{"id":26580,"date":"2020-12-15T13:43:01","date_gmt":"2020-12-15T05:43:01","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26580"},"modified":"2022-07-19T15:42:17","modified_gmt":"2022-07-19T07:42:17","slug":"ki-67%e5%96%ae%e6%a0%aa%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9fabm40064","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26580","title":{"rendered":"Ki-67\u55ae\u682a\u6297\u9ad4 | \u8ca8\u865fABM40064"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=26580\">Ki-67\u55ae\u682a\u6297\u9ad4 <strong>| <\/strong><strong>\u8ca8\u865f<\/strong>ABM40064<\/a><\/span><\/p>\n<p>\u7d30\u80de\u589e\u6b96(Cell proliferation)\u53ef\u7528\u65bc\u8a55\u4f30\u6b63\u5e38\u7684\u7d30\u80de\u5065\u5eb7\u72c0\u6cc1(normal cell health)\uff0c\u6e2c\u91cf\u5c0d\u6bd2\u6027\u640d\u50b7(toxic insult)\u7684\u53cd\u61c9\uff0c\u6216\u7528\u4f5c\u5e7e\u7a2e\u764c\u75c7\u7684\u9810\u5f8c(prognostic)\u548c\u8a3a\u65b7\u5de5\u5177(diagnostic tool)\u3002\u53ef\u7528\u7684\u6a19\u8a18\u901a\u5e38\u8457\u91cd\u65bcDNA\u6c34\u5e73(DNA levels)\u6216\u5408\u6210(synthesis)\uff0c\u7d30\u80de\u4ee3\u8b1d(cellular metabolism)\u6216\u589e\u6b96\u7279\u7570\u6027\u86cb\u767d(proliferation-specific proteins)\u3002<\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong> Ki-67\u662f\u4e00\u7a2e\u7531\u4ebaMKI67\u57fa\u56e0\u7de8\u78bc\u7684\u86cb\u767d\u8cea\u3002\u8a72\u86cb\u767d\u8207\u7d30\u80de\u7684\u589e\u6b96\u5bc6\u5207\u76f8\u95dc\u3002MKI67\u7de8\u78bc\u6838\u86cb\u767d(nuclear protein)\uff08\u589e\u6b96Ki-67\u7684\u6a19\u8a18\uff09\u8207\u7d30\u80de\u589e\u6b96(cellular proliferation)\u76f8\u95dc\u3002Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abbkine.com\/file\/img\/ABM40064-1.jpg\" alt=\"\" width=\"318\" height=\"215\" \/><\/p>\n<p>Fig.1. IHC-P staining of mouse kidney tissue, diluted at 1:200.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abbkine.com\/file\/img\/ABM40064-2.jpg\" alt=\"\" width=\"443\" height=\"130\" \/><\/p>\n<p>Fig.2. Immunofluorescence analysis of human breast cancer tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4\u00b0C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abbkine.com\/file\/img\/ABM40064-3.jpg\" alt=\"\" width=\"453\" height=\"174\" \/><\/p>\n<p>Fig.3. Immunofluorescence analysis of mouse testis tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4\u00b0C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abbkine.com\/file\/img\/ABM40064-4.jpg\" alt=\"\" width=\"462\" height=\"182\" \/><\/p>\n<p>Fig.4. Immunofluorescence analysis of rat brain tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4\u00b0C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abbkine.com\/file\/img\/ABM40064-5.jpg\" alt=\"\" width=\"345\" height=\"226\" \/><\/p>\n<p>Fig.5. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4\u00b0C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (&gt;98\u00b0C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abbkine.com\/file\/img\/ABM40064-6.jpg\" alt=\"\" width=\"343\" height=\"226\" \/><\/p>\n<p>Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4\u00b0C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (&gt;98\u00b0C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.<\/p>\n<ul>\n<li><strong>Product name<\/strong><\/li>\n<\/ul>\n<p>Ki 67 Monoclonal Antibody<\/p>\n<ul>\n<li><strong>Immunogen<\/strong><\/li>\n<\/ul>\n<p>Synthetic Peptide<\/p>\n<ul>\n<li><strong>Host<\/strong><\/li>\n<\/ul>\n<p>Mouse<\/p>\n<ul>\n<li><strong>Reactivity<\/strong><\/li>\n<\/ul>\n<p>Human, Mouse, Rat<\/p>\n<ul>\n<li><strong>Applications<\/strong><\/li>\n<\/ul>\n<p>IF, IHC-P<\/p>\n<ul>\n<li><strong>Application notes<\/strong><\/li>\n<\/ul>\n<p>Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:200).<\/p>\n<ul>\n<li><strong>Clonality<\/strong><\/li>\n<\/ul>\n<p>Monoclonal<\/p>\n<ul>\n<li><strong>Isotype<\/strong><\/li>\n<\/ul>\n<p>Mouse IgG1<\/p>\n<ul>\n<li><strong>Purification<\/strong><\/li>\n<\/ul>\n<p>The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen<\/p>\n<ul>\n<li><strong>Formulation<\/strong><\/li>\n<\/ul>\n<p>Liquid solution<\/p>\n<ul>\n<li><strong>Concentration<\/strong><\/li>\n<\/ul>\n<p>1 mg\/ml<\/p>\n<ul>\n<li><strong>Storage buffer<\/strong><\/li>\n<\/ul>\n<p>PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.<\/p>\n<ul>\n<li><strong>Storage instructions<\/strong><\/li>\n<\/ul>\n<p>Stable for one year at -20\u00b0C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.<\/p>\n<p><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=24999\">\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=15880\"><strong><u>CCK-8<\/u><\/strong><strong><u>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=25057\">LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=25127\">\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0<a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=25092\">\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n<p>&nbsp;<\/p>\n<table width=\"724\">\n<tbody>\n<tr>\n<td width=\"121\">\u8ca8\u865f<\/td>\n<td width=\"131\">\u7522\u54c1\u540d\u7a31<\/td>\n<td width=\"123\">\u80de\u5668\u67d3\u8272\u4f4d\u7f6e<\/td>\n<td width=\"145\">\u87a2\u5149\u6ce2\u6bb5<\/td>\n<td width=\"192\"><\/td>\n<\/tr>\n<tr>\n<td width=\"121\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24733\"><strong><u>\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\n<td width=\"131\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24733\">TraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n<td width=\"123\">\u7c92\u7dda\u9ad4(mitochondria)<\/td>\n<td width=\"145\">Ex \/ Em = 490\/523 nm<\/td>\n<td width=\"192\">\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n<tr>\n<td width=\"121\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24758\"><strong><u>\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\n<td width=\"131\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24758\">Pro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n<td width=\"123\">\u5fae\u7ba1\u86cb\u767d(Tubulin)<\/td>\n<td width=\"145\">Ex\/Em = 500\/520 nm<\/td>\n<td width=\"192\">\u6d3b\u7d30\u80de\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de<\/td>\n<\/tr>\n<tr>\n<td width=\"121\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24762\"><strong><u>\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\n<td width=\"131\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24762\">Abbkine\u00a0<\/a><a href=\"http:\/\/biopioneer.com.tw\/?p=24762\">\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n<td width=\"123\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24762\">\u7d30\u80de\u819c<\/a>\u00a0(plasma membrane)<\/td>\n<td width=\"145\">Ex\/Em = 484\/501 nm<\/td>\n<td width=\"192\">\u6d3b\u7d30\u80de\u3001\u56fa\u5b9a\u61f8\u6d6e\u3001\u8cbc\u9644\u7d30\u80de<\/td>\n<\/tr>\n<tr>\n<td width=\"121\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24773\"><strong><u>\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\n<td width=\"131\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24773\">DAPI Staining Solution<\/a><\/td>\n<td width=\"123\">\u7d30\u80de\u6838 dsDNA<\/td>\n<td width=\"145\">Ex\/Em: 358\/461 nm (with DNA)<\/td>\n<td width=\"192\">\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n<tr>\n<td width=\"121\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24764\"><strong><u>\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n<td width=\"131\"><a href=\"http:\/\/biopioneer.com.tw\/?p=24764\">\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\n<td width=\"123\">Lysosome<\/td>\n<td width=\"145\">deep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n<td width=\"192\">\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ki-67\u55ae\u682a\u6297\u9ad4 | \u8ca8\u865fABM40064 \u7d30\u80de\u589e\u6b96(Cell proliferation)\u53ef\u7528\u65bc\u8a55\u4f30\u6b63\u5e38\u7684 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":26582,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1187],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26580"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26580"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26580\/revisions"}],"predecessor-version":[{"id":26585,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26580\/revisions\/26585"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/26582"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26580"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26580"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26580"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}