{"id":26646,"date":"2020-12-25T12:49:17","date_gmt":"2020-12-25T04:49:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26646"},"modified":"2022-07-19T15:32:05","modified_gmt":"2022-07-19T07:32:05","slug":"%e8%9b%8b%e7%99%bd%e5%87%8d%e7%b7%a9%e8%a1%9d%e6%b6%b2-buffered-peptone-water-bpw-iso%e8%b2%a8%e8%99%9f105438","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26646","title":{"rendered":"\u86cb\u767d\u51cd\u7de9\u885d\u6db2 | Buffered Peptone Water (BPW) (ISO)\u8ca8\u865fncm0015"},"content":{"rendered":"

6<\/strong>\u516c\u5347\u8ff7\u4f60\u57f9\u990a\u7bb1<\/strong><\/a> | \u83cc\u6db2\u6e2c\u91cf<\/strong><\/a><\/span>\u00a0<\/strong>(<\/strong>\u5206\u5149\u5149\u5ea6\u8a08<\/strong>)<\/strong>\u00a0<\/strong><\/span>\u00a0|\u00a0<\/u>\u6ec5\u83cc\u91dc<\/strong><\/a>\u00a0 |\u00a0<\/strong>\u6c34\u6d74\u69fd<\/strong>WaterBath<\/strong><\/em><\/a>\u00a0|\u00a0<\/strong>\u96fb\u5b50\u5929\u5e73<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>pH<\/strong>\u9178\u9e7c\u5ea6\u8a08<\/strong><\/a>\u00a0|\u00a0<\/strong>\u00a0Vortex\u00a0<\/strong><\/a>\u00a0|\u00a0\u00a0<\/strong>Pipet aid\u00a0<\/strong><\/a>\u00a0|\u00a0<\/strong>Petri dishes <\/strong>\u57f9\u990a\u76bf<\/strong><\/a><\/span><\/p>\n

\u98df\u54c1\u5fae\u751f\u7269\u4e4b\u6aa2\u9a57\u65b9\u6cd5\uff0d\u6c99\u9580\u6c0f\u83cc\u4e4b\u6aa2\u9a57 (Salmonella spp.)<\/p>\n

\u86cb\u767d\u51cd\u7de9\u885d\u6db2 | Buffered Peptone Water (BPW) (ISO)\u8ca8\u865fncm0015<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong>\u7de9\u885d\u86cb\u767d\u8156\u6c34(Buffered Peptone Water, BPW)\uff08ISO\uff09\u7528\u65bc\u98df\u54c1\u4e2d\u591a\u7a2e\u5fae\u751f\u7269\u7684\u975e\u9078\u64c7\u6027\u9810\u6df7\u7de9\u885d\u6db2\uff0c\u5305\u62ec\u6c99\u9580\u6c0f\u83cc\uff08Salmonella spp\uff09\u548c\u514b\u7f85\u8afe\u687f\u83cc\u5c6c(Cronobacter spp)\u3002\u3002 \u4e0d\u9069\u7528\u65bc\u4eba\u985e\u75be\u75c5\u6216\u5176\u4ed6\u75be\u75c5\u7684\u8a3a\u65b7\u3002\u7de9\u885d\u86cb\u767d\u8156\u6c34(Buffered Peptone Water, BPW)\uff08ISO\uff09\u6839\u64daISO 6579-1\uff1a2017\uff0cISO 22694\uff1a2017\uff0cISO 21528-1\uff1a2017\u548cISO 12950\uff1a2010\u5236\u5b9a\u3002\u9019\u7a2e\u975e\u9078\u64c7\u6027\u9810\u589e\u83cc\u57f9\u990a\u57fa\u65e8\u5728\u5e6b\u52a9\u5c07\u4e9e\u81f4\u6b7b\u7684\u6c99\u9580\u6c0f\u83cc\u6062\u5fa9\u5230\u9078\u64c7\u57f9\u990a\u57fa\u4e4b\u524d\u7684\u72c0\u614b\u3002\u4e0d\u542b\u6291\u5236\u5291\uff0c\u4e26\u5177\u6709\u826f\u597d\u7684\u7de9\u885d\u4f5c\u7528\uff0c\u53ef\u5728\u5b75\u80b2\u671f\u9593\u4fdd\u6301pH 7.0\u3002\u5c0d\u6c99\u9580\u6c0f\u83cc\u7684\u4e9e\u81f4\u6b7b\u6027\u50b7\u5bb3\u767c\u751f\u5728\u8a31\u591a\u98df\u54c1\u52a0\u5de5\u904e\u7a0b\u4e2d\uff0cpre-enrichment\u6b65\u9a5f\u5927\u5927\u63d0\u9ad8\u4e86\u9019\u4e9b\u751f\u7269\u7684\u56de\u6536\u7387\u3002<\/p>\n

    \n
  1. For detection of\u00a0Salmonella<\/em>\u00a0spp. (Water Quality) – Refer to ISO 19250:2010<\/li>\n
  2. For detection and enumeration of Enterobacteriaceae – Refer to ISO 21528-1:2017<\/li>\n
  3. For detection and enumeration and Serotyping of\u00a0Salmonella<\/em>\u00a0– Refer to ISO 6579-1:2017<\/li>\n
  4. For detection\u00a0Cronobacter<\/em>\u00a0spp. – Refer to ISO 22964:2017<\/li>\n
  5. For detection of STEC – Refer to ISO\/TS 13136:2012<\/li>\n<\/ol>\n

    \u57f9\u990a\u57fa\u4f7f\u7528\u65b9\u6cd5<\/strong>: <\/strong>\u5c0720.1\u514b\u57f9\u990a\u57fa\u6eb6\u65bc1\u5347\u7d14\u6de8\u6c34\u4e2d\u3002\u5982\u6709\u5fc5\u8981\uff0c\u7d93\u5e38\u652a\u62cc\u52a0\u71b1\u4ee5\u5b8c\u5168\u6eb6\u89e3Medium\u3002\u5728121\u2103\u4e0b\u9ad8\u58d3\u6ec5\u83cc15\u5206\u9418\u3002\u7b49\u51b7\u53bb\u5f8c\uff0c\u6700\u7d42pH\uff1a7.0\u00b10.2, 25\u2103\u5206\u88dd\u65bcPetri dish\u57f9\u990a\u76bf\u4e2d\uff0c\u6aa2\u9a57\u65b9\u6cd5\uff1a\u6aa2\u9ad4\u7d93\u7cfb\uf99c\u7a00\u91cb\u5f8c\uff0c\u4ee5\u5e73\u677f\u8a08\uf969\u57f9\u990a\u57fa\u57f9\u990a\u53ca\u8a08\uf969\u4e4b\u65b9\u6cd5\u3002 2.1. \u5de5\u4f5c\u74b0\u5883\uff1a\u5de5\u4f5c\u5e73\u53f0\u9808\u5bec\u655e\u3001\u6f54\u6de8\u3001\u5149\u7dda\uf97c\u597d\uff0c\u64cd\u4f5c\u5e73\u53f0\u5149\ufa01\u70ba 100 \u544e\u71ed\u5149\u4ee5\u4e0a\uff0c\u5bc6\u9589\u5ba4\u5167\u63db\u6c23\uf97c\u597d\uff0c\u5118\u53ef\u80fd\u6c92\u6709\u7070\u5875\u53ca\uf9ca \u52d5\u7a7a\u6c23\u3002\u6bcf 15 \u5206\u9418\uf918\u83cc\uf969\uf967\u5f97\u8d85\u904e 15 CFU\/\u57f9\u990a\u76bf\u3002<\/p>\n\n\n\n\n\n\n\n
    Formula<\/strong><\/td>\nLiter<\/strong><\/td>\n<\/tr>\n
    Enzymatic Digest of Casein<\/td>\n10.0 g\/L<\/td>\n<\/tr>\n
    Sodium Chloride<\/td>\n5.0 g\/L<\/td>\n<\/tr>\n
    Disodium Hydrogen Phosphate (anhydrous)<\/td>\n3.6 g\/L<\/td>\n<\/tr>\n
    Potassium Dihydrogen Phosphate<\/td>\n1.5 g\/L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u6587\u737b\u53c3\u8003References<\/span><\/strong><\/span><\/p>\n

    1. ISO 19250:2010 Water quality — Detection of Salmonella spp. <\/span><\/p>\n

    2. ISO 21528-1:2017 Microbiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Part 1: Detection of Enterobacteriaceae <\/span><\/p>\n

    3. ISO 6579-1:2017 Microbiology of the food chain \u2013 Horizontal method for the detection, enumeration and serotyping of Salmonella<\/span><\/p>\n

    4. ISO 22964:2017 Microbiology of the food chain — Horizontal method for the detection of Cronobacter spp. <\/span><\/p>\n

    5. ISO\/TS 13136:2012 Microbiology of food and animal feed \u2014 Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens \u2014 Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups<\/span><\/p>\n

    \u66f4\u591a\u8cc7\u6599\u4f86\u81eaFDA\u751f\u83cc\u6578\u4e4b\u6aa2\u9a57 (\u53c3\u8003\u65b9\u6cd5) <\/a><\/h3>\n

    \u66f4\u591a\u76f8\u95dc\u5546\u54c1 \u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>| \u74ca\u8102\u81a0\u9ad4\u7c89\u672b\uff08\u5206\u751f\u5be6\u9a57\u7b49\u7d1a\uff09<\/a> |\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a> | \u5fae\u751f\u7269\u6297\u751f\u7d20<\/a> | \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a> |Mupid 2 DNA\u6c34\u6e96\u96fb\u6cf3\u69fd<\/a>| 3M\u5fae\u751f\u7269\u5feb\u901f\u6aa2\u6e2c\u7247<\/a><\/span><\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    6\u516c\u5347\u8ff7\u4f60\u57f9\u990a\u7bb1 | \u83cc\u6db2\u6e2c\u91cf\u00a0(\u5206\u5149\u5149\u5ea6\u8a08)\u00a0\u00a0|\u00a0\u6ec5\u83cc\u91dc\u00a0 |\u00a0\u6c34\u6d74\u69fdWaterBath\u00a0|\u00a0\u96fb\u5b50\u5929\u5e73\u00a0| […]<\/p>\n","protected":false},"author":3,"featured_media":24358,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1258],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26646"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26646"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26646\/revisions"}],"predecessor-version":[{"id":32135,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26646\/revisions\/32135"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24358"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26646"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26646"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26646"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}