{"id":25903,"date":"2020-09-10T21:50:13","date_gmt":"2020-09-10T13:50:13","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25903"},"modified":"2022-07-19T16:42:52","modified_gmt":"2022-07-19T08:42:52","slug":"tnf-alpha-%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-recombinant-mouse-tnf-e-coli-abm%e8%b2%a8%e8%99%9fz200219","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25903","title":{"rendered":"TNF-alpha \u91cd\u7d44\u86cb\u767d | Recombinant Mouse TNF (E. coli) ABM\u8ca8\u865fZ200219"},"content":{"rendered":"

TNF-alpha \u91cd\u7d44\u86cb\u767d | Recombinant Mouse TNF (E. coli) ABM\u8ca8\u865fZ200219<\/a><\/span><\/p>\n

TNF\u5728\u88ab\u7d30\u83cc\u8102\u591a\u91a3\u523a\u6fc0\u5f8c\u7531\u5de8\u566c\u7d30\u80de(macrophages)\uff0c\u55ae\u6838\u7d30\u80de(monocytes)\uff0c\u55dc\u4e2d\u6027\u7c92\u7d30\u80de(neutrophils)\uff0cT\u7d30\u80de(T cells)\uff0c\u81ea\u7136\u6bba\u50b7\u7d30\u80de(natural killer cells )\u5206\u6ccc\u3002 \u8868\u9054CD4\u7684\u7d30\u80de\u5206\u6cccTNF-\u03b1\uff0c\u800cCD8\uff08+\uff09\u7d30\u80de\u5247\u5206\u6ccc\u5f88\u5c11\u6216\u4e0d\u5206\u6cccTNF-\u03b1\u3002 \u53d7\u523a\u6fc0\u7684\u5916\u5468\u55dc\u4e2d\u6027\u7c92\u7d30\u80de(neutrophilic granulocytes)\u4e5f\u53d7\u523a\u6fc0\u7684\u7d30\u80de\uff0c\u4ee5\u53ca\u8a31\u591a\u8f49\u5316\u7684\u7d30\u80de\u682a\uff0c\u661f\u5f62\u81a0\u8cea\u7d30\u80de(astrocytes)\uff0c\u5c0f\u81a0\u8cea\u7d30\u80de(microglial cells)\uff0c\u5e73\u6ed1\u808c\u7d30\u80de(\u00a0smooth muscle cells)\u548c\u6210\u7e96\u7dad\u7d30\u80de(fibroblasts )\u4e5f\u5206\u6cccTNF\u3002 \u4eba\u4e73\u4e2d\u4e5f\u542b\u6709\u8a72\u56e0\u5b50\u3002 TNF-\u03b1\u7684\u5408\u6210\u7531\u8a31\u591a\u4e0d\u540c\u7684\u523a\u6fc0\u8a98\u5c0e\uff0c\u5305\u62ec\u5e72\u64fe\u7d20(interferons)\uff0cIL-2\uff0cGM-CSF\uff0cSP\uff0c\u7de9\u6fc0\u80bd(Bradykinin)\uff0c\u514d\u75ab\u8907\u5408\u7269(immune complexes)\uff0c\u74b0\u6c27\u5408\u9176\u6291\u5236\u5291(cyclooxygenase)\u548cPAF\uff08\u8840\u5c0f\u677f\u6d3b\u5316\u56e0\u5b50\uff09\u3002 \u4ebaTNF-\u03b1\u662f17 kDa\u7684\u975e\u7cd6\u57fa\u5316\u86cb\u767d\uff0c\u9577\u5ea6\u70ba157\u500b\u6c28\u57fa\u9178\u3002 \u9f20TNF-\u03b1\u662fN-\u7cd6\u57fa\u5316\u7684\u3002 TNF-\u03b2\u7684\u540c\u6e90\u6027\u7d04\u70ba30\uff05\u3002 TNF-\u03b1\u5f62\u6210\u4e8c\u805a\u9ad4\u548c\u4e09\u805a\u9ad4\u3002<\/p>\n

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Description<\/th>\nTNF is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8(+) cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers.<\/td>\n<\/tr>\n
SKU<\/th>\nZ200219<\/td>\n<\/tr>\n
Growth Factor Size<\/th>\n1.0 mg<\/td>\n<\/tr>\n
Unit quantity<\/th>\n1.0 mg<\/td>\n<\/tr>\n
Family<\/th>\nTNF Super-family<\/td>\n<\/tr>\n
Aliases<\/th>\nTNF-alpha, TNF-a, Tumor necrosis factor ligand superfamily member 2, Cachectin, Tnfa, Tnfsf2, Tnf
\nRecombinant Mouse Tumor Necrosis Factor Alpha (TNF)<\/td>\n<\/tr>\n
Gene Symbol<\/th>\nTNF<\/td>\n<\/tr>\n
Gene ID<\/th>\n21926<\/td>\n<\/tr>\n
Accession Number<\/th>\nP06804<\/td>\n<\/tr>\n
Source<\/th>\nE. coli<\/td>\n<\/tr>\n
Species<\/th>\nMouse (M. musculus)<\/td>\n<\/tr>\n
Appearance<\/th>\nLyophilized Powder<\/td>\n<\/tr>\n
Molecular Weight<\/th>\n17 kDa<\/td>\n<\/tr>\n
Endotoxin Level<\/th>\n<1.0 EU\/\u00b5g of recombinant protein as determined by the LAL method.<\/td>\n<\/tr>\n
Purity<\/th>\n>95% as determined by SDS-PAGE<\/td>\n<\/tr>\n
Formulation<\/th>\nRecombinant mouse TNF alpha was lyophilized from 0.2 \u03bcm filtered PBS solution, pH 7.0.<\/td>\n<\/tr>\n
Function<\/th>\nThe ED(50) was determined cytolysis of murine L929 cells in the presence of Actinomycin D is \u2264 0.05 ng\/mL, corresponding to a specific activity of \u2265 3.0 x 10^7 units\/mg.<\/td>\n<\/tr>\n
Reconstitution<\/th>\nA quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg\/mL. This solution can then be diluted into other buffers.<\/td>\n<\/tr>\n
Storage<\/th>\nThe lyophilized protein is stable for at least one year from date of receipt at -70\u00b0C. Upon reconstitution, this cytokine can be stored in working aliquots at 2\u00b0 – 8\u00b0C for one month, or at -20\u00b0C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze\/thaw cycles.<\/td>\n<\/tr>\n
Usage<\/th>\nFor research use only. Not for diagnostic or therapeutic use.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

TNF-alpha \u91cd\u7d44\u86cb\u767d | Recombinant Mouse TNF (E. coli) ABM\u8ca8\u865fZ […]<\/p>\n","protected":false},"author":3,"featured_media":25905,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,3],"tags":[1389],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25903"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25903"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25903\/revisions"}],"predecessor-version":[{"id":25906,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25903\/revisions\/25906"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/25905"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25903"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25903"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25903"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}