{"id":26073,"date":"2020-11-03T10:59:40","date_gmt":"2020-11-03T02:59:40","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26073"},"modified":"2022-07-19T16:15:28","modified_gmt":"2022-07-19T08:15:28","slug":"goat-igg-unlb-%e8%b2%a8%e8%99%9f0109-01-southernbiotech","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=26073","title":{"rendered":"Goat IgG-UNLB | \u8ca8\u865f0109-01 Southernbiotech"},"content":{"rendered":"

Goat IgG-UNLB\u00a0<\/strong>\u540c\u578b\u5c0d\u7167\u7d44<\/strong> \/ <\/strong>\u5206\u578b\u5c0d\u7167\u7d44<\/strong>\u00a0 | <\/strong>\u8ca8\u865f0109-01 |\u898f\u683c 5.0 mg\/mL (\u9ad4\u7a4d2.0ml)<\/strong><\/a><\/span><\/p>\n

Isotype control<\/strong>\u7684<\/strong>\u76ee\u7684<\/strong>: <\/strong>\u78ba\u5b9a\u4e00\u6297\u7684\u7d50\u5408\u662f\u5426\u7279\u7570\u6027\uff1b\u800c\u4e0d\u662f\u975e\u7279\u7570\u6027\u7684Fc\u53d7\u9ad4\u6216\u8207\u5176\u4ed6\u86cb\u767d\u7684\u76f8\u4e92\u4f5c\u7528\u6240\u9020\u6210\u7684Signal\u3002\u9084\u53ef\u4ee5\u7528\u4f86\u7af6\u722d\u6027\u7684\u7d50\u5408\u6297\u9ad4\uff0c\u8207\u529f\u80fd\u963b\u65b7\u6297\u9ad4\u767c\u63ee\u540c\u6a23\u7684\u529f\u80fd\u3002Isotype control\u61c9\u7528\u65bc\u6d41\u6c0f\u7d30\u80de\u5100\u6297\u9ad4\u5206\u578bcontrol\u3001ELISA\u6297\u9ad4\u5206\u578bcontrol\u3001FLISA\u6297\u9ad4\u5206\u578bcontrol \u3001IHC\u6297\u9ad4\u5206\u578bcontrol \u3001IP\u6297\u9ad4\u5206\u578bcontrol \u3001invitro \u6297\u9ad4\u5206\u578bcontrol \u3001invivo\u6297\u9ad4\u5206\u578bcontrol<\/p>\n

\u4f7f\u7528<\/strong>Isotype control<\/strong>\u7684\u539f\u5247<\/strong>:<\/strong> \u540c\u578b\u5c0d\u7167\/\u5206\u578b\u5c0d\u7167\u8981\u8207\u4e00\u6297\u7684\u4f86\u6e90\uff0cIg\u5206\u578b\u548c\u6a19\u8a18\u5b8c\u5168\u4e00\u81f4\u3002\u540c\u578b\u5c0d\u7167\/\u5206\u578b\u5c0d\u7167\uff08Isotype Control\uff09\uff1a\u4f7f\u7528\u8207\u4e00\u6297\u76f8\u540c\u7a2e\u5c6c\u4f86\u6e90\u3001\u76f8\u540c\u4e9e\u578b\u3001\u76f8\u540c\u5291\u91cf\u548c\u76f8\u540c\u7684\u514d\u75ab\u7403\u86cb\u767d\u53ca\u4e9e\u578b\u7684\u514d\u75ab\u7403\u86cb\u767d\uff0c\u7528\u65bc\u6d88\u9664\u7531\u65bc\u6297\u9ad4\u975e\u7279\u7570\u6027\u7d50\u5408\u5230\u7d30\u80de\u8868\u9762\u800c\u7522\u751f\u7684\u80cc\u666f\u67d3\u8272\u3002\u5982\u679c\u4e00\u6297\u662f\u591a\u6297\uff0c\u53ef\u4ee5\u7528an normal serum\uff08\u8207\u4e00\u6297\u76f8\u540c\u7684\u6b63\u5e38\u8840\u6e05\uff09\uff0c\u6b64\u65b9\u6cd5\u5e38\u5728IHC\u67d3\u8272\u4e2d\u5e38\u88ab\u7528\u65bc\u8ca0\u5c0d\u7167\u7d44\uff1b\u5047\u82e5\u591a\u91cd\u87a2\u5149\u67d3\u8272\uff0c\u7531\u65bc\u87a2\u5149\u6a19\u8a18\u55ae\u6297\u7684\u4f86\u6e90\u4e0d\u540c\uff0c\u61c9\u9078\u7528\u76f8\u540c\u4f86\u6e90\u7684\u672a\u6a19\u8a18\u55ae\u6297\u4f5c\u70ba\u540c\u578b\u5c0d\u7167\u4f86 \u8abf\u6574\u80cc\u666f\u67d3\u8272\u3002<\/p>\n

\u4f8b\u5b50\u8aaa\u660e: \u6bd4\u5982\u6aa2\u6e2c\u4e00\u6297\u70ba\u55ae\u6297\u7684mouse anti rat CD11b\uff0cclone OX-42 purified IgG\uff0c\u90a3\u9ebc\u5b83\u7684isotype \u662fmouse IgG2a\uff0c \u6240\u4ee5\u53ef\u4ee5\u7528purified\uff08\u7d14\u5316\u7684\uff09 mouse IgG2a\u4f86\u505aOX-42\u7684\u540c\u578b\u5c0d\u7167\uff08Isotype Control\uff09\u3002<\/p>\n

\u7d30\u80de\u514d\u75ab\u5c08\u5bb6<\/strong>\u6975\u529b\u63a8\u85a6<\/strong>SouthernBiotech<\/strong>\u9ad8\u54c1\u8cea<\/strong>I<\/strong>sotype<\/strong> control<\/strong><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8ceaIsotype control-\u514d\u75ab\u7403\u86cb\u767d\u7cfb\u5217\u7522\u54c1\uff0c\u5177\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001PE\u3001APC\u3001SPRD\u3001PE\/CY5.5\u3001APC\/CY5.5\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u5404\u7a2e\u87a2\u5149\u6a19\u8a8c\u3001\u53e6\u5177\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001\u6216\u672a\u6a19\u8a8c\u53caLow Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Source<\/strong><\/td>\nNormal goat serum<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nUNLB (Unconjugated)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nBorate buffered saline, pH 8.2<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n5.0 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n2.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Quality tested applications for relevant formats include –<\/p>\n

Flow Cytometry\u00a01-4<\/sup>
\nELISA
\nFLISA<\/p>\n

Other referenced applications for relevant formats include –<\/p>\n

Immunohistochemistry-Frozen Sections\u00a05-7<\/sup>
\nImmunohistochemistry-Paraffin Sections\u00a08<\/sup>
\nImmunohistochemistry-Whole Mount\u00a018<\/sup>
\nImmunocytochemistry\u00a019,20<\/sup>
\nImmunoprecipitation\u00a021<\/sup>
\nBlocking\u00a04,9,10<\/sup>
\nIn vitro<\/em>\u00a0Control\u00a011-15<\/sup>
\nIn vivo<\/em>\u00a0Control\u00a016,17<\/sup><\/td>\n<\/tr>\n

Recommended Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2793944<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human\/Rat\/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.<\/p>\n

\"Goat<\/p>\n

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD57-UNLB followed by Goat IgG-UNLB (SB Cat. No. 0109-01; gray) and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01) then Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).<\/p>\n

\"Goat<\/p>\n

CEM\/LAV-1 cell lysate was immunoprecipitated with anti-GAPDH or Goat IgG-UNLB (SB Cat. No. 0109-01). Protein was resolved by electrophoresis, transferred to membrane, and probed with anti-GAPDH followed by a secondary antibody and chemiluminescent detection.<\/p>\n

\"Goat<\/p>\n

Image from Kishimoto N, Onitsuka A, Kido K, Takamune N, Shoji S, Misumi S. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates human immunodeficiency virus type 1 infection. Retrovirology. 2012;9:107. Figure 3(d)<\/p>\n

References<\/strong><\/p>\n

    \n
  1. Harriman GR, Kunimoto DY, Elliott JF, Paetkau V, Strober W. The role of IL-5 in IgA B cell differentiation. J Immunol. 1988;140:3033-39. (FC)<\/strong><\/li>\n
  2. Pospisil R, Young-Cooper GO, Mage RG. Preferential expansion and survival of B lymphocytes based on VH framework 1 and framework 3 expression: “positive” selection in appendix of normal and VH-mutant rabbits. Proc Natl Acad Sci USA. 1995;92:6961-5. (FC)<\/strong><\/li>\n
  3. Milpied P, Renard A, Bruneau J, Mendes-da-Cruz DA, Jacquelin S, Asnafi V, et al. Neuropilin-1 is not a marker of human Foxp3+ Treg. Eur J Immunol. 2009;39:1466-71. (FC)<\/strong><\/li>\n
  4. Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92. (FC, Block)<\/strong><\/li>\n
  5. Sleeman MA, Watson JD, Murison JG. Neonatal murine epidermal cells express a functional multidrug-resistant pump. J Invest Dermatol. 2000;115:19-23. (IHC-FS)<\/strong><\/li>\n
  6. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (IHC-FS)<\/strong><\/li>\n
  7. Menke J, Zeller GC, Kikawada E, Means TK, Huang XR, Lan HY, et al. CXCL9, but not CXCL10, promotes CXCR3-dependent immune-mediated kidney disease. J Am Soc Nephrol. 2008;19:1177-89. (IHC-FS)<\/strong><\/li>\n
  8. Tamura M, Aizawa R, Hori M, Ozaki H. Progressive renal dysfunction and macrophage infiltration in interstitial fibrosis in an adenine-induced tubulointerstitial nephritis mouse model. Histochem Cell Biol. 2009;131:483-90. (IHC-PS)<\/strong><\/li>\n
  9. Boesze-Battaglia K, Besack D, McKay T, Zekavat A, Otis L, Jordan-Sciutto K, et al. Cholesterol-rich membrane microdomains mediate cell cycle arrest induced by Actinobacillus actinomycetemcomitans cytolethal-distending toxin. Cell Microbiol. 2006;8:823-36. (Block)<\/strong><\/li>\n
  10. Pepin AC, Tandon R, Cattori V, Niederer E, Riond B, Willi B, et al. Cellular segregation of feline leukemia provirus and viral RNA in leukocyte subsets of long-term experimentally infected cats. Virus Res. 2007;127:9-16. (Block)<\/strong><\/li>\n
  11. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (In vitro control)<\/strong><\/li>\n
  12. Sato H, Wang D, Kudo A. Dissociation of Pax-5 from KI and KII sites during \u03ba-chain gene rearrangement correlates with its association with the underphosphorylated form of retinoblastoma. J Immunol. 2001;166:6704-10. (In vitro control)<\/strong><\/li>\n
  13. Petlickovski A, Laurenti L, Li X, Marietti S, Chiusolo P, Sica S, et al. Sustained signaling through the B-cell receptor induces Mcl-1 and promotes survival of chronic lymphocytic leukemia B cells. Blood. 2005;105:4820-7. (In vitro control)<\/strong><\/li>\n
  14. Zizzo G, Hilliard BA, Monestier M, Cohen PL. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20. (In vitro control)<\/strong><\/li>\n
  15. Li H, Usas A, Poddar M, Chen C, Thompson S, Ahani B, et al. Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness. PLoS One. 2013;8(6):e64923. (In vitro control)<\/strong><\/li>\n
  16. Lo JC, Chin RK, Lee Y, Kang H, Wang Y, Weinstock JV, et al. Differential regulation of CCL21 in lymphoid\/nonlymphoid tissues for effectively attracting T cells to peripheral tissues. J Clin Invest. 2003;112:1495-505. (In vivo control)<\/strong><\/li>\n
  17. Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1\u03b2. Genes Dev. 2006;20:2527-38. (In vivo control)<\/strong><\/li>\n
  18. L\u00e4mmermann T, Afonso PV, Angermann BR, Wang JM, Kastenm\u00fcller W, Parent CA, et al. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature. 2013;498:371-5. (IHC-WM)<\/strong><\/li>\n
  19. Hauser SP, Waldron JA, Upuda KB, Lipschitz DA. Morphological characterization of stromal cell types in hematopoietically active long-term murine bone marrow cultures. J Histochem Cytochem. 1995;43:371-9. (ICC)<\/strong><\/li>\n
  20. Hsieh-Bonassera ND, Wu I, Lin JK, Schumacher BL, Chen AC, Masuda K, et al. Expansion and redifferentiation of chondrocytes from osteoarthritic cartilage: cells for human cartilage tissue engineering. Tissue Eng Part A. 2009;15:3513-23. (ICC)<\/strong><\/li>\n
  21. Kishimoto N, Onitsuka A, Kido K, Takamune N, Shoji S, Misumi S. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates human immunodeficiency virus type 1 infection. Retrovirology. 2012;9:107. (IP)<\/strong><\/li>\n<\/ol>\n

     <\/p>\n

    \u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

    Goat IgG-UNLB\u00a0\u540c\u578b\u5c0d\u7167\u7d44 \/ \u5206\u578b\u5c0d\u7167\u7d44\u00a0 | \u8ca8\u865f0109-01 |\u898f\u683c 5.0 mg\/mL […]<\/p>\n","protected":false},"author":3,"featured_media":4542,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1373],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26073"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26073"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26073\/revisions"}],"predecessor-version":[{"id":26075,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26073\/revisions\/26075"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4542"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26073"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26073"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26073"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}