{"id":26492,"date":"2020-12-04T11:57:50","date_gmt":"2020-12-04T03:57:50","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26492"},"modified":"2022-07-19T15:50:32","modified_gmt":"2022-07-19T07:50:32","slug":"sars-cov-2-igm-elisa%e8%a9%a6%e5%8a%91%e7%9b%92-sars-cov-2-igm-elisa-kit%e8%b2%a8%e8%99%9fe4902","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26492","title":{"rendered":"SARS-CoV-2 IgM ELISA\u8a66\u5291\u76d2 | SARS-CoV-2 IgM ELISA Kit\u8ca8\u865fE4902"},"content":{"rendered":"

SARS-CoV-2 IgM ELISA\u8a66\u5291\u76d2 | \u00a0SARS-CoV-2 IgM ELISA Kit\u8ca8\u865f<\/strong>E4902<\/a><\/span><\/p>\n

SARS-CoV-2 IgM ELISA Kit<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\"BioVision<\/p>\n

\u7522\u54c1\u4ecb\u7d39:. \u51a0\u72c0\u75c5\u6bd2(Coronaviruses)\u662f\u5927\u578b\u7684\u8102\u8cea\u5305\u88f9(lipid-enveloped)\u7684\u55ae\u93c8RNA\u75c5\u6bd2(single-stranded RNA viruses)\uff0c\u5df2\u77e5\u53ef\u611f\u67d3\u591a\u7a2e\u5bbf\u4e3b\uff0c\u5305\u62ec\u4eba(humans)\uff0c\u8759\u8760(bats)\uff0c\u5c0f\u9f20(mice)\u548c\u5176\u4ed6\u54fa\u4e73\u52d5\u7269(mammals)\u3002\u5728\u4e03\u7a2e\u5df2\u77e5\u7684\u4eba\u985e\u51a0\u72c0\u75c5\u6bd2(human coronaviruses)\u4e2d\uff0cMERS-CoV\uff0cSARS-CoV-1\u548cSARS-CoV-2\u662f\u6700\u81f4\u75c5\u7684\uff0c\u6703\u5f15\u8d77\u56b4\u91cd\u7684\uff0c\u53ef\u80fd\u81f4\u547d\u7684\u547c\u5438\u9053(fatal respiratory)\u548c\u5fc3\u8840\u7ba1\u75c7\u72c0(cardiovascular symptoms)\u3002\u65b0\u578bSARS-CoV-2\u75c5\u6bd2\u7684\u51fa\u73fe\u662f\u9020\u62102019\u5e74\u51a0\u72c0\u75c5\u6bd2\u75be\u75c5\uff08COVID-19\uff09\u5927\u6d41\u884c\u7684\u539f\u56e0\uff0c\u5df2\u88ab\u8b49\u660e\u662f21\u4e16\u7d00\u6700\u5927\u7684\u516c\u5171\u885b\u751f\u5371\u6a5f(public health crisis)\u3002SARS-CoV-2\u5177\u6709\u301c30 kb\u7684\u57fa\u56e0\u7d44(genome)\uff0c\u7de8\u78bc29\u7a2e\u86cb\u767d\u8cea\uff0c\u5305\u62ec\u7528\u65bc\u75c5\u6bd2\u9ad4\u88dd\u914d\u76844\u7a2e\u7d50\u69cb\u86cb\u767d\u8cea(structural proteins)\uff0c\u5373Envelope (E)\uff0c\u819c\uff08Membrane ,M\uff09\uff0c\u6838\u4ec1\uff08Nucleocapsid ,N\uff09\u548c\u7a57\uff08Spike ,S\uff09\u86cb\u767d\u8cea\u3002\u8de8\u819c\u523a\u86cb\u767d(transmembrane Spike protein)\u662f\u8ca0\u8cac\u5bbf\u4e3b\u7d30\u80de\u7d50\u5408\u548c\u75c5\u6bd2\u9032\u5165\u7684\u7cd6\u86cb\u767d(glycoprotein)\u3002\u5b83\u662f\u5177\u6709\u5169\u500b\u7368\u7279\u4e9e\u57fa\u7684\u7570\u4e09\u805a\u9ad4\u8907\u5408\u7269(heterotrimeric complex)\u3002 S1\u4e9e\u57fa\u5305\u542b\u53d7\u9ad4\u7d50\u5408\u57df(Receptor Binding Domain)\uff0c\u5176\u8207\u4f5c\u70ba\u5bbf\u4e3b\u7d30\u80de\u53d7\u9ad4\u7684\u8840\u7ba1\u7dca\u5f35\u7d20\u8f49\u5316\u91762(Angiotensin-Converting Enzyme 2)\u76f8\u4e92\u4f5c\u7528\uff0c\u800cS2\u4e9e\u57fa\u4ecb\u5c0e\u75c5\u6bd2\u819c\u878d\u5408(S2 subunit mediates viral membrane fusion)\u3002 S\u86cb\u767d\u662f\u9ad8\u5ea6\u514d\u75ab\u539f\u6027\u7684\uff0c\u901a\u5e38\u5728\u75c7\u72c0\u767c\u4f5c\u5f8c5-21\u5929\u4e4b\u9593\u6aa2\u6e2c\u5230\u611f\u67d3\u500b\u9ad4\u7684\u8840\u6e05\u8f49\u5316(seroconversion)\u3002 BioVision\u7684SARS-CoV-2 IgM ELISA\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a(enzyme-linked immunosorbent assay)\uff0c\u65e8\u5728\u9451\u5b9a\u5c0dSARS-CoV-2\u611f\u67d3\u7684\u65e9\u671f\u514d\u75ab\u53cd\u61c9\u3002\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u523a\u7a81\u86cb\u767dS1\u4e9e\u57fa(spike protein S1 subunit)\u4f5c\u70ba\u8a98\u990c\u4f86\u6355\u7372\u7a00\u91cb\u7684\u8840\u6f3f\u6216\u8840\u6e05\u6a23\u54c1\u4e2d\u7684\u6297SARS-CoV-2\u6297\u9ad4(anti-SARS-CoV-2 antibodies)\u3002\u96a8\u5f8c\u8207\u9176\u6a19\u8a18\u7684\u6297\u4ebaIgM\u7db4\u5408\u7269\u548c\u6bd4\u8272\u5e95\u7269\u53cd\u61c9\u7522\u751f\u984f\u8272\uff0c\u8a72\u984f\u8272\u8207\u6297S1\u6297\u9ad4\u7684\u6578\u91cf\u6210\u6bd4\u4f8b\u3002\u901a\u904e\u6bd4\u8f03\u6a23\u54c1\u7684OD450 nm\u8b80\u6578\u8207\u9670\u6027\u5c0d\u7167\u8840\u6e05\u7684OD450 nm\u8b80\u6578\u4f86\u78ba\u5b9a\u6297SARS-CoV-2 S1 IgM\u6297\u9ad4(anti-SARS-CoV-2 antibodies)\u7684\u5b58\u5728\u3002\u967d\u6027\u53cd\u61c9\u63d0\u793a\u8fd1\u671f\u611f\u67d3\uff0c\u56e0\u70baIgM\u6297\u9ad4\u662f\u66b4\u9732\u5f8c\u6700\u5148\u51fa\u73fe\u7684\uff0c\u901a\u5e38\u5728\u611f\u67d3\u5f8c\u5e7e\u5929\u5373\u53ef\u6aa2\u6e2c\u5230\u3002\u8a66\u5291\u76d2\u4e2d\u5305\u542b\u542b\u6709\u91cd\u7d44\u4eba\u55ae\u514b\u9686\u6297S1 IgM\u6297\u9ad4(human monoclonal anti-S1 IgM antibody)\u7684\u967d\u6027\u5c0d\u7167\u8840\u6e05(Positive control serum)\uff0c\u7528\u65bc\u9a57\u8b49\u3002\u8a72\u6e2c\u5b9a\u6cd5\u662f\u4e00\u7a2e\u5feb\u901f\uff0c\u7c21\u4fbf\u4e14\u6e96\u78ba\u7684\u65b9\u6cd5\uff0c\u53ef\u7528\u65bc\u78ba\u5b9a\u4e0d\u9700\u8981\u6536\u96c6\u6216\u8655\u7406\u50b3\u67d3\u6027\u75c5\u6bd2\u7684\u8fd1\u671fSARS-CoV-2\u611f\u67d3\u3002\u8a72\u5957\u4ef6\u662f\u7814\u7a76COVID-19\u6d41\u884c\u75c5\u5b78\uff0c\u60a3\u75c5\u7387\u548c\u767c\u75c5\u7387\u7684\u5de5\u5177\u3002<\/p>\n

\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100\u8acb\u6309\u6b64 <\/a><\/span>(405\u3001450\u3001492\u3001630 nm)<\/p>\n

\"RT-6900\"<\/a><\/p>\n

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 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nE4902-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nBioVision\u2019s SARS-CoV-2 IgM ELISA Kit is an enzyme-linked immunosorbent assay intended for identifying the early immune response to SARS-CoV-2 infection. The assay uses the spike protein S1 subunit as bait to capture anti-SARS-CoV-2 antibodies in diluted plasma or serum samples. Subsequent reaction with an enzyme-labeled anti-human-IgM conjugate and a colorimetric substrate generates color, which is proportional to the amount of anti-S1 antibodies. The presence of anti-SARS-CoV-2 S1 IgM antibodies is determined by comparing the OD450 nm reading of the sample to the OD450 nm reading of the negative control serum. A positive reaction suggests recent infection, as IgM antibodies are the first to appear following exposure and are generally detectable a few days after infection. Positive control serum containing a recombinant human monoclonal anti-S1 IgM antibody is included in the kit for validation. The assay is a quick, easy and accurate method for determination of recent SARS-CoV-2 infection that does not require collection or handling of infectious virus. This kit is a valuable tool to study COVID-19 epidemiology, prevalence and morbidity.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance at 450 nm<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Study of epidemiology, prevalence and morbidity of COVID-19
\n\u2022 Screening of SARS-CoV-2 infection rate in a given population
\n\u2022 Determination of recent\/prior SARS-CoV-2 infection<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 A valuable tool to study COVID-19 epidemiology, prevalence and morbidity.
\n\u2022 The assay is a quick, easy and accurate method for determination of recent SARS-CoV-2 infection.
\n\u2022 The assay does not require collection or handling of infectious virus.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Spike Protein-Coated ELISA Plate
\n\u2022 Wash Buffer (10X)
\n\u2022 Sample Diluent
\n\u2022 Anti-IgM-HRP Conjugate (500X)
\n\u2022 Positive Control Serum
\n\u2022 Negative Control Serum
\n\u2022 TMB Substrate
\n\u2022 Stop Solution
\n\u2022 Plate Sealing Film<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u54c1\u9805<\/td>\n\u6e2c\u91cf\u7269<\/td>\n\u6a23\u54c1\u4f86\u6e90<\/td>\n<\/tr>\n
\u00a0\u8ca8\u865f<\/u><\/strong>E4903<\/a><\/td>\nSARS-CoV-2 Nucleocapsid IgG\u6aa2\u6e2c\u8a66\u5291\u76d2 <\/a><\/td>\n\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u6838\u8863\u6bbc\u86cb\u767d(nucleocapsid protein)\u4f5c\u70ba\u8a98\u990c\u4f86\u6355\u7372(capture)\u7a00\u91cb\u7684\u8840\u6f3f\u6216\u8840\u6e05\u6a23\u54c1\u4e2d\u7684\u6297SARS-CoV-2\u6297\u9ad4\u3002<\/td>\nHuman Serum or Plasma (Heparin or EDTA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>E4902<\/a><\/td>\nSARS-CoV-2 IgM ELISA\u8a66\u5291\u76d2 <\/a><\/td>\n\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u523a\u7a81\u86cb\u767dS1\u4e9e\u57fa(spike protein S1 subunit)\u4f5c\u70ba\u8a98\u990c\u4f86\u6355\u7372\u7a00\u91cb\u7684\u8840\u6f3f\u6216\u8840\u6e05\u6a23\u54c1\u4e2d\u7684\u6297SARS-CoV-2\u6297\u9ad4(anti-SARS-CoV-2 antibodies)\u3002<\/td>\nHuman Serum or Plasma (Heparin or EDTA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>E4877<\/a><\/td>\nSARS-CoV-2 RBD \u6aa2\u6e2c\u8a66\u5291\u76d2 <\/a><\/td>\n\u6e2c\u91cf\u652f\u6c23\u7ba1\u80ba\u6ce1\u704c\u6d17\u6db2(bronchoalveolar lavage fluid)\u548c\u9f3b\u54bd\u62ed\u5b50\u6a23\u54c1(nasopharyngeal swab samples)\u4e2d\u7684RBD\u86cb\u767d\u542b\u91cf\u3002<\/td>\nbronchoalveolar lavage fluid\u548c nasopharyngeal swab samples<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>E4901<\/a><\/td>\nSARS-CoV-2 IgG ELISA\u6aa2\u6e2c\u8a66\u5291 <\/a><\/td>\n\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u523a\u7a81\u86cb\u767dS1\u4e9e\u57fa(spike protein S1 subunit)\u4f5c\u70ba\u8a98\u990c\u4f86\u6355\u7372\u7a00\u91cb\u7684\u8840\u6f3f\u6216\u8840\u6e05\u6a23\u54c1\u4e2d\u7684\u6297SARS-CoV-2\u6297\u9ad4\u3002<\/td>\n\n

Human Serum or Plasma (Heparin or EDTA)<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>E4878<\/a><\/td>\nSARS-CoV-2 Nucleocapsid\u6aa2\u6e2c\u8a66\u5291\u76d2 <\/a><\/td>\n\u6e2c\u91cf\u652f\u6c23\u7ba1\u80ba\u6ce1\u704c\u6d17\u6db2(bronchoalveolar lavage fluid)\u548c\u9f3b\u54bd\u62ed\u5b50(nasopharyngeal swab samples)\u6a23\u54c1\u4e2dNucleocapsid\u86cb\u767d\u7684\u542b\u91cf\u3002<\/td>\nBronchoalveolar lavage fluid and nasopharyngeal samples<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>E4821-100<\/a><\/td>\nSARS-CoV-2\u6838\u86cb\u767dIgG\u6297\u9ad4ELISA\u8a66\u5291\u76d2 <\/a><\/td>\n\u6e2c\u91cfSARS-CoV-2\u6838\u86cb\u767dIgG\u6297\u9ad4<\/td>\nSerum, Plasma<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

SARS-CoV-2 IgM ELISA\u8a66\u5291\u76d2 | \u00a0SARS-CoV-2 IgM ELISA Kit\u8ca8\u865fE4 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1290],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26492"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26492"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26492\/revisions"}],"predecessor-version":[{"id":26519,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26492\/revisions\/26519"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26492"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26492"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26492"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}