{"id":27134,"date":"2021-03-31T11:18:31","date_gmt":"2021-03-31T03:18:31","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27134"},"modified":"2025-07-28T15:28:12","modified_gmt":"2025-07-28T07:28:12","slug":"%e5%ae%9a%e9%87%8f-%e7%b5%84%e7%b9%94%e8%83%bahistamine%e6%aa%a2%e6%b8%ac-histamine-test-system%e8%b2%a8%e8%99%9f61341","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27134","title":{"rendered":"(\u5b9a\u91cf) \u7d44\u7e54\u80fa(Histamine)\u6aa2\u6e2c | Histamine Test system\u8ca8\u865f61341"},"content":{"rendered":"<p><strong><span style=\"font-size: 12pt; color: #ff6600;\"><span style=\"font-size: 14pt;\"><span style=\"color: #800080;\">Kikkoman\u7cfb\u5217\u5546\u54c1-&gt;&gt;<span style=\"color: #ff9900;\">\u7d44\u7e54\u80fa(Histamine)\u6aa2\u6e2c<\/span><\/span><\/span><\/span><\/strong><\/p>\n<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=27134\"><span style=\"color: #33cccc; font-size: 12pt;\">(\u5b9a\u91cf) \u7d44\u7e54\u80fa(Histamine)\u6aa2\u6e2c <strong>| <\/strong>Histamine Test system<strong>\u8ca8\u865f<\/strong><em><strong>61341<\/strong><\/em><\/span><\/a><\/span><\/p>\n<p><strong>\u7522\u54c1\u63cf\u8ff0:<\/strong><\/p>\n<p>Histamine Test system is a rapid colorimetric enzyme assay system for the quantitative analysis of histamine in raw fish, frozen fish or canned tuna. \u7d44\u7e54\u80fa(Histamine)\u6aa2\u6e2c\u7cfb\u7d71(Histamine Test system, \u8ca8\u865f61341)\u00a0 \u662f\u4e00\u7a2e\u5feb\u901f\u5448\u8272\u6aa2\u6e2c\u7cfb\u7d71\uff0c\u7528\u65bc\u5b9a\u91cf\u5206\u6790\u751f\u9b5a\uff0c\u51b7\u51cd\u9b5a\u6216\u91d1\u69cd\u9b5a\u7f50\u982d\u4e2d\u7684\u7d44\u7e54\u80fa\u542b\u91cf\u3002\u53ea\u9700\u6eb6\u89e3\u8a66\u5291\u4e26\u8207\u6a23\u54c1\u6df7\u5408\u5373\u53ef\u3002 \u8a72\u5206\u6790\u65e2\u7c21\u55ae\u53c8\u5b89\u5168\u3002 \u7531\u65bc\u9176\u7684\u9ad8\u7279\u7570\u6027\uff0c\u56e0\u6b64\u4e0d\u9700\u8981\u8af8\u5982\u5f37\u9178\u6216\u6709\u6a5f\u6eb6\u5291\u4e4b\u985e\u7684\u6709\u5bb3\u5316\u5b78\u7269\u8cea\u3002\u53cd\u61c9\u50c5\u970015\u5206\u9418\u3002 \u5c0d\u65bc\u751f\u9b5a\u6a23\u54c1\uff0c\u5f9e\u6a23\u54c1\u88fd\u5099\u5230\u6700\u7d42\u7d50\u679c\uff0c\u4e00\u5c0f\u6642\u5373\u53ef\u5b8c\u5168\u5b8c\u6210\u6e2c\u8a66\u3002AOAC\u6027\u80fd\u6e2c\u8a66\u65b9\u6cd5\uff08PTM\uff09\u8a8d\u8b49\u3002 \u8a72\u6e2c\u8a66\u5df2\u986f\u793a\u8207\u5b98\u65b9\u5be6\u9a57\u5ba4\u53c3\u8003\u65b9\u6cd5\u9ad8\u5ea6\u76f8\u95dc\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/07\/7-%E5%AE%9A%E9%87%8F-%E7%B5%84%E7%B9%94%E8%83%BAHistamine%E6%AA%A2%E6%B8%AC-Histamine-Test-system%E8%B2%A8%E8%99%9F61341.png\" \/><\/p>\n<p>\u30c1\u30a7\u30c3\u30af\u30ab\u30e9\u30fc \u30d2\u30b9\u30bf\u30df\u30f3\u306f\u3001HPLC\u6cd5\u306e\u3088\u3046\u306a\u7169\u96d1\u306a\u64cd\u4f5c\u306f\u4e0d\u8981\u3067\u3001\u3057\u304b\u3082\u77ed\u6642\u9593\u3067\u7cbe\u5ea6\u826f\u304f\u751f\u9b5a\u4e2d\u306e\u30d2\u30b9\u30bf\u30df\u30f3\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u62bd\u51fa\u64cd\u4f5c\u304c\u7c21\u5358\u3067\u3001HPLC\u6cd5\u3084AOAC\u6cd5(\uff0a)\u306b\u6bd4\u3079\u6e2c\u5b9a\u59a8\u5bb3\u7269\u8cea\u3092\u9664\u304f\u305f\u3081\u306e\u524d\u51e6\u7406\u64cd\u4f5c\u304c\u4e0d\u8981\u3067\u3059\u3002<\/p>\n<p>(\uff0a)AOAC\u6cd5\uff1a Official Methods of Analysis of AOAC International \u63b2\u8f09\u306e\u6e2c\u5b9a\u6cd5<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/07\/4-%E5%AE%9A%E9%87%8F-%E7%B5%84%E7%B9%94%E8%83%BAHistamine%E6%AA%A2%E6%B8%AC-Histamine-Test-system%E8%B2%A8%E8%99%9F61341.png\" width=\"995\" height=\"286\" \/><\/p>\n<div class=\"frame_title\">\u7279\u5fb4<\/div>\n<div class=\"feature_h\">\n<dl>\n<dt>\u7c21\u3000\u5358<\/dt>\n<dd>\u8a66\u85ac\u3092\u6eb6\u304b\u3057\u30b5\u30f3\u30d7\u30eb\u3068\u6df7\u305c\u308b\u3060\u3051<br \/>\n\u9175\u7d20\u306e\u9ad8\u3044\u7279\u7570\u6027\u306b\u3088\u308a\u3001\u5f37\u9178\u3084\u6709\u6a5f\u6eb6\u5a92\u3092\u4f7f\u308f\u305a\u306b\u3001\u8ab0\u306b\u3082\u7c21\u5358\u30fb\u5b89\u5168\u306a\u5206\u6790\u304c\u53ef\u80fd\u3067\u3059\u3002<\/dd>\n<dt>\u8fc5\u3000\u901f<\/dt>\n<dd>\u53cd\u5fdc\u6642\u9593\u306f15\u5206<br \/>\n\u751f\u9b5a\u306a\u3089\u3001\u62bd\u51fa\u4f5c\u696d\u3092\u542b\u3081\u3066\u30821\u6642\u9593\u4ee5\u5185\u306b\u5206\u6790\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3059\u3002<\/dd>\n<dt>\u6b63\u3000\u78ba<\/dt>\n<dd>\u56fd\u969b\u8a8d\u8a3c\u6a5f\u95a2AOAC-RI\u306ePTM\u8a8d\u8a3c\u3092\u53d6\u5f97<br \/>\n\u516c\u5b9a\u6cd5\u3068\u9ad8\u3044\u76f8\u95a2\u304c\u3042\u308a\u307e\u3059\u3002<\/dd>\n<\/dl>\n<\/div>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/07\/5-%E5%AE%9A%E9%87%8F-%E7%B5%84%E7%B9%94%E8%83%BAHistamine%E6%AA%A2%E6%B8%AC-Histamine-Test-system%E8%B2%A8%E8%99%9F61341.png\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/07\/6%E5%AE%9A%E9%87%8F-%E7%B5%84%E7%B9%94%E8%83%BAHistamine%E6%AA%A2%E6%B8%AC-Histamine-Test-system%E8%B2%A8%E8%99%9F61341.png\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u7d44\u7e54\u80fa\u5b9a\u91cf\u5957\u7d44 \u7c21\u6613\u64cd\u4f5c\u6d41\u7a0b<\/strong><\/span><\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/tZ0FAcs2Ml0\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><br \/>\n<span style=\"font-size: 12pt; color: #0000ff;\">\u6a23\u54c1\u8655\u7406<\/span><br \/>\n1. \u79e4 10 g\u9b5a\u7d44\u7e54\uff0c\u5c07\u5176\u5747\u8cea\u5f8c\uff0c\u53d6 1 g\u5230 50 mL\u96e2\u5fc3\u7ba1\u4e2d\u3002<br \/>\n2. \u52a0\u5165 24 mL\u6a23\u54c1 \u8655\u7406\u6db2 (0.1M EDTA\u00b72Na(pH8.0)\u6eb6\u6db2\u6216 0.5M EDTA(pH8.0)\u6eb6\u6db2 \u7a00\u91cb\u4e94\u500d )\uff0c\u6df7\u548c\u5747\u52fb\u3002<br \/>\n3. \u716e\u6cb8 20\u5206\u9418\u3002<br \/>\n4. \u7f6e\u65bc\u51b0\u4e0a\uff0c\u4f7f\u5176\u964d\u6eab\u81f3 20 \u4ee5\u4e0b\u3002<br \/>\n5. \u7528\u4e7e\u6de8\u7684\u522e\u5200\u5c07\u6a23\u54c1\u5145\u5206\u61f8\u6d6e\uff0c\u518d\u6b21\u51b0\u6d74\u51b7\u537b\uff0c\u4f7f \u56fa \u76f8 (\u5305\u62ec\u8102\u80aa )\u548c\u6db2<br \/>\n\u76f8\u5206\u96e2\u3002<br \/>\n6. \u5c07\u6db2\u76f8 \u7528\u6298\u758a\u6ffe\u7d19 (5C)\u5c07\u5167\u5bb9\u7269\u904e\u6ffe\u5230 \u4e7e \u6de8\u7684 \u5bb9\u5668 \u4e2d \u6216 \u8005 \u96e2\u5fc3<br \/>\n(10,000 x g 5\u5206\u9418 )\u5f8c \u6536\u96c6\u4e0a\u6e05\u6db2 \uff0c\u662f\u70ba\u6a23\u54c1\u8403\u53d6\u6db2\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/07\/3-%E5%AE%9A%E9%87%8F-%E7%B5%84%E7%B9%94%E8%83%BAHistamine%E6%AA%A2%E6%B8%AC-Histamine-Test-system%E8%B2%A8%E8%99%9F61341.png\" width=\"643\" height=\"425\" \/><\/p>\n<p><span style=\"color: #0000ff; font-size: 12pt;\">\u8a66\u5291\u6e96\u5099<\/span><br \/>\n\u2022 Colorimetric reagent<br \/>\n1. \u53d6\u4e00\u74f6 Colorimetric reagent\uff0c\u52a0\u5165 11 mL\u84b8\u993e\u6c34\uff0c\u8f15\u8f15\u6416\u6643\u81f3\u5b8c\u5168<br \/>\n\u6eb6\u89e3\u3002<br \/>\n2. \u907f\u5149 !<br \/>\n3. \u7f6e\u65bc\u51b0\u4e0a (0 &#8211; 10\u2103\u2103)\u3002<br \/>\n\u2022 Enzyme reagent<br \/>\n1. \u5c0f\u5fc3\u6253\u958b\u74f6\u84cb\uff0c\u52a0\u5165 6 mL Buffer(\u7c89\u8272\u6a19\u7c64 \u8f15\u8f15\u6416\u6643\u81f3\u5b8c\u5168\u6eb6<br \/>\n\u89e3 \u3002<br \/>\n2. \u7f6e\u65bc\u51b0\u4e0a (0 &#8211; 10\u2103\u2103)\u3002<\/p>\n<p>\u6e2c\u8a66\u6b65\u9a5f (\u53ef\u540c\u6642\u9032\u884c\u4e26 \u5168\u7a0b\u907f\u5149 )(\u53ef\u5c0d\u7167\u4e0b\u65b9\u8868\u683c )<br \/>\n\u2022 Es (Sample)<br \/>\n1. \u53d6 0.5 mL\u6a23\u54c1 \u8403\u53d6 \u6db2\u3002<br \/>\n2. \u52a0\u5165 0.5 mL Colorimetric reagent\u3002<br \/>\n3. \u52a0\u5165 0.5 mL Enzyme reagent\u3002<br \/>\n4. \u6df7\u5408\u5747\u52fb\uff0c\u7f6e\u65bc 37\u2103\u4e0b 15\u5206\u9418\u3002<br \/>\n5. \u6e2c\u91cf\u5438\u5149\u503c (470 nm)(\u7528\u84b8\u993e\u6c34\u4f5c\u70ba Blank)\u3002<br \/>\n6. \u5982\u679c\u5438\u5149\u503c\u5927\u65bc 1\uff0c\u7528\u84b8\u993e\u6c34\u7a00\u91cb \u6a23\u54c1\u8655\u7406\u6db2\uff0c\u91cd\u8907\u6b65\u9a5f 1 ~ 5\u3002<\/p>\n<p>Eb (Sample blank)<br \/>\n1. \u53d6 0.5 mL\u6a23\u54c1 \u8403\u53d6 \u6db2\u3002<br \/>\n2. \u52a0\u5165 0.5 mL Colorimetric reagent\u3002<br \/>\n3. \u52a0\u5165 0.5 mL Buffer(\u7c89\u8272\u6a19\u7c64 )\u3002<\/p>\n<p>Estd (\u6a19\u6e96\u6db2 )<br \/>\n1. \u53d6 0.5 mL\u7d44\u7e54\u80fa\u6a19\u6e96\u6db2 (\u85cd\u8272\u6a19\u7c64 )\u3002<br \/>\n2. \u52a0\u5165 0.5 mL Colorimetric reagent\u3002<br \/>\n3. \u52a0\u5165 0.5 mL Enzyme reagent\u3002<br \/>\n4. \u6df7\u5408\u5747\u52fb\uff0c\u7f6e\u65bc 37\u2103\u4e0b 15\u5206\u9418\u3002<br \/>\n5. \u6e2c\u91cf\u5438\u5149\u503c (470 nm) (\u7528\u84b8\u993e\u6c34\u4f5c\u70ba Blank)\u3002<br \/>\n6. \u5438\u5149\u503c\u5927\u7d04\u662f 0.5(\u5149\u5f91\u9577 10 mm)\u3002<\/p>\n<p>Ec (Reagent blank)<br \/>\n1. \u53d6 0.5 mL\u84b8\u993e\u6c34 \u3002<br \/>\n2. \u52a0\u5165 0.5 mL Colorimetric reagent\u3002<br \/>\n3. \u52a0\u5165 0.5 mL Buffer(\u7c89\u8272\u6a19\u7c64 )\u3002<br \/>\n4. \u6df7\u5408\u5747\u52fb\uff0c\u7f6e\u65bc 37\u2103\u4e0b 15\u5206\u9418\u3002<br \/>\n5. \u6e2c\u91cf\u5438\u5149\u503c (470 nm) (\u7528\u84b8\u993e\u6c34\u4f5c\u70ba Blank)\u3002<br \/>\n6. \u5438\u5149\u503c \u6703\u5c0f\u65bc 0.05(\u5149\u5f91\u9577 10 mm)\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/07\/2-%E5%AE%9A%E9%87%8F-%E7%B5%84%E7%B9%94%E8%83%BAHistamine%E6%AA%A2%E6%B8%AC-Histamine-Test-system%E8%B2%A8%E8%99%9F61341.png\" width=\"721\" height=\"322\" \/><\/p>\n<p>\u7d50\u679c\u5206\u6790<br \/>\n\u7d44\u7e54\u80fa\u6fc3\u5ea6<br \/>\n(mg\/L = ppm)<br \/>\n= (Es \u2013 Eb) \u00f7 (Estd \u2013 Ec) \u00d7 4 \u00d7 25 \u00d7 df<br \/>\n= (Es \u2013 Eb) \u00f7 (Estd \u2013 Ec) \u00d7 100 x df<br \/>\ndf : Es\u8655\u7406\u4e2d\uff0c\u5982\u679c\u6c92\u6709\u7a00\u91cb\uff0c df = 1<br \/>\n4 :\u7d44\u7e54\u80fa\u6a19\u6e96\u6db2\u4e2d\uff0c\u7d44\u7e54\u80fa\u6fc3\u5ea6\u70ba 4 ppm\u3002<br \/>\n25 :\u6a23\u54c1 \u8655\u7406\u6642\uff0c \u6a23\u54c1 \u7a00\u91cb\u4e86 25\u500d\u3002<\/p>\n<table width=\"800\">\n<tbody>\n<tr>\n<td>Number of measurements<\/td>\n<td>60 assays<\/td>\n<\/tr>\n<tr>\n<td>Composition<\/td>\n<td>\n<p>Enzyme reagent (lyophilisate)x 6<\/p>\n<p>Colorimetric reagent (lyophilisate) x 6<\/p>\n<p>Histamine Standard solution x 1<\/p>\n<p>Buffer x 3<\/td>\n<\/tr>\n<tr>\n<td>Storage<\/td>\n<td>2-8 \u2103<\/td>\n<\/tr>\n<tr>\n<td>Expiry<\/td>\n<td>End of month after 31 months from manufacturing date.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?p=9187\">\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/a>\u00a0|\u00a0 <a href=\"http:\/\/biopioneer.com.tw\/?p=16198\">Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0| \u00a0<a href=\"http:\/\/biopioneer.com.tw\/?p=17586\">\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/a>\u00a0|\u00a0 <a href=\"http:\/\/biopioneer.com.tw\/?p=19075\">Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0 <a href=\"http:\/\/biopioneer.com.tw\/?p=1120\">ELISA\u5100\u5668<\/a><\/p>\n<p>&nbsp;<\/p>\n<p><iframe loading=\"lazy\" title=\"\u30c1\u30a7\u30c3\u30af\u30ab\u30e9\u30fc\u30d2\u30b9\u30bf\u30df\u30f3\uff08AOAC-RI\u306ePTM\u8a8d\u8a3c\u53d6\u5f97\uff09\u306e\u64cd\u4f5c\u52d5\u753b\" src=\"\/\/www.youtube.com\/embed\/recqV_ua1i8\" width=\"433\" height=\"260\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Kikkoman\u7cfb\u5217\u5546\u54c1-&gt;&gt;\u7d44\u7e54\u80fa(Histamine)\u6aa2\u6e2c (\u5b9a\u91cf) \u7d44\u7e54\u80fa(Histamin [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":27136,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[708],"tags":[1153],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27134"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27134"}],"version-history":[{"count":8,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27134\/revisions"}],"predecessor-version":[{"id":52116,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27134\/revisions\/52116"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/27136"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27134"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27134"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}