{"id":27189,"date":"2021-04-09T15:56:19","date_gmt":"2021-04-09T07:56:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27189"},"modified":"2022-07-19T14:31:58","modified_gmt":"2022-07-19T06:31:58","slug":"tev%e8%9b%8b%e7%99%bd%e9%85%b6-ezcut-tev-protease-recombinant%e8%b2%a8%e8%99%9f7847-1000","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27189","title":{"rendered":"TEV\u86cb\u767d\u9176 | EZCut\u2122 TEV Protease, Recombinant\u8ca8\u865f7847-1000"},"content":{"rendered":"

TEV\u86cb\u767d\u9176 | \u00a0\u8ca8\u865f<\/strong>7847-1000<\/a><\/span><\/p>\n

\"biovision01\"<\/p>\n

EZCut\u2122 TEV Protease, Recombinant<\/p>\n

\"\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"BioVision\u7684EZCut\u2122TEV\u86cb\u767d\u9176\u662f\u4e00\u7a2e\u534a\u80f1\u6c28\u9178\u86cb\u767d\u9176(cysteine protease)\uff0c\u53ef\u8b58\u5225Glu-Xaa- Xaa-Y- Xaa-Gln-\uff08Gly \/ Ser\uff09\u7684\u5207\u5272\u4f4d\u9ede\uff0c\u4e26\u5728Gln\u548cGly \/ Ser\u4e4b\u9593\u5207\u5272\u3002 \u6700\u4f73\u5e8f\u5217\u662fGlu-Asn-Leu-Tyr-Phe-Gln-Ser \/\u7518\u6c28\u9178\uff08ENLYFQS \/ G\uff09\u3002 \u5b83\u5305\u542b\u589e\u5f37\u578b\u7684\u7159\u8349\u8755\u523b\u75c5\u6bd2\uff08Tobacco etch virus , TEV\uff09NIa\u86cb\u767d\u50ac\u5316\u7247\u6bb5\u3002 TEV\u86cb\u767d\u9176\u662f\u4e00\u7a2e\u9650\u5236\u6027\u9176\uff0c\u57284C\u6642\u5177\u6709\u5f88\u5f37\u7684\u6d3b\u6027\uff0c\u4e26\u5177\u6709\u5f88\u9ad8\u7684\u7279\u7570\u6027\u548c\u7a69\u5b9a\u6027\u3002 \u7528\u8a72\u9176\u88c2\u89e3\u7684\u6700\u4f73\u6eab\u5ea6\u662f34\u2103\u3002 \u86cb\u767d\u9176\u53ef\u7528\u65bc\u5f9e\u878d\u5408\u86cb\u767d\u4e2d\u53bb\u9664\u89aa\u548c\u6a19\u7c64\u3002 \u5b83\u5305\u542b\u4e00\u500bC\u672b\u7aef\u7684His\u6a19\u7c64\uff0c\u5728\u88c2\u89e3\u53cd\u61c9\u5f8c\uff0c\u901a\u904e\u4f7f\u53cd\u61c9\u901a\u904eNi\u87af\u5408\u6a39\u8102(Ni-chelating resin)\u5373\u53ef\u8f15\u9b06\u9664\u53bb\u3002 BioVision\u7684EZCut\u2122TEV\u86cb\u767d\u9176\u662fTEV\u86cb\u767d\u9176\u7684\u6539\u826f\u7248\uff0c\u5177\u6709\u9ad8\u4f4d\u9ede\u7279\u7570\u6027\uff0c\u9ad8\u6d3b\u6027\uff0c\u4e26\u4e14\u6bd4\u5929\u7136TEV\u86cb\u767d\u9176\u7a69\u5b9a\u5f97\u591a\uff0c\u5f9e\u800c\u589e\u5f37\u4e86\u9577\u671f\u6d3b\u6027\u3002<\/p>\n

\"\"<\/p>\n

\"7847\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/strong><\/td>\nNuclear inclusion protein A, NIa protein<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nE. coli<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLiquid<\/td>\n<\/tr>\n
Physical Form Description<\/strong><\/td>\n1 mg\/ml solution in 0.1 M Tris-HCl, 0.5 M NaCl, 20% glycerol, 5 mM DTT and 0.5 mM EDTA, pH 8.0<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n28.6 kDa (2038\u20132279 aa + C-terminal poly-his tag).<\/td>\n<\/tr>\n
Purity by SDS-PAGE<\/strong><\/td>\n\u226595%<\/td>\n<\/tr>\n
Biological Activity<\/strong><\/td>\nThe EZCut\u2122 TEV Protease has an activity of \u226510 mU\/mg<\/td>\n<\/tr>\n
Amino Acid Sequence<\/strong><\/td>\n2038\u20132279 aa<\/td>\n<\/tr>\n
Handling<\/strong><\/td>\nCentrifuge the vial prior to opening.<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-80\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

\"Spectrumlab<\/a>\"0100-01\"<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

TEV\u86cb\u767d\u9176 | \u00a0\u8ca8\u865f7847-1000 EZCut\u2122 TEV Protease, Recombinant […]<\/p>\n","protected":false},"author":3,"featured_media":18514,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1147,1146],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27189"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27189"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27189\/revisions"}],"predecessor-version":[{"id":30291,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27189\/revisions\/30291"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/18514"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27189"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27189"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27189"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}