{"id":26316,"date":"2020-11-20T12:45:48","date_gmt":"2020-11-20T04:45:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26316"},"modified":"2022-07-19T16:07:19","modified_gmt":"2022-07-19T08:07:19","slug":"%e6%a0%b8%e9%85%b8%e5%a4%96%e5%88%87%e9%85%b6iiiexonuclease-iii-%e8%b2%a8%e8%99%9fe016-10000-u-100-%ce%bcl","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26316","title":{"rendered":"\u6838\u9178\u5916\u5207\u9176III(Exonuclease III) | \u8ca8\u865fE016 10000 U (100 \u03bcl)"},"content":{"rendered":"

\u6838\u9178\u5916\u5207\u9176III(Exonuclease III) | <\/strong>\u8ca8\u865f<\/strong>E016 \u00a010000 U (100 \u03bcl)<\/a><\/span><\/p>\n

abm<\/strong>\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u6838\u9178\u9176 <\/strong>nucleases<\/strong>)<\/strong>\uff0c\u53ef\u88fd\u5099<\/strong>RNA<\/strong>\u548c<\/strong>DNA<\/strong>\uff0c\u4ee5\u4fbf\u5728\u4e0b\u4e00\u4ee3\u5168\u57fa\u56e0\u7d44\u6e2c\u5e8f<\/strong>(<\/strong>whole genome sequencing<\/strong>)<\/strong>\u548c\u6587\u5eab\u69cb\u5efa<\/strong>(<\/strong>library construction<\/strong>)<\/strong>\u4e2d\u7528\u65bc\u5f8c\u7e8c\u61c9\u7528\u3002<\/strong><\/p>\n

\u6838\u9178\u5916\u5207\u9176III(Exonuclease III)\uff0c\u5927\u8178\u687f\u83cc\u5f9e\u5207\u53e3DNA(nicked DNA)\uff0c\u920d\u7aefDNA(blunt end DNA)\uff0c3′-\u51f9\u9677\u672b\u7aef(3\u2032-recessed ends)\u6216\u5c11\u65bc4\u500b\u9e7c\u57fa\u76843′-\u7a81\u51fa\u7aef\u6cbf3’\u21925’\u65b9\u5411\u6d88\u5316\u96d9\u93c8\u9ad4DNA\uff0c\u4e26\u751f\u6210\u6838\u82f75′-\u78f7\u9178\u3002 DNA\u964d\u89e3\u4ee5\u5747\u52fb\u7684\u901f\u5ea6\u9032\u884c\uff0c\u4e26\u5728\u76f8\u53cd\u7684\u93c8\u4e0a\u7522\u751fssDNA\u7247\u6bb5\u3002\u5728\u78ba\u5b9a\u7684\u53cd\u61c9\u689d\u4ef6\u4e0b\uff0c\u53cd\u61c9\u53ef\u7522\u751f\u53ef\u9810\u6e2c\u548c\u53ef\u518d\u73fe\u7d50\u679c\u3002\u9700\u8981\u512a\u5316\u689d\u4ef6\uff0c\u4f8b\u5982\u6eab\u5ea6(temperature)\uff0c\u96e2\u5b50\u5f37\u5ea6(ionic strength)\uff0c\u6a21\u677fDNA(template DNA)\u5e8f\u5217\u548c\u6838\u9178\u5916\u5207\u9176III\u8207DNA\u7684\u6bd4\u4f8b\uff0c\u4ee5\u9069\u5408\u7279\u5b9a\u61c9\u7528\u4ee5\u5be6\u73fe\u6240\u9700\u7684\u5207\u9664\u7387\u3002\u7531\u65bc\u56fa\u6709\u76843′-\u78f7\u9178\u9176\u6d3b\u6027(3\u2032-phosphatase activity)\uff0c\u5916\u5207\u6838\u9178\u9176III(Exonuclease III)\uff0c\u5927\u8178\u687f\u83cc\u4e5f\u80fd\u5920\u5f9e3′-\u78f7\u9178\u672b\u7aef\u964d\u89e3DNA\u3002\u8a72\u9176\u9084\u5177\u6709\u560c\u5464DNA\u6838\u9178\u5167\u5207\u9176\u6d3b\u6027\u4ee5\u53caRNase H\u6d3b\u6027\u3002<\/p>\n

\"\"<\/p>\n

\u5176\u5b83<\/strong>\u5546\u54c1<\/strong>AKT2<\/u><\/strong>\u00a0<\/u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/a> | <\/strong>EGFR<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>HER2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>LIMK1<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>MEK1 (EE)<\/u><\/strong>\u00a0<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>\u00a0<\/u><\/strong>ROCK2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>ROS1 (G2032R)<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nExonuclease III,\u00a0E.coli<\/em>\u00a0digests duplex DNA in the 3\u2032\u21925\u2032 direction from nicked DNA, blunt end DNA, 3\u2032-recessed ends, or 3\u2032-overhangs of less than four bases, and yields nucleoside 5\u2032-phosphates. The DNA degradation proceeds at a uniform rate and produces stretches of ssDNA on the opposite strand. Under defined reaction conditions, the reaction can yield predictable and reproducible digestion results. Conditions such as temperature, ionic strength, template DNA sequence and the Exonuclease III to DNA ratio need to be optimized to suit specific applications to achieve the desired excision rate. Exonuclease III,\u00a0E.coli<\/em>\u00a0is also capable of degrading DNA from 3\u2032-phosphate ends due to intrinsic 3\u2032-phosphatase activity. The enzyme also has apurinic DNA endonuclease activity as well as RNase H activity.<\/td>\n<\/tr>\n
SKU<\/strong><\/td>\nE016<\/td>\n<\/tr>\n
Unit quantity<\/strong><\/td>\n10 000 U (100 \u03bcl)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n
    \n
  • Generation of intermediates for site-directed mutagenesis<\/li>\n
  • Preparation of strand-specific radio labelled probes<\/li>\n
  • Preparation of single-stranded DNA<\/li>\n
  • Preparation of single-stranded templates for dideoxy-sequencing of DNA<\/li>\n
  • Creation of unidirectional deletions in DNA fragments<\/li>\n
  • Note: This product is not suitable for NGS or second strand cDNA synthesis applications.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n100 U\/\u03bcl<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nEnzyme supplied with 10X Reaction Buffer<\/td>\n<\/tr>\n
Storage Condition<\/strong><\/td>\nStore all components at -20\u00b0C.<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n20 mM Tris-HCl (pH 7.5), 100 mM KCl, 1 mM DTT, and 50% (v\/v) Glycerol.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u6838\u9178\u5916\u5207\u9176III(Exonuclease III) | \u8ca8\u865fE016 \u00a010000 U (100 \u03bcl) ab […]<\/p>\n","protected":false},"author":3,"featured_media":26302,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1323],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26316"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26316"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26316\/revisions"}],"predecessor-version":[{"id":26319,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26316\/revisions\/26319"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/26302"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26316"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26316"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26316"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}