{"id":25886,"date":"2020-09-07T15:42:48","date_gmt":"2020-09-07T07:42:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25886"},"modified":"2025-10-22T15:59:01","modified_gmt":"2025-10-22T07:59:01","slug":"chekine%e7%b8%bd%e6%8a%97%e6%b0%a7%e5%8c%96%e5%8a%91%e8%83%bd%e5%8a%9b%e5%88%86%e6%9e%90-chekine-total-antioxidant-capacity-tac-assay-kit%e8%b2%a8%e8%99%9fktb1500","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25886","title":{"rendered":"CheKine\u2122\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\u5206\u6790 | CheKine\u2122 Total Antioxidant Capacity (TAC) Assay Kit\u8ca8\u865fKTB1500"},"content":{"rendered":"

CheKine\u2122\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\u5206\u6790 | <\/strong>CheKine\u2122 Total Antioxidant Capacity (TAC) Assay Kit\u8ca8\u865f<\/strong>KTB1500<\/a>\u00a0 \u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/p>\n

\"\"<\/p>\n

Fig. Standard Curve of Total Antioxidant Capacity (TAC) Assay. The y-axis is OD593 and the x-axis is Fe2+ concentration (mM).<\/p>\n

\u6297\u6c27\u5316\u5291(Antioxidants)\u5728\u9632\u6b62\u81ea\u7531\u57fa\u548c\u5176\u4ed6\u6f5b\u5728\u6709\u6bd2\u6c27\u5316\u7269\u8cea\u7684\u5f62\u6210\u548c\u6e05\u9664\u65b9\u9762(scavenging of free radicals )\u767c\u63ee\u8457\u91cd\u8981\u4f5c\u7528\u3002\u6297\u6c27\u5316\u5291(antioxidant species)\u7a2e\u985e\u6709\u4e09\u985e\uff1a\u9175\u7d20\u7cfb\u7d71\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176(GSH reductase)\u3001\u904e\u6c27\u5316\u6c2b\u9176(catalase)\u3001\u904e\u6c27\u5316\u7269\u9176(peroxidase)\u7b49\u3001\u5c0f\u5206\u5b50\u6297\u58de\u8840\u9178(ascorbate, uric acid)\u3001\u5c3f\u9178(uric acid)\u3001\u7a40\u80f1\u7518\u80bd(GSH)\u3001\u7dad\u751f\u7d20E\u7b49\u53ca\u86cb\u767d\u8cea\u767d\u86cb\u767d(albumin)\u3001\u8f49\u9435\u86cb\u767d( transferrin)\u7b49\u3002CheKine\u2122\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\uff08Total Antioxidant Capacity , TAC\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u6613\u7528\u7684\u6bd4\u8272\u6e2c\u5b9a\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\uff0c\u5c3f\u6db2\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u6db2\u548c\u5176\u4ed6\u751f\u7269\u6d41\u9ad4\u4e2d\u7684\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\u3002 \u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cFe3 + -TPTZ\u88ab\u6297\u6c27\u5316\u5291\u9084\u539f\u70baFe2 + -TPTZ\u3002 \u9176\u50ac\u5316\u7684\u53cd\u61c9\u7522\u7269Fe2 + -TPTZ\u53ef\u4ee5\u5728\u6bd4\u8272\u8b80\u6578\u70ba593 nm\u7684\u689d\u4ef6\u4e0b\u9032\u884c\u6e2c\u91cf\u3002\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM\u3002<\/strong>\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: serum, plasma, cell culture supernatants, urine, tissue\/ cell lysates and other biological fluids.<\/p>\n

<\/p>\n

 <\/p>\n

<\/p>\n

(\u6587\u737b\u5f15\u7528\u51fa\u81ea<\/a>) The total antioxidant capacity (TAC) and hydroxyl free radical scavenging capacity assays revealed the ability to remove H2<\/sub>O2<\/sub>\u00a0and \u2013OH, reflecting the antioxidant ability of the targeted reagent. The results in Figure\u00a04A<\/a>\u00a0indicated that the moderate combination with peptide at 600:1 did not weaken the total antioxidant capacity of tFNA.<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n

(\u6539\u826f\u7248) KTB1500\u8a66\u5291\u76d2\u5347\u7d1a\u904e\u7a0b\u4e2d\uff0c\u8003\u616e\u5230\u9019\u500b\u6a19\u66f2\u5728\u5f88\u591a\u7a2e\u60c5\u6cc1\u4e0b\uff0c\u662f\u4e00\u500b\u6bd4\u8f03\u6046\u5b9a\u7684\u7684\u65b9\u7a0b\uff1b\u70ba\u4e86\u65b9\u4fbf\u64cd\u4f5c\uff0c\u7701\u7565\u5ba2\u6236\u81ea\u5df1\u88fd\u4f5c\u6a19\u66f2\u7684\u6b65\u9a5f\u7c21\u6f54 (\u672c\u5957\u7d44\u6c92\u6709\u6a19\u6e96\u54c1, \u53ea\u80fd\u63d0\u4f9bPositive control) \uff0c\u63d0\u4f9b\u4e86\u4e00\u500b\u65b9\u7a0b\uff0c\u8b93\u5ba2\u6236\u76f4\u63a5\u5e36\u5165\u65b9\u7a0b\u8a08\u7b97\u5373\u53ef\uff1b\u5728\u6578\u503c\u8a08\u7b97\u4e0a\u548c\u539f\u4f86\u6c92\u6709\u4efb\u4f55\u5340\u5225\uff0c\u53ea\u662f\u7701\u53bb\u4e86\u81ea\u5df1\u88fd\u4f5c\u6a19\u66f2\u7684\u6b65\u9a5f\uff1b\u5ba2\u6236\u4f7f\u7528\u8d77\u4f86\u66f4\u65b9\u4fbf\u3001\u7701\u6642\u3002
\nStandard curve Calculation formula of TAC: y=4.4664x+0.0685, R2=0.9986, y is Fe
\n2+ concentration (\u03bcmol\/mL), x is theabsorbance change value (\u0394A), Substitute the \u0394ATest into the equation to obtain the y value (\u00b5moL\/mL).<\/p>\n

Ktb1500 Positive Control\u6eb6\u89e3\u5f8c\u7684\u4fdd\u5b58\u6642\u9593(4\u5ea6C), Ascorbic Acid (Positive Control)\u00a0 \u5efa\u8b70\u5118\u91cf1\u5468\u5167\u7528\u5b8c; \u5982\u4e0d\u7acb\u5373\u4f7f\u7528, 4\u2103\u53ef\u4ee5\u4fdd\u5b581\u500b\u6708\uff0c\u5206\u88dd\u4fdd\u5b58\u5728-20\u2103, \u53ef\u5132\u5b582-3\u500b\u6708; \u82e5\u6eb6\u6db2\u8b8a\u6210\u68d5\u8272\uff0c\u8aaa\u660e\u7d44\u5206\u8b8a\u8cea.<\/p>\n\n\n\n\n\n\n\n\n
Kit components<\/strong><\/td>\n\u2022 Assay Buffer (10X)
\n\u2022 Substrate Diluent
\n\u2022 Substrate
\n\u2022 Reaction Buffer
\n\u2022 ascorbic acid (positive control)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of Total Antioxidant Capacity in serum, plasma, cell\/tissue samples, urine and bacteria samples.
\n\u2022 Using a stronger antioxidant, sascorbic acid as positive control, instead of the frequently-used Trolox.
\n\u2022 The reaction is carried out under acidic conditions, which can inhibit some endogenous interference factors.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.
\n\u2022 Fresh samples are necessary for good results. If not assayed immediately, samples can be stored at -80\u00b0C for one month.
\n\u2022 Can not use EDTA as the anticoagulant for serum and plasma samples. The samples also can not contain DTT, Mercaptoethanol, Tween, Triton, NP-40.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at -20\u00b0C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

CheKine\u2122\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\u5206\u6790 | CheKine\u2122 Total Antioxidant Capacity […]<\/p>\n","protected":false},"author":3,"featured_media":24734,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1394],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25886"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25886"}],"version-history":[{"count":8,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25886\/revisions"}],"predecessor-version":[{"id":48994,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25886\/revisions\/48994"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24734"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25886"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25886"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25886"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}