{"id":25889,"date":"2020-09-07T18:34:52","date_gmt":"2020-09-07T10:34:52","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25889"},"modified":"2022-07-19T16:43:39","modified_gmt":"2022-07-19T08:43:39","slug":"mouse-igg2b-unlb%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f0104-01-%e8%a6%8f%e6%a0%bc1-0-ml-1-0-mgml","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25889","title":{"rendered":"Mouse IgG2b-UNLB | \u8ca8\u865f0104-01 \u898f\u683c1.0 mL (1.0 mg\/mL)"},"content":{"rendered":"

Mouse IgG2b-UNLB\u00a0| <\/strong>\u8ca8\u865f<\/strong>0104-01 \u00a0<\/strong>\u898f\u683c<\/strong>1.0 mL \u00a0(1.0 mg\/mL)<\/a><\/span><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

\"\"<\/p>\n

ELISA plate was coated with serially diluted Mouse IgG2b-UNLB (SB Cat. No. 0104-01). Immunoglobulin was detected with Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG2b, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG3, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.<\/div>\n

 <\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0Mouse IgG2b-UNLB\uff0c\u4e0d\u5177\u6a19\u8a8c\u3002\u7de9\u885d\u6db2\u914d\u65b9\u5728Borate buffered saline, pH 8.2\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nA-1<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse IgG2b<\/sub>\u03ba<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nChicken IgA<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nUNLB (Unconjugated)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nBorate buffered saline, pH 8.2<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1.0 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

Flow Cytometry \u2013 Quality tested\u00a07-11<\/sup>
\nELISA \u2013 Quality tested\u00a01-6<\/sup>
\nFLISA \u2013 Quality tested
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a011,12<\/sup>
\nImmunohistochemistry-Paraffin Sections\u00a014<\/sup>
\nIn vitro<\/em>\u00a0Control \u2013 Reported in literature\u00a06<\/sup><\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2793882<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

 <\/p>\n

    \n
  1. Beger E, Deocharan B, Edelman M, Erblich B, Gu Y, Putterman C. A peptide DNA surrogate accelerates autoimmune manifestations and nephritis in lupus-prone mice. J Immunol. 2002;168:3617-26. (ELISA)<\/li>\n
  2. Roopenian DC, Christianson GJ, Sproule TJ, Brown AC, Akilesh S, Jung N, et al. The MHC class I-like IgG receptor controls perinatal IgG transport, IgG homeostasis, and fate of IgG-Fc-coupled drugs. J Immunol. 2003;170:3528-33. (ELISA)<\/li>\n
  3. Maier M, Seabrook TJ, Lemere CA. Modulation of the humoral and cellular immune response in A\u03b2 immunotherapy by the adjuvants monophosphoryl lipid A (MPL), cholera toxin B subunit (CTB) and E. coli enterotoxin LT(R192G). Vaccine. 2005;23:5149-59. (ELISA)<\/li>\n
  4. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Ig\u03b1 serine\/threonines fine-tune Ig \u03b1 tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA)<\/li>\n
  5. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3\/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)<\/li>\n
  6. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)<\/li>\n
  7. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC, in vitro control)<\/li>\n
  8. Cosmi L, Liotta F, Lazzeri E, Francalanci M, Angeli R, Mazzinghi B, et al. Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood. 2003;102:4107-14. (FC)<\/li>\n
  9. Charerntantanakul W, Platt R, Roth JA. Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production. Viral Immunol. 2006;19:646-61. (FC)<\/li>\n
  10. Ghimire TR, Benson RA, Garside P, Brewer JM. Alum increases antigen uptake, reduces antigen degradation and sustains antigen presentation by DCs in vitro. Immunol Lett. 2012;147:55-62. (FC)<\/li>\n
  11. Yoon S, Zhang X, Lee IY, Spencer N, Vo P, Choi YS. CD9 is a novel marker for plasma cell precursors in human germinal centers. Biochem Biophys Res Commun. 2013;431:41-6. (FC, IHC-FS)<\/li>\n
  12. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (IHC-FS)<\/li>\n
  13. Xia Y, Janda A, Eryilmaz E, Casadevall A, Putterman C. The constant region affects antigen binding of antibodies to DNA by altering secondary structure. Mol Immunol. 2013;56:28-37. (Circular dichroism spectroscopy protein control)<\/li>\n
  14. SouthernBiotech published data (IHC-PS)<\/li>\n<\/ol>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Mouse IgG2b-UNLB\u00a0| \u8ca8\u865f0104-01 \u00a0\u898f\u683c1.0 mL \u00a0(1.0 mg\/mL) \u7f8e\u570b\u6297 […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[1393],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25889"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25889"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25889\/revisions"}],"predecessor-version":[{"id":25893,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25889\/revisions\/25893"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25889"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25889"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25889"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}