{"id":26855,"date":"2021-01-21T14:57:43","date_gmt":"2021-01-21T06:57:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26855"},"modified":"2022-07-19T15:07:10","modified_gmt":"2022-07-19T07:07:10","slug":"human-irisin-elisa%e8%a9%a6%e5%8a%91%e7%9b%92-human-irisin-elisa-kit%e8%b2%a8%e8%99%9fkte62744","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26855","title":{"rendered":"Human Irisin ELISA\u8a66\u5291\u76d2| Human Irisin ELISA Kit\u8ca8\u865fKTE62744"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=26855\">Human Irisin ELISA\u8a66\u5291\u76d2<strong>| <\/strong>Human Irisin ELISA Kit<strong>\u8ca8\u865f<\/strong>KTE62744<\/a>\u00a0(15 pg\/mL-240 pg\/mL)<\/span><\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong>Irisin\u662f\u904b\u52d5\u800c\u7531\u9aa8\u9abc\u808c\u7d30\u80de\u5206\u6ccc\u7684\uff0c\u4e26\u5faa\u74b0\u81f3\u8102\u80aa\u7d44\u7e54\uff0c\u5728\u6b64\u8a98\u5c0e\u5411\u68d5\u8272\u8102\u80aa\u7684\u8f49\u8b8a\u3002\u7531\u65bc\u68d5\u8272\u8102\u80aa\u6703\u5c0e\u81f4\u80fd\u91cf\u6563\u5931\uff0c\u56e0\u6b64\u8a72\u9014\u5f91\u5c0d\u65bc\u65b0\u9673\u4ee3\u8b1d\u548c\u80a5\u80d6\u975e\u5e38\u91cd\u8981\u3002Human Irisin ELISA Kit(\u8ca8\u865fKTE62744)\u4f7f\u7528\u5169\u9ede\u593e\u5fc3ELISA(two-site sandwich ELISA)\u5b9a\u91cf\u6a23\u54c1\u4e2d\u7684Irisin\u3002\u5c0dIrisin\u5177\u6709\u7279\u7570\u6027\u7684\u6297\u9ad4\u5df2\u9810\u5148\u5305\u88ab\u5728\u5fae\u677f\u4e0a\u3002\u5c07\u6a19\u6e96\u54c1\u548c\u6a23\u54c1\u5438\u5230\u5b54\u4e2d\uff0c\u4e26\u4e14\u5b58\u5728\u7684\u4efb\u4f55Irisin\u90fd\u88ab\u56fa\u5b9a\u7684\u6297\u9ad4\u7d50\u5408\u3002\u9664\u53bb\u6240\u6709\u672a\u7d50\u5408\u7684\u7269\u8cea\u5f8c\uff0c\u5c07HRP-\u7db4\u5408\u4eba\u985eIrisin\u6aa2\u6e2c\u6297\u9ad4\u6dfb\u52a0\u5230\u5b54\u4e2d\u3002\u6d17\u6ecc\u9664\u53bb\u4efb\u4f55\u672a\u7d50\u5408\u7684HRP\u8a66\u5291\u5f8c\uff0c\u5c07\u8272\u539f\u6eb6\u6db2\u6dfb\u52a0\u5230\u5b54\u4e2d\uff0c\u4e26\u4e14\u984f\u8272\u8207\u521d\u59cb\u6b65\u9a5f\u4e2d\u7d50\u5408\u7684Irisin\u7684\u91cf\u6210\u6bd4\u4f8b\u3002\u505c\u6b62\u986f\u8272\u4e26\u6e2c\u91cf\u984f\u8272\u5f37\u5ea6\u3002<\/p>\n<ul>\n<li><strong>Product name<\/strong><\/li>\n<\/ul>\n<p>Human Irisin ELISA Kit<\/p>\n<ul>\n<li><strong>Reactivity<\/strong><\/li>\n<\/ul>\n<p>Human<\/p>\n<ul>\n<li><strong>Applications<\/strong><\/li>\n<\/ul>\n<p>ELISA<\/p>\n<ul>\n<li><strong>Application notes<\/strong><\/li>\n<\/ul>\n<p>Human Irisin ELISA Kit employs a two-site sandwich ELISA to quantitate Irisin in samples. An antibody specific for Irisin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Irisin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human Irisin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Irisin bound in the initial step. The color development is stopped and the intensity of the color is measured.<\/p>\n<ul>\n<li><strong>Detection method<\/strong><\/li>\n<\/ul>\n<p>Colorimetric<\/p>\n<ul>\n<li><strong>Sample type<\/strong><\/li>\n<\/ul>\n<p>Cell culture supernatants, Other biological fluids, Plasma, Serum<\/p>\n<ul>\n<li><strong>Assay type<\/strong><\/li>\n<\/ul>\n<p>Sandwich ELISA<\/p>\n<ul>\n<li><strong>Calibration range<\/strong><\/li>\n<\/ul>\n<p>15 pg\/mL-240 pg\/mL<\/p>\n<ul>\n<li><strong>Limit of detection<\/strong><\/li>\n<\/ul>\n<p>1.0 pg\/mL<\/p>\n<ul>\n<li><strong>Usage notes<\/strong><\/li>\n<li>Do not mix components from different kit lots or use reagents beyond the kit expiration date.<br \/>\n\u2022 Allow all reagents to warm to room temperature for at least 30 minutes before opening.<br \/>\n\u2022 Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.<br \/>\n\u2022 Unused wells must be kept desiccated at 4 \u00b0C in the sealed bag provided.<br \/>\n\u2022 Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.<br \/>\n\u2022 It is recommended that all samples and standards be assayed in duplicate or triplicate.<\/li>\n<li><strong>Storage instructions<\/strong><\/li>\n<\/ul>\n<p>The unopened kit should be stored at 2 &#8211; 8\u00b0C. After opening, please store refer to protocols.<\/p>\n<ul>\n<li><strong>Shipping<\/strong><\/li>\n<\/ul>\n<p>Gel pack with blue ice.<\/p>\n<p>&nbsp;<\/p>\n<p>\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805<\/p>\n<ul>\n<li>\u8acb\u52ff\u6df7\u7528\u4e0d\u540c\u8a66\u5291\u76d2\u4e2d\u7684\u7d44\u5206\uff0c\u6216\u5728\u8a66\u5291\u76d2\u5230\u671f\u65e5\u671f\u4e4b\u5f8c\u4f7f\u7528\u8a66\u5291\u3002<\/li>\n<li>\u6253\u958b\u4e4b\u524d\uff0c\u8b93\u6240\u6709\u8a66\u5291\u81f3\u5c11\u52a0\u5ba4\u6eab30\u5206\u9418\u3002<\/li>\n<li>\u7528\u8a66\u5291\u9810\u6e05\u6d17\u79fb\u6db2\u5668\u5438\u982d\uff0c\u70ba\u6bcf\u500b\u6a23\u54c1\uff0c\u6a19\u6e96\u6db2\u548c\u8a66\u5291\u4f7f\u7528\u65b0\u9bae\u7684\u79fb\u6db2\u5668\u5438\u982d\uff0c\u4ee5\u514d\u6c61\u67d3\u3002<\/li>\n<li>\u672a\u4f7f\u7528\u7684\u5b54\u5fc5\u9808\u5728\u63d0\u4f9b\u7684\u5bc6\u5c01\u888b\u4e2d\u65bc4\u00b0C\u4e7e\u71e5\u3002<\/li>\n<li>\u5fb9\u5e95\u6df7\u5408\u5c0d\u65bc\u7d50\u679c\u975e\u5e38\u91cd\u8981\u3002\u5efa\u8b70\u6bcf10\u5206\u9418\u4f7f\u7528\u4f4e\u983b\u632f\u76ea\u5668\u6216\u7a0d\u7a0d\u6416\u52d5\u624b\u3002<\/li>\n<li>\u5efa\u8b70\u6240\u6709\u6a23\u54c1\u548c\u6a19\u6e96\u54c1\u4e00\u5f0f\u5169\u4efd\u6216\u4e00\u5f0f\u4e09\u4efd\u9032\u884c\u6e2c\u5b9a\u3002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Human Irisin ELISA\u8a66\u5291\u76d2| Human Irisin ELISA Kit\u8ca8\u865fKTE62744 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":24540,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1215],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26855"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26855"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26855\/revisions"}],"predecessor-version":[{"id":52944,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26855\/revisions\/52944"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24540"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26855"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26855"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26855"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}