{"id":27023,"date":"2021-03-12T15:25:59","date_gmt":"2021-03-12T07:25:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27023"},"modified":"2025-11-17T17:21:36","modified_gmt":"2025-11-17T09:21:36","slug":"%e8%83%b0%e8%9b%8b%e7%99%bd%e9%85%b6%e5%a4%a7%e8%b1%86%e5%9f%b9%e9%a4%8a%e5%9f%ba-tryptic-soy-broth-tsb%ef%bc%89-%e8%b2%a8%e8%99%9fncm0004a","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27023","title":{"rendered":"TSB\u80f0\u86cb\u767d\u9176\u5927\u8c46\u57f9\u990a\u57fa | Tryptic Soy Broth, TSB\u8ca8\u865fNCM0004A"},"content":{"rendered":"

\u98df\u54c1\u5fae\u751f\u7269\u4e4b\u6aa2\u9a57\u65b9\u6cd5\uff0d\u5316\u599d\u54c1\u4e2d\u7684\u7d30\u83cc\u6c61\u67d3\u7269\/\u6aa2\u6e2c\u6d3b\u75ab\u82d7\u4e2d\u7684\u6d3b\u83cc<\/strong><\/span><\/p>\n

TSB\u80f0\u86cb\u767d\u9176\u5927\u8c46\u57f9\u990a\u57fa (Tryptic Soy Broth, TSB\uff09| \u8ca8\u865fNCM0004A<\/a><\/span>\u00a0 \u00a0\u542bAgar\u8ca8\u865fNCM0002A\u00a0\u00a0<\/a><\/span><\/span><\/p>\n

\"NEOGEN\u5546\u54c1\u5716\"<\/p>\n

\u7b26\u5408\u7f8e\u570b\u85e5\u5178\uff08USP\uff09\/\u7f8e\u570b\u85e5\u5178\uff08EP\uff09\/\u65e5\u672c\u85e5\u5178\uff08JP\uff09\u7684\u5354\u8abf\u8981\u6c42<\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0TSB\u80f0\u86cb\u767d\u9176\u5927\u8c46\u57f9\u990a\u57fa\uff08\u5927\u8c46\u916a\u86cb\u767d\u6d88\u5316\u57f9\u990a\u57faTryptic Soy Broth, TSB\uff09\u7528\u65bc\u57f9\u990a\u591a\u7a2e\u5fae\u751f\u7269\u3002\u80f0\u86cb\u767d\u9176\u5927\u8c46\u57f9\u990a\u57fa\u7b26\u5408\u7f8e\u570b\u85e5\u5178\uff08USP\uff09\/\u7f8e\u570b\u85e5\u5178\uff08EP\uff09\/\u65e5\u672c\u85e5\u5178\uff08JP\uff09\u7684\u5354\u8abf\u8981\u6c42\uff0c\u4e0d\u9069\u7528\u65bc\u4eba\u985e\u75be\u75c5\u6216\u5176\u4ed6\u75be\u75c5\u7684\u8a3a\u65b7\u3002\u80f0\u86cb\u767d\u9176\u5927\u8c46\u57f9\u990a\u57fa\uff08\u5927\u8c46\u916a\u86cb\u767d\u6d88\u5316\u57f9\u990a\u57faTryptic Soy Broth, TSB\uff09\u70ba\u4e00\u7a2e\u901a\u7528\u57f9\u990a\u57fa\uff0c\u901a\u5e38\u7a31\u70ba\u916a\u86cb\u767d\u5927\u8c46(Casein Soya Bean)\u6216\u5927\u8c46\u916a\u86cb\u767d\u6d88\u5316\u57f9\u990a\u57fa(Soybean-Casein Digest broth)\uff0c\u7e2e\u5beb\u70baTSB\u3002\u8a72\u57f9\u990a\u57fa\u6700\u521d\u88ab\u958b\u767c\u7528\u65bc\u4e0d\u4f7f\u7528\u8840\u6db2\u4f86\u6e2c\u5b9a\u78fa\u80fa\u985e\u6297\u80ba\u708e\u93c8\u7403\u83cc (sulfonamides against pneumococci)\u548c\u5176\u4ed6\u751f\u7269\u7684\u6709\u6548\u6027\u3002\u5728\u53ad\u6c27\u689d\u4ef6(anaerobic conditions)\u4e0b\u57f9\u990a\u6642\uff0c\u68ad\u72c0\u82bd\u80de\u687f\u83cc\u548c\u975e\u5b62\u5b50\u578b\u53ad\u6c27\u83cc\u5728\u8a72\u57f9\u990a\u57fa\u4e2d\u5927\u91cf\u751f\u9577\u3002\u5efa\u8b70\u4f7f\u7528TSB\u6e2c\u8a66\u5316\u599d\u54c1\u4e2d\u7684\u7d30\u83cc\u6c61\u67d3\u7269\uff0c\u4e26\u7b26\u5408\u98df\u54c1\u884c\u696d\u7684\u65e2\u5b9a\u6a19\u6e96\u3002\u7f8e\u570b\u8fb2\u696d\u90e8\u52d5\u690d\u7269\u885b\u751f\u6aa2\u9a57\u5c40\u9078\u64c7TSB\u4f86\u6aa2\u6e2c\u6d3b\u75ab\u82d7\u4e2d\u7684\u6d3b\u83cc\u3002 TSB\u7b26\u5408\u7f8e\u570b\u85e5\u5178\uff08USP\uff09\uff0c\u6b50\u6d32\u85e5\u5178\uff08EU\uff09\u548c\u65e5\u672c\u85e5\u5178\uff08JP\uff09\u7684\u5354\u8abf\u898f\u5b9a\u3002 TSB\u8c50\u5bcc\u7684\u71df\u990a\u57fa\u790e\uff0c\u518d\u52a0\u4e0aSPS\u548cCO2\uff0c\u662f\u8840\u6db2\u57f9\u990a\u7684\u6975\u4f73\u57f9\u990a\u57fa\u3002\u6dfb\u52a06.5\uff05NaCl\u5f8c\uff0cTSB\u53ef\u7528\u65bcD\u7d44\u93c8\u7403\u83cc\u7684\u9078\u64c7\u6027\u751f\u9577\u3002\u916a\u86cb\u767d\u7684\u9176\u6d88\u5316\u548c\u5927\u8c46\u7c89\u7684\u9176\u6d88\u5316\u662fTSB\u4e2d\u7684\u6c2e\u6e90\u3002\u8461\u8404\u7cd6\u662f\u4fc3\u9032\u751f\u7269\u751f\u9577\u7684\u78b3\u80fd\u6e90\u3002\u6c2f\u5316\u9209\u4fdd\u6301\u6ef2\u900f\u5e73\u8861\uff1b\u78f7\u9178\u6c2b\u4e8c\u9240\u662f\u4e00\u7a2e\u7de9\u6c96\u5291\u3002<\/p>\n

\"LAB011\"<\/p>\n\n\n\n\n\n\n\n\n
Grams per Liter<\/td>\n30.00<\/td>\n<\/tr>\n
Liters per 500g<\/td>\n17.00<\/td>\n<\/tr>\n
Mass<\/td>\n500 g<\/td>\n<\/tr>\n
Package Dimensions<\/td>\n4.60 IN x 2.90 IN x 8.60 IN<\/td>\n<\/tr>\n
Package Weight<\/td>\n1.35 LB<\/td>\n<\/tr>\n
Preparation<\/td>\n\n
    \n
  1. Dissolve 30 g of the medium in one liter of purified water.<\/li>\n
  2. Mix thoroughly.<\/li>\n
  3. Autoclave at 121\u00b0C for 15 minutes.<\/li>\n<\/ol>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    6<\/strong>\u516c\u5347\u8ff7\u4f60\u57f9\u990a\u7bb1<\/strong><\/a> | \u83cc\u6db2\u6e2c\u91cf<\/strong><\/a>\u00a0<\/strong>(<\/strong>\u5206\u5149\u5149\u5ea6\u8a08<\/strong>)<\/strong>\u00a0<\/strong>\u00a0|\u00a0<\/u>\u6ec5\u83cc\u91dc<\/strong><\/a>\u00a0 |\u00a0<\/strong>\u6c34\u6d74\u69fd<\/strong>WaterBath<\/strong><\/em><\/a>\u00a0|\u00a0<\/strong>\u96fb\u5b50\u5929\u5e73<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>pH<\/strong>\u9178\u9e7c\u5ea6\u8a08<\/strong><\/a>\u00a0|\u00a0<\/strong>\u00a0Vortex\u00a0<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>Pipet aid\u00a0<\/strong><\/a>\u00a0|\u00a0<\/strong>Petri dishes <\/strong>\u57f9\u990a\u76bf<\/strong><\/a><\/span><\/p>\n

    \u57f9\u990a\u57fa\u4f7f\u7528\u65b9\u6cd5<\/strong>: <\/strong>\u5c0730\u514b\u57f9\u990a\u57fa\u6eb6\u65bc1\u5347\u7d14\u6de8\u6c34\u4e2d\u3002\u5982\u6709\u5fc5\u8981\uff0c\u7d93\u5e38\u652a\u62cc\u52a0\u71b1\u4ee5\u5b8c\u5168\u6eb6\u89e3Medium\u3002\u5728121\u2103\u4e0b\u9ad8\u58d3\u6ec5\u83cc15\u5206\u9418\u3002\u7b49\u51b7\u53bb\u5f8c\uff0c\u6700\u7d42pH\uff1a7.3\u00b10.2, 25\u2103<\/p>\n

    \u6aa2\u9a57\u65b9\u6cd5\uff1a\u6aa2\u9ad4\u7d93\u7cfb\uf99c\u7a00\u91cb\u5f8c\uff0c\u4ee5\u5e73\u677f\u8a08\uf969\u57f9\u990a\u57fa\u57f9\u990a\u53ca\u8a08\uf969\u4e4b\u65b9\u6cd5\u3002 2.1. \u5de5\u4f5c\u74b0\u5883\uff1a\u5de5\u4f5c\u5e73\u53f0\u9808\u5bec\u655e\u3001\u6f54\u6de8\u3001\u5149\u7dda\uf97c\u597d\uff0c\u64cd\u4f5c\u5e73\u53f0\u5149\ufa01\u70ba 100 \u544e\u71ed\u5149\u4ee5\u4e0a\uff0c\u5bc6\u9589\u5ba4\u5167\u63db\u6c23\uf97c\u597d\uff0c\u5118\u53ef\u80fd\u6c92\u6709\u7070\u5875\u53ca\uf9ca \u52d5\u7a7a\u6c23\u3002\u6bcf 15 \u5206\u9418\uf918\u83cc\uf969\uf967\u5f97\u8d85\u904e 15 CFU\/\u57f9\u990a\u76bf\u3002\u66f4\u591a\u8cc7\u6599\u4f86\u81eaFDA\u751f\u83cc\u6578\u4e4b\u6aa2\u9a57 (\u53c3\u8003\u65b9\u6cd5) <\/a><\/p>\n\n\n\n\n\n\n\n\n
    Formula<\/strong><\/td>\nLiter<\/strong><\/td>\n<\/tr>\n
    Enzymatic Digest of Casein<\/td>\n17.0 g\/L<\/td>\n<\/tr>\n
    Enzymatic Digest of Soybean<\/td>\n3.0 g\/L<\/td>\n<\/tr>\n
    Sodium Chloride<\/td>\n5.0 g\/L<\/td>\n<\/tr>\n
    Dipotassium Hydrogen Phosphate<\/td>\n2.5 g\/L<\/td>\n<\/tr>\n
    Glucose Monohydrate<\/td>\n2.5 g\/L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u66f4\u591a\u76f8\u95dc\u5546\u54c1 \u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>| \u74ca\u8102\u81a0\u9ad4\u7c89\u672b\uff08\u5206\u751f\u5be6\u9a57\u7b49\u7d1a\uff09<\/a> |\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | \u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> |Mupid 2 DNA\u6c34\u6e96\u96fb\u6cf3\u69fd<\/a>| 3M\u5fae\u751f\u7269\u5feb\u901f\u6aa2\u6e2c\u7247<\/a><\/p>\n

    \"\"<\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u98df\u54c1\u5fae\u751f\u7269\u4e4b\u6aa2\u9a57\u65b9\u6cd5\uff0d\u5316\u599d\u54c1\u4e2d\u7684\u7d30\u83cc\u6c61\u67d3\u7269\/\u6aa2\u6e2c\u6d3b\u75ab\u82d7\u4e2d\u7684\u6d3b\u83cc TSB\u80f0\u86cb\u767d\u9176\u5927\u8c46\u57f9\u990a\u57fa (Tryptic S […]<\/p>\n","protected":false},"author":3,"featured_media":24358,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1184,1185],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27023"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27023"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27023\/revisions"}],"predecessor-version":[{"id":40427,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27023\/revisions\/40427"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24358"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27023"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27023"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27023"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}