{"id":27008,"date":"2021-03-10T12:06:26","date_gmt":"2021-03-10T04:06:26","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27008"},"modified":"2025-11-11T11:35:24","modified_gmt":"2025-11-11T03:35:24","slug":"lentivirus%e7%97%85%e6%af%92%e4%b8%80%e5%80%8b%e6%ad%a5%e9%a9%9fqpcr%e5%ae%9a%e9%87%8f%e5%a5%97%e7%b5%84-qpcr-lentivirus-titer-kit%e8%b2%a8%e8%99%9flv900","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=27008","title":{"rendered":"Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44 | qPCR Lentivirus Titer Kit\u8ca8\u865fLV900"},"content":{"rendered":"

Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44 | qPCR Lentivirus Titer Kit\u8ca8\u865fLV900<\/span><\/strong><\/span><\/a><\/p>\n

\u5e02\u9762\u4e0a\u552f\u4e00\u5b8c\u5168\u6d88\u9664 NTC \u7684\u5957\u4ef6
\nabm \u7684 qPCR \u6162\u75c5\u6bd2\u6ef4\u5ea6\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u4e00\u6b65\u6e2c\u5b9a\u6cd5\uff0c\u63a1\u7528\u5feb\u901f RNA \u8403\u53d6\u6b65\u9a5f\uff0c\u7136\u5f8c\u9032\u884c RT-qPCR\u3002\u8a72\u8a66\u5291\u76d2\u65e8\u5728\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u548c\u7279\u7570\u6027\uff0c\u8207\u5e02\u9762\u4e0a\u7684\u985e\u4f3c\u8a66\u5291\u76d2\u76f8\u6bd4\uff0c\u53ef\u78ba\u4fdd\u6700\u5c0f\u7684\u975e\u7279\u7570\u6027\u80cc\u666f\u548c\u66f4\u597d\u7684\u6574\u9ad4\u6027\u80fd\u3002 ROX \u53c3\u8003\u67d3\u6599\u8207 MasterMix \u5206\u958b\u63d0\u4f9b\uff0c\u4f7f\u5f97\u8a72\u5957\u4ef6\u8207\u5927\u591a\u6578 qPCR \u5100\u5668\u666e\u904d\u76f8\u5bb9\u3002\u5957\u4ef6\u7279\u9ede\uff1a\u7bc0\u7701\u6642\u9593\u4e26\u6d88\u9664\u4e0d\u6e96\u78ba\u6027\uff1a\u5feb\u901f RNA \u8403\u53d6’\u7121 NTC \u64f4\u589e\uff1as\u7368\u7279\u7684\u5f15\u5b50\u8a2d\u8a08\uff0c\u6d88\u9664\u4e86\u975e\u7279\u7570\u6027 (NTC) \u8a0a\u865f\u3002\u53ef\u76f4\u63a5\u7528\u65bc\u7d14\u75c5\u6bd2\u9846\u7c92\u3001\u6e1b\u5c11\u8b8a\u7570\u6027\uff1a\u5373\u7528\u578b\u8a66\u5291\u6df7\u5408\u7269\u3001\u4e00\u6b65 RT-qPCR\uff1a\u6bd4\u5176\u4ed6\u65b9\u6cd5\u66f4\u9748\u654f\u3001\u66f4\u6e96\u78ba\u3001\u542b\u6709\u8207 SYBR Green\u2122 \u548c EvaGreen\u2122 \u76f8\u7576\u7684\u67d3\u6599<\/p>\n

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Lentivirus<\/strong>\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44 (\u5b9a\u91cf)\u00a0 |\u8ca8\u865fLV900<\/strong><\/a><\/span><\/p>\n

Abmgood\u63d0\u4f9b\u5feb\u901f\u4e14\u65b9\u4fbf\u4e4bLentivirus\u75c5\u6bd2\u529b\u50f9\u5b9a\u91cf\u5957\u7d44 (abm<\/strong>\u2019s\u00a0qPCR Lentivirus Titer Kit, LV900);<\/strong>\u4e0d\u9700\u8981\u984d\u5916\u7684\u88c2\u89e3\u6216\u9006\u8f49\u9304\uff08reverse transcription , RT\uff09\u6b65\u9a5f\uff0c\u53ea\u9700\u52a0\u5165\u75c5\u6bd2\u6db2 (\u7d14\u5316\u5f8c\u6216\u75c5\u6bd2\u4e0a\u6e05\u6db2) \u4e26\u4e14\u53ef\u4ee5\u5728\u4e00\u5c0f\u6642\u5167\u5b8c\u6210Lentivirus\u75c5\u6bd2\u529b\u50f9\u5b9a\u91cf\u3002Abm\u7684BlasTaq\u2122DNA\u805a\u5408\u9176\u5177\u6709\u5148\u5929\u7684RT\u6d3b\u6027\uff0c\u56e0\u6b64\u7121\u9700\u6dfb\u52a0RTase\u6216\u984d\u5916\u7684RT\u5b75\u80b2\u6b65\u9a5f\u3002\u8207\u5e02\u5834\u4e0a\u7684\u540c\u985e\u8a66\u5291\u76d2\u76f8\u6bd4\uff0c\u8a72\u8a66\u5291\u76d2\u65e8\u5728\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u548c\u7279\u7570\u6027\uff0c\u53ef\u78ba\u4fdd\u6700\u5c0f\u7684\u975e\u7279\u7570\u6027\u80cc\u666f\u548c\u66f4\u597d\u7684\u6574\u9ad4\u6027\u80fd\u30022021\u5e74\u5f8c\u5305\u88dd\u63d0\u4f9bqPCR MasterMix + ROX\uff0c\u4ee5\u652f\u6301\u5404\u7a2eqPCR\u6a5f\u5668\u7684\u4f7f\u7528\u3002(\u820a\u5305\u88dd\u9700\u55ae\u7368\u8cfc\u8cb72X qPCR MasterMix)\u3002LV900\u75c5\u6bd2\u529b\u50f9\u8a08\u7b97EXCEL\u8868<\/a><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
Product Component<\/strong><\/th>\nQuantity<\/strong><\/th>\n<\/tr>\n<\/thead>\n
BlasTaq\u2122 2X qPCR Titer MasterMix<\/td>\n1.25 ml<\/td>\n<\/tr>\n
Primer Mix<\/td>\n100 rxn (200 \u00b5l)<\/td>\n<\/tr>\n
Standard Control DNA<\/td>\n50 \u00b5l<\/td>\n<\/tr>\n
Virus Lysis Buffer<\/td>\n800 \u00b5l<\/td>\n<\/tr>\n
ROX Reference Dye<\/td>\n15 \u00b5l<\/td>\n<\/tr>\n
Nuclease-Free H2<\/sub>O<\/td>\n2 x 1.0 ml<\/td>\n<\/tr>\n<\/tfoot>\n<\/table>\n

<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n

\"\"\"\"<\/p>\n

    \n
  1. One-Step qPCR\uff0c1\u5c0f\u6642\u5167\u53ef\u5b8c\u6210\u5b9a\u91cf\u3002<\/li>\n
  2. Primer\u7d93\u7279\u6b8a\u8a2d\u8a08\uff0c\u89e3\u6c7aprimer dimer\u5e72\u64fe\u3002<\/li>\n
  3. \u7c21\u5316\u6d41\u7a0b\u3002\u4e0d\u9700\u5c0d\u75c5\u6bd2\u88c2\u89e3\u7de9\u885d\u6db2\u4ee5\u53caRT\u5b75\u80b2\u6b65\u9a5f\u3002<\/li>\n
  4. \u65b0\u5305\u88dd\u63d0\u4f9bqPCR MasterMix + ROX\uff0c\u4ee5\u652f\u6301\u5404\u7a2eqPCR\u6a5f\u5668\u7684\u4f7f\u7528\u3002(\u820a\u5305\u88dd\u9700\u55ae\u7368\u8cfc\u8cb72X qPCR MasterMix)\u3002<\/li>\n
  5. \u9644\u6709\u4e00\u500b\u6a19\u6e96\u5c0d\u7167DNA\u3002<\/li>\n<\/ol>\n

    LV900\u75c5\u6bd2\u529b\u50f9\u8a08\u7b97EXCEL\u8868<\/a><\/strong><\/span><\/p>\n

    \"\"<\/p>\n

    qPCR\u6162\u75c5\u6bd2\u6ef4\u5ea6\u8a66\u5291\u76d2\u96a8\u9644\u4e00\u500b\u55ae\u7368\u7684ROX\u53c3\u8003\u67d3\u6599\u5c0f\u74f6\uff0c\u53ef\u6839\u64daqPCR\u6a5f\u5668\u985e\u578b\u6dfb\u52a0\uff0c\u5982\u4e0b\u8868\u6240\u793aqPCR Lentivirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below<\/p>\n\n\n\n\n\n\n\n
    MACHINE TYPE<\/th>\nMACHINE<\/th>\n<\/tr>\n<\/thead>\n
    High ROX Machines<\/td>\n\n
      \n
    • ABI\u00a0\u00ae 7000, 7300, 7700, 7900, 7900HT, StepOnePlus\u2122, StepOne\u2122, OpenArray, PRISM\u2122 Sequencing Detection Series<\/li>\n
    • Biometra TOptical<\/li>\n
    • Fluidigm BioMark\u2122<\/li>\n
    • Wafergene SmartChip System<\/li>\n
    • TianLong TL998 System<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
    Low ROX Machines<\/td>\n\n
      \n
    • ABI\u00ae 7500, 7500 Fast, Viia\u2122 7, QuantStudio, QuantStudio 3\/5\/6\/7<\/li>\n
    • BioGene InSyte\u2122<\/li>\n
    • Illumina Eco<\/li>\n
    • Analytikjena qTower Series<\/li>\n
    • Stratagene\u00ae Mx3000, Mx3005, Mx4000<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
    No ROX machines<\/td>\n\n