{"id":26869,"date":"2021-01-26T13:24:42","date_gmt":"2021-01-26T05:24:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26869"},"modified":"2022-07-19T15:06:38","modified_gmt":"2022-07-19T07:06:38","slug":"%e5%87%8b%e4%ba%a1%e7%b4%b0%e8%83%9e%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-apoptotic-cell-isolation-kit%e8%b2%a8%e8%99%9fk258","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26869","title":{"rendered":"\u51cb\u4ea1\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2| Apoptotic Cell Isolation Kit\u8ca8\u865fK258"},"content":{"rendered":"

\u51cb\u4ea1\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2| <\/strong>Apoptotic Cell Isolation Kit\u8ca8\u865f<\/strong>K258<\/a><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0:<\/strong> \u51cb\u4ea1\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2(Apoptotic Cell Isolation Kit, K258)\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u6709\u6548\u7684\u65b9\u6cd5\uff0c\u53ef\u4f7f\u7528\u819c\u806f\u86cb\u767dV \/\u78c1\u73e0(annexin V\/magnetic beads)\uff08MagBeads\uff09\u5f9e\u7d30\u80de\u57f9\u990a\u7269\u6216\u7d44\u7e54\u88fd\u5291\u4e2d\u5206\u96e2\u51cb\u4ea1\u7d30\u80de(apoptotic cells)\u6216\u53bb\u9664\u6b7b\u7d30\u80de(dead cells)\u3002\u819c\u806f\u86cb\u767dV(Annexin V)\u662f\u4e00\u7a2e\u5c0dCa 2+\u4f9d\u8cf4\u6027\u7684\u78f7\u8102\u7d50\u5408\u86cb\u767d(Ca\u00b2\u1da7-dependent phospholipid binding protein)\uff0c\u5c0d\u78f7\u8102\u9170\u7d72\u6c28\u9178\uff08phosphatidylserine , PS\uff09\u5177\u6709\u9ad8\u89aa\u548c\u529b\uff0c\u5b83\u5f9e\u5167\u8cea\u819c\u5c0f\u8449\u91cd\u65b0\u5206\u4f48\u5230\u51cb\u4ea1\u6216\u6b7b\u7d30\u80de\u4e2d\u3002\u4e00\u65e6\u9032\u5165\u7d30\u80de\u8868\u9762\uff0cPS\u5c31\u53ef\u7528\u65bc\u7d50\u5408\u819c\u806f\u86cb\u767dV\u53ca\u5176\u4efb\u4f55\u7d50\u5408\u7269\u3002 Annexin V-\u751f\u7269\u7d20\u8207\u51cb\u4ea1\u7d30\u80de\u7684\u7d50\u5408\uff0c\u7136\u5f8c\u751f\u7269\u7d20\u8207\u93c8\u9ef4\u89aa\u548c\u7d20-MagBeads(streptavidin-MagBeads)\u7684\u7d50\u5408\uff0c\u4f7f\u51cb\u4ea1\u7d30\u80de(apoptotic)\u8207\u6d3b\u7d30\u80de\u5206\u96e2\u3002\u8207MagBeads\u7d50\u5408\u7684\u51cb\u4ea1\u7d30\u80de\u7c98\u9644\u5728\u78c1\u9ad4\u4e0a\uff0c\u800c\u975e\u51cb\u4ea1\u7d30\u80de\u5247\u4fdd\u6301\u61f8\u6d6e\u72c0\u614b\u3002\u7136\u5f8c\u53ef\u4ee5\u5c07\u5206\u96e2\u7684\u51cb\u4ea1\u7d30\u80de\u548c\u5065\u5eb7\u7d30\u80de\u7528\u65bc\u5404\u7a2e\u6e2c\u5b9a\u4e2d\uff0c\u4ee5\u7814\u7a76\u51cb\u4ea1\u6a5f\u5236\u548c\u9014\u5f91\u3002\u8a72\u8a66\u5291\u76d2\u4e5f\u5df2\u6210\u529f\u7528\u65bc\u5f9e\u5065\u5eb7\u7d30\u80de\u57f9\u990a\u7269\u4e2d\u53bb\u9664\u6b7b\u7d30\u80de\u3002<\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK258-30<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n30 isolations<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nMagnetic bead bound apoptotic cells can be separated from normal cells<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nKit provides a simple and efficient means for isolation of apoptotic cells from normal culture using annexin V magnetic beads (MagBeads). The separated apoptotic cells and normal cells can be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from normal culture.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes only ~1 hours
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Annexin V-Biotin
\n\u2022 1X Binding Buffer
\n\u2022 Streptavidin-MagBeads
\n\u2022 Apoptotic Cell Releasing Buffer<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u51cb\u4ea1\u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2| Apoptotic Cell Isolation Kit\u8ca8\u865fK258 \u7522\u54c1\u63cf\u8ff0: \u51cb\u4ea1\u7d30 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1211],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26869"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26869"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26869\/revisions"}],"predecessor-version":[{"id":26870,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26869\/revisions\/26870"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26869"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26869"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26869"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}