{"id":26619,"date":"2020-12-18T13:53:55","date_gmt":"2020-12-18T05:53:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26619"},"modified":"2022-07-19T15:38:27","modified_gmt":"2022-07-19T07:38:27","slug":"dr5trail-r2-protein-mouse-recombinant-his-tag-%e8%b2%a8%e8%99%9f50412-m08h","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26619","title":{"rendered":"DR5\/TRAIL R2 Protein, Mouse, Recombinant (His Tag)  | \u8ca8\u865f50412-M08H"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=26619\">DR5\/TRAIL R2 Protein, Mouse, Recombinant (His Tag) \u00a0<strong>| <\/strong><strong>\u8ca8\u865f<\/strong>50412-M08H<\/a><\/span><\/p>\n<p><strong>\u7522\u54c1\u63cf\u8ff0:<\/strong> DR5\/TRAIL R2 Protein, Mouse, Recombinant (His Tag). \u5206\u6ccc\u7684\u91cd\u7d44\u5c0f\u9f20TNFRSF10B\u7531136\u500b\u6c28\u57fa\u9178\u7d44\u6210\uff0c\u8a08\u7b97\u7684\u5206\u5b50\u91cf\u70ba15 kDa\u3002\u7531\u65bc\u7cd6\u57fa\u5316\uff0c\u5728\u9084\u539f\u689d\u4ef6\u4e0b\uff0cSDS-PAGE\u4e2d\u91cd\u7d44\u86cb\u767d\u7684\u8868\u89c0\u5206\u5b50\u91cf\u7d04\u70ba25-35 kDa\u3002<\/p>\n<p><strong>Purity<\/strong><\/p>\n<p>&gt; 95 % as determined by SDS-PAGE<\/p>\n<p><strong>Endotoxin<\/strong><\/p>\n<p>&lt; 1.0 EU per \u03bcg of the protein as determined by the LAL method<\/p>\n<p><strong>Activity<\/strong><\/p>\n<p>Immobilized mouse TNFRSF10B-His at 10 \u03bcg\/ml (100 \u03bcl\/well) can bind biotinylated human TNFSF10 (Cat:10409-HNAE), The EC<sub>50<\/sub>\u00a0of biotinylated human TNFSF10 (Cat:10409-HNAE) is 0.16-0.38 \u03bcg\/ml.<\/p>\n<p><strong>Protein Construction<\/strong><\/p>\n<p>A DNA sequence encoding the extracellular domain of mouse TNFRSF10B (NP_064671.2) (Met 1-Ser 177) was expressed, with a C-terminal polyhistidine tag.<\/p>\n<p><strong>Accession#<\/strong><\/p>\n<p><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/protein\/NP_064671.2\">NP_064671.2<\/a><\/p>\n<p><strong>Expressed Host<\/strong><\/p>\n<p>HEK293 Cells<\/p>\n<p><strong>Species<\/strong><\/p>\n<p>Mouse<\/p>\n<p><strong>Predicted N Terminal<\/strong><\/p>\n<p>Asn 53<\/p>\n<p><strong>Molecule Mass<\/strong><\/p>\n<p>The secreted recombinant mouse TNFRSF10B consists of 136 amino acids and has a calculated molecular mass of 15 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 25-35 kDa in SDS-PAGE under reducing conditions.<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?p=9187\"><strong>\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a><strong>\u00a0|\u00a0 <\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=16198\"><strong>Finnpipette F3 <\/strong><strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a><strong>\u00a0| \u00a0<\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=17586\"><strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a><strong>\u00a0|\u00a0 <\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=19075\"><strong>Genereach<\/strong><strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a><strong>\u00a0 |\u00a0\u00a0 <\/strong><a href=\"http:\/\/biopioneer.com.tw\/?p=1120\"><strong>ELISA<\/strong><strong>\u5100\u5668<\/strong><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>DR5\/TRAIL R2 Protein, Mouse, Recombinant (His Tag) \u00a0| \u8ca8 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":3546,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1267,1271,1272],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26619"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26619"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26619\/revisions"}],"predecessor-version":[{"id":26620,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26619\/revisions\/26620"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/3546"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26619"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26619"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26619"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}