{"id":26306,"date":"2020-11-20T11:10:28","date_gmt":"2020-11-20T03:10:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26306"},"modified":"2022-07-19T16:07:38","modified_gmt":"2022-07-19T08:07:38","slug":"t5-exonuclease-%e8%b2%a8%e8%99%9fe089-1000-u-100-%c2%b5l","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26306","title":{"rendered":"T5 Exonuclease | \u8ca8\u865fE089 1000 U (100 \u00b5l)"},"content":{"rendered":"

T5 Exonuclease | <\/strong>\u8ca8\u865f<\/strong>E089 1000 U (100 \u00b5l)<\/a><\/span><\/p>\n

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abm<\/strong>\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u6838\u9178\u9176 <\/strong>nucleases<\/strong>)<\/strong>\uff0c\u53ef\u88fd\u5099<\/strong>RNA<\/strong>\u548c<\/strong>DNA<\/strong>\uff0c\u4ee5\u4fbf\u5728\u4e0b\u4e00\u4ee3\u5168\u57fa\u56e0\u7d44\u6e2c\u5e8f\u00a0<\/strong>(<\/strong>whole genome sequencing<\/strong>)<\/strong>\u548c\u6587\u5eab\u69cb\u5efa<\/strong>(<\/strong>library construction<\/strong>)<\/strong>\u4e2d\u7528\u65bc\u5f8c\u7e8c\u61c9\u7528\u3002<\/strong><\/p>\n

T5\u6838\u9178\u5916\u5207\u9176(T5 exonuclease)5’\u81f33’\u65b9\u5411\u4e0a5’\u672b\u7aef\u6216\u7dda\u6027\u6216\u74b0\u72c0dsDNA\u5207\u53e3\u8655\u6838\u82f7\u9178\u7684\u964d\u89e3\u3002\u8a72\u5916\u5207\u6838\u9178\u9176\u5728\u9382\u96e2\u5b50\u5b58\u5728\u4e0b\u4e5f\u5177\u6709ssDNA\u6838\u9178\u5167\u5207\u9176\u6d3b\u6027\uff0c\u4f46\u4e0d\u6703\u964d\u89e3\u8d85\u87ba\u65cbdsDNA(upercoiled dsDNA)<\/p>\n

\u5176\u5b83<\/strong>\u5546\u54c1<\/strong>AKT2<\/u><\/strong>\u00a0<\/u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/a> | <\/strong>EGFR<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>HER2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>LIMK1<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>MEK1 (EE)<\/u><\/strong>\u00a0<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>\u00a0<\/u><\/strong>ROCK2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>ROS1 (G2032R)<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nT5 exonuclease catalyzes the degradation of nucleotides either from the 5\u2019 termini or at nicks of linear or circular dsDNA in a 5\u2019 to 3\u2019 direction. This exonuclease also exhibits ssDNA endonuclease activity in the presence of magnesium ions, but will not degrade supercoiled dsDNA.<\/td>\n<\/tr>\n
SKU<\/strong><\/td>\nE089<\/td>\n<\/tr>\n
Unit quantity<\/strong><\/td>\n1000 U (100 \u00b5l)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n
    \n
  • Degradation of ssDNA, dsDNA linear, circular or nicked, while preserving supercoiled plasmid DNA.<\/li>\n
  • Removal of contaminant denatured DNA isolated during alkaline lysis plasmid purification.<\/li>\n
  • Generation of 3\u2019 overhangs for cloning procedures.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n10000 U\/ml<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nEnzyme supplied with a 10X Reaction Buffer. Incubate at 37\u00b0C.<\/td>\n<\/tr>\n
Storage Condition<\/strong><\/td>\nStore all components at -20\u00b0C.<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol and 0.1% Triton X-100.<\/td>\n<\/tr>\n
Note<\/strong><\/td>\nOne unit is defined as the amount of enzyme necessary to catalyze the release of 1 nmol of acid-soluble nucleotides from double-stranded DNA in a 50 \u00b5l volume in 30 minutes at 37\u00b0C. This product is distributed for laboratory research only. Caution: Not for diagnostic use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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T5 Exonuclease | \u8ca8\u865fE089 1000 U (100 \u00b5l) abm\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u6838\u9178\u9176 nu […]<\/p>\n","protected":false},"author":3,"featured_media":26302,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1326],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26306"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26306"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26306\/revisions"}],"predecessor-version":[{"id":26307,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26306\/revisions\/26307"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/26302"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26306"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26306"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26306"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}