{"id":26383,"date":"2020-11-26T15:36:23","date_gmt":"2020-11-26T07:36:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26383"},"modified":"2022-07-19T16:03:26","modified_gmt":"2022-07-19T08:03:26","slug":"pde%e7%a3%b7%e9%85%b8%e4%ba%8c%e9%85%af%e9%85%b6%e6%aa%a2%e6%b8%ac-total-phosphodiesterase-activity-assay-kit%e8%b2%a8%e8%99%9fk927","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26383","title":{"rendered":"PDE\u78f7\u9178\u4e8c\u916f\u9176\u6aa2\u6e2c | Total Phosphodiesterase Activity Assay Kit\u8ca8\u865fK927"},"content":{"rendered":"

PDE\u78f7\u9178\u4e8c\u916f\u9176\u6aa2\u6e2c | Phosphodiesterases\u8ca8\u865f<\/strong>K927<\/a><\/span><\/p>\n

\u78f7\u9178\u4e8c\u916f\u9176\uff08Phosphodiesterases ,PDE\uff09\u662f\u964d\u89e3\u7b2c\u4e8c\u4fe1\u4f7f\u5206\u5b50cAMP\u548ccGMP\u4e2d\u78f7\u9178\u4e8c\u916f\u9375\u7684\u9176\u3002\u670911\u500b\u4e0d\u540c\u7684PDE\u5bb6\u65cf(PDE families)\uff0c\u5b83\u5011\u5728\u4e0d\u540c\u7684\u7d44\u7e54\u4e2d\u8868\u9054\u3002PDE\u53c3\u8207\u591a\u7a2e\u751f\u7406\u904e\u7a0b\uff0c\u4f8b\u5982\u51cb\u4ea1(apoptosis)\uff0c\u795e\u7d93\u5143\u4fe1\u865f\u50b3\u5c0e(neuronal signaling)\u548c\u708e\u75c7(inflammation)\u3002 PDE\u6291\u5236\u5291\u53ef\u6709\u76ca\u65bc\u6cbb\u7642\u5404\u7a2e\u75c5\u7406\u72c0\u6cc1\uff0c\u5305\u62ec\u764c\u75c7(cancer)\uff0c\u963f\u723e\u8328\u6d77\u9ed8\u6c0f\u75c5(Alzheimer\u2019s disease)\uff0c\u54ee\u5598(asthma)\uff0c\u80ba\u52d5\u8108\u9ad8\u58d3(pulmonary hypertension)\uff0c\u52c3\u8d77\u529f\u80fd\u969c\u7919(erectile dysfunction)\u7b49\u3002\u67d0\u4e9bFDA\u6279\u51c6\u7684PDE\u6291\u5236\u5291\u5305\u62ec\u897f\u5730\u90a3\u975e(sildenafil)\uff0c\u963f\u666e\u53f8\u7279(apremilast)\uff0c\u7f85\u6c1f\u53f8\u7279(roflumilast)\uff0c\u897f\u6d1b\u4ed6\u5511(cilostazol)\uff0c\u6f58\u751f\u4e01(dipyridamole)\uff0c\u7c73\u529b\u8fb2(milrinone)\uff0c\u6c28\u529b\u8fb2(amrinone)\uff0cAN-2728\u548c\u963f\u90a3\u683c\u96f7\u5fb7(anagrelide)\u3002BioVision\u70ba\u751f\u7269\u5b78\u7814\u7a76\u63d0\u4f9b\u4e86\u591a\u7a2e\u5c0f\u5206\u5b50PDE\u6291\u5236\u5291\u3002<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u78f7\u9178\u4e8c\u916f\u9176\uff08Phosphodiesterase ,PDE\uff09\u662f\u5207\u5272\u78f7\u9178\u4e8c\u916f\u9375(phosphodiester bond)\u7684\u4e00\u65cf\u9176\u3002\u9019\u4e9b\u5305\u62ec\u74b0\u72c0\u6838\u82f7\u9178\u78f7\u9178\u4e8c\u916f\u9176(cyclic nucleotide phosphodiesterases)\uff0c\u78f7\u8102\u9176C(phospholipase C)\u548cD\uff0cRNase\uff0cDNase\u548c\u67d0\u4e9b\u9650\u5236\u6027\u6838\u9178\u5167\u5207\u9176(restriction endonucleases)\u3002\u78f7\u9178\u4e8c\u916f\u9176\uff08Phosphodiesterase ,PDE\uff09\u662f\u7528\u65bc\u591a\u7a2e\u751f\u7269\u5b78\u529f\u80fd\u7684\u91cd\u8981\u9176\uff0c\u5305\u62ec\u7b2c\u4e8c\u4fe1\u4f7f\u5206\u5b50\uff08\u5982cAMP \/ cGMP\uff09\u7684\u4ee3\u8b1d\uff08\u56e0\u6b64\uff0c\u8abf\u7bc0\u5176\u7d30\u80de\u5167\u6c34\u5e73\uff09\u3002 PDE\u6d3b\u6027\u8ca0\u8cac\u5c07mRNA\u964d\u89e3\u70ba\u51cb\u4ea1\u5f8c\u7684\u86cb\u767d\u8cea\u548cDNA\u3002\u4e00\u4e9bPDE\u7570\u69cb\u9ad4\uff08\u4f8b\u5982PDE 5\u548cPDE 11\uff09\u7531\u65bc\u5b83\u5011\u5206\u5225\u5728\u5fc3\u8840\u7ba1\u7cfb\u7d71(cardiovascular system)\u548c\u816b\u7624\u5f62\u6210(tumor formation)\u4e2d\u7684\u4f5c\u7528\u800c\u6210\u70ba\u6cbb\u7642\u9776\u6a19\u3002 BioVision\u7684PDE\u5206\u6790\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u87a2\u5149\u5206\u6790\u65b9\u6cd5\uff0c\u7528\u65bc\u52d5\u614b\u6e2c\u91cf\u7e3d\u78f7\u9178\u4e8c\u916f\u9176\u6d3b\u6027\u3002\u5b83\u57fa\u65bc\u78f7\u9178\u4e8c\u916f\u9176\u5c0d\u9999\u8c46\u7d20\u985e\u5408\u6210\u5e95\u7269(coumarin based synthetic substrate)\u7684\u88c2\u89e3\uff0c\u4ee5\u751f\u6210\u5728Ex \/ Em = 370\/450 nm\u767c\u51fa\u87a2\u5149\u7684\u7522\u7269\u3002\u5176\u8207\u50b3\u7d71\u65b9\u6cd5\u4f9d\u8cf4\u65bc\u901a\u904e\u78f7\u9178\u4e8c\u916f\u9176\u4f5c\u7528\u91cb\u653e\u7684\u78f7\u9178\u9e7d\u7684\u5b9a\u91cf\u4e0d\u540c; \u56e0\u6b64\uff0c\u6a23\u54c1\u80cc\u666f\u53ef\u4ee5\u5ffd\u7565\u4e0d\u8a08\uff0c\u4e26\u4e14\u4e0d\u6703\u5e72\u64fe\u6e2c\u5b9a\u3002\u5e95\u7269\u5c0d\u78f7\u9178\u4e8c\u916f\u9176\u5177\u6709\u7279\u7570\u6027\uff0c\u4e0d\u6703\u88ab\u4f5c\u7528\u65bc\u7fa7\u57fa\u916f(carboxyl esters)\u7684\u9176\u6216\u78f7\u9178\u9176(phosphatases)\u5207\u5272\u3002\u8a72\u6e2c\u5b9a\u6cd5\u53ef\u6aa2\u6e2c\u5230\u4f4e\u81f30.25 \u00b5U\u7684\u7e3dPDE\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK927-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method – Fluorescence (Ex\/Em 370\/450 nm)
\n\u2022 Species reactivity – Mammalian
\n\u2022 Applications – This assay has a detection sensitivity as low as 0.25 \u00b5U.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em 370\/450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetection and quantificiation of total phosphodiesterase activity in biological samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple, highly sensitive, high-throughput compatible
\n\u2022 Detection and quantificiation of total phosphodiesterase activity in biological samples
\n\u2022 This assay has a detection sensitivity as low as 0.25 \u00b5U.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 PDE Assay Buffer
\n\u2022 PDE Substrate
\n\u2022 Coumarin Standard (50 \u00b5M)
\n\u2022 PDE Positive Control<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/strong><\/td>\nCat. No.<\/strong><\/td>\n<\/tr>\n
Amrinone<\/td>\nB2465<\/a><\/strong><\/td>\n<\/tr>\n
AN-2728<\/td>\nB2220<\/a><\/strong><\/td>\n<\/tr>\n
Anagrelide<\/td>\nB2446<\/a><\/strong><\/td>\n<\/tr>\n
Apremilast<\/td>\n2352<\/a><\/strong><\/td>\n<\/tr>\n
BI-409306<\/td>\nB2377<\/a><\/strong><\/td>\n<\/tr>\n
Cilomilast<\/td>\nB2182<\/a><\/strong><\/td>\n<\/tr>\n
Cilostazol<\/td>\n2393<\/a><\/strong><\/td>\n<\/tr>\n
Dipyridamole<\/td>\nB3221<\/a><\/strong><\/td>\n<\/tr>\n
Milrinone<\/td>\n2163<\/strong><\/a><\/td>\n<\/tr>\n
cAMP Phosphodiesterase Activity Assay Kit (F)<\/td>\nK2013<\/strong><\/a><\/td>\n<\/tr>\n
Total Phosphodiesterase Activity Assay Kit (F)<\/td>\nK927<\/strong><\/a><\/td>\n<\/tr>\n
Roflumilast<\/td>\n2675<\/strong><\/a><\/td>\n<\/tr>\n
Sildenafil citrate<\/td>\n2872<\/a><\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

PDE\u78f7\u9178\u4e8c\u916f\u9176\u6aa2\u6e2c | Phosphodiesterases\u8ca8\u865fK927 \u78f7\u9178\u4e8c\u916f\u9176\uff08Phosphodies […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,3],"tags":[1308],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26383"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26383"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26383\/revisions"}],"predecessor-version":[{"id":26385,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26383\/revisions\/26385"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26383"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26383"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26383"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}