{"id":26321,"date":"2020-11-20T15:12:25","date_gmt":"2020-11-20T07:12:25","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26321"},"modified":"2024-09-26T14:11:33","modified_gmt":"2024-09-26T06:11:33","slug":"tc%e7%b8%bd%e8%86%bd%e5%9b%ba%e9%86%87%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-total-cholesterol-tc-colorimetric-assay-kit%e8%b2%a8%e8%99%9fktb2220","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26321","title":{"rendered":"TC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2 | Total Cholesterol (TC) Colorimetric Assay Kit\u8ca8\u865fKTB2220"},"content":{"rendered":"

TC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u00a0Total Cholesterol (TC) Colorimetric Assay Kit\u8ca8\u865fKTB2220<\/a><\/span><\/strong><\/span><\/p>\n

CheKine\u2122 Total Cholesterol (TC) Colorimetric Assay Kit-\u5feb\u901f\u6aa2\u6e2c\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d44\u7e54\uff0c\u7d30\u80de\u548c\u7d30\u83cc\u4e2d\u7e3d\u81bd\u56fa\u9187(Total Cholesterol, TC)\u6fc3\u5ea6<\/span><\/p>\n

\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u7684\u65b9\u6cd5\u4f86\u6aa2\u6e2c\u5404\u7a2e\u751f\u7269\u6a23\u54c1\uff08\u4f8b\u5982\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d44\u7e54\uff0c\u7d30\u80de\u548c\u7d30\u83cc\uff09\u4e2d\u7684\u7e3d\u81bd\u56fa\u9187(Total Cholesterol, TC)\u6fc3\u5ea6\u3002\u5728\u6e2c\u5b9a\u4e2d\uff0c\u916f\u9176(esterase)\u50ac\u5316\u81bd\u56fa\u9187\u916f(cholesterol esters)\u7684\u6c34\u89e3\uff0c\u751f\u6210\u6e38\u96e2\u81bd\u56fa\u9187\uff08free cholesterol , FC\uff09\u548c\u6e38\u96e2\u8102\u80aa\u9178\uff08free fatty acids , FFA\uff09\u3002\u7136\u5f8c\u81bd\u56fa\u9187\u6c27\u5316\u9176(Cholesterol oxidase)\u50ac\u5316\u6e38\u96e2\u81bd\u56fa\u9187\uff08free cholesterol , FC\uff09\u7522\u751f\u25b34-\u81bd\u56fa\u9187(\u25b34-cholestenone)\u548c\u904e\u6c27\u5316\u6c2b(H2O2)\u3002\u53e6\u5916\uff0c\u904e\u6c27\u5316\u7269\u9176(peroxidase)\u53ef\u4ee5\u50ac\u5316H 2 O 2\uff0c4-\u6c28\u57fa\u5b89\u66ff\u6bd4\u6797(4-aminoantipyrine)\u548c\u82ef\u915a(phenol)\uff0c\u5f62\u6210\u5728500nm\u8655\u5177\u6709\u7279\u5fb5\u5438\u6536\u5cf0\u7684\u7d05\u8272\u918c\u5316\u5408\u7269(red quinone compounds)\u3002\u6a23\u54c1\u4e2d\u7684\u7e3d\u81bd\u56fa\u9187(Total Cholesterol,\u00a0TC)\u8207\u7372\u5f97\u7684\u4fe1\u865f\u6210\u6bd4\u4f8b\u3002\u7e3d\u81bd\u56fa\u9187\uff08Total Cholesterol, TC\uff09\u5305\u62ec\u6e38\u96e2\u81bd\u56fa\u9187(\u00a0free cholesterol )\u548c\u81bd\u56fa\u9187\u916f(cholesterol esters)\u3002\u7d44\u7e54\u7e3d\u81bd\u56fa\u9187\uff08Tissue total cholesterol, TC\uff09\u662f\u6307\u7d44\u7e54\u4e2d\u6240\u6709\u8102\u86cb\u767d(\u00a0lipoproteins )\u6240\u542b\u81bd\u56fa\u9187(\u00a0cholesterol )\u7684\u7e3d\u548c\u3002\u8acb\u4f7f\u7528500nm ELISA reader\u6aa2\u6e2c; \u82e5\u9700\u4f7f\u7528\u5206\u5149\u5149\u5ea6\u8a08, \u9700\u66f4\u63db\u70ba\u5fae\u91cfcuvette\u3002<\/p>\n

<\/p>\n

The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce \u25b34-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.Total cholesterol (TC) includes free cholesterol and cholesterol esters. Tissue total cholesterol (TC) refers to the sum of cholesterol contained in all lipoproteins in the tissue.<\/p>\n

<\/p>\n

(\u6587\u737b\u8cc7\u6599) Serum triglycerides (TG), total cholesterol (TC), high density lipoproteins (HDL), and low density lipoproteins (LDL) were estimated in the five groups that each had eight rats in it. The diabetic rats displayed significantly high levels of serum triglycerides, total cholesterol and LDL, as well as a low level of HDL, as shown in Table 4<\/a>. The treatment of\u00a0C. papaya<\/span>\u00a0alleviated dyslipidemia in the Diab +\u00a0C. papaya<\/span>\u00a0group, which was similar to the metformin treatment. The control rats treated with\u00a0C. papaya<\/span>\u00a0did not show any changes.<\/p>\n

 <\/p>\n

\"\u64cd\u4f5c\u624b\u518a2\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Total Cholesterol (TC) Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nThe kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce \u25b34-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nTC; cholesterol<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 TC Working solution
\n\u2022 Standard<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of Total Cholesterol (TC) content in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria.
\n\u2022 Detailed sample preparation and result calculation methods are provided.
\n\u2022 Simple operation steps.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix or substitute reagents or materials from other kit lots or vendors.
\n\u2022 There are volatile substances in the kit, gloves and masks should be worn during the experiment, and the reagent bottle cap should be tightly closed in time after opening.
\n\u2022 Ensure all reagents and equipment are at the appropriate temperature before starting the assay.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at 4\u00b0C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nTotal cholesterol (TC) includes free cholesterol and cholesterol esters. Tissue total cholesterol (TC) refers to the sum of cholesterol contained in all lipoproteins in the tissue.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nTC; cholesterol<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u6ce8\u610f\u4e8b\u9805\"<\/p>\n

\u5ba2\u6236\u81ea\u5099\u6750\u6599: Materials Required but Not Supplied \u00b7Microplate reader or visible spectrophotometer capable of measuring absorbance at 500 nm \u00b796-well plate or microglass cuvette, precision pipettes, disposable pipette tips \u00b7Ice maker, refrigerated centrifuge, water bath \u00b7Anhydrous ethanol \u00b7Homogenizer (for tissue samples)\u3002<\/p>\n

\"\u64cd\u4f5c\u65b9\u6cd5\"<\/p>\n

KTB2220\u8a66\u5291\u76d2\u53ef\u4ee5\u7528\u65bc\u6aa2\u6e2c\u96de\u86cb\u6a23\u672c<\/span>\uff0c\u86cb\u767d\u6a23\u672c<\/span>\u53ef\u76f4\u63a5\u6e2c\u5b9a; \u86cb\u9ec3\u6a23\u672c<\/span>\u6309\u64cd\u4f5c\u624b\u518a\u4e2d\uff0c\u7d44\u7e54\u6a23\u672c\u8655\u7406\u65b9\u6cd5\u88fd\u5099\u5373\u53ef\u3002(\u5982\u4e0b\u6240\u793a) \u6a23\u54c1\u88fd\u5099\u6ce8\u610f\uff1a\u5efa\u8b70\u4f7f\u7528\u65b0\u9bae\u6a23\u54c1\uff0c\u5982\u4e0d\u7acb\u5373\u6aa2\u6e2c\uff0c\u6a23\u54c1\u53ef\u5728-80\u2103\u4fdd\u5b58\u4e00\u500b\u6708\u3002(\u4e00)\u52d5\u7269\u7d44\u7e54<\/strong><\/span>\uff1a\u7a31\u53d6\u7d040.1g\u7d44\u7e54\uff0c\u52a0\u51651mL\u7121\u6c34\u4e59\u9187\u3002\u5728\u51b0\u4e0a\u5747\u8cea\u5316\u3002 4\u2103 8,000g \u96e2\u5fc3 10 \u5206\u9418\u3002\u4f7f\u7528\u4e0a\u6e05\u6db2\u9032\u884c\u6e2c\u5b9a\uff0c\u4e26\u5c07\u5176\u7f6e\u65bc\u51b0\u4e0a\u9032\u884c\u6e2c\u8a66\u3002(\u4e8c) \u7d30\u80de\u6216\u7d30\u83cc<\/strong><\/span>\uff1a\u6536\u96c6 5\u00d7106.<\/sup>\u5c07\u7d30\u80de\u6216\u7d30\u83cc\u653e\u5165\u96e2\u5fc3\u7ba1\u4e2d\uff0c\u96e2\u5fc3\u5f8c\u68c4\u4e0a\u6e05\uff1b\u52a0\u51651mL\u7121\u6c34\u4e59\u9187\u5728\u51b0\u6d74\u4e2d\u8d85\u8072\u7834\u788e\u7d30\u80de\u6216\u7d30\u83cc5\u5206\u9418\uff08\u529f\u738720%\u6216200W\uff0c\u8d85\u80723s\uff0c\u9593\u96947s\uff0c\u91cd\u890730\u6b21\uff09\u3002 4\u2103 8,000 g \u96e2\u5fc3 10 \u5206\u9418\u3002\u4f7f\u7528\u4e0a\u6e05\u6db2\u9032\u884c\u6e2c\u5b9a\uff0c\u4e26\u5c07\u5176\u7f6e\u65bc\u51b0\u4e0a\u9032\u884c\u6e2c\u8a66\u3002(\u4e09) \u8840\u6e05\uff08\u8840\u6f3f\uff09\u6a23\u54c1<\/strong><\/span>\uff1a\u76f4\u63a5\u6aa2\u6e2c\u3002\u6ce8\uff1a\u5982\u679c\u4ee5\u86cb\u767d\u6fc3\u5ea6\u8a08\u7b97\u542b\u91cf\uff0c\u6700\u597d\u7528Protein Quantification Kit (BCA Assay), Cat #: KTD3001\u5c0d\u7e3d\u86cb\u767d\u9032\u884c\u5b9a\u91cf\u3002<\/p>\n

 <\/p>\n

 <\/p>\n

\"Spectrumlab<\/a>\"102-T\"<\/a>\"0100-01\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

TC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u00a0Total Cholesterol (TC) Colorimetric Assa […]<\/p>\n","protected":false},"author":3,"featured_media":24734,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1322],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26321"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26321"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26321\/revisions"}],"predecessor-version":[{"id":46738,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26321\/revisions\/46738"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24734"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26321"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26321"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26321"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}