{"id":26689,"date":"2020-12-28T14:53:12","date_gmt":"2020-12-28T06:53:12","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26689"},"modified":"2022-07-19T15:31:16","modified_gmt":"2022-07-19T07:31:16","slug":"%e5%b0%bf%e9%85%b8%e9%85%b6%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk4230-100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26689","title":{"rendered":"Uricase\u5c3f\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | Biovision\u8ca8\u865fK4230-100"},"content":{"rendered":"

\u5c3f\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K4230-100<\/a><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0:<\/strong> \u5c3f\u9178\u9176\uff08Uricase\uff09\u8207\u56e0\u5b50\u7121\u95dc\u7684\u5c3f\u9178\u7fa5\u5316\u9176(urate hydroxylase)\uff0c\u5c3f\u9178\u6c27\u5316\u9176\uff08urate oxidase , UO\uff09\uff1b EC 1.7.3.3\uff09\u662f\u4e00\u7a2e\u53c3\u8207\u5c3f\u9178\u4ee3\u8b1d(uric acid metabolism)\u7684\u9176\u3002\u5c3f\u9178(Uric acid)\u662f\u560c\u5464\u4ee3\u8b1d(purine metabolism)\u7684\u6700\u7d42\u7522\u7269\uff0c\u8840\u6db2\u4e2d\u9ad8\u6c34\u5e73\u7684\u5c3f\u9178(uric acid)\u6703\u5c0e\u81f4\u75db\u98a8(gout)\u3002\u5c3f\u9178\u9176(Uricase)\u5b58\u5728\u65bc\u591a\u7a2e\u54fa\u4e73\u52d5\u7269\u4e2d\uff0c\u4f46\u4eba\u985e\u537b\u4e0d\u5b58\u5728\u3002\u5b83\u5df2\u88ab\u5ee3\u6cdb\u7528\u65bc\u6e2c\u91cf\u751f\u7269\u6a23\u54c1\u4e2d\u7684\u5c3f\u9178\u6fc3\u5ea6\uff0c\u91cd\u7d44\u5c3f\u9178\u9176\uff08rasburicase\uff09\u5df2\u88ab\u7528\u4f5c\u9810\u9632\u816b\u7624\u6eb6\u89e3\u7d9c\u5408\u5fb5\u7684\u85e5\u7269\u3002 BioVision\u7684\u5c3f\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u7c21\u4fbf\u7684\u65b9\u6cd5\u4f86\u6e2c\u91cf\u591a\u7a2e\u6a23\u54c1\u4e2d\u7684\u5c3f\u9178\u9176\u6d3b\u6027\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u5c3f\u9178\u9176\u6c27\u5316\u5c3f\u9178\u5f62\u6210\u8207\u63a2\u91dd\u53cd\u61c9\u7684\u7522\u7269\uff0c\u5f9e\u800c\u7522\u751f\u87a2\u5149\u4fe1\u865f\uff08Ex \/ Em = 535 \/ 587nm\uff09\u3002\u7522\u751f\u7684\u87a2\u5149\u4fe1\u865f\u8207\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u6d3b\u6027\u5c3f\u9178\u9176\u7684\u91cf\u6210\u6b63\u6bd4\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u9748\u654f\u4e14\u9ad8\u901a\u91cf\u7684\u9069\u61c9\u6027\u6e2c\u5b9a\uff0c\u53ef\u4ee5\u6e2c\u91cf\u4f4e\u81f310 \u00b5U\u7684\u751f\u7269\u6a23\u54c1\u4e2d\u5c3f\u9178\u9176\u7684\u6d3b\u6027\u3002<\/p>\n

\u5546\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf<\/span>\u00a0\u00a0\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100\u8acb\u6309\u6b64\u00a0<\/a><\/span>(\u6a19\u914d405\u3001450\u3001492\u3001630 nm, \u53ef\u64f4\u589e\u81f37\u500b\u6ffe\u7247)<\/p>\n

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\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK734-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method– Fluorometric (535\/587 nm)
\n\u2022 Species reactivity- Mammalian\/Plants
\n\u2022 Applications- The kit can detect as low as 10 \u00b5U of Uricase<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nDetection method– Fluorometric (535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nRapid assessment of Uricase activity in mammalian and plant tissues<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 1 hour
\n\u2022 Fast and convenient
\n\u2022 The assay is sensitive, stable and high-throughput adaptable.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Uricase Assay Buffer
\n\u2022 Uricase Substrate
\n\u2022 Uricase Probe (in DMSO)
\n\u2022 Uricase Enzyme Mix
\n\u2022 H2O2 Standard (0.88 M)
\n\u2022 Uricase Positive Control<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u5c3f\u9178\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK4230-100 \u7522\u54c1\u63cf\u8ff0: \u5c3f\u9178\u9176\uff08Uricase\uff09\u8207\u56e0\u5b50\u7121\u95dc\u7684\u5c3f\u9178\u7fa5\u5316\u9176( […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1254],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26689"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26689"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26689\/revisions"}],"predecessor-version":[{"id":26691,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26689\/revisions\/26691"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26689"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26689"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26689"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}