{"id":25917,"date":"2020-09-15T12:33:15","date_gmt":"2020-09-15T04:33:15","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25917"},"modified":"2024-01-29T17:09:34","modified_gmt":"2024-01-29T09:09:34","slug":"%e7%b4%b0%e8%83%9e%e8%bd%89%e5%8c%96%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e8%9e%a2%e5%85%89%e6%b3%95%ef%bc%89-cell-transformation-assay-kit%e8%b2%a8%e8%99%9fk922","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25917","title":{"rendered":"\u7d30\u80de\u8f49\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09 | Cell Transformation Assay Kit\u8ca8\u865fK922 (ABCAM ab235699)"},"content":{"rendered":"

\u7d30\u80de\u8f49\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09 | Cell Transformation Assay Kit\u8ca8\u865fK922\u00a0 (ABCAM ab235699)<\/strong><\/a><\/span><\/p>\n

Cell Transformation Assay Kit<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u8f49\u5316\u7684\u7d30\u80de(Transformed cells)<\/strong>\u53ef\u4ee5\u589e\u6b96(proliferate)\u800c\u4e0d\u9644\u8457\u5728\u8868\u9762\u4e0a\u3002 \u9328\u5b9a\u7368\u7acb\u7d30\u80de\u751f\u9577(Anchorage-independent cell growth)\u662f\u7d30\u80de\u8f49\u5316\u7684\u6a19\u8a8c\u3002 Soft-Agar Assay\/ Colony formation assay\u662f\u4e00\u7a2e\u7528\u65bc\u9ad4\u5916\u7be9\u9078\u7d30\u80de\u8f49\u5316(transformation)\u7684\u50b3\u7d71\u65b9\u6cd5\u3002 \u7136\u800c\uff0c\u9019\u7a2e\u65b9\u6cd5\u5f88\u9577\uff083-4 weeks incubatio\uff09\uff0claborious\u8cbb\u529b\uff08counting colonies\u8a08\u6578\u83cc\u843d\uff09\u548cinconsistent\u4e0d\u4e00\u81f4\uff08\u7531\u65bc\u4e3b\u89c0\u8a08\u6578\uff09\u3002\u8a72\u8a66\u5291\u76d2\u4f7f\u7528\u8207\u6838\u9178\u7d50\u5408\u4f75\u7522\u751f\u7da0\u8272\u87a2\u5149\u7684\u5b9a\u91cf\u67d3\u6599\u3002 \u9019\u7a2e\u55ae\u6b65\u65b9\u6cd5\u662f\u975e\u653e\u5c04\u6027\u4e14\u7c21\u55ae\u7684\uff08\u53ea\u9700\u6dfb\u52a0\u548c\u8b80\u53d6\uff0c\u4e26\u4e14\u4e0d\u9700\u8981\u7e41\u7463\u7684\u5de5\u4f5c\uff0c\u4f8b\u5982\u5c0d\u83cc\u843d\u8a08\u6578\uff09\u3002 \u8a72\u65b9\u6cd5\u5177\u6709\u9ad8\u901a\u91cf\u9069\u61c9\u6027\uff0c\u7dda\u6027\u7bc4\u570d\u5f9e50-60,000\u500b\u7d30\u80de\u4e0d\u7b49\u3002 \u6574\u500b\u6e2c\u5b9a\u53ef\u4ee5\u57287-8\u5929\u5167\u5b8c\u6210\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK922-100 (ABCAM ab235699)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em = 480\/530 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian cells<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Measure cell transformation in response to stimuli
\n\u2022 Screen and characterize compounds that influence cell transformation<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli
\n\u2022 Simple & High throughput-adaptable
\n\u2022 Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Agarose Powder
\n\u2022 DMEM Solution (10X)
\n\u2022 Staining Solution
\n\u2022 Agarose Solubilization Solution
\n\u2022 Quantitative Dye (200X)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

 <\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\u8f49\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09 | Cell Transformation Assay Kit\u8ca8\u865fK922\u00a0 ( […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1387],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25917"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25917"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25917\/revisions"}],"predecessor-version":[{"id":48513,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25917\/revisions\/48513"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25917"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25917"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25917"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}