{"id":27236,"date":"2021-04-23T11:10:21","date_gmt":"2021-04-23T03:10:21","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27236"},"modified":"2022-07-19T14:31:34","modified_gmt":"2022-07-19T06:31:34","slug":"minute%e6%96%b0%e9%ae%ae%e5%86%b7%e5%87%8d%e7%b5%84%e7%b9%94%e8%83%9e%e8%b3%aa%e8%83%9e%e6%a0%b8%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9f-nt-032","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27236","title":{"rendered":"Minute\u2122\u65b0\u9bae\/\u51b7\u51cd\u7d44\u7e54\u80de\u8cea\u80de\u6838\u5206\u96e2\u8a66\u5291\u76d2 | \u8ca8\u865f NT-032"},"content":{"rendered":"

Minute\u2122<\/strong>\u65b0\u9bae<\/strong>\/<\/strong>\u51b7\u51cd\u7d44\u7e54\u80de\u8cea\u80de\u6838\u5206\u96e2\u8a66\u5291\u76d2<\/strong> | <\/strong>\u8ca8\u865f<\/strong> NT-032<\/strong><\/a><\/span><\/p>\n

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Minute\u2122 Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tis<\/strong>sues <\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u5f9e\u7d30\u80de\u548c\u7d44\u7e54\u4e2d\u5206\u96e2\u7d30\u80de\u8cea(cytosolic)\u548c\u7d30\u80de\u6838\u7d44\u5206(nuclear fractions)\u662f\u4e00\u7a2e\u5e38\u898b\u7684\u5be6\u9a57\u3002\u7d30\u80de\u6a23\u54c1\u7684\u7d44\u5206\u5206\u96e2\u76f8\u5c0d\u7c21\u55ae\u76f4\u63a5\uff0c\u7136\u800c\uff0c\u5f9e\u7d44\u7e54\u6a23\u54c1\u4e2d\u7279\u5225\u662f\u51b7\u51cd\u7d44\u7e54(frozen tissues)\u4e2d\u5c07\u80de\u8cea\u548c\u80de\u6838\u7d44\u5206\u5206\u96e2\u4e7e\u6de8\u662f\u5f88\u5177\u6311\u6230\u7684\u3002\u56e0\u70ba\u51b7\u51cd\u548c\u53cd\u5fa9\u51cd\u878d\u6703\u5f15\u8d77\u7d44\u7e54\u7d30\u80de\u7d50\u69cb\u7684\u6539\u8b8a\uff0c\u52a0\u4e4b\u4e0d\u9069\u7576\u7684\u52fb\u6f3f\u65b9\u5f0f\u548c\u6548\u7387\u4f4e\u7684\u63d0\u53d6\u7de9\u885d\u6db2\uff0c\u4f7f\u5f97\u4ea4\u53c9\u6c61\u67d3\u5341\u5206\u56b4\u91cd\u3002\u76ee\u524d\u5927\u591a\u6578\u5546\u696d\u8a66\u5291\u76d2\u8a2d\u8a08\u53ef\u540c\u6642\u7528\u65bc\u7d30\u80de\u548c\u7d44\u7e54\u6a23\u54c1\uff0c\u4f46\u662f\u5ffd\u7565\u4e86\u5169\u7a2e\u985e\u578b\u6a23\u54c1\u4e4b\u9593\u7684\u7d50\u69cb\u5dee\u7570\u3002\u56e0\u6b64\uff0c\u5f9e\u65b0\u9bae\/\u51b7\u51cd\u7d44\u7e54\u4e2d\u5206\u96e2\u80de\u8cea\u548c\u80de\u6838\u7d44\u5206\u6642\uff0c\u4ea4\u53c9\u6c61\u67d3\u554f\u984c\u7121\u6cd5\u907f\u514d(\u898b\u4e0b\u6587\u53c3\u8003\u6587\u737b1\u548c2)\u3002\u9019\u6b3e\u65b0\u578b\u7684\u80de\u8cea\u548c\u80de\u6838\u5206\u96e2\u8a66\u5291\u76d2\u5c08\u7528\u65bc\u7d44\u7e54\u6a23\u54c1\u7684\u80de\u8cea\u80de\u6838\u5206\u96e2\uff0c\u8a66\u5291\u76d2\u7279\u6709\u7684\u7de9\u885d\u6db2\u9ad4\u7cfb\u548c\u7368\u7279\u7684\u64cd\u4f5c\u65b9\u6cd5\u53ef\u4ee5\u5c07\u4ea4\u53c9\u6c61\u67d3\u964d\u5230\u6700\u4f4e\u3002\u6574\u500b\u64cd\u4f5c\u53ef\u4ee5\u572830\u5206\u9418\u5167\u5b8c\u6210\u3002<\/p>\n

\"\"<\/p>\n

\u61c9\u7528\uff1a<\/p>\n

\u53ef\u61c9\u7528\u65bcSDS-PAGE\uff0cimmunoblottings\uff0cELISA\uff0cIP\uff0c\u86cb\u767d\u5b9a\u4f4d\uff0c\u51dd\u81a0\u9077\u79fb\u5206\u6790\uff0c2-D\u7b49\u5176\u4ed6\u61c9\u7528\u3002<\/p>\n

\u5132\u5b58\uff1a<\/p>\n

4\u2103<\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

1.Li, Y., Wu, J., Yu, S., Zhu, J., Zhou, Y., Wang, P., … & Zhao, Y. (2020). Sestrin2 promotes angiogenesis to alleviate brain injury by activating Nrf2 through regulating the interaction between p62 and Keap1 following photothrombotic stroke in rats.Brain Research, 146948.<\/p>\n

2.Wang, B., Huang, X., Pan, X., Zhang, T., Hou, C., Su, W. J., … & Wang, Y. X. (2020). Minocycline prevents the depressive-like behavior through inhibiting the release of HMGB1 from microglia and neurons.Brain, Behavior, and Immunity.<\/p>\n

3.Song T, Guan X, Wang X,et al. Dynamic modulation of gut microbiota improves post-myocardial infarct tissue repair in rats via butyric acid-mediated histone deacetylase inhibition.The FASEB Journal. 2021;35:e21385.<\/p>\n

4.Zhou, J., Zhao, Y., Guo, Y. J., Zhao, Y. S., Liu, H., Ren, J., … & Ji, E. S. (2021). A rapid juvenile murine model of nonalcoholic steatohepatitis (NASH): Chronic intermittent hypoxia exacerbates Western diet-induced NASH. Life Sciences, 119403.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/u><\/td>\nCatalog No.<\/u><\/td>\n<\/tr>\n
\u3000<\/u><\/td>\n\u3000<\/u><\/td>\n<\/tr>\n
Minute TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\nSD-001\/SN-002<\/td>\n<\/tr>\n
Minute TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\nSC-003<\/td>\n<\/tr>\n
Minute TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\nSN-006<\/td>\n<\/tr>\n
Minute TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\nSM-005<\/td>\n<\/tr>\n
Minute TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\nSM-005-p<\/td>\n<\/tr>\n
Minute TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\nMP-007<\/td>\n<\/tr>\n
Minute TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests) NEW!<\/td>\nMM-038<\/td>\n<\/tr>\n
Minute TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\nSD-008\/SN-009<\/td>\n<\/tr>\n
Minute TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\nSN-010<\/td>\n<\/tr>\n
Minute TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\nCP-011<\/td>\n<\/tr>\n
Minute TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\nSB-004<\/td>\n<\/tr>\n
Minute TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\nSC-012<\/td>\n<\/tr>\n
Minute TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\nNE-013<\/td>\n<\/tr>\n
Minute TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\nHP-014<\/td>\n<\/tr>\n
Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYT-015<\/td>\n<\/tr>\n
Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYD-016<\/td>\n<\/tr>\n
Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\nYM-017<\/td>\n<\/tr>\n
Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\nMM-018<\/td>\n<\/tr>\n
Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\nPN-019<\/td>\n<\/tr>\n
MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\nHD-021<\/td>\n<\/tr>\n
MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\nAT-022<\/td>\n<\/tr>\n
MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\nAF-023<\/td>\n<\/tr>\n
MinuteTM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\nNI-024<\/td>\n<\/tr>\n
MinuteTM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps) NEW!<\/td>\nBN-020<\/td>\n<\/tr>\n
MinuteTM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps) NEW!<\/td>\nSN-047<\/td>\n<\/tr>\n
MinuteTM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\nFE-025<\/td>\n<\/tr>\n
MinuteTM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\nEI-027<\/td>\n<\/tr>\n
MinuteTM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\nSE-030<\/td>\n<\/tr>\n
MinuteTM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\nNT-032<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\nLY-034<\/td>\n<\/tr>\n
MinuteTM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\nER-036<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-039<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-042<\/td>\n<\/tr>\n
MinuteTM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPT-040<\/td>\n<\/tr>\n
MinuteTM<\/sup> Nuclear Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPN-041<\/td>\n<\/tr>\n
MinuteTM<\/sup> Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests) NEW!<\/td>\nPF-045<\/td>\n<\/tr>\n
MinuteTM<\/sup> Native Protein Precipitation Kit from Saliva (20 tests) NEW!<\/td>\nSV-043<\/td>\n<\/tr>\n
MinuteTM<\/sup> Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) NEW!<\/td>\nML-044<\/td>\n<\/tr>\n
MinuteTM<\/sup> Rubisco Depletion Kit (20 tests) NEW!<\/td>\nRD-046<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Skin Tissue (50 tests)<\/td>\nSA-01-SK<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Bone Tissue (50 tests)<\/td>\nSA-02-BT<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Blood Vessels (50 tests)<\/td>\nSA-03-BV<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Tendons (50 tests)<\/td>\nSA-04-TD<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Intestines and Mesentery (50 tests)<\/td>\nSA-05-IM<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Muscles (50 tests)<\/td>\nSA-06-MS<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Insects (50 tests)<\/td>\nSA-07-IS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1 \u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | \u8cea\u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a> |<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Minute\u2122\u65b0\u9bae\/\u51b7\u51cd\u7d44\u7e54\u80de\u8cea\u80de\u6838\u5206\u96e2\u8a66\u5291\u76d2 | \u8ca8\u865f NT-032 Minute\u2122 Cytosolic a […]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1144],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27236"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27236"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27236\/revisions"}],"predecessor-version":[{"id":27239,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27236\/revisions\/27239"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27236"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27236"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}