{"id":26301,"date":"2020-11-20T10:38:08","date_gmt":"2020-11-20T02:38:08","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26301"},"modified":"2023-03-03T13:40:42","modified_gmt":"2023-03-03T05:40:42","slug":"dnase-i-rnase-free%e9%9d%9e%e7%89%b9%e7%95%b0%e6%80%a7%e6%a0%b8%e9%85%b8%e5%85%a7%e5%88%87%e9%85%b6-abmgood%e8%b2%a8%e8%99%9fe091","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26301","title":{"rendered":"DNase I (RNAse-Free)\u975e\u7279\u7570\u6027\u6838\u9178\u5167\u5207\u9176 | ABMGOOD\u8ca8\u865fE091"},"content":{"rendered":"

DNase I (RNAse-Free) | \u8ca8\u865fE091<\/a><\/span><\/strong><\/p>\n

abm\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u6838\u9178\u9176(nucleases)\uff0c\u53ef\u88fd\u5099RNA\u548cDNA\uff0c\u4ee5\u4fbf\u5728\u4e0b\u4e00\u4ee3\u5168\u57fa\u56e0\u7d44\u6e2c\u5e8f(whole genome sequencing)\u548c\u6587\u5eab\u69cb\u5efa(library construction)\u4e2d\u7528\u65bc\u5f8c\u7e8c\u61c9\u7528\u3002DNase I\u812b\u6c27\u6838\u7cd6\u6838\u9178\u9176I\uff08Deoxyribonuclease I , DNase I\uff1b EC 3.1.21.1\uff09\u662f\u4e00\u7a2e\u975e\u7279\u7570\u6027\u6838\u9178\u5167\u5207\u9176(non-specific endonuclease)\uff0c\u53ef\u50ac\u5316\u55ae\/\u96d9\u93c8DNA((single\/double-stranded DNA)\uff0c\u67d3\u8272\u8cea(chromatin)\u548cRNA-DNA\u96dc\u4ea4\u9ad4\u4e2d\u78f7\u9178\u4e8c\u916f\u9375(phosphodiester bonds)\u7684\u88c2\u89e3\u3002 DNase I\u5207\u5272DNA\u4ee5\u91cb\u653e\u4e8c\u548c\/\u6216\u5be1\u6838\u82f7\u9178\uff085\u2019-phosphorylated , 5′-\u78f7\u9178\u5316\u548c3\u2019-hydroxylated , 3′-\u7fa5\u57fa\u5316\uff09\u7d42\u7522\u7269\u3002\u8a72\u7522\u54c1\u53ef\u7528\u65bcRNA\u61c9\u7528\u3002<\/p>\n

RNaseOFF<\/strong>\u6838\u7cd6\u6838\u9178\u9176\u6291<\/strong>\u5236<\/strong>\u5291<\/strong><\/a>\u00a0|\u00a0DNase I (RNAse-Free)<\/a>\u00a0|\u00a0DNase I<\/strong><\/a><\/strong><\/p>\n\n\n\n\n\n\n\n
SKU<\/strong><\/td>\nE091<\/td>\n<\/tr>\n
Unit quantity<\/strong><\/td>\n200 U (100 \u03bcl)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Removes DNA from protein preparations and RNA samples
\n\u2022 Mediates nick translation
\n\u2022 Generates random fragments for dideoxy sequencing
\n\u2022 Degrade template DNA following in vitro transcription
\n\u2022 Mediate DNase I foot-printing<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n2 U\/\u03bcl<\/td>\n<\/tr>\n
Note<\/strong><\/td>\n\n

One unit is defined as the amount of DNase I that catalyzes the degradation of 1 \u03bcg of DNA in 10 minutes at 37 \u00b0C into tetranucleotides or smaller fragments.<\/p>\n

This product does not contain any RNase inhibitors, so it is recommended to be used in combination with RNaseOFF Ribonuclease Inhibitor (Cat. G138). This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37\u00b0C, or the residual RNase activity will begin to degrade the RNA.<\/p>\n

This product is distributed for laboratory research only<\/p>\n

Caution: Not for diagnostic use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5176\u5b83<\/strong>\u5546\u54c1<\/strong>AKT2<\/u><\/strong>\u00a0<\/u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/a> | <\/strong>EGFR<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>HER2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>LIMK1<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>MEK1 (EE)<\/u><\/strong>\u00a0<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>\u00a0<\/u><\/strong>ROCK2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>ROS1 (G2032R)<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

DNase I (RNAse-Free) | \u8ca8\u865fE091 abm\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u6838\u9178\u9176(nucleases)\uff0c\u53ef […]<\/p>\n","protected":false},"author":3,"featured_media":26302,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1327],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26301"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26301"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26301\/revisions"}],"predecessor-version":[{"id":39392,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26301\/revisions\/39392"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/26302"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26301"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26301"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26301"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}