{"id":26312,"date":"2020-11-20T11:35:39","date_gmt":"2020-11-20T03:35:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26312"},"modified":"2022-07-19T16:07:29","modified_gmt":"2022-07-19T08:07:29","slug":"rnase-iii-e-coli-%e8%b2%a8%e8%99%9fe020-100-u-100-%ce%bcl","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26312","title":{"rendered":"RNase III, E.coli | \u8ca8\u865fE020 100 U (100 \u03bcl)"},"content":{"rendered":"

RNase III, E.coli | <\/strong>\u8ca8\u865f<\/strong>E020 100 U (100 \u03bcl)<\/a><\/span><\/p>\n

abm<\/strong>\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u6838\u9178\u9176 <\/strong>nucleases<\/strong>)<\/strong>\uff0c\u53ef\u88fd\u5099<\/strong>RNA<\/strong>\u548c<\/strong>DNA<\/strong>\uff0c\u4ee5\u4fbf\u5728\u4e0b\u4e00\u4ee3\u5168\u57fa\u56e0\u7d44\u6e2c\u5e8f<\/strong>(<\/strong>whole genome sequencing<\/strong>)<\/strong>\u548c\u6587\u5eab\u69cb\u5efa<\/strong>(<\/strong>library construction<\/strong>)<\/strong>\u4e2d\u7528\u65bc\u5f8c\u7e8c\u61c9\u7528\u3002<\/strong><\/p>\n

RNase III\uff0c\u5927\u8178\u687f\u83cc\u662f\u4e00\u7a2e\u5167\u5207\u6838\u7cd6\u6838\u9178\u9176(endoribonuclease)\u7279\u7570\u6027\u5730\u5c07dsRNA\u6d88\u5316\u621012-15\u500b\u9e7c\u57fa\u5c0d(12-15 base pairs )sRNA\u7247\u6bb5\uff0c\u8a72\u7247\u6bb5\u5177\u67092\u500b\u62163\u500b\u6838\u82f7\u9178\u76843′-\u7a81\u51fa\u7aef(3\u2032-overhangs)\u67095’\u78f7\u9178(5′ phosphate )’\u7fa5\u57fa\u672b\u7aef(3′ hydroxyl termini ) RNase III<\/p>\n

\"\"<\/p>\n

\u5176\u5b83<\/strong>\u5546\u54c1<\/strong>AKT2<\/u><\/strong>\u00a0<\/u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/a> | <\/strong>EGFR<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>HER2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>LIMK1<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>MEK1 (EE)<\/u><\/strong>\u00a0<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>\u00a0<\/u><\/strong>ROCK2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>ROS1 (G2032R)<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nRNase III,\u00a0E.coli<\/em>\u00a0is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3\u2032-overhangs with 5′ phosphate and 3′ hydroxyl termini.<\/td>\n<\/tr>\n
SKU<\/strong><\/td>\nE020<\/td>\n<\/tr>\n
Unit quantity<\/strong><\/td>\n100 U (100 \u03bcl)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 dsRNA digestion to short dsRNA fragments
\n\u2022 Useful for RNA processing, maturation and structure studies
\n\u2022 Directed cleavage of RNA
\n\u2022 siRNA preparation for RNA interference in mammalian cellsNote: This product is not suitable for NGS or second strand cDNA synthesis applications.<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1 U\/\u03bcl<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nEnzyme supplied with 10X Reaction Buffer<\/td>\n<\/tr>\n
Storage Condition<\/strong><\/td>\nStore at -20\u00b0C.<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n50 mM Tris-HCl (pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton\u00ae X-100 and 50% (v\/v) Glycerol.<\/td>\n<\/tr>\n
Note<\/strong><\/td>\n\n

One unit is defined as the amount of RNase III,\u00a0E. coli<\/em>\u00a0that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37\u00b0C in 1X RNase III,\u00a0E.coli<\/em>\u00a0Reaction Buffer.<\/p>\n

This product is distributed for laboratory research only. Caution: Not for diagnostic use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

RNase III, E.coli | \u8ca8\u865fE020 100 U (100 \u03bcl) abm\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u6838\u9178\u9176 […]<\/p>\n","protected":false},"author":3,"featured_media":26302,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1325,1324],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26312"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26312"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26312\/revisions"}],"predecessor-version":[{"id":26315,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26312\/revisions\/26315"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/26302"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26312"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26312"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26312"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}