{"id":25919,"date":"2020-09-18T18:43:10","date_gmt":"2020-09-18T10:43:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25919"},"modified":"2022-07-19T16:42:04","modified_gmt":"2022-07-19T08:42:04","slug":"human-cellexp-pdgfrbcd140b%e4%ba%ba%e9%a1%9e%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9f7493-50","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25919","title":{"rendered":"Human CellExp\u2122 PDGFRb|CD140b\u4eba\u985e\u91cd\u7d44\u86cb\u767d \u8ca8\u865f7493-50"},"content":{"rendered":"

Human CellExp\u2122 PDGFRb\/CD140b\u4eba\u985e\u91cd\u7d44\u86cb\u767d \u8ca8\u865f<\/strong>7493-50<\/a><\/span><\/p>\n

Human CellExp\u2122 PDGFRb\/CD140b<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u4eba\u8840\u5c0f\u677f\u884d\u751f\u7684\u751f\u9577\u56e0\u5b50\u53d7\u9ad4\u03b2\u591a\u80bd\uff08Human platelet-derived growth factor receptor , PDGFRB\uff09\uff0c\u4e5f\u7a31\u70baJ03278\uff0cM21616\uff0cCD140B\uff0cJTK12\uff0cPDGF-R-beta\u548cPDGFR\uff0c\u662f\u8207PDGFB\u7279\u7570\u6027\u7d50\u5408\u4f75\u5177\u6709\u916a\u6c28\u9178\u86cb\u767d\u6fc0\u9176\u6d3b\u6027(tyrosine-protein kinase activity\u7684)\u53d7\u9ad4\u3002\u8840\u5c0f\u677f\u884d\u751f\u751f\u9577\u56e0\u5b50\uff08PDGF\uff09\u5bb6\u65cf\u6210\u54e1\u7684\u7d30\u80de\u8868\u9762\u916a\u6c28\u9178\u6fc0\u9176\u53d7\u9ad4PDGFR \/ PDGF\u7cfb\u7d71\u5305\u62ec\u5169\u500b\u53d7\u9ad4\uff08PDGFRA\u548cPDGFRB\uff09\u548c\u56db\u500b\u914d\u9ad4\uff08A\uff0cB\uff0cC\u548cD\uff09\uff0c\u53d7\u9ad4PDGFRA\u548cPDGFRB\u5b83\u5011\u5728\u5e8f\u5217\u4e0a\u76f8\u95dc\uff0c\u4e26\u4e14\u90fd\u662f\u53d7\u9ad4\u916a\u6c28\u9178\u6fc0\u9176\uff08RTK\uff09\u7684III\u985e\u4e9e\u578b\u7684\u6210\u54e1\uff1b\u5176\u4ed6III\u985eRTK\u662fCSF1R\uff0cKIT\u548cFLT3\uff1b PDGF\u7684\u7d50\u5408\u8a98\u5c0e\u53d7\u9ad4\u540c\u578b\u548c\u7570\u4e8c\u805a\u5316\u4ee5\u53ca\u4fe1\u865f\u8f49\u5c0e\u3002 \u03b2\u53d7\u9ad4\u5728\u5404\u7a2e\u7d30\u80de\u985e\u578b\u4e2d\u53d7\u5230\u7368\u7acb\u8abf\u7bc0\uff0c\u53ef\u6eb6\u6027\u53ef\u6eb6\u6027PDGFRB\u4ee5\u9ad8\u89aa\u548c\u529b\u7d50\u5408PDGF\uff0c\u662f\u6709\u6548\u7684PDGF\u62ee\u6297\u5291\uff0c\u9019\u4e9b\u914d\u9ad4\u5f62\u6210\u540c\u4e8c\u805a\u9ad4\u6216\u7570\u4e8c\u805a\u9ad4\uff08PDGF-AA\uff0c-AB\uff0c-BB\uff0c-CC\uff0c-DD\uff09\u3002\u56db\u500bPDGF\u5728\u5176monomeric forms\u8655\u65bc\u975e\u6d3b\u52d5\u72c0\u614bPDGF\u8207\u86cb\u767d\u916a\u6c28\u9178\u6fc0\u9176\u53d7\u9ad4PDGF\u53d7\u9ad4\u03b1\u548c-\u03b2\u7d50\u5408\u3002\u9019\u5169\u7a2e\u53d7\u9ad4\u540c\u5de5\u578b\u5728\u7d50\u5408PDGF\u4e8c\u805a\u9ad4\u6642\u6703\u4e8c\u805a\u5316\uff0c\u5f9e\u800c\u5c0e\u81f4\u4e09\u7a2e\u53ef\u80fd\u7684\u53d7\u9ad4\u7d44\u5408\uff0c\u5373-\u03b1\u03b1\uff0c-\u03b2\u03b2\u548c-\u03b1\u03b2.PDGF- CC\u5c08\u9580\u8207PDGFR-\u03b1\u03b1\u548c-\u03b1\u03b2\u76f8\u4e92\u4f5c\u7528\uff0c\u4f46\u4e0d\u8207\u2013\u03b2\u03b2\u76f8\u4e92\u4f5c\u7528\uff0c\u56e0\u6b64\u985e\u4f3c\u65bcPDGF-AB\uff0cPDGF-DD\u4ee5\u9ad8\u89aa\u548c\u529b\u8207PDGFR-\u03b2\u03b2\u7d50\u5408\uff0c\u800c\u8207PDGFR-\u03b1\u03b2\u7684\u7d50\u5408\u7a0b\u5ea6\u8f03\u4f4e\uff0c\u88ab\u8a8d\u70ba\u662fPDGFR -\u03b2\u03b2\u7279\u7570\u6027PDGF-AA\u50c5\u8207PDGFR-\u03b1\u03b1\u7d50\u5408\uff0c\u800cPDGF-BB\u662f\u552f\u4e00\u53ef\u4ee5\u9ad8\u89aa\u548c\u529b\u7d50\u5408\u6240\u6709\u4e09\u7a2e\u53d7\u9ad4\u7d44\u5408\u7684PDGF\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/strong><\/td>\nPDGFRB, PDGFR-B, CD140B, CD-140B, JTK12, JTK-12, PDGF-R-beta, PDGFR, PDGFR1, PDGFR-1, PDGFR-beta, PDGFR-\u03b2, PDGF-R-\u03b2<\/td>\n<\/tr>\n
Gene Symbol<\/strong><\/td>\nPDGFRB<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n5159<\/td>\n<\/tr>\n
Accession #<\/strong><\/td>\nP09619<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nHEK293 cells<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLyophilized<\/td>\n<\/tr>\n
Physical Form Description<\/strong><\/td>\nLyophilized from 0.22 \u03bcm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\nThis protein contains C-terminal polyhistidine tag and has a calculated MW of 57.1 kDa. As a result of glycosylation, DTT-reduced protein migrates as 85-95 kDa polypeptide in SDS-PAGE.<\/td>\n<\/tr>\n
Purity by SDS-PAGE<\/strong><\/td>\n\u226595%<\/td>\n<\/tr>\n
Endotoxin Level<\/strong><\/td>\n<1 EU\/\u03bcg by LAL method<\/td>\n<\/tr>\n
Biological Activity<\/strong><\/td>\nMeasured by its ability to inhibit the biological activity of PDGFBB using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 3-8 \u03bcg\/ml in the presence of 4 ng\/ml rhPDGFBB.<\/td>\n<\/tr>\n
Reconstitution Instructions<\/strong><\/td>\nCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 \u00b5g\/ml. Do not vortex. This solution can be stored at 2-8\u00b0C for up to 1 month. For extended storage, it is recommended to store at -20\u00b0C.<\/td>\n<\/tr>\n
Handling<\/strong><\/td>\nCentrifuge the vial prior to opening.<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Human CellExp\u2122 PDGFRb\/CD140b\u4eba\u985e\u91cd\u7d44\u86cb\u767d \u8ca8\u865f7493-50 Human Cell […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1386],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25919"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25919"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25919\/revisions"}],"predecessor-version":[{"id":25923,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25919\/revisions\/25923"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25919"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25919"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25919"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}