{"id":26572,"date":"2020-12-14T14:23:14","date_gmt":"2020-12-14T06:23:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26572"},"modified":"2022-07-19T15:42:40","modified_gmt":"2022-07-19T07:42:40","slug":"%e7%a5%9e%e7%b6%93%e5%85%83%e7%b5%84%e7%b9%94%e7%b4%b0%e8%83%9eminute%e5%96%ae%e6%a0%b8%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-invent%e8%b2%a8%e8%99%9f-bn-020","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26572","title":{"rendered":"\u795e\u7d93\u5143\u7d44\u7e54\/\u7d30\u80deMinute\u2122\u55ae\u6838\u5206\u96e2\u8a66\u5291\u76d2| Invent\u8ca8\u865f BN-020"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31<\/strong>:<\/strong> \u795e\u7d93\u5143\u7d44\u7e54\/\u7d30\u80deMinute\u2122\u55ae\u6838\u5206\u96e2\u8a66\u5291\u76d2\uff0820\u6b21\u88fd\u5099\uff09| Invent<\/strong>\u8ca8\u865f<\/strong>\u00a0BN-020<\/a><\/span><\/p>\n

Minute\u2122 Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps)<\/p>\n

\u8a72\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u5f9e\u5927\u591a\u6578\u795e\u7d93\u5143\u7d44\u7e54\/\u57f9\u990a\u7d30\u80de\uff08\u65b0\u9bae\u6216\u51b7\u51cd\uff09\u4e2d\u5206\u96e2\u7d30\u80de\u6838<\/p>\n

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Nuclei Isolated from zebrafish brain tissue Using BN-020 and stained with DAPI (False-coloured in green) (Courtesy Sema Elif Eski, Singh Lab, Universite\u0301 Libre de Bruxelles).<\/strong><\/em><\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> \u5206\u96e2\u7684\u6838(nuclei)\u5ee3\u6cdb\u7528\u65bc\u5404\u7a2e\u5be6\u9a57\uff0c\u4f8b\u5982FACS\uff0c\u55ae\u6838\u5206\u6790\uff08single nucleus analysis, \u4f8b\u5982RNA-seq\u548cATAC-seq\uff09\uff0c\u514d\u75ab\u87a2\u5149\u67d3\u8272(immunofluorescence staining)\uff0c\u7d30\u80de\u9031\u671f(cell cycle)\u548c\u7d30\u80de\u51cb\u4ea1\u7814\u7a76(apoptosis research)\u3002\u55ae\u7d30\u80deRNA-seq(Single cell RNA-seq)\u662f\u7814\u7a76\u7d44\u7e54\u8907\u96dc\u7d30\u80de\u7d44\u6210\u7684powerful\u6280\u8853\u3002\u7136\u800c\uff0c\u795e\u7d93\u5143(Neurons)\u662f\u9ad8\u5ea6\u4e92\u9023\u7684\uff0c\u4e26\u4e14\u5f88\u96e3\u5f9e\u8af8\u5982\u5927\u8166(brain)\u548c\u810a\u9ad3(spinal cord)\u7684\u795e\u7d93\u5143\u7d44\u7e54(neuronal tissues)\u7372\u5f97\u55ae\u7d30\u80de\u3002\u5f9e\u51b7\u51cd\u7684\u795e\u7d93\u5143\u7d44\u7e54(frozen neuronal tissues)\u4e2d\u5206\u96e2\u5b8c\u6574\u7d30\u80de\u751a\u81f3\u66f4\u52a0\u56f0\u96e3\u3002\u7531\u65bc\u9019\u4e9b\u9650\u5236\uff0c\u55ae\u7d30\u80deRNA-seq(single cell RNA-seq)\u88ab\u55ae\u6838-seq\u53d6\u4ee3\u3002\u5f9e\u795e\u7d93\u5143\u7d44\u7e54\u4e2d\u5206\u96e2\u55ae\u6838\u7684\u50b3\u7d71\u65b9\u6cd5\u76f8\u5c0d\u7e41\u7463\u4e14\u8017\u6642\uff0c\u4e26\u4e14\u7531\u65bc\u96e3\u4ee5\u64fa\u812b\u6c61\u67d3\u7684\u9ad3\u78f7\u8102(myelin)\u548c\u5176\u4ed6\u7d30\u80de\u6210\u5206\uff0c\u56e0\u6b64\u6536\u7387\u901a\u5e38\u8f03\u4f4e\u3002\u8a72\u5957\u4ef6\u5c08\u9580\u8a2d\u8a08\u7528\u65bc\u901a\u904e\u7c21\u55ae\uff0c\u5feb\u901f\u548c\u6709\u6548\u7684\u64cd\u4f5c\u65b9\u6cd5\u3002\u5927\u7d0430\u5206\u9418\u5373\u53ef\u7372\u5f97\u9ad8\u5ea6\u7d14\u5316\u7684\u55ae\u6838\u3002\u8207\u50b3\u7d71\u65b9\u6cd5\u76f8\u6bd4\uff0c\u8a72\u8a66\u5291\u76d2\u9700\u8981\u7684\u8d77\u59cb\u539f\u6599\u5c11\u5f97\u591a\uff0c\u4e26\u4e14\u53ef\u4ee5\u8655\u7406\u5927\u7bc4\u570d\u7684\u6a23\u54c1\u91cf\uff081-25 mg\uff09\u3002\u7de9\u885d\u6db2\u5305\u542b\u5c08\u7528\u7684detergents\u6df7\u5408\u7269\uff0c\u53ef\u6709\u6548\u88c2\u89e3\u7d30\u80de\u3002\u5982\u679c\u4e0d\u5e0c\u671b\u4f7f\u7528\u53bb\u6c61\u5291\uff0c\u4e5f\u53ef\u4ee5\u4f7f\u7528\u8ca8\u865fNI-024\u7684\u7121\u53bb\u6c61\u5291\u6838\u5206\u96e2\u8a66\u5291\u76d2\u3002<\/p>\n

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\"\"<\/p>\n

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\"\"<\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

Kit includes:<\/strong><\/p>\n\n\n\n\n\n\n\n
Items<\/strong><\/td>\nQuantity<\/strong><\/td>\n<\/tr>\n
Buffer A<\/td>\n15 ml<\/td>\n<\/tr>\n
Buffer B<\/td>\n25 ml<\/td>\n<\/tr>\n
Pestles for 1.5 ml Tubes<\/td>\n2 units<\/td>\n<\/tr>\n
Filter Cartridge with Collection Tubes<\/td>\n20 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/u><\/td>\nCatalog No.<\/u><\/td>\n<\/tr>\n
\u3000<\/u><\/td>\n\u3000<\/u><\/td>\n<\/tr>\n
Minute TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\nSD-001\/SN-002<\/td>\n<\/tr>\n
Minute TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\nSC-003<\/td>\n<\/tr>\n
Minute TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\nSN-006<\/td>\n<\/tr>\n
Minute TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\nSM-005<\/td>\n<\/tr>\n
Minute TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\nSM-005-p<\/td>\n<\/tr>\n
Minute TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\nMP-007<\/td>\n<\/tr>\n
Minute TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests) NEW!<\/td>\nMM-038<\/td>\n<\/tr>\n
Minute TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\nSD-008\/SN-009<\/td>\n<\/tr>\n
Minute TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\nSN-010<\/td>\n<\/tr>\n
Minute TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\nCP-011<\/td>\n<\/tr>\n
Minute TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\nSB-004<\/td>\n<\/tr>\n
Minute TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\nSC-012<\/td>\n<\/tr>\n
Minute TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\nNE-013<\/td>\n<\/tr>\n
Minute TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\nHP-014<\/td>\n<\/tr>\n
Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYT-015<\/td>\n<\/tr>\n
Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYD-016<\/td>\n<\/tr>\n
Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\nYM-017<\/td>\n<\/tr>\n
Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\nMM-018<\/td>\n<\/tr>\n
Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\nPN-019<\/td>\n<\/tr>\n
MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\nHD-021<\/td>\n<\/tr>\n
MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\nAT-022<\/td>\n<\/tr>\n
MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\nAF-023<\/td>\n<\/tr>\n
MinuteTM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\nNI-024<\/td>\n<\/tr>\n
MinuteTM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps) NEW!<\/td>\nBN-020<\/td>\n<\/tr>\n
MinuteTM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps) NEW!<\/td>\nSN-047<\/td>\n<\/tr>\n
MinuteTM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\nFE-025<\/td>\n<\/tr>\n
MinuteTM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\nEI-027<\/td>\n<\/tr>\n
MinuteTM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\nSE-030<\/td>\n<\/tr>\n
MinuteTM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\nNT-032<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\nLY-034<\/td>\n<\/tr>\n
MinuteTM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\nER-036<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-039<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-042<\/td>\n<\/tr>\n
MinuteTM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPT-040<\/td>\n<\/tr>\n
MinuteTM<\/sup> Nuclear Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPN-041<\/td>\n<\/tr>\n
MinuteTM<\/sup> Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests) NEW!<\/td>\nPF-045<\/td>\n<\/tr>\n
MinuteTM<\/sup> Native Protein Precipitation Kit from Saliva (20 tests) NEW!<\/td>\nSV-043<\/td>\n<\/tr>\n
MinuteTM<\/sup> Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) NEW!<\/td>\nML-044<\/td>\n<\/tr>\n
MinuteTM<\/sup> Rubisco Depletion Kit (20 tests) NEW!<\/td>\nRD-046<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Skin Tissue (50 tests)<\/td>\nSA-01-SK<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Bone Tissue (50 tests)<\/td>\nSA-02-BT<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Blood Vessels (50 tests)<\/td>\nSA-03-BV<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Tendons (50 tests)<\/td>\nSA-04-TD<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Intestines and Mesentery (50 tests)<\/td>\nSA-05-IM<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Muscles (50 tests)<\/td>\nSA-06-MS<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Insects (50 tests)<\/td>\nSA-07-IS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1 \u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | \u8cea\u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a> |<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7522\u54c1\u540d\u7a31: \u795e\u7d93\u5143\u7d44\u7e54\/\u7d30\u80deMinute\u2122\u55ae\u6838\u5206\u96e2\u8a66\u5291\u76d2\uff0820\u6b21\u88fd\u5099\uff09| Invent\u8ca8\u865f\u00a0BN-020 Mi […]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1279],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26572"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26572"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26572\/revisions"}],"predecessor-version":[{"id":26573,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26572\/revisions\/26573"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26572"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26572"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26572"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}