{"id":27229,"date":"2021-04-20T13:27:06","date_gmt":"2021-04-20T05:27:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27229"},"modified":"2026-05-25T14:22:05","modified_gmt":"2026-05-25T06:22:05","slug":"chekine%e6%b4%bb%e6%80%a7%e6%b0%a7%ef%bc%88ros%ef%bc%89%e6%aa%a2%e6%b8%ac%e5%88%86%e6%9e%90-reactive-oxygen-species-ros-detection-assay-kit-%e8%b2%a8%e8%99%9fktb1910","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27229","title":{"rendered":"ROS\u6d3b\u6027\u6c27\uff08ROS\uff09\u6aa2\u6e2c\u5206\u6790 | Reactive Oxygen Species (ROS) Detection Assay Kit \u8ca8\u865fKTB1910"},"content":{"rendered":"

CheKine\u2122\u6d3b\u6027\u6c27\uff08ROS\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09CheKine\u2122 Reactive Oxygen Species (ROS) Detection Assay Kit\u00a0| \u8ca8\u865fKTB1910<\/a><\/span><\/strong><\/span><\/h4>\n

CheKine\u2122 Reactive Oxygen Species (ROS) Detection Assay Kit (Fluorometric)\u00a010 mM\u00a0 50\u500b\u53cd\u61c9(\u00a010mM, 50ul)\/100\u500b\u53cd\u61c9 (10mM, 100ul)\u00a0 (\u4ee56well, 1ml\u9ad4\u7a4d\u70ba\u4e00\u500b\u53cd\u61c9)<\/p>\n

\u6d3b\u6027\u6c27\u7269\u7a2e(Reactive Oxygen Species, Ros ) Ros\u662f\u6c27\u6b63\u5e38\u4ee3\u8b1d\u7684\u5929\u7136\u526f\u7522\u7269\uff0c\u5728\u7d30\u80de\u4fe1\u865f\u50b3\u5c0e(cell signaling)\u548c\u9ad4\u5167\u5e73\u8861(vivo balance)\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 \u4f46\u662f\uff0c\u5728\u6c27\u5316\u61c9\u6fc0\u76f8\u95dc\u72c0\u614b(oxidative stress correlation state)\u4e0b\uff0cROS\u6c34\u5e73\u53ef\u4ee5\u5927\u5927\u63d0\u9ad8\u3002 ROS\u7684\u7a4d\u7d2f\u5c07\u56b4\u91cd\u7834\u58de\u7d30\u80de\u7d50\u69cb(cell structure)\u3002 \u6c27\u5316\u61c9\u6fc0(Oxidative stress)\u5728\u5fc3\u8840\u7ba1\u75be\u75c5(cardiovascular disease)\uff0c\u7cd6\u5c3f\u75c5(diabetes)\uff0c\u9aa8\u8cea\u758f\u9b06(osteoporosis)\uff0c\u4e2d\u98a8(stroke)\uff0c\u708e\u6027\u75be\u75c5(inflammatory disease)\u4ee5\u53ca\u795e\u7d93\u9000\u884c\u6027\u75be\u75c5(neurodegenerative diseases)\u548c\u764c\u75c7(cancer)\u7b49\u7814\u7a76\u4e2d\u8d77\u8457\u91cd\u8981\u4f5c\u7528\u3002 ROS\u6aa2\u6e2c\u5c07\u6709\u52a9\u65bc\u78ba\u5b9a\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u5982\u4f55\u8abf\u7bc0\u5404\u7a2e\u7d30\u80de\u5167\u9014\u5f91(intracellular pathways)\u3002<\/p>\n

\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> <\/span><\/span><\/p>\n

CheKine\u2122\u6d3b\u6027\u6c27\uff08CheKine\u2122 Reactive Oxygen Species , ROS\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u5feb\u901f\u7684ROS\u6aa2\u6e2c\u65b9\u6cd5\u3002 ROS\u6aa2\u6e2c\u57fa\u65bc\u87a2\u5149\u63a2\u91ddDCFH-DA\u3002 DCFH-DA\u662f\u4e00\u7a2e\u53ef\u6ef2\u900f\u7d30\u80de\u7684\u9748\u654f\u63a2\u91dd\uff0c\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u5167\u6d3b\u6027\u6c27\uff08reactive oxygen species , ROS\uff09\u3002 \u8a72DCFH-DA\u63a2\u91dd\u53ef\u5728\u7a7f\u904e\u6d3b\u7d30\u80de\u819c\u6642\u88ab\u7d30\u80de\u4e2d\u7684\u916f\u9176\u6c34\u89e3\u70baDCFH\u3002 DCFH\u6c92\u6709\u87a2\u5149\u4e26\u4e14\u4e0d\u80fd\u900f\u904e\u7d30\u80de\u819c\uff0c\u7136\u5f8c\u53ef\u4ee5\u88abROS\u6c27\u5316\u4e26\u7522\u751f\u87a2\u5149DCF\u3002 \u7136\u5f8c\u53ef\u4ee5\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u6aa2\u6e2c\u87a2\u5149\uff0c\u4e26\u64da\u6b64\u5206\u6790\u7d30\u80de\u4e2d\u6d3b\u6027\u6c27\uff08CheKine\u2122 Reactive Oxygen Species , ROS\uff09\u7684\u542b\u91cf\u3002<\/p>\n

<\/p>\n

\u5f15\u7528\u6587\u737b<\/span><\/a><\/p>\n

Astragaloside alleviates alcoholic fatty liver disease by suppressing oxidative stress<\/p>\n

A rat ROS ELISA kit was purchased from Abbkine and used to analyze the concentration of ROS. AML-12 cells and rat liver tissues were homogenized and centrifuged, and supernatants were collected. Ten microliters of supernatant was mixed with 40 \u03bcl of diluent and added to each well. Plates were incubated at 37\u0001C for 50 min. Each well was washed five times with 250 \u03bcl of wash buffer. The HRP-conjugated detection antibody was added and incubated at 37\u0001C for 30 min. After washing, the chromogen solution mixture was added and incubated at 37\u0001C for 15 min. Then, 50 \u03bcl of stop solution was added. Optical density at 450 nm was read immediately. For superoxide dismutase (SOD), collected AML-12 and liver tissue lysates were used to assess SOD concentrations. Twenty microliters of lysates were mixed with working solution and incubated for 20 min at 37\u0001C. Absorbance was then recorded at 450 nm.<\/p>\n

\"\"<\/p>\n

Analysis of ROS and superoxide dismutase (SOD) levels in different groups (n = 3).<\/p>\n

<\/p>\n

Reactive Oxygen Species (ROS) level in MDA-MB-231 cells measured through 2\u2032,7\u2032-Dichlorofluorescein diacetate (DCFH-DA) at 24 h after rapamycin treatment.<\/p>\n

\"\u64cd\u4f5c\u65b9\u6cd5\"<\/a><\/p>\n\n\n\n\n\n\n\n\n
Kit components<\/strong><\/td>\n\u2022 DCFH-DA (10 mM)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 provides a simple, sensitive, rapid ROS detection method.
\n\u2022 based on the fluorescent probe DCFH-DA .
\n\u2022 suitable for flow cytometry or fluorescence microscopy.
\n\u2022 Provides detailed sample preparation and results calculation methods.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
\n\u2022 Avoid bubbles while mixing or redissolving components.
\n\u2022 Change pipette tips frequently to avoid cross contamination between components.
\n\u2022 Ensure that all components and equipment are at the proper temperature before starting the experiment.
\n\u2022 The working concentration of positive control H2O2 should be 100 \u03bcM (Recommend during 50-200 \u03bcM depend on the cell types ). The level of Reactive Oxygen Species (ROS) will increase significantly after stimulated 30 min-4 h. The level of Reactive Oxygen Species (ROS) maybe very different for different cells while stimulating with the positive control. The stimulating time and the concentration of positive control could be prolonged or increased, if there is no obvious rise after 30 min . Or shorten the time or reduce the concentration of positive control, while the fluorescent signal is too strong.
\n\u2022 During the experiment, the fluorescent probe DCFH-DA can be diluted in 1: 2000-1: 5000, and make the final concentration of DCFH-DA to 2-5 \u03bcM if the signal of negative control also too strong. The time of probe incubating can be adjusted in 15-60 minutes, and minimize the exposure time while reading the film, and the exposure time of the control group and test groups should to be consistent.
\n\u2022 Reactive Oxygen Species (ROS) positive control (H2O2) is only used as a sample as a positive control, and not all test groups need positive control.
\n\u2022 Please wash away the unbound prode after incubating the prode , otherwise it will cause a high background.
\n\u2022 Try to shorten the detection time after incubating the probe (except for stimulation time) to reduce various possible errors.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nPlease store the whole kit at -20\u00b0C upon receipt ,and protect from light. The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

BRAND\/AGENCY\/\u53f0\u7063\u4ee3\u7406\u5546<\/span>\/\u00a0<\/span>Abbkine\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/span><\/strong><\/h2>\n

Abbkine Scientific Co., Ltd\u7531\u4e00\u7fa4\u5fd7\u540c\u9053\u5408\u7684\u79d1\u5b78\u5bb6\u548c\u884c\u92b7\u5c08\u5bb6\uff0c\u57282012\u5e74\u6210\u7acb\u65bc\u7f8e\u570b\u52a0\u5229\u798f\u5c3c\u4e9e\u5dde\uff0c\u96a8\u8457\u4e9e\u6d32\u5e02\u5834\u7684\u8fc5\u731b\u589e\u9577\uff0cAbbkine\u5c07\u7e3d\u90e8\u642c\u9077\u81f3\u4e2d\u570b\u3002\u7d50\u5408\u4e86Abbkine\u7f8e\u570b\u6280\u8853\u548c\u4e2d\u570b\u88fd\u9020\u5169\u65b9\u9762\u7684\u5de8\u5927\u512a\u52e2\uff0cAbbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\uff0c\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002<\/p>\n

\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"\"abbkine02\"\u00a0\"\u8840\u6e05\"<\/p>\n

\u767c\u5c55\u6b77\u7a0b:\u00a0<\/strong>Abbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002Abbkine\u4ee5\u7279\u8272\u7684\u86cb\u767d\u8cea\u751f\u7522\u8207\u6aa2\u6e2c\u8a66\u5291\u8d77\u5bb6\uff0c\u81f4\u529b\u65bc\u5275\u65b0\u548c\u7814\u767c\u5404\u985e\u6297\u9ad4\u3001\u751f\u5316\u8a66\u5291\u3001\u86cb\u767d\u8cea\u548c\u5be6\u9a57\u8a66\u5291\u76d2\uff0c\u4ee5\u671f\u671b\u70ba\u5168\u7403\u79d1\u7814\u5de5\u4f5c\u8005\u63d0\u4f9b\u9769\u65b0\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6211\u5011\u5f88\u81ea\u8c6a\u5730\u70ba\u5ba2\u6236\u63d0\u4f9b\u5982\u4e0b\u7279\u8272\u7522\u54c1\u7dda\uff0c\u4e26\u6703\u4e00\u5982\u65e2\u5f80\u5730\u958b\u767c\u65b0\u578b\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u751f\u547d\u79d1\u5b78\u7814\u7a76\u548c\u8a3a\u65b7\u8a66\u5291\u7522\u54c1\u3002<\/p>\n

2017\u5e74\u6aa2\u6e2c\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u7684ELISA\u8a66\u5291\u76d2\u61c9\u904b\u800c\u751f: \u6839\u64da\u5e02\u5834\u9700\u6c42\uff0c\u958b\u767cEliKine\u2122\u7cfb\u5217ELISA\u8a66\u5291\u76d2\uff0c\u6db5\u84cb\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u71b1\u9580\u6307\u6a19\uff0c\u5982\u767d\u4ecb\u7d20\uff08IL\uff09\u3001\u816b\u7624\u58de\u6b7b\u56e0\u6578\uff08TNF\uff09\u3001\u5e72\u64fe\u7d20\uff08IFN\uff09\u7b49\u660e\u661f\u7d1a\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\u5168\u7403\u767c\u552e:2018\u5e74\u6191\u85c9\u5148\u9032\u7684\u86cb\u767d\u8cea\u5076\u806f\u6280\u8853\u548c\u56b4\u683c\u7684\u54c1\u8cea\u63a7\u5236\uff0c\u6211\u5011\u63d0\u4f9bLinKine\u2122\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\uff0c\u5728\u6297\u9ad4\u3001\u86cb\u767d\u8cea\u3001\u591a\u80bd\u6216\u8005\u5176\u5b83\u751f\u7269\u5206\u5b50\u4e0a\u53ef\u76f4\u63a5\u6a19\u8a18HRP\uff0cBiotin\uff0cFITC\uff0cCy3\uff0cAbFluor\u2122\u4ee5\u6eff\u8db3\u79d1\u7814\u61c9\u7528\uff0c\u85e5\u7269\u7814\u767c\u4ee5\u53ca\u8a3a\u65b7\u8a66\u5291\u76d2\u958b\u767c\u7684\u9700\u6c42\u30022019\u5e74\u751f\u5316\u3001\u7d30\u80de\u985e\u7522\u54c1\u7dda\u9010\u6b65\u64f4\u5145\u642d\u5efa\u7d30\u80de\u7522\u54c1\u7814\u767c\u5e73\u81fa\uff0c\u7d30\u80de\u72c0\u614b\u6aa2\u6e2c\u3001\u7d30\u80de\u67d3\u8272\u3001\u7d30\u80de\u63d0\u53d6\u3001\u751f\u5316\u8a66\u5291\u76d2\u7522\u54c1\u5f97\u5230\u8d8a\u4f86\u8d8a\u591a\u5168\u7403\u5ba2\u6236\u7684\u8a8d\u53ef\u3002<\/p>\n

    \n
  1. \u5168\u5957\u8868\u4f4d\u6a19\u7c64\u6297\u9ad4\u548c\u5167\u53c3\u6297\u9ad4\uff0c\u4ee5\u53ca\u76f8\u61c9\u7684\u5b9a\u5236\u5076\u806f\u7522\u54c1<\/li>\n
  2. \u5c0f\u5305\u88dd\u7684\u78f7\u9178\u5316\u548c\u7532\u57fa\u5316\u7279\u7570\u6027\u7684\u6297\u9ad4\u7522\u54c1<\/li>\n
  3. \u6700\u71b1\u9580\u7684\u4e8c\u6297\u7522\u54c1\uff1a\u7528\u65bcIF\u7684IFKine\u7cfb\u5217\u7522\u54c1\uff0c\u4ee5\u53caIP-WB\u7684IPKine\u7cfb\u5217\u7522\u54c1<\/li>\n
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  5. \u66a2\u92b7\u7684\u91cd\u7d44\u86cb\u767d\uff0c\u5305\u542b\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u751f\u9577\u56e0\u6578<\/li>\n
  6. \u66f4\u591a\u7d30\u80de\u529f\u80fd\u5206\u6790\u8a66\u5291\u76d2\uff0cELISA\u8a66\u5291\u76d2\uff0c\u4ee5\u53ca\u7d30\u80de\u793a\u8e64\u8a66\u5291<\/li>\n<\/ol>\n

    \u6b64\u5916\uff0cAbbkine\u9084\u5efa\u7acb\u4e86\u6280\u8853\u670d\u52d9\u5e73\u81fa\uff0c\u5305\u62ec\u514d\u75ab\u5b78\u76f8\u95dc\u5be6\u9a57\u670d\u52d9\uff08WB\/IHC\/IF\/IP\uff09\u3001\u86cb\u767d\u5076\u806f\u670d\u52d9\u3001\u86cb\u767d\u8868\u9054\u3001\u751f\u5316\/\u7d30\u80de\u5b78\u5be6\u9a57\u4ee3\u6e2c\u3001Elisa\u5be6\u9a57\u4ee3\u6e2c\u3001\u6297\u9ad4\u88fd\u5099\u7b49\uff0c\u6191\u85c9\u5c08\u696d\u6280\u8853\u3001\u8a2d\u5099\u548c\u670d\u52d9\u614b\u5ea6\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u4e00\u7ad9\u5f0f\u5be6\u9a57\u89e3\u6c7a\u65b9\u6848\u3002Abbkine\u6642\u523b\u628a\u63e1\u5ba2\u6236\u9700\u6c42\u8b8a\u5316\uff0c\u4e26\u7a4d\u6975\u61c9\u5c0d\u3001\u53ca\u6642\u8abf\u6574\uff0c\u4f7f\u5f97Abbkine\u6210\u70ba\u8a31\u591a\u570b\u969b\u9818\u5148\u7684\u7814\u7a76\u6a5f\u69cb\u548c\u81e8\u5e8a\u8a3a\u65b7\u516c\u53f8\u503c\u5f97\u4fe1\u8cf4\u7684\u53ef\u9760\u4f9b\u61c9\u5546\u3002<\/p>\n

    BRAND\/AGENCY\/\u53f0\u7063\u4ee3\u7406\u5546<\/span>\/\u00a0<\/span>Abbkine\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/span><\/strong><\/h2>\n

    \"abbkine\u5546\u54c1\u63d2\u5716\"\"\u539f\u5ee0\u9023\u7d5001\"<\/a><\/p>\n

    \"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

    https:\/\/www.abbkine.com\/<\/p>\n

    Abbkine Scientific Co., Ltd\u7531\u4e00\u7fa4\u5fd7\u540c\u9053\u5408\u7684\u79d1\u5b78\u5bb6\u548c\u884c\u92b7\u5c08\u5bb6\uff0c\u57282012\u5e74\u6210\u7acb\u65bc\u7f8e\u570b\u52a0\u5229\u798f\u5c3c\u4e9e\u5dde\uff0c\u96a8\u8457\u4e9e\u6d32\u5e02\u5834\u7684\u8fc5\u731b\u589e\u9577\uff0cAbbkine\u5c07\u7e3d\u90e8\u642c\u9077\u81f3\u4e2d\u570b\u3002\u7d50\u5408\u4e86Abbkine\u7f8e\u570b\u6280\u8853\u548c\u4e2d\u570b\u88fd\u9020\u5169\u65b9\u9762\u7684\u5de8\u5927\u512a\u52e2\uff0cAbbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\uff0c\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002<\/p>\n

    \u767c\u5c55\u6b77\u7a0b<\/span><\/p>\n

    Abbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002Abbkine\u4ee5\u7279\u8272\u7684\u86cb\u767d\u8cea\u751f\u7522\u8207\u6aa2\u6e2c\u8a66\u5291\u8d77\u5bb6\uff0c\u81f4\u529b\u65bc\u5275\u65b0\u548c\u7814\u767c\u5404\u985e\u6297\u9ad4\u3001\u751f\u5316\u8a66\u5291\u3001\u86cb\u767d\u8cea\u548c\u5be6\u9a57\u8a66\u5291\u76d2\uff0c\u4ee5\u671f\u671b\u70ba\u5168\u7403\u79d1\u7814\u5de5\u4f5c\u8005\u63d0\u4f9b\u9769\u65b0\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6211\u5011\u5f88\u81ea\u8c6a\u5730\u70ba\u5ba2\u6236\u63d0\u4f9b\u5982\u4e0b\u7279\u8272\u7522\u54c1\u7dda\uff0c\u4e26\u6703\u4e00\u5982\u65e2\u5f80\u5730\u958b\u767c\u65b0\u578b\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u751f\u547d\u79d1\u5b78\u7814\u7a76\u548c\u8a3a\u65b7\u8a66\u5291\u7522\u54c1\u30022017\u5e74\u6aa2\u6e2c\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u7684ELISA\u8a66\u5291\u76d2\u61c9\u904b\u800c\u751f: \u6839\u64da\u5e02\u5834\u9700\u6c42\uff0c\u958b\u767cEliKine\u2122\u7cfb\u5217ELISA\u8a66\u5291\u76d2\uff0c\u6db5\u84cb\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u71b1\u9580\u6307\u6a19\uff0c\u5982\u767d\u4ecb\u7d20\uff08IL\uff09\u3001\u816b\u7624\u58de\u6b7b\u56e0\u6578\uff08TNF\uff09\u3001\u5e72\u64fe\u7d20\uff08IFN\uff09\u7b49\u660e\u661f\u7d1a\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\u5168\u7403\u767c\u552e\u30022018\u5e74\u6191\u85c9\u5148\u9032\u7684\u86cb\u767d\u8cea\u5076\u806f\u6280\u8853\u548c\u56b4\u683c\u7684\u54c1\u8cea\u63a7\u5236\uff0c\u6211\u5011\u63d0\u4f9bLinKine\u2122\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\uff0c\u5728\u6297\u9ad4\u3001\u86cb\u767d\u8cea\u3001\u591a\u80bd\u6216\u8005\u5176\u5b83\u751f\u7269\u5206\u5b50\u4e0a\u53ef\u76f4\u63a5\u6a19\u8a18HRP\uff0cBiotin\uff0cFITC\uff0cCy3\uff0cAbFluor\u2122\u4ee5\u6eff\u8db3\u79d1\u7814\u61c9\u7528\uff0c\u85e5\u7269\u7814\u767c\u4ee5\u53ca\u8a3a\u65b7\u8a66\u5291\u76d2\u958b\u767c\u7684\u9700\u6c42\u30022019\u5e74\u751f\u5316\u3001\u7d30\u80de\u985e\u7522\u54c1\u7dda\u9010\u6b65\u64f4\u5145\u642d\u5efa\u7d30\u80de\u7522\u54c1\u7814\u767c\u5e73\u81fa\uff0c\u7d30\u80de\u72c0\u614b\u6aa2\u6e2c\u3001\u7d30\u80de\u67d3\u8272\u3001\u7d30\u80de\u63d0\u53d6\u3001\u751f\u5316\u8a66\u5291\u76d2\u7522\u54c1\u5f97\u5230\u8d8a\u4f86\u8d8a\u591a\u5168\u7403\u5ba2\u6236\u7684\u8a8d\u53ef\u3002<\/p>\n

    \"\"<\/p>\n

      \n
    1. \u5168\u5957\u8868\u4f4d\u6a19\u7c64\u6297\u9ad4\u548c\u5167\u53c3\u6297\u9ad4\uff0c\u4ee5\u53ca\u76f8\u61c9\u7684\u5b9a\u5236\u5076\u806f\u7522\u54c1<\/li>\n
    2. \u5c0f\u5305\u88dd\u7684\u78f7\u9178\u5316\u548c\u7532\u57fa\u5316\u7279\u7570\u6027\u7684\u6297\u9ad4\u7522\u54c1<\/li>\n
    3. \u6700\u71b1\u9580\u7684\u4e8c\u6297\u7522\u54c1\uff1a\u7528\u65bcIF\u7684IFKine\u7cfb\u5217\u7522\u54c1\uff0c\u4ee5\u53caIP-WB\u7684IPKine\u7cfb\u5217\u7522\u54c1<\/li>\n
    4. \u5168\u5957\u9ad8\u54c1\u8cea\uff0c\u8cb7\u5f97\u8d77\u7684\u86cb\u767d\u8cea\u7d14\u5316\u5de5\u5177\uff08\u586b\u6599\uff0c\u9810\u88dd\u67f1\u548c\u8a66\u5291\u76d2\uff09<\/li>\n
    5. \u66a2\u92b7\u7684\u91cd\u7d44\u86cb\u767d\uff0c\u5305\u542b\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u751f\u9577\u56e0\u6578<\/li>\n
    6. \u66f4\u591a\u7d30\u80de\u529f\u80fd\u5206\u6790\u8a66\u5291\u76d2\uff0cELISA\u8a66\u5291\u76d2\uff0c\u4ee5\u53ca\u7d30\u80de\u793a\u8e64\u8a66\u5291<\/li>\n<\/ol>\n

      \u6b64\u5916\uff0c\u6211\u5011\u9084\u5efa\u7acb\u4e86\u6280\u8853\u670d\u52d9\u5e73\u81fa\uff0c\u5305\u62ec\u514d\u75ab\u5b78\u76f8\u95dc\u5be6\u9a57\u670d\u52d9\uff08WB\/IHC\/IF\/IP\uff09\u3001\u86cb\u767d\u5076\u806f\u670d\u52d9\u3001\u86cb\u767d\u8868\u9054\u3001\u751f\u5316\/\u7d30\u80de\u5b78\u5be6\u9a57\u4ee3\u6e2c\u3001Elisa\u5be6\u9a57\u4ee3\u6e2c\u3001\u6297\u9ad4\u88fd\u5099\u7b49\uff0c\u6191\u85c9\u5c08\u696d\u6280\u8853\u3001\u8a2d\u5099\u548c\u670d\u52d9\u614b\u5ea6\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u4e00\u7ad9\u5f0f\u5be6\u9a57\u89e3\u6c7a\u65b9\u6848\u3002Abbkine\u6642\u523b\u628a\u63e1\u5ba2\u6236\u9700\u6c42\u8b8a\u5316\uff0c\u4e26\u7a4d\u6975\u61c9\u5c0d\u3001\u53ca\u6642\u8abf\u6574\uff0c\u4f7f\u5f97Abbkine\u6210\u70ba\u8a31\u591a\u570b\u969b\u9818\u5148\u7684\u7814\u7a76\u6a5f\u69cb\u548c\u81e8\u5e8a\u8a3a\u65b7\u516c\u53f8\u503c\u5f97\u4fe1\u8cf4\u7684\u53ef\u9760\u4f9b\u61c9\u5546\u3002<\/p>\n

      \u7d30\u80de\u589e\u6b96&\u6bd2\u7406&\u8870\u8001 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
      \u8ca8\u865fKTA1001<\/a><\/span><\/td>\n\u6d3b&\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\n\n

      \u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/span><\/p>\n

      \u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTA3030<\/a><\/span><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTA1020<\/a><\/span><\/td>\nCCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n1000\u500b\u53cd\u61c9<\/span><\/td>\n\u5438\u6536\u5149450nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTA2030<\/a><\/span><\/td>\nEdU Image\u7d30\u80de\u589e\u6b96\u8a66\u5291\u76d2<\/a>\u00a0(BrdU\u66ff\u4ee3\u6cd5)<\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1110<\/a><\/span><\/td>\n\uff08LDH\uff09\u4e73\u9178\u812b\u6c2b\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

      \u03bbmax= 450 nm<\/span><\/p>\n

      1- 20 U\/mL of LDH<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>\u00a0KTA2020<\/a><\/span><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em=535\/615 nm.<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTA1030<\/a><\/span><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      \u00a0OD 565 nm<\/span><\/p>\n

      10,00-100,0000 cells per well<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>BMD00064-50UG<\/a><\/span><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/span><\/td>\n50ug<\/span><\/td>\n\n

      \u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/span><\/p>\n

      \u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u00a0\u7d30\u80de\u51cb\u4ea1&\u7d30\u80de\u67d3\u8272 (8)<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
      \n

      \u8ca8\u865f\u00a0KTA2010<\/a><\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9<\/span><\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTA0002<\/a><\/span><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9<\/span><\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTA4001<\/a><\/span><\/td>\nJC-1<\/u><\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n20\u500b\u53cd\u61c9<\/span><\/td>\n\n

      \uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/span><\/p>\n

      JC-1 Stain<\/span><\/p>\n

      CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/span><\/td>\nDAPI Staining Solution<\/a><\/span><\/td>\n10MG<\/span><\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/span><\/td>\n<\/tr>\n
      \u00a0KTD102-EN<\/a><\/span><\/td>\n\u51cb\u4ea1\u7d30\u80de\u5206\u6790(Annexin V\u6cd5\u548cTUNEL\u6cd5)<\/a><\/span><\/td>\n40\u500b\u5206\u6790\/20\u500b\u5206\u6790<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/span><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em = 500\/520 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/span><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em = 484\/501 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/span><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      DNA Damege\u640d\u50b7\/\u7d30\u80deStress (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1050<\/a><\/span><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 532 nm<\/span><\/p>\n

      1-100 \u00b5M\/L<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1910<\/a><\/span><\/td>\n\u6d3b\u6027\u6c27\uff08ROS\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/span><\/td>\nDCFH-DA (10 mM)<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1400<\/a><\/span><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      colorimetric (540 nm)<\/span><\/p>\n

      \u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1080<\/a><\/span><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/span><\/td>\n<\/tr>\n
      \u00a0\u8ca8\u865f<\/u><\/strong>\u00a0KTB1091<\/a><\/span><\/td>\n\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\u5438\u6536\u5149 520nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1041<\/a><\/span><\/td>\nCheKine\u2122 \u904e\u6c27\u5316\u6c2b (H2O2) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      480\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      OD 580 nm<\/span><\/p>\n

      : 1 \u00b5M-100 \u00b5M<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1210<\/a><\/span><\/td>\nCheKine\u2122 \u8d85\u6c27\u9670\u96e2\u5b50\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      absorption peak at 540nm<\/span><\/p>\n

      0.0125-0.5 mg\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1020<\/a><\/span><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD565 nm<\/span><\/p>\n

      96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u6297\u6c27\u5316\u5206\u6790\u8a66\u5291 (8)<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1030<\/a><\/span><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		200-3.13 U\/ml<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1040<\/a><\/span><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 540 nm<\/span><\/p>\n

      2-75 \u00b5M<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1500<\/a><\/span><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTCA\u5206\u6790<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      480\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      593 nm<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>\u00a0KTB1660<\/a><\/span><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		240nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1600<\/a><\/span><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		412 nm \u00a02-200 \u00b5g\/mL<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB3010<\/a><\/span><\/td>\nCheKine\u2122 \u82b1\u9752\u7d20\u9084\u539f\u9176\uff08ANR\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\nabsorption at 340 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1530<\/a><\/span><\/td>\n\u9ec3\u916e\u985e\u5316\u5408\u7269\u6aa2\u6e2c\u5957\u7d44<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      absorption peak at 502 nm<\/span><\/p>\n

      0.156-10 mg\/g<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1140<\/a><\/span><\/td>\n\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      \u00a0absorption peak at 525 nm<\/span><\/p>\n

      \u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u81bd\u56fa\u9187\u4ee3\u8b1d\/\u4ee3\u8b1d\u8a66\u9a57 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1510<\/a><\/span><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      \u00a0\u5438\u6536\u5149505 nm<\/span><\/p>\n

      Standard: 5 \u03bcmol\/mL.<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1300<\/a><\/span><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      \u00a0\u5438\u5149\u503c630nm<\/span><\/p>\n

      4- 300 mg\/dL)<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1340<\/a><\/span><\/td>\nCheKine\u2122 \u809d\u91a3\/\u7cd6\u539f<\/u><\/strong>\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB2220<\/a><\/span><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      500nm Standard: 5 \u03bcmol\/mL<\/span><\/p>\n

      \u00a0<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB2250<\/a><\/span><\/td>\nCheKine\u2122 \u5fae\u91cf\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (HDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      Absorption peak at 500nm<\/span><\/p>\n

      Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol\/L\uff09<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB2260<\/a><\/span><\/td>\nCheKine\u2122 \u5fae\u91cf\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (LDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		\n

      Absorption peak at 500nm<\/span><\/p>\n

      Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol\/L\uff09<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1390<\/a><\/span><\/td>\nCheKine\u2122 \u6fb1\u7c89\u5206\u652f\u9176 (SBE) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		absorption at 660 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/span><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 450 nm<\/span><\/p>\n

      \u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u751f\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u53cd\u61c9<\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/strong><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1700<\/a><\/span><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n\u5438\u6536\u5149510 nm<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1542<\/a><\/span><\/td>\nTannase(TAN) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

      absorbance at 270 nm<\/span><\/p>\n

      0.3125-20 \u03bcmoL\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1541<\/a><\/span><\/td>\nCheKine\u2122 \u55ae\u5be7\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Tannin Colorimetric Assay Kit<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

      absorbance at 760 nm<\/span><\/p>\n

      0.0156-1 mg\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1290<\/a><\/span><\/td>\nCheKine\u2122 \u70cf\u982d\u9178\u9176 (ACO) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\nabsorption at 340 nm.<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTB1560<\/a><\/span><\/td>\n\u9187\u91af\u57fa\u8f49\u79fb\u9176\uff08AAT\uff09\u6d3b\u6027\u6bd4\u8272\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

      48\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96\u500b\u53cd\u61c9<\/span><\/td>\n

      		absorption at 412 nm.<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/span><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 450 nm<\/span><\/p>\n

      \u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865fKTB1380<\/a><\/span><\/td>\n\u03b2-\u6fb1\u7c89\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

      absorption peak at 540 nm<\/span><\/p>\n

      0.0156-1 mg\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTB1310<\/a><\/span><\/td>\n\u8461\u8404\u7cd6\u6c27\u5316\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 580 \u4e0b\u6e2c\u91cf<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d:\u00a00.05 – 2 U\/L<\/u><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u767c\u708e\u53cd\u61c9ELISA\u5206\u6790<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u53cd\u61c9<\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/strong><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KET7013<\/a><\/span><\/td>\nIL-6 ELISA Kit<\/u><\/strong>\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		\n

      450 nm<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KET6032<\/a><\/span><\/td>\nTNF<\/u><\/em>–\u03b1<\/u><\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		\n

      450 nm<\/span><\/p>\n

      7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KET6033<\/a><\/span><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		\n

      450 nm<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/span><\/td>\n<\/tr>\n

      KET7013<\/span><\/td>\nIL-22 ELISA\u8a66\u5291\u76d2<\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KET7003<\/a><\/span><\/td>\nIFN-\u03b3\uff08\u5e72\u64fe\u7d20-\u03b3\uff09ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		31.25 pg\/ml- 2000 pg\/ml<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKET6019<\/a><\/span><\/td>\nIL-10 ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      \u00a0<\/span><\/td>\n

      		2.35 pg\/ml-150 pg\/ml<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKET7002<\/span><\/td>\nGM-CSF ELISA\u8a66\u5291\u76d2<\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n7.8 pg\/mL-500 pg\/mL<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>KET7005<\/a><\/span><\/td>\n\u767d\u4ecb\u7d20-1\u03b2ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

      96\u500b\u53cd\u61c9\/<\/span><\/p>\n

      96 X5\u76e4<\/span><\/td>\n

      		\n

      Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/span><\/p>\n

      \u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u91cd\u7d44\u86cb\u767d&\u55ae\u591a\u682a\u6297\u9ad4<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>PRP1012<\/a><\/span><\/td>\nHuman IL-6 protein\u91cd\u7d44\u86cb\u767d\u00a0<\/u><\/strong><\/a><\/span><\/td>\n5ug<\/span><\/td>\nhuman IL-6 (NP_000591.1) (Val 30-Met 212)\/ E. coli<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>PRP100273<\/a><\/span><\/td>\nHuman IL-4 protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong><\/a><\/span><\/td>\n20ug<\/span><\/td>\nED50 for this effect is 0.05-0.2 ng\/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 \u00d7 10E4 IU\/\u03bcg.<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>PRP100159<\/a><\/span><\/td>\nHuman EGF protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong><\/a><\/span><\/td>\n100ug<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>PRP1012<\/a><\/span><\/td>\nHuman IL-6 protein\u91cd\u7d44\u86cb\u767d\u00a0<\/u><\/strong><\/a><\/span><\/td>\n5ug<\/span><\/td>\nhuman IL-6 (NP_000591.1) (Val 30-Met 212)\/ E. coli<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>ABP52783<\/a><\/span><\/td>\nGAPDH Polyclonal Antibody<\/a><\/span><\/td>\n30ul<\/span><\/td>\nHuman, Mouse, Rat\u3002\u53ef\u61c9\u7528\u65bcELISA, IHC-P, WB\u5be6\u9a57\u3002<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fA21010<\/span><\/td>\nHRP, Goat Anti-Mouse IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fA21020<\/span><\/td>\nHRP, Goat Anti-Rabbit IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fA23220<\/span><\/td>\nDylight 488, Goat Anti-Rabbit IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u6297\u9ad4\u86cb\u767d\u7d14\u5316&\u5176\u5b83<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u7279\u9ede<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTP2070<\/a><\/span><\/td>\n\u6297\u9ad4\u7d14\u5316\u8a66\u5291\u76d2\uff08\u86cb\u767d A\/G\uff09<\/a><\/span><\/td>\n1ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u00a0\u8ca8\u865fBMR2001<\/a><\/span><\/td>\nHis-Tag Ni-NTA Resin\u6a39\u8102<\/a><\/span><\/td>\n5ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>\u00a0KTD105-EN<\/a><\/span><\/td>\nIP\/Co-IP Toolkit (Agarose)\u00a0<\/a><\/span><\/td>\n20\u500b\u53cd\u61c9<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865fKTD3001<\/a><\/span><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/span><\/td>\n500\u500b\u53cd\u61c9<\/span><\/td>\n\n

      OD 562 nm<\/span><\/p>\n

      \u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/span><\/p>\n

      50 to 1000 \u00b5g\/ml.<\/span><\/td>\n<\/tr>\n

      \u8ca8\u865f<\/u><\/strong>KTP2140<\/a><\/span><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102(\u5957\u7d44)<\/a><\/span><\/td>\n1ml\u00d75<\/span><\/td>\nMore than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/span><\/td>\n<\/tr>\n
      BMD0062<\/span><\/td>\nHoechst 33342<\/span><\/td>\n10MG<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      BMU104-EN<\/span><\/td>\n\u589e\u5f37\u578bAntifade\u5c01\u7247\u5291<\/span><\/td>\n10ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
      \u8ca8\u865f<\/u><\/strong>BMS0050<\/a><\/span><\/td>\n\u6b63\u5e38\u5c71\u7f8a\u8840\u6e05<\/a><\/span><\/td>\n10ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u7d30\u80de\u57f9\u990a\u76f8\u95dc<\/span><\/strong><\/span><\/p>\n\n\n\n\n
      \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u7279\u9ede<\/td>\n<\/tr>\n
      BMU108-EN<\/strong><\/a><\/span><\/td>\nSuperKine\u2122 \u7121\u8840\u6e05\/\u7121\u86cb\u767d\u7d30\u80de\u51b7\u51cd\u57f9\u990a\u57fa<\/a><\/span><\/td>\n100ML\u00a0<\/span><\/td>\n\u6210\u5206\u6e05\u6670\uff0c\u7121\u8840\u6e05\/\u7121\u86cb\u767d\uff0c\u907f\u514d\u75c5\u6bd2\u3001\u7d30\u83cc\u3001\u652f\u539f\u9ad4\u7b49\u6c61\u67d3<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

       <\/p>\n

       <\/p>\n","protected":false},"excerpt":{"rendered":"

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