{"id":26120,"date":"2020-11-10T15:21:11","date_gmt":"2020-11-10T07:21:11","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26120"},"modified":"2025-08-27T11:07:35","modified_gmt":"2025-08-27T03:07:35","slug":"chekine%e9%b9%bc%e6%80%a7%e7%a3%b7%e9%85%b8%e9%85%b6%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-alkaline-phosphatase-akp-alp-%e8%b2%a8%e8%99%9fktb1700","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26120","title":{"rendered":"CheKine\u2122\u9e7c\u6027\u78f7\u9178\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2 | Alkaline Phosphatase, AKP \/ ALP \u8ca8\u865fKTB1700"},"content":{"rendered":"

CheKine\u2122\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>KTB1700<\/span><\/a><\/span><\/p>\n

\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

CheKine\u2122 Tissue and Blood Alkaline Phosphatase (AKP\/ALP) Colorimetric Assay Kit<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong> AKP \/ ALP\uff08Alkaline Phosphatase\uff09\u662f\u4e00\u7a2e\u542b\u92c5\u7684\u7cd6\u86cb\u767d\u9176(zinc-containing glycoprotease)\uff0c\u53ef\u4ee5\u5728\u9e7c\u6027\u74b0\u5883\u4e2d\u6c34\u89e3\u5404\u7a2e\u5929\u7136\u548c\u5408\u6210\u7684\u78f7\u8102\u55ae\u916f\u5316\u5408\u7269\u3002 AKP \/ ALP\uff08Alkaline Phosphatase\uff09\u5ee3\u6cdb\u5206\u4f48\u65bc\u4eba\u9ad4\u7684\u5404\u500b\u5668\u5b98\uff0c\u4e3b\u8981\u662f\u809d\u81df\u3002\u5728\u9e7c\u6027\u74b0\u5883\u4e2d\uff0cAKP \/ ALP\uff08Alkaline Phosphatase\uff09\u50ac\u5316\u9130\u82ef\u4e8c\u7532\u9178\u4e8c\u9209\u751f\u6210\u6e38\u96e2\u82ef\u915a\u3002\u82ef\u915a\u82074-\u6c28\u57fa\u5b89\u66ff\u6bd4\u6797\u548c\u9435\u6c30\u5316\u9240\u53cd\u61c9\u751f\u6210\u7d05\u8272\u918c\u884d\u751f\u7269\uff0c\u5176\u7279\u5fb5\u662f\u5728510 nm\u8655\u6709\u5149\u5438\u6536\u3002\u5438\u5149\u5ea6\u589e\u52a0\u7387\u53cd\u6620\u4e86AKP\u6d3b\u6027\u3002<\/p>\n

<\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Tissue and Blood Alkaline Phosphatase (AKP\/ALP) Activity Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nIn an alkaline environment, AKP \/ ALP catalyzes phthalate disodium to generate free phenol; phenol reacts with 4-aminoantipyrine and potassium ferricyanide to produce a red quinone derivative, which has characteristic light absorption at 510 nm; the absorbance increase rate reflect AKP activity.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Extraction Buffer
\n\u2022 Chromogen A
\n\u2022 Chromogen B
\n\u2022 Chromogen C
\n\u2022 Standard<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of alkaline phosphatase (AKP\/ALP) activity in tissue lysates supernatants, plasma, serum, urine or purified enzyme samples.
\n\u2022 Sample preparation, experimental steps and result calculation are more detailed and accurate.
\n\u2022 Long shelf life.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Assay kit is intended for research use only. Not for use in diagnostic procedures. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
\n\u2022 Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
\n\u2022 Avoid foaming or bubbles when mixing or reconstituting components.
\n\u2022 Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
\n\u2022 Ensure all reagents and equipment are at the appropriate temperature before assay.
\n\u2022 Chromogen A, Chromogen B and Chromogen C should be kept away from light. Chromogen C cannot be used after it turns blue-green.
\n\u2022 After adding Chromogen C, it must be mixed immediately, otherwise the color development will not be complete.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at 4\u00b0C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nAKP \/ ALP is a zinc-containing glycoprotease that can hydrolyze various natural and synthetic phospholipid monoester compounds in an alkaline environment. AKP \/ ALP is widely distributed in various organs of the human body, mainly the liver.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"instantblue_black_bottle_480x800-002\"\"NEOGEN\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e5\u009c\u0096\"\"abm\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u7522\u54c1\u540d\u7a31<\/td>\n\u80de\u5668\u67d3\u8272\u4f4d\u7f6e<\/td>\n\u87a2\u5149\u6ce2\u6bb5<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n\u7c92\u7dda\u9ad4(mitochondria)<\/td>\nEx \/ Em = 490\/523 nm<\/td>\n\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n\u5fae\u7ba1\u86cb\u767d(Tubulin)<\/td>\nEx\/Em = 500\/520 nm<\/td>\n\u6d3b\u7d30\u80de\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n\u7d30\u80de\u819c<\/a>\u00a0(plasma membrane)<\/td>\nEx\/Em = 484\/501 nm<\/td>\n\u6d3b\u7d30\u80de\u3001\u56fa\u5b9a\u61f8\u6d6e\u3001\u8cbc\u9644\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n\u7d30\u80de\u6838 dsDNA<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\nLysosome<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

CheKine\u2122\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c […]<\/p>\n","protected":false},"author":3,"featured_media":24734,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1361,1360],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26120"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26120"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26120\/revisions"}],"predecessor-version":[{"id":52454,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26120\/revisions\/52454"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24734"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26120"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26120"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26120"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}