\u5f62\u5f0f\uff0c\u5728\u6c27\u5316\u61c9\u6fc0\u6216\u7531\u767c\u708e\u7d30\u80de\u56e0\u6578\u523a\u6fc0\u4e0b\u8868\u9054\u3002\u5728\u6c27\u5316\u61c9\u6fc0\u548c\u708e\u75c7\u904e\u7a0b\u4e2d\uff0c20S \u86cb\u767d\u9176\u6838\u4e2d\u7684\u50ac\u5316\u4e9e\u55ae\u4f4d \u03b21\u3001\u03b12 \u548c \u03b25 \u5206\u5225\u88ab\u4e0d\u53ef\u53d7\u8a98\u60d1\u7684\u4e9e\u55ae\u4f4d \u03b21i\/LMP2\u3001\u03b22i\/MECL1 \u548c \u03b25i\/LMP7 \u6240\u53d6\u4ee3\u3002\u9019\u4f7f\u5f9720S\u514d\u75ab\u86cb\u767d\u9ad4\u80fd\u5920\u4ee5\u4e0d\u540c\u7684\u65b9\u5f0f\u5207\u5272\u86cb\u767d\u8cea\uff0c\u820720S\u86cb\u767d\u9ad4\u76f8\u6bd4\uff0c\u7531\u65bc\u8f49\u5411\u985e\u4f3c\u808c\u9ef4\u7d20\u7684\u50ac\u5316\u6d3b\u6027\u3002\u6b64\u5916\uff0c\u9019\u4e9b\u8a98\u5c0e\u50ac\u5316\u4e9e\u55ae\u4f4d\u5728\u901a\u904e\u4e3b\u8981\u7d44\u7e54\u76f8\u5bb9\u6027I\u985e\u901a\u8def\u5448\u73fe\u7684\u65b0\u578b\u6297\u539f\u80bd\u7684\u7522\u751f\u4e2d\u767c\u63ee\u8457\u91cd\u8981\u4f5c\u7528\u3002BioVision \u7684 20S \u514d\u75ab\u86cb\u767d\u9ad4\u6d3b\u52d5\u6aa2\u6e2c\u5957\u4ef6\u4f7f\u7528\u57fa\u65bc\u677f\u7684\u6c1f\u6e2c\u5b9a\u6e2c\u8a66\u4f86\u6e2c\u91cf 20S \u514d\u75ab\u86cb\u767d\u9ad4\u7684\u6d3b\u6027\u3002\u5728\u672c\u6b21\u6aa2\u6e2c\u4e2d\uff0c20S\u514d\u75ab\u86cb\u767d\u9ad4\u5207\u958b\u87a2\u5149\u57fa\u8cea\uff0c\u91cb\u653e\u51fa7-\u6c28\u57fa-4-\u7532\u57fa\u4e19\u6c28\u57fa\uff0c\u9019\u662f\u4e00\u7a2e\u87a2\u5149\u5316\u5408\u7269\uff0c\u5728Ex\/Em=345\/445 nm\u7522\u751f\u7a69\u5b9a\u4fe1\u865f\u3002\u8a72\u5957\u4ef6\u53ef\u4ee5\u5728\u6aa2\u6e2c\u689d\u4ef6\u4e0b\u6aa2\u6e2c\u4f4e\u81f3 0.02\u03bcU \uff08\u6bcf\u5206\u9418 0.02 pmol \u57fa\u8cea\u86cb\u767d\u9176\uff09 \u7684 20S \u514d\u75ab\u86cb\u767d\u9ad4\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK2051-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nBioVision’s 20S Immunoproteasome Activity Assay Kit uses a plate-based fluorometric assay to measure the activity of the 20S immunoproteasome. In this assay, the 20S immunoproteasome cleaves a fluorogenic substrate to release 7-amino-4-methylcoumarin, a fluorescent compound that produces a stable signal at Ex\/Em = 345\/445 nm. The kit can detect as low as 0.02 \u00b5U (0.02 pmol substrate proteolysis per minute) of 20S immunoproteasome under assay conditions.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em = 345\/445 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nTo measure the activity of 20S immunoproteasome in samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 A plate-based fluorometric assay to measure the activity of the 20S immunoproteasome.
\n\u2022 The kit can detect as low as 0.02 \u00b5U (0.02 pmol substrate proteolysis per minute) of 20S immunoproteasome under assay conditions.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 20S IP Assay Buffer
\n\u2022 20S IP Substrate
\n\u2022 20S IP Positive Control
\n\u2022 AMC Standard
\n\u2022 20S IP Inhibitor<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00baC<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

20S \u514d\u75ab\u86cb\u767d\u9ad4\u6d3b\u6027\u6e2c\u5b9a | \u00a0\u8ca8\u865fK2051 20S Immunoproteasome Activity […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1135],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27610"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27610"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27610\/revisions"}],"predecessor-version":[{"id":27613,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27610\/revisions\/27613"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27610"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27610"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27610"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}