{"id":27311,"date":"2021-05-04T15:28:47","date_gmt":"2021-05-04T07:28:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27311"},"modified":"2022-07-19T14:17:46","modified_gmt":"2022-07-19T06:17:46","slug":"%e8%b3%aa%e8%86%9c%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-plasma-membrane-isolation-kit-%e8%b2%a8%e8%99%9fk414","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27311","title":{"rendered":"\u8cea\u819c\u5206\u96e2\u8a66\u5291\u76d2 | Plasma Membrane Isolation Kit \u8ca8\u865fK414"},"content":{"rendered":"

\u8cea\u819c\u5206\u96e2\u8a66\u5291\u76d2 | \u00a0\u8ca8\u865f<\/strong>K414<\/a><\/span><\/p>\n

OrgFrontier\u2122<\/em><\/strong> Plasma Membrane Isolation Kit<\/em><\/strong><\/p>\n

\"\"\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u8cea\u819c(Plasma membrane)\u662f\u7814\u7a76\u6700\u591a\u7684\u7d30\u80de\u819c(cellular membranes)\u4e4b\u4e00\u3002\u539f\u6838\u751f\u7269(Prokaryotic organisms)\u548c\u771f\u6838\u751f\u7269(eukaryotic organisms)\u5177\u6709\u8cea\u819c\uff0c\u7e3d\u7684\u4f86\u8aaa\uff0c\u7531\u65bc\u5b83\u5011\u7684\u534a\u6ef2\u900f\u6027\u7279\u5fb5(semi-permeable characteristics)\uff0c\u5b83\u5011\u8207\u5468\u570d\u74b0\u5883\u76f8\u901a\u7684\u540c\u6642\u5305\u88f9\u4e26\u4fdd\u8b77\u4e86\u7d30\u80de\u8cea\u548c\u7d30\u80de\u5668\u3002\u8cea\u819c\u662f\u9078\u64c7\u6027\u6ef2\u900f\u7684\u8102\u8cea\u96d9\u5c64(lipid bilayers)\uff1a\u5b83\u5011\u5141\u8a31\u71df\u990a\u7269\u8cea\u548c\u5176\u4ed6\u4ee3\u8b1d\u7269\u904b\u8f38\u5230\u7d30\u80de\u8cea\u4e2d\uff0c\u4e26\u5141\u8a31\u540c\u6642\u8f38\u51fa\u7d30\u80de\u5ee2\u7269\u3002\u6c27\u6c23\uff0c\u4e8c\u6c27\u5316\u78b3\u548c\u5176\u4ed6\u6c23\u614b\u6216\u89aa\u8102\u6027\u5c0f\u5206\u5b50\u5728\u8cea\u819c\u4e0a\u81ea\u7531\u64f4\u6563\uff1b\u7136\u800c\uff0c\u96e2\u5b50\uff0c\u6c28\u57fa\u9178\u548c\u91a3\u985e\u9075\u5faa\u56b4\u683c\u7684\u88ab\u52d5\u548c\u4e3b\u52d5\u7d30\u80de\u904b\u8f38\u6a5f\u5236\u3002\u8cea\u819c\u8102\u8cea\u96d9\u5c64\u4e3b\u8981\u7531\u56db\u7a2e\u78f7\u8102\u7d44\u6210\uff1a\u78f7\u8102\u81bd\u9e7c(phosphatidylcholine)\uff0c\u78f7\u8102\u4e59\u9187\u80fa(phosphatidylethanolamine)\uff0c\u78f7\u8102\u6c28\u9178(phosphatidylserine)\u548c\u9798\u78f7\u8102(sphingomyelin)\u3002\u78f7\u8102\u808c\u9187\uff0c\u7b2c\u4e94\u7a2e\u78f7\u8102\uff0c\u4e5f\u4f4d\u65bc\u819c\u7684\u5167\u5074\u3002\u53e6\u5916\uff0c\u8a31\u591a\u86cb\u767d\u8cea\u88ab\u5d4c\u5165\u6216\u767c\u73fe\u7c98\u9644\u5728\u8cea\u819c\u7684\u5169\u5074\u3002\u4f8b\u5982\uff0c\u901a\u5e38\u767c\u73fe\u7cd6\u86cb\u767d\u9644\u8457\u5728\u819c\u7684\u5916\u5074\u3002 BioVision\u7684OrgFrontier\u2122\u8840\u6f3f\u819c\u5206\u96e2\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u5957\u5c08\u6709\u7684\u8a66\u5291\u548c\u7de9\u885d\u6db2\uff0c\u65e8\u5728\u512a\u5316\u5f9e\u54fa\u4e73\u52d5\u7269\u7d44\u7e54\u548c\u7d30\u80de\u4e2d\u63d0\u53d6\u548c\u5206\u96e2\u5b8c\u6574\u7684\u8cea\u819c\u3002\u9700\u8981\u57f7\u884c\u8d85\u901f\u96e2\u5fc3\u6a5f\uff0c\u4e26\u4e14\u53ef\u4ee5\u5728\u5e7e\u500b\u5c0f\u6642\u5167\u5b8c\u6210\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK414-10<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n10 preparations<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Isolation of plasma membrane fractions from tissues and cultured cells.
\n\u2022 Plasma membrane studies and plasma membrane protein profiling.
\n\u2022 Enrichment of membrane-associated proteins for western blot and ELISA<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Isolation of plasma membrane fractions from tissues and cultured cells.
\n\u2022 Plasma membrane studies and plasma membrane protein profiling.
\n\u2022 Enrichment of membrane-associated proteins for western blot and ELISA<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure
\n\u2022 Fast and convenient
\n\u2022 The Membrance Protein Extraction Kit provides an easy and complete protocol for isolating integral membrance proteins efficiently from cultured mammalian cells.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Homogenization Buffer
\n\u2022 EZBlock\u2122 Protease Inhibitor Cocktail
\n\u2022 Gradient Dilution Buffer
\n\u2022 OptiPrep\u2122 Density Gradient Medium<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7522\u54c1\u554f\u8207\u7b54<\/p>\n

\u6211\u5011\u5efa\u8b70\u4f7f\u7528\u64fa\u52d5\u93df\u6597\u8f49\u5b50(swinging bucket rotor)\u3002\u4f46\u662f\uff0c\u6211\u5011\u9084\u6e2c\u8a66\u4e86\u5b9a\u89d2\u8f49\u5b50\uff08Timan 45\u6216Ti 50\uff0cBeckman Coulter\uff09\uff0c\u5b83\u4e5f\u53ef\u4ee5\u6b63\u5e38\u5de5\u4f5c\u3002<\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Masuda T, Large-Scale Quantitative Comparison of Plasma Transmembrane Proteins between Two Human Blood-Brain Barrier Model Cell Lines, hCMEC\/D3 and HBMEC\/ci\u03b2.\u00a0Mol Pharm, June 2019; 30983357.<\/a><\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
 <\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u8cea\u819c\u5206\u96e2\u8a66\u5291\u76d2 | \u00a0\u8ca8\u865fK414 OrgFrontier\u2122 Plasma Membrane Isolatio […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1140],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27311"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27311"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27311\/revisions"}],"predecessor-version":[{"id":27312,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27311\/revisions\/27312"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27311"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27311"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27311"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}