{"id":26539,"date":"2020-12-07T16:43:23","date_gmt":"2020-12-07T08:43:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26539"},"modified":"2025-06-24T14:50:11","modified_gmt":"2025-06-24T06:50:11","slug":"viralentry%e8%bd%89%e5%b0%8e%e5%a2%9e%e5%bc%b7%e5%8a%91-%e7%97%85%e6%af%92%e8%bd%89%e5%b0%8e%e5%a2%9e%e5%bc%b7%e5%8a%91-viralentry-transduction-enhancerg515","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26539","title":{"rendered":"ViralEntry\u2122\u8f49\u5c0e\u589e\u5f37\u5291 | \u75c5\u6bd2\u8f49\u5c0e\u589e\u5f37\u5291 ViralEntry\u2122 Transduction EnhancerG515"},"content":{"rendered":"<p><strong><span style=\"color: #00ccff; font-size: 12pt;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=26539\">ViralEntry\u2122\u75c5\u6bd2\u8f49\u5c0e\u589e\u5f37\u5291 |\u00a0 <\/a><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=26539\">ViralEntry\u2122 Transduction Enhancer<\/a>\u00a0<a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=26539\">abmgood \u8ca8\u865fG515<\/a><\/span><\/strong><\/p>\n<p>Abm\u5c08\u696d\u751f\u7522\u91cd\u7d44\u75c5\u6bd2\u8f09\u9ad4\uff08\u6162\u75c5\u6bd2\uff0c\u817a\u75c5\u6bd2\u548cAAV\uff09\uff0c\u5728\u63d0\u9ad8\u75c5\u6bd2\u8f09\u9ad4\u8f49\u5c0e(virus vector transduction efficiency)\u6548\u7387\u65b9\u9762\u64c1\u670915\u5e74\u4ee5\u4e0a\u7684\u7d93\u9a57\u3002\u7d50\u5408\u5404\u65b9\u75c5\u6bd2\u7d93\u9a57\uff0c\u4e26\u958b\u767c\u4e86\u591a\u7a2e\u75c5\u6bd2\u589e\u5f37\u5291\u88fd\u5291(viral enhancing agents)\uff0c\u53ef\u7528\u65bc\u63d0\u9ad8\u75c5\u6bd2\u8f09\u9ad4\u8f49\u5c0e\u6548\u7387(\u00a0transduction efficiency)\u3002\u6709\u6548\u7684\u75c5\u6bd2\u589e\u5f37\u5291ViralEntry\u2122\uff0c\u4e0d\u50c5\u6709\u6548\u589e\u5f37\u6162\u75c5\u6bd2(\u00a0lentiviral)\u548cAAV\u8f09\u9ad4(AAV vector )\u7684\u8f49\u5c0e\u6548\u7387\u5747\u986f\u8457\u63d0\u9ad8\uff1b\u5305\u62ec\u539f\u4ee3T\u6dcb\u5df4\u7d30\u80de(primary T lymphocytes )\u5728\u5167\u7684\u591a\u7a2e\u7d30\u80de\u985e\u578b\u7684\u8f49\u5c0e\u6548\u7387(transduction efficiency)\u63d0\u9ad8\u4e8610\u500d\u4ee5\u4e0a\u3002\u512a\u65bc\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u985e\u4f3c\u7522\u54c1\u30022020 \u5e74\uff0c\u6211\u5011\u9802\u5c16\u79d1\u5b78\u5bb6\u53d6\u5f97\u4e86\u4e00\u9805\u91cd\u5927\u7a81\u7834\uff0c\u958b\u767c\u51fa\u6700\u6709\u6548\u7684\u75c5\u6bd2\u589e\u5f37\u5291 ViralEntry\u2122\uff0c\u5b83\u4e0d\u50c5\u80fd\u986f\u8457\u63d0\u9ad8\u6162\u75c5\u6bd2\u548c AAV \u8f09\u9ad4\u7684\u8f49\u5c0e\u6548\u7387\uff0c\u9084\u80fd\u5c07\u5305\u62ec\u539f\u4ee3 T \u6dcb\u5df4\u7d30\u80de\u5728\u5167\u7684\u591a\u7a2e\u7d30\u80de\u985e\u578b\u7684\u8f49\u5c0e\u6548\u7387\u63d0\u9ad8 10 \u500d\u4ee5\u4e0a\u3002\u9019\u512a\u65bc\u5e02\u5834\u4e0a\u4efb\u4f55\u540c\u985e\u7522\u54c1\u3002\u5728\u591a\u7a2e\u7d30\u80de\u985e\u578b\u4e2d\uff0c\u75c5\u6bd2\u8f49\u5c0e\u6548\u7387\u63d0\u5347\u9ad8\u9054 10 \u500d\u3002\u5c08\u70ba\u6162\u75c5\u6bd2\u8868\u73fe\u7cfb\u7d71\u8a2d\u8a08\uff0c\u9069\u7528\u65bc\u96e3\u8655\u7406\u7684\u7d30\u80de\u985e\u578b\uff08\u4f8b\u5982 T \u7d30\u80de\u548c B \u7d30\u80de\u3002\u6613\u65bc\u4f7f\u7528\uff1a\u76f4\u63a5\u6dfb\u52a0\u5230\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/12\/ViralEntry\u2122\u8f49\u5c0e\u589e\u5f37\u5291-\u75c5\u6bd2\u8f49\u5c0e\u589e\u5f37\u5291-ViralEntry\u2122-Transduction-EnhancerG5151.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/11\/%E6%93%8D%E4%BD%9C%E6%96%B9%E6%B3%95-300x122.jpg\" alt=\"\u64cd\u4f5c\u65b9\u6cd5\" width=\"148\" height=\"67\" \/><\/a><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/12\/G515.jpg\" alt=\"\" width=\"884\" height=\"370\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/static\/frontend\/Abm\/style\/en_US\/Abm_CatalogPage\/images\/lentivirus\/viralentry-transduction-enhancer-data-1.png\" alt=\"\" \/><\/p>\n<p>abm ViralEntry\u2122\u8f49\u5c0e\u589e\u5f37\u5291\u53ef\u5728\u591a\u7a2e\u7d30\u80de\u4e2d\u5c07\u8f49\u5c0e\u6548\u7387\u63d0\u9ad8100\u500d\u3002 HEK293T\uff0cHeLa\uff0cK562\uff0c\u539f\u4ee3\u4ebaT\u7d30\u80de\u548c\u539f\u4ee3\u8c6c\u813e\u5de8\u566c\u7d30\u80de(Primary Swine Spleen Macrophages)\u7528 Scrambled siRNA GFP \u6162\u75c5\u6bd2\uff08\u8ca8\u865fLVP015-G\uff09\u9032\u884c\u8f49\u5c0e\uff0c\u800c\u4e0d\u4f7f\u7528\u8f49\u5c0e\u589e\u5f37\u5291(transduction enhancer )\uff0c\u800c\u4f7f\u7528Abm\u7684ViralEntry\u2122\u8f49\u5c0e\u589e\u5f37\u5291\uff08\u8ca8\u865fG515\uff09\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/static\/frontend\/Abm\/style\/en_US\/Abm_CatalogPage\/images\/lentivirus\/boosts-transduction-efficiency-icon.png\" alt=\"\" \/><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/12\/G515-2.jpg\" alt=\"\" width=\"897\" height=\"450\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/static\/frontend\/Abm\/style\/en_US\/Abm_CatalogPage\/images\/lentivirus\/works-with-difficult-cells-icon.png\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/static\/frontend\/Abm\/style\/en_US\/Abm_CatalogPage\/images\/lentivirus\/easy-to-use-icon.png\" alt=\"\" \/><\/p>\n<div class=\"abm-block-title\"><strong id=\"block-new-customer-heading\">Features:<\/strong><\/div>\n<div class=\"block-content\">\n<div class=\"abm-check-div\">\n<div class=\"abm-i-div\"><\/div>\n<div class=\"abm-font-div\">Highly Effective<\/div>\n<div class=\"abm-clear-div\">Simple Workflows<\/div>\n<\/div>\n<div class=\"abm-check-div\">\n<div class=\"abm-clear-div\">Consistent Results<\/div>\n<\/div>\n<div class=\"abm-check-div\">\n<div class=\"abm-clear-div\">Wholesale Prices<\/div>\n<div class=\"abm-clear-div\">\n<p><strong>ViralEntry\u2122\u7c21\u6613\u64cd\u4f5c\u624b\u518a\u8aaa\u660e: <\/strong><\/p>\n<p><span style=\"color: #008080;\"><strong>A\uff09\u5c0d\u65bc\u8cbc\u58c1\u548c\/\u6216\u61f8\u6d6e\u7d30\u80de\uff08\u76f4\u63a5\u57f9\u990a-\u9069\u7528\u65bc6\u5b54\u677f\uff09<\/strong><\/span><\/p>\n<p>1.\u7b2c\u4e00\u5929\u3002\u5728\u611f\u67d3\u524d24\u5c0f\u6642\uff0c\u5c07\u60a8\u7684\u76ee\u6a19\u7d30\u80de\u63a5\u7a2e\u57286\u5b54\u677f\u4e2d\u76842\u00d710<sup>5<\/sup>\u7d30\u80de\/\u5b54\u3002<\/p>\n<p>2.\u7b2c2\u5929\u3002\u5c07ViralEntry<sup>TM<\/sup>\u4ee51\uff1a100\u7684\u6bd4\u4f8b\u6dfb\u52a0\u5230\u57f9\u990a\u57fa\u4e2d\uff0c\u4e26\u8f15\u8f15\u6df7\u5408\u3002\u7528\u6bcf\u500b\u5b54\u611f\u67d3\u6700\u4f73MOI\u7684\u91cd\u7d44\u75c5\u6bd2\u3002\u5728\u9069\u7576\u7684\u6eab\u5ea6\uff08\u00b0C\uff09\u4e0b\u5b75\u80b2\u7d30\u80de\uff0c\u7136\u5f8c\u4e8c\u6c27\u5316\u78b3\uff08\uff05\uff09 \u689d\u4ef6\u3002MOI =\u6240\u7528\u75c5\u6bd2\u7684\u611f\u67d3\u55ae\u4f4d\/\u7d30\u80de\u6578\u3002<\/p>\n<p>3.\u7b2c4\u5929\u3002\u66f4\u63db\u751f\u9577\u57f9\u990a\u57fa\u3002\u5982\u679c\u91cd\u7d44\u75c5\u6bd2\u5305\u542b\u8010\u85e5\u57fa\u56e0\uff0c\u70ba\u4e86\u7522\u751f\u7a69\u5b9a\u7684\u7d30\u80de\u514b\u9686\uff0c\u5c07\u7d30\u80de\u50b3\u4ee3\u57f9\u990a\u523010\u5398\u7c73\u57f9\u990a\u76bf\u4e2d\u9032\u884c\u85e5\u7269\u9078\u64c7\u3002\u5982\u679c\u91cd\u7d44\u75c5\u6bd2\u5305\u542b\u87a2\u5149\u6a19\u7c64\uff0c\u53ef\u4ee5\u8a55\u4f30\u8f49\u5c0e\u6548\u7387\u901a\u904e\u5728\u87a2\u5149\u986f\u5fae\u93e1\u4e0b\u6aa2\u67e5\u8a0a\u865f\u3002<\/p>\n<p><strong><span style=\"color: #008080;\">B\uff09\u5c0d\u65bc\u61f8\u6d6e\u7d30\u80de\uff08\u65cb\u63a5\u7a2e-\u9069\u7528\u65bc24\u5b54\u677f\uff09T-\u7d30\u80de\u5efa\u8b70\u4f7f\u7528\u6b64\u65b9\u6cd5<\/span><\/strong><\/p>\n<p>\u7b2c\u4e00\u5929<\/p>\n<p>a\u7528\u9069\u7576\u7684\u751f\u9577\u57f9\u990a\u57fa\u7a00\u91cbViralEntry<sup>TM<\/sup> 1\uff1a100\u3002\u8f15\u8f15\u6df7\u5408\u3002<\/p>\n<p>b. \u541124\u5b54\u677f\u7684\u4e00\u500b\u5b54\u4e2d\u52a0\u5165400 \u00b5l 1\uff1a100\u589e\u5f37\u5291\u6df7\u5408\u7269\uff0c\u4e26\u653e\u5165CO2\u4e2d\u5b75\u5316\u5668\u3002<\/p>\n<p>c. \u52a01&#215;10<sup>5<\/sup>\u7121\u83cc1.5 ml Eppendorf\u7ba1\u4e2d\u7684\u6d3b\u7d30\u80de\uff0c\u4ee51500 rpm\u65cb\u8f495\u5206\u9418\uff0c\u5438\u51fa\u4e0a\u6e05\u6db2\uff0c\u4e26\u7528100 \u00b5l 1\uff1a100\u91cd\u61f8\u6c89\u6fb1\u589e\u5f37\u5291\u6df7\u5408\u3002<\/p>\n<p>d. \u4ee5\u6700\u4f73MOI\u52a0\u5165\u91cd\u7d44\u75c5\u6bd2\uff0c\u4e26\u8f15\u8f15\u6572\u6253\u6df7\u5408\u3002<\/p>\n<p>e. \u4fdd\u6301\u5ba4\u6eab10\u5206\u9418\uff0c\u540c\u6642\u6bcf2-3\u5206\u9418\u8f15\u8f15\u6572\u6253\u8a66\u7ba1<\/p>\n<p>f. \u4ee53200 rpm\u7684\u8f49\u901f\u65cb\u8f4920\u5206\u9418\u3002<\/p>\n<p>g. \u8f15\u8f15\u5730\u91cd\u61f8\u7d30\u80de\u6c89\u6fb1\u4e26\u5c07\u61f8\u6d6e\u6db2\u8f49\u79fb\u81f324\u5b54\u677f\u5305\u542b400 \u00b5l\u7a00\u91cb\u7684ViralEntryTM\uff08\u6b65\u9a5fb\uff09\u3002\u9069\u7576\u5b75\u80b2\u7d30\u80de\u6eab\u5ea6\uff08\u00b0C\uff09\u548c\u4e8c\u6c27\u5316\u78b3\uff08\uff05\uff09 \u689d\u4ef6\u3002<\/p>\n<p>\u7b2c3\u5929<\/p>\n<p>\u66f4\u63db\u751f\u9577\u57f9\u990a\u57fa\u3002\u5982\u679c\u91cd\u7d44\u75c5\u6bd2\u5305\u542b\u8010\u85e5\u57fa\u56e0\uff0c\u6bcf\u96943-4\u5929\u7528\u5305\u542b\u85e5\u7269\u7684\u4ecb\u8cea\u66ff\u63db\u4ecb\u8cea\u4f86\u958b\u59cb\u85e5\u7269\u9078\u64c7\uff0c\u76f4\u5230\u53ef\u4ee5\u9451\u5b9a\u51fa\u8010\u85e5\u7d30\u80de\u3002\u5982\u679c\u91cd\u7d44\u75c5\u6bd2\u5305\u542b\u87a2\u5149\u6a19\u7c64\uff0c<\/p>\n<p>\u53ef\u4ee5\u901a\u904e\u986f\u5fae\u93e1\u6aa2\u67e5\u87a2\u5149\u4e0b\u7684\u4fe1\u865f\u4f86\u8a55\u4f30\u8f49\u5c0e\u6548\u7387\u3002<\/p>\n<\/div>\n<div class=\"abm-clear-div\"><\/div>\n<\/div>\n<\/div>\n<p><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/static\/frontend\/Abm\/style\/en_US\/Abm_CatalogPage\/images\/lentivirus\/ViralEntry-Transduction-Enhancer-product-img-large.png\" alt=\"\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>ViralEntry\u2122\u75c5\u6bd2\u8f49\u5c0e\u589e\u5f37\u5291 |\u00a0 ViralEntry\u2122 Transduction Enhancer [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":26540,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1285],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26539"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26539"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26539\/revisions"}],"predecessor-version":[{"id":51331,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26539\/revisions\/51331"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/26540"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26539"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26539"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26539"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}