{"id":26709,"date":"2020-12-31T12:00:20","date_gmt":"2020-12-31T04:00:20","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26709"},"modified":"2023-09-07T17:05:22","modified_gmt":"2023-09-07T09:05:22","slug":"purkine%e5%85%a7%e6%af%92%e7%b4%a0%e5%8e%bb%e9%99%a4%e6%a8%b9%e8%84%82-purkine-endotoxin-removal-resin%e8%b2%a8%e8%99%9fktp21400","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26709","title":{"rendered":"PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102 (\u5957\u7d44) |PurKine\u2122 Endotoxin Removal Kit (polymyxin B)\u8ca8\u865fKTP2140"},"content":{"rendered":"<p><span style=\"color: #33cccc;\"><strong><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=26709\"><span style=\"font-size: 12pt;\">PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102(\u5957\u7d44)\u00a0\u00a0| PurKine\u2122 Endotoxin Removal Kit (polymyxin B)\u8ca8\u865fKTP2140<\/span><\/a><span style=\"font-size: 12pt;\">\u00a0\u00a0<\/span><\/strong><\/span><\/p>\n<p><span style=\"color: #999999;\">PurKine\u2122 Endotoxin Removal Kit (polymyxin B)\u5957\u7d44\u5305\u62ec\u9810\u88dd\u96e2\u5fc3Column\u3001buffer (1ml; 1mlx5)\u00a0\u00a0<\/span>\u00a0(45-165 \u00b5m)<\/p>\n<p>2,000,000 EU\/mL to eliminate &gt;99% of endotoxins.<\/p>\n<p><span style=\"color: #800000;\">\u2022\u00a0PurKine\u2122 Endotoxin Removal polymyxin B Packed Column<\/span><br \/>\n<span style=\"color: #800000;\"> \u2022 Regeneration buffer(10X)<\/span><br \/>\n<span style=\"color: #800000;\"> \u2022 Equilibration buffer(10x)<\/span><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/02\/PurKine%E2%84%A2%E5%85%A7%E6%AF%92%E7%B4%A0%E5%8E%BB%E9%99%A4%E6%A8%B9%E8%84%82%E5%A5%97%E7%B5%84-PurKine%E2%84%A2-Endotoxin-Removal-Kit-polymyxin-B%E8%B2%A8%E8%99%9FKTP2140-300x216.jpg\" alt=\"PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102(\u5957\u7d44) PurKine\u2122 Endotoxin Removal Kit (polymyxin B)\u8ca8\u865fKTP2140\" \/><\/p>\n<p>\u5167\u6bd2\u7d20(Endotoxin)\u662f\u4f4d\u65bc\u9769\u862d\u6c0f\u9670\u6027\u83cc\u5916\u819c\u7684\u8102\u591a\u91a3(Endotoxinisthelipopolysaccharide, LPS\uff09\u8907\u5408\u7269\u3002\u55ae\u500b\u5927\u8178\u687f\u83cc(E.coli)\u542b\u6709200\u842c\u500bLPS\u5206\u5b50\uff082-20fg\/\u7d30\u80de\uff09\u3002\u5728\u5be6\u9a57\u904e\u7a0b\u4e2d\uff0c\u5927\u91cf\u7684\u5167\u6bd2\u7d20(endotoxins)\u5f88\u5bb9\u6613\u6c61\u67d3\u8017\u6750(consumables)\u3001\u7de9\u885d\u6db2(buffers)\u3001\u6a23\u54c1(samples)\u548c\u5176\u4ed6\u4e0b\u6e38\u7522\u54c1\u3002\u5728\u9ad4\u5916\uff0c\u5167\u6bd2\u7d20\u6703\u5c0e\u81f4\u7d30\u80de\u57f9\u990a( cell-culture )\u4e2d\u7684\u5404\u7a2e\u554f\u984c\u5728\u9ad4\u5167\uff0c\u5167\u6bd2\u7d20\u53ef\u80fd\u6703\u5f15\u8d77\u5404\u7a2e\u526f\u4f5c\u7528\uff0c\u5305\u62ec\u708e\u75c7\u53cd\u61c9(inflammatory response)\u3001\u5668\u5b98\u8870\u7aed(organ failure)\u6216\u6557\u8840\u6027\u4f11\u514b\u5728\u5bbf\u4e3b\u751f\u7269\u9ad4\u3002 PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u8a66\u5291\u76d2(PurKine\u2122 Endotoxin Removal Kit )\u53ef\u4ee5\u5feb\u901f\u6709\u6548\u5730\u6d88\u9664\u5167\u6bd2\u7d20(endotoxins )\uff0c\u5f9e\u800c\u6d88\u9664\u5167\u6bd2\u7d20\u3002\u901a\u904e\u56fa\u5b9a\u5316\u591a\u7c98\u83cc\u7d20B (immobilized Polymyxin B)\uff0c\u5728\u542b\u6709\u86cb\u767d\u8cea\u6216\u85e5\u7406\u5b78\u91cd\u8981\u6210\u5206\u7684\u6eb6\u6db2\u4e2d&lt;0.1EU\/mL\uff0c\u8a72\u7269\u8cea\u4ee5\u6355\u7372\u5167\u6bd2\u7d20\u548c\u9632\u6b62\u6bd2\u6027\u4f5c\u7528\u800c\u805e\u540d\u3002<\/p>\n<p>PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102(PurKine\u2122 Endotoxin Removal Resin) \u53ef\u6709\u6548\u964d\u4f4e\u86cb\u767d\u8cea\u6a23\u54c1\u4e2d\u7684\u5167\u6bd2\u7d20(Endotoxins)\u6c34\u5e73\u3002\u5167\u6bd2\u7d20(Endotoxins)\uff0c\u4e5f\u7a31\u70ba\u8102\u591a\u91a3(lipopolysaccharides)\u6216LPS\uff0c\u662f\u9769\u862d\u6c0f\u9670\u6027\u7d30\u83cc\uff08Gram-negative bacteria\uff09, \u4f8b\u5982\u5927\u8178\u687f\u83cc(E. coli\uff09\u7684\u7d30\u80de\u819c\u6210\u5206(cell membrane components)\u3002\u5167\u6bd2\u7d20(Endotoxins)\u5f37\u70c8\u5f71\u97ffDNA\u8f49\u67d3\u81f3\u539f\u4ee3\u7d30\u80de(primary cells)\u548c\u654f\u611f\u57f9\u990a\u7d30\u80de\u4e2d\uff0c\u5167\u6bd2\u7d20(Endotoxins)\u6c34\u5e73\u5347\u9ad8\u5c0e\u81f4\u8f49\u67d3\u6548\u7387(transfection efficiencies)\u6025\u5287\u964d\u4f4e\u3002\u6a39\u8102(Resin)\u753190\u03bcm\u4ea4\u806f\u76844\uff05\u74ca\u8102\u7cd6\u73e0(cross-linked 4% agarose)\u7d44\u6210\uff0c\u4e14\u7531\u4fee\u98fe\u7684\u591a\u7c98\u83cc\u7d20B(modified polymyxin B)\u8207\u4e4b\u5076\u806f\u3002\u8a72\u7522\u54c1\u7d44\u5408\u5177\u512a\u5316(optimization)\uff0c\u4ee5\u5be6\u73fe\u6700\u5927\u7684\u86cb\u767d\u8cea\u7522\u91cf(protein yield)\uff0c\u7a69\u5b9a\u6027(stability)\u548c\u6eb6\u89e3\u6027(solubility)\u3002\u6e2c\u8a66\u78ba\u8a8d\uff0c\u81f3\u5c11\u91cd\u8907\u4f7f\u7528\u4e94\u6b21\u5f8c\u3002\u6027\u80fd\u4e0d\u6703\u964d\u4f4e\u3002PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u7cfb\u5217\u7522\u54c1\uff0c\u6709\u63d0\u4f9b\u6a39\u8102(<span style=\"color: #666699;\"><a style=\"color: #666699;\" href=\"http:\/\/biopioneer.com.tw\/?p=26718\">PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102<\/a><\/span>, \u8ca8\u865fBMR2140)\u3001\u9810\u88dd\u96e2\u5fc3\u67f1(<span style=\"color: #666699;\"><a style=\"color: #666699;\" href=\"http:\/\/biopioneer.com.tw\/?p=26723\">PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u9810\u88dd\u96e2\u5fc3\u67f1<\/a><\/span>, BMC2140) \u548c\u8a66\u5291\u76d2(<span style=\"color: #666699;\"><a style=\"color: #666699;\" href=\"http:\/\/biopioneer.com.tw\/?p=26709\">PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102(\u5957\u7d44)\u00a0<\/a>\u00a0<\/span>(polymyxin B), KTP2140) \u4e09\u7a2e\u5546\u54c1\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong><\/span><\/p>\n<p>PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u8a66\u5291\u76d2(PurKine\u2122 Endotoxin Removal Kit (polymyxin B), KTP2140) \u65e8\u5728\u5c07\u86cb\u767d\u8cea\u6a23\u54c1\u4e2d\u7684\u5167\u6bd2\u7d20(Endotoxins)\u6c34\u5e73\u964d\u4f4e\u226599\uff05\uff0c\u540c\u6642\u6700\u5927\u9650\u5ea6\u5730\u63d0\u9ad8\u86cb\u767d\u8cea\u56de\u6536\u7387\u3002\u6a39\u8102(Resin)\u753190\u03bcm\u4ea4\u806f\u76844\uff05\u74ca\u8102\u7cd6\u73e0\u7d44\u6210\uff0c\u4fee\u98fe\u7684\u591a\u7c98\u83cc\u7d20B\uff08PMB\uff09\u5df2\u8207\u4e4b\u5076\u806f\u3002\u6e2c\u8a66\u8b49\u5be6\u5176\u6027\u80fd\u7b49\u65bc\u6216\u8d85\u904e\u5176\u4ed6\u4f9b\u61c9\u5546\u7684\u6d41\u884c\u591a\u7c98\u83cc\u7d20B\uff08PMB\uff09\u6a39\u8102\uff0c\u4e26\u4e14\u81f3\u5c11\u91cd\u8907\u4f7f\u7528\u4e94\u6b21\u5f8c\uff0c\u6027\u80fd\u4e0d\u6703\u964d\u4f4e\u3002\u64cd\u4f5c\u624b\u518a<span style=\"color: #008080;\"><a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/06\/PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102\u5957\u7d44-PurKine\u2122-Endotoxin-Removal-Kit-polymyxin-B\u8ca8\u865fKTP21400.pdf\">PurKine\u2122 Endotoxin Removal Kit (polymyxin B)<\/a><\/span><\/p>\n<p><a href=\" http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/06\/PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102\u5957\u7d44-PurKine\u2122-Endotoxin-Removal-Kit-polymyxin-B\u8ca8\u865fKTP21400.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p>PurKine\u2122 Endotoxin Removal Resin has been designed to reduce endotoxin levels in protein samples by \u226599% while maximizing protein recovery. The Resin consists of 90\u03bcm beads of cross-linked 4% agarose, to which modified polymyxin B (PMB) has been coupled. Tests confirm that performance equals or exceeds popular polymyxin B (PMB) resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.<\/p>\n<ul class=\"product-info\">\n<li><span class=\"info-name\">Formulation<\/span>Liquid solution with 50% slurry in 20% ethanol.<\/li>\n<li><span class=\"info-name\">Kit components<\/span>\u2022 PurKine\u2122 Endotoxin Removal polymyxin B Packed Column<br \/>\n\u2022 Regeneration buffer(10X)<br \/>\n\u2022 Equilibration buffer(10x)<\/li>\n<li><span class=\"info-name\">Features &amp; Benefits<\/span><b>\u2022 High capacity<\/b>\u2014More than 2,000,000 EU\/mL to eliminate &gt;99% of endotoxins.<br \/>\n<b>\u2022 High performance<\/b>\u2014Protein recovery is more than 85%.Reducing final EU concentration to 0.1 EU\/mL<br \/>\n<b>\u2022 Cost-effective<\/b>\u2014No performance decrease after at least five repeated uses of the same batch of resin.<br \/>\n<b>\u2022 Flexible<\/b>\u2014available in multiple formats including bulk resin, spin columns and complete kits.<\/li>\n<li><span class=\"info-name\">Storage instructions<\/span>Stable for one year at 2-8\u00b0C from date of shipment. Do not freeze.<\/li>\n<\/ul>\n<p><span style=\"color: #008080;\"><strong><span style=\"font-size: 12pt;\"><a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=24999\">\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0<a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=15880\">CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2\u00a0<\/a>\u00a0|\u00a0<a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=25057\">LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0<a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=25127\">\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0<a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=25092\">\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/span><\/strong><\/span><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><b>Characteristics of ToxinEraser\u2122 Endotoxin Removal Resin<\/b><\/span><\/p>\n<table border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Size<\/td>\n<td>1 ml in pre-packed column<\/td>\n<\/tr>\n<tr>\n<td>Binding Capacity<\/td>\n<td>Up to 2,000,000 EU\/ml resin<\/td>\n<\/tr>\n<tr>\n<td>Ligand<\/td>\n<td>\u00a0modified polymyxin B (Polymixin B, PMB)<\/td>\n<\/tr>\n<tr>\n<td>pH Stability<\/td>\n<td>pH7-8<\/td>\n<\/tr>\n<tr>\n<td>Support Matrix<\/td>\n<td>4% cross-linked agarose, spherical beads<\/td>\n<\/tr>\n<tr>\n<td>Mean Particle Size<\/td>\n<td>90 \u03bcm<\/td>\n<\/tr>\n<tr>\n<td>Storage<\/td>\n<td>2\u00b0C to 8\u00b0C (Do not freeze.)<\/td>\n<\/tr>\n<tr>\n<td>Formulation<\/td>\n<td>Liquid solution with 50% slurry in 20% ethanol.<\/td>\n<\/tr>\n<tr>\n<td>Kit components<\/td>\n<td>\u2022 PurKine\u2122 Endotoxin Removal polymyxin B Packed Column<br \/>\n\u2022 Regeneration buffer(10X)<br \/>\n\u2022 Equilibration buffer(10x)<\/td>\n<\/tr>\n<tr>\n<td>Features &amp; Benefits<\/td>\n<td><b>\u2022 High capacity<\/b>\u2014More than 2,000,000 EU\/mL to eliminate &gt;99% of endotoxins.<br \/>\n<b>\u2022 High performance<\/b>\u2014Protein recovery is more than 85%.Reducing final EU concentration to 0.1 EU\/mL<br \/>\n<b>\u2022 Cost-effective<\/b>\u2014No performance decrease after at least five repeated uses of the same batch of resin.<br \/>\n<b>\u2022 Flexible<\/b>\u2014available in multiple formats including bulk resin, spin columns and complete kits.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102(\u5957\u7d44)\u00a0\u00a0| PurKine\u2122 Endotoxin Removal Kit ( [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":26712,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1251],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26709"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26709"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26709\/revisions"}],"predecessor-version":[{"id":45711,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26709\/revisions\/45711"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/26712"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26709"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26709"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26709"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}