{"id":26883,"date":"2021-02-01T14:18:26","date_gmt":"2021-02-01T06:18:26","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26883"},"modified":"2025-12-22T10:46:36","modified_gmt":"2025-12-22T02:46:36","slug":"%e7%b4%b0%e8%83%9e%e9%80%b1%e6%9c%9f%e6%9f%93%e8%89%b2%e6%aa%a2%e6%b8%ac-cell-cycle-staining-kit%e8%b2%a8%e8%99%9f-kta2020","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26883","title":{"rendered":"\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c | Cell Cycle Staining Kit\u8ca8\u865f KTA2020"},"content":{"rendered":"

\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c | <\/strong>Cell Cycle Staining Kit\u8ca8\u865f<\/strong> KTA2020<\/a><\/span><\/p>\n

\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

 <\/p>\n

\u7d30\u80de\u9031\u671f\u67d3\u8272 (Cell Cycle) \u7814\u7a76\u7684\u610f\u7fa9<\/span><\/span><\/p>\n

\u7d30\u80deDNA\u542b\u91cf\u7684\u6e2c\u91cf\uff0c\u662f\u78ba\u5b9a\u7d30\u80de\u9031\u671f\u968e\u6bb5(cell cycle)\uff08G1\uff0cS\u548c\/\u6216G2 \/ M\uff09\u7684\u6700\u5e38\u898b\u65b9\u6cd5\u3002\u662f\u57fa\u65bc\u96a8\u8457\u7d30\u80de\u5728\u7d30\u80de\u9031\u671f\u4e2d\uff0cG1\u671f\u7684\u7d30\u80de\u5177\u6709\u4e00\u7d44\u6210\u5c0d\u7684\u67d3\u8272\u9ad4\uff0c\u4e26\u4e14\u5728DNA\u542b\u91cf\u65b9\u9762\u662f\u4e00\u81f4\u7684\u3002 \u53e6\u4e00\u65b9\u9762\uff0cDNA\u7684\u6578\u91cf\u5728S\u671f\u958b\u59cb\u589e\u52a0\u4e00\u500d\uff0c\u56e0\u6b64DNA\u7684\u6578\u91cf\u662fG1\u6578\u91cf\u7684\u4e00\u5230\u5169\u500d\u3002 \u8207G1\u4e2d\u7684\u7d30\u80de\u548c\u5169\u7d44\u6210\u5c0d\u7684\u67d3\u8272\u9ad4\u76f8\u6bd4\uff0cG2 \/ M\u671f\u7684\u7d30\u80de\u5177\u6709\u7684DNA\u91cf\u662f\u5176\u5169\u500d\u3002Abbkine\u7d30\u80de\u9031\u671f\u67d3\u8272\u8a66\u5291\u76d2(Cell Cycle Staining Kit, KTA2020) \u5229\u7528\u4e00\u7a2e\u6838\u67d3\u6599(nuclear dye)\uff0c\u8a72\u6838\u67d3\u6599\u8207\u7d30\u80de\u4e2d\u7684\u6838\u9178\u7d50\u5408\u6703\u7522\u751f\u87a2\u5149\u4fe1\u865f(fluorescence signal)\uff0c\u8a72\u4fe1\u865f\u8207\u7d30\u80deDNA\u542b\u91cf(DNA content) \u6210\u6b63\u6bd4\uff0c\u53ef\u65b9\u4fbf\uff0c\u6e96\u78ba\u5730\u78ba\u5b9a\u7d30\u80de\u5404\u76f8\u4e2d\u7d30\u80de\u9031\u671f(cell cycle)\u7684\u767e\u5206\u6bd4\u3002<\/p>\n

 <\/p>\n

<\/p>\n

<\/p>\n

<\/p>\n

Figure 5.<\/b>\u00a0Flow cytometry analyzed MCF\u20137 cell cycle proportions. Representative cell cycle distribution histograms showing apoptosis in MCF\u20137 cells treated for 72 h with lead compounds such as\u00a0A<\/b>\u00a0(5b<\/b>) and\u00a0B<\/b>\u00a0(3a<\/b>) at concentrations of 5 and 10 mM, and analysis of the number of cells at each cell cycle stage with all phases.<\/p>\n

<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCell Cycle Staining Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nAbbkine Cell Cycle Staining Kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content, providing a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Nuclear Dye (50\u00d7)
\n\u2022 Assay Buffer (10\u00d7)
\n\u2022 RNase A (100\u00d7)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Flow cytometric analysis of cell cycle progression.
\n\u2022 Ready-to-use reagents for staining cells.
\n\u2022 The appropriate observe channel is Ex\/Em=535\/615 nm.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\nThe optimal concentration of the Nuclear Dye and incubation time varies depending on the specific application. The staining conditions may need modified according to the particular cell type.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nThe most common approach to determining the cell cycle stage (G1, S, and\/or G2\/M) is based on measurement of cellular DNA content. As cells progress through the cell cycle, cells in the G1 phase have one set of paired chromosomes and are uniform with respect to DNA content. On the other hand, the amount of DNA begins to double during S phase, so that the amount of DNA is between one and two times the amount in G1. Cells in G2\/M phase have double the amount of DNA compared to cells in G1 and two sets of paired chromosomes.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u64cd\u4f5c\u65b9\u6cd5\"<\/strong><\/p>\n

(\u6a19\u6e96\u6d41\u7a0b\u898b\u539f\u5ee0\u7db2\u7ad9)<\/strong><\/p>\n

1.\u8655\u7406\u7d30\u80de\u4f9d\u64cd\u4f5c\u7528\u624b\u518a\u5efa\u8b70\u65b9\u6cd5\u3002
\n2.\u5c0d\u65bc\u975e\u8cbc\u58c1\u7d30\u80de\uff0c\u901a\u904e\u96e2\u5fc3\uff084oC\uff0c600g\uff0c5min\uff09\u6536\u96c61-2\u00d7106<\/sup>\u500b\u7d30\u80de\u3002\u5c0d\u65bc\u8cbc\u58c1\u7d30\u80de\uff0c\u9996\u5148\u7528\u80f0\u86cb\u767d\u9176(Trypsin)\u6d88\u5316\u7d30\u80de\uff0c\u7136\u5f8c\u96e2\u5fc3\u3002
\n3.\u7528\u51b0\u51b7\u7684PBS\u6d17\u6ecc\u7d30\u80de\u6c89\u6fb1\u5169\u6b21\u3002
\n4.\u5c07\u7d30\u80de600g\u96e2\u5fc35\u5206\u9418\uff0c\u7136\u5f8c\u5c07\u8a66\u7ba1\u8f49\u79fb\u81f3\u51b0\u4e2d\u3002
\n5.\u7528\u51b0\u51b7\u768470\uff05\u4e59\u9187(70% ethanol)\u5728\u84b8\u993e\u6c34\u4e2d\u7de9\u6162\u91cd\u61f8\u7d30\u80de\u3002\u5c07\u7d30\u80de\u653e\u5728-20\u00b0C\u6301\u7e8c2\u5c0f\u6642\u6216\u66f4\u9577\u6642\u9593\uff08\u5efa\u8b70\u9694\u591c\u56fa\u5b9a12-24\u5c0f\u6642\uff09\u3002\u5728\u67d3\u8272\u548c\u5206\u6790\u4e4b\u524d\uff0c\u7d30\u80de\u53ef\u4ee5\u5728-20\u00b0C\u4e0b\u4fdd\u5b58\u6578\u9031\u3002<\/p>\n

\u6ce8\u610f\uff1a\u5c07\u7d30\u80de\u56fa\u5b9a\u5728\u55ae\u500b\u7d30\u80de\u61f8\u6db2\u4e2d\u975e\u5e38\u91cd\u8981\u3002\u7d30\u80de\u56fa\u5b9a\u6642\u5bb9\u6613\u7d50\u584a\u3002\u975e\u5e38\u7de9\u6162\u5730\u9010\u6ef4\u6dfb\u52a0\u521d\u59cb\u9ad4\u7a4d\u768470\uff05\u4e59\u9187\u540c\u6642\u8f15\u8f15\u6e26\u65cb\u6709\u52a9\u65bc\u9632\u6b62\u7d30\u80de\u7d50\u584a\u3002<\/p>\n

6.\u57284\u00b0C\u4e0b\u4ee51000g\u96e2\u5fc35\u5206\u9418\u3002
\n7.\u9664\u53bb\u4e59\u9187\uff0c\u4e26\u5c07\u7d30\u80de\u91cd\u61f8\u65bc1 mL\u51b0\u51b7\u7684PBS\u4e2d\u3002
\n8.\u57284\u00b0C\u4e0b\u4ee5500g\u5c07\u7d30\u80de\u96e2\u5fc310\u5206\u9418\uff0c\u7136\u5f8c\u9664\u53bbPBS\u3002\u91cd\u8907\u6b65\u9a5f7\u548c8\u5169\u6b21\u5fb9\u5e95\u9664\u53bb\u4e59\u9187\u3002
\n9.\u5c07\u7d30\u80de\u91cd\u61f8\u65bc0.5 mL\u67d3\u8272\u6eb6\u6db2\u4e2d\uff0c\u4e26\u65bc37\u00b0C\u5b75\u80b230\u5206\u9418 (\u5728\u9ed1\u6697)\u3002
\n10.\u7528PBS\u6d17\u6ecc\u7d30\u80de\u5169\u6b21\uff0c\u4e26\u7528\u51b0\u51b7\u7684PBS\u91cd\u61f8\u3002\u5206\u6790\u5176\u4e2d\u7684\u7d30\u80de\uff08Ex \/ Em = 535\/615 nm\uff09\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6aa2\u6e2c (24\u5c0f\u6642\u5167)\u3002<\/p>\n

\"\u6ce8\u610f\u4e8b\u9805\"<\/p>\n

\u6838\u67d3\u6599\u7684\u6700\u4f73\u6fc3\u5ea6\u548c\u53cd\u61c9\u6642\u9593\uff0c\u4f9d\u4e0d\u540c\u5be6\u9a57\u61c9\u7528\u6216\u7d30\u80de\u985e\u578b\u9700\u518d\u6700\u4f73\u5316\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
CAT#<\/strong><\/td>\nProduct name<\/strong><\/td>\nclassification<\/strong><\/td>\n<\/tr>\n
KTB1600<\/a><\/td>\nCheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1610<\/a><\/td>\nCheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1620<\/a><\/td>\nCheKine\u2122 Glutathione Reductases (GR) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1630<\/a><\/td>\nCheKine\u2122 Glutathione S-Transferase (GST) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1640<\/a><\/td>\nCheKine\u2122 Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1650<\/a><\/td>\nCheKine\u2122 Thioredoxin Reductase (TrxR) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1660<\/a><\/td>\nCheKine\u2122 Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u512a\u60e0\u52a0\u8cfc\"<\/a><\/span><\/p>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>| Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>| \u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/span><\/p>\n

 <\/p>\n

\"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\n\u54c1\u9805<\/td>\n\u53cd\u61c9<\/td>\n\u6e2c\u91cf\u5100\u5668<\/td>\n<\/tr>\n
\u00a0\u8ca8\u865fKTA1001<\/a><\/td>\n\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\n

\u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/p>\n

\u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/td>\n<\/tr>\n

\n

\u8ca8\u865f\u00a0KTA2010<\/a><\/p>\n

 <\/td>\n

\n

TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/p>\n

 <\/td>\n

50\u500b\u53cd\u61c9<\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/td>\n<\/tr>\n
\n

\u8ca8\u865f\u00a0KTC011001<\/a><\/p>\n

 <\/td>\n

CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/a><\/td>\n500\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx\/Em = 500\/520 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em = 484\/501 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n10MG\/100MG<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTA3030<\/a><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTA1020<\/a><\/td>\nCCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n1000\u500b\u53cd\u61c9\/10,000\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTA2030<\/a><\/td>\n\n

\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/p>\n

(BrdU\u66ff\u4ee3\u6cd5)<\/td>\n

100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/a><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (540 nm)<\/p>\n

\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/td>\n<\/tr>\n

\u8ca8\u865fKTD3001<\/a><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/td>\n\n

2000\u500b\u53cd\u61c9\/<\/p>\n

5000\u500b\u53cd\u61c9<\/td>\n

\n

OD 562 nm<\/p>\n

\u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/p>\n

50 to 1000 \u00b5g\/ml.<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1110<\/a><\/td>\n\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u03bbmax= 450 nm<\/p>\n

1- 20 U\/mL of LDH<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>PRP100153<\/a><\/td>\nIGF-I\u91cd\u7d44\u86cb\u767d<\/a><\/td>\n200UG\/1MG<\/td>\n\n

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)<\/p>\n

> 95 % as determined by SDS-PAGE<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>\u00a0KTA2020<\/a><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em=535\/615 nm.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>BMC21400<\/a><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u9810\u88dd\u96e2\u5fc3\u67f1<\/a><\/td>\n\n

1ml\u00d75<\/p>\n

3mlx5<\/td>\n

More than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTA1200<\/a><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149370 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1080<\/a><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1090<\/a><\/td>\n\u6e05\u9664\u6c2b\u6c27\u81ea\u7531\u57fa\u80fd\u529b\u6e2c\u5b9a\u8a66\u5291<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149 536nm Hydroxyl radical scavenging rate D% =\uff08ASample – AControl\uff09\u00f7\uff08ABlank – AControl\uff09\u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB2220<\/a><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

500nm Standard: 5 \u03bcmol\/mL<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1510<\/a><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u6536\u5149505 nm<\/p>\n

Standard: 5 \u03bcmol\/mL.<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1300<\/a><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u5149\u503c630nm<\/p>\n

4- 300 mg\/dL)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1100<\/a><\/td>\nLactate\u00a0<\/strong><\/a>\u00a0\u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (450 nm)<\/p>\n

0.03 mM-2 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1700<\/a><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\u5438\u6536\u5149510 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1500<\/a><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTAC\u5206\u6790<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

593 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTA0002<\/a><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n20\u500b\u53cd\u61c9\/50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KET7013<\/a><\/td>\nEliKine\u2122 Rat IL-6 ELISA Kit<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KET0003<\/a><\/td>\n\u96cc\u4e8c\u9187\uff08Estradiol , E2\uff09\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d40 pg\/ml-1500 pg\/ml<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KET6032<\/a><\/td>\nTNF<\/em>–\u03b1<\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KET6033<\/a><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KET7005<\/a><\/td>\n\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTE70003<\/a><\/td>\n\u5c0f\u9f20\u9ec3\u560c\u5464\u812b\u6c2b\u9176\/\u6c27\u5316\u9176\uff08XDH\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 22.5 ng\/L-360 ng\/L, \u6700\u4f4e\u9748\u654f\u5ea61.0 ng\/L\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f\u00a0A02051HRP<\/strong><\/a><\/td>\nHis-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0<\/a><\/td>\n50ul\/200ul<\/td>\nWB (1:5000).<\/td>\n<\/tr>\n
\u8ca8\u865fA02170MGB<\/a><\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5), \u78c1\u73e0<\/a><\/td>\n100ul\/400ul\/2ml<\/td>\nGKPIPNPLLGLDST epitope<\/td>\n<\/tr>\n
\u8ca8\u865fKTA4001<\/a><\/td>\nJC-1<\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n\n

100\u500b\u53cd\u61c9\/<\/p>\n

500\u500b\u53cd\u61c9<\/td>\n

\n

\uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/p>\n

JC-1 Stain<\/p>\n

CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1600<\/a><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

412 nm<\/p>\n

2-200 \u00b5g\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n1MG\/10MG\/100MG<\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTP3001<\/a><\/td>\n\u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2<\/a><\/td>\n\n

50\u500b\u53cd\u61c9\/<\/p>\n

200\u500b\u53cd\u61c9<\/td>\n

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) \u2022 Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>BMD00064-50UG<\/a><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/td>\n50ug\/1mg\/10mg<\/td>\n\n

\u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>\u00a0KTB1660<\/a><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n240nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1020<\/a><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD565 nm<\/p>\n

96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1040<\/a><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 540 nm<\/p>\n

2-75 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/strong>KTB1060<\/a><\/td>\n\u904e\u6c27\u5316\u7269\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 664 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d\u70ba15.6-1000 \u00b5U\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1050<\/a><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 532 nm<\/p>\n

1-100 \u00b5M\/L<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1030<\/a><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n200-3.13 U\/ml<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTA1030<\/a><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0OD 565 nm<\/p>\n

10,00-100,0000 cells per well<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c | Cell Cycle Staining Kit\u8ca8\u865f KTA2020   \u7d30\u80de\u9031 […]<\/p>\n","protected":false},"author":3,"featured_media":26712,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1207],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26883"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26883"}],"version-history":[{"count":9,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26883\/revisions"}],"predecessor-version":[{"id":49481,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26883\/revisions\/49481"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/26712"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26883"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26883"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26883"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}