{"id":26428,"date":"2020-12-01T11:39:19","date_gmt":"2020-12-01T03:39:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26428"},"modified":"2022-07-19T15:55:29","modified_gmt":"2022-07-19T07:55:29","slug":"atp-sulfurylase-%e8%b2%a8%e8%99%9fp1542","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26428","title":{"rendered":"ATP Sulfurylase | \u8ca8\u865fP1542"},"content":{"rendered":"

ATP Sulfurylase | \u8ca8\u865f<\/strong>P1542<\/a><\/span><\/p>\n

ATP Sulfurylase<\/p>\n

\u7522\u54c1\u4ecb\u7d39: ATP Sulfurylase (EC No. EC2.7.7.4) catalyzes the first committed step in incorporating inorganic SO42- by converting ATP to adenylyl sulfate and pyrophosphate for the incorporation of inorganic SO42- into biomolecules. It is also a key enzyme used in pyrosequencing. ATP\u786b\u9178\u5316\u9176\uff08ATP Sulfurylase , EC\u865fEC2.7.7.4\uff09\u901a\u904e\u5c07ATP\u8f49\u5316\u70ba\u817a\u82f7\u9178\u786b\u9178\u9e7d(adenylyl sulfate)\u548c\u7126\u78f7\u9178\u9e7d(pyrophosphate)\u4ee5\u5c07\u7121\u6a5fSO42-\u647b\u5165\u751f\u7269\u5206\u5b50\u4e2d\uff0c\u5f9e\u800c\u50ac\u5316\u4e86\u7121\u6a5fSO42-\u647b\u5165\u7684\u7b2c\u4e00\u6b65\u3002\u5b83\u4e5f\u662f\u7126\u78f7\u9178\u6e2c\u5e8f(pyrosequencing)\u4e2d\u4f7f\u7528\u7684\u95dc\u9375\u9176\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/strong><\/td>\nSulfate Adenylyltransferase, Met3, ATPS, Methionine-requiring protein 3, Sulfate adenylate transferase<\/td>\n<\/tr>\n
Gene Symbol<\/strong><\/td>\nMET3<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n853466<\/td>\n<\/tr>\n
Accession #<\/strong><\/td>\nP08536<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nE. coli<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLyophilized protein<\/td>\n<\/tr>\n
Physical Form Description<\/strong><\/td>\nLyophilized<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n57.7 kDa (amino acids: 1-511 with N-terminal His-Tag)<\/td>\n<\/tr>\n
Purity by<\/strong><\/td>\n>95%<\/td>\n<\/tr>\n
Biological Activity<\/strong><\/td>\nEnzyme activity assay: The activity of the enzyme is 100 U\/mg. The enzyme\u2019s activity was assayed using coupled hexokinase with BioVision\u2019s PicoProbe\u2122 Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Cat. No. K751).<\/td>\n<\/tr>\n
Reconstitution Instructions<\/strong><\/td>\nReconstitute in 50 mM Tris pH 8, 30% glycerol, and 5 mM MgCl2 to a final concentration of 1 mg\/ml.<\/td>\n<\/tr>\n
Amino Acid Sequence<\/strong><\/td>\nThe sequence is from Saccharomyces cerevisiae. Full-length ATP Sulfurylase (aa 1-511 with N-Terminal His-Tag) (Accession# P08536)<\/td>\n<\/tr>\n
Handling<\/strong><\/td>\nCentrifuge the vial prior to opening.<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00baC<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

 <\/p>\n

http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/05\/Biovision_logo_RGB.gif<\/p>\n

<p><a href=” https:\/\/www.biovision.com\/documentation\/datasheets\/P1542.pdf ” target=”_blank”> ATP Sulfurylase\u64cd\u4f5c\u624b\u518a<\/a><\/p><\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
 <\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

ATP Sulfurylase | \u8ca8\u865fP1542 ATP Sulfurylase \u7522\u54c1\u4ecb\u7d39: ATP Sul […]<\/p>\n","protected":false},"author":3,"featured_media":26429,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452],"tags":[1299],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26428"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26428"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26428\/revisions"}],"predecessor-version":[{"id":26430,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26428\/revisions\/26430"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/26429"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26428"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26428"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26428"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}