{"id":25867,"date":"2020-09-02T15:38:49","date_gmt":"2020-09-02T07:38:49","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25867"},"modified":"2023-10-24T12:58:40","modified_gmt":"2023-10-24T04:58:40","slug":"cck-8%e7%b4%b0%e8%83%9e%e5%a2%9e%e6%ae%96%e5%92%8c%e7%b4%b0%e8%83%9e%e6%af%92%e6%80%a7%e8%a9%a6%e5%8a%91%e7%9b%92-abbkine%e8%b2%a8%e8%99%9fkta1020","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25867","title":{"rendered":"CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2 | Cell Counting Kit-8 (CCK-8) ABBKINE\u8ca8\u865fKTA1020"},"content":{"rendered":"

CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027<\/strong>\u8a66\u5291\u76d2<\/strong>\u00a0|\u00a0<\/strong>\u8ca8\u865f<\/strong>\u00a0KTA1020<\/a>\u00a0\u00a0\u00a0\u5438\u6536\u5149450 nm\u00a0<\/span>\u00a0\u00a0\u00a0CCK8\u64cd\u4f5c\u624b\u518a<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n

CCK-8; WST-8; CCK8; WST8<\/span><\/p>\n

\"LINE\u7fa4\u767c<\/p>\n

Abbkine Cell Counting Kit-8\uff08CCK-8\uff09\u8a2d\u8a08\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u3002<\/strong><\/span><\/p>\n

\u7d30\u80de\u8a08\u6578\u8a66\u5291\u76d2-8\uff08CCK-8\uff09\u4f7f\u7528\u9ad8\u5ea6\u6c34\u6eb6\u6027\u7684\u56db\u5511\u9e7dWST-8\uff1a[2-\uff082-\u7532\u6c27\u57fa-4-\u785d\u57fa\u82ef\u57fa\uff09-3-\uff084-\u785d\u57fa\u82ef\u57fa\uff09-5-\uff082,4-\u4e8c\u78fa\u57fa\u82ef\u57fa \uff09-2H-\u56db\u5511\u9393\u55ae\u9209\u9e7d]\u901a\u904e\u9084\u539f\u8f49\u5316\u70ba\u6c34\u6eb6\u6027\u6a59\u8272\u7532dye\u67d3\u6599\u3002 CCK-8\u5177\u6709\u4f4e\u6bd2\u6027\uff0c\u4e26\u4e14\u5141\u8a31\u7528\u65bc\u6e2c\u5b9a\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u4e2d\u6d3b\u7d30\u80de\u6578\u91cf\u7684\u9748\u654f\u6bd4\u8272\u6e2c\u5b9a\u3002 \u7d30\u80de\u4e2d\u812b\u6c2b\u9176\u7522\u751f\u7684\u7532dye\u67d3\u6599\u7684\u91cf\u8207\u6d3b\u7d30\u80de\u7684\u6578\u91cf\u6210\u6b63\u6bd4\u3002Cell Counting Kit-8\uff08CCK-8\uff09\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u589e\u6b96(cell proliferation)\u53ca\u7d30\u80de\u6bd2\u6027(cell toxicity)\uff0c\u5176\u539f\u7406\u57fa\u65bcWST-8\u7d93\u7d30\u80de\u4e2d\u7684\u812b\u6c2b\u9176\u964d\u4f4e\uff0c\u5f97\u5230\u6a59\u8272\u7522\u7269\uff08formazan\uff09\u3002\u7d30\u80de\u4e2dformazan\u7684\u542b\u91cf\u8207\u6d3b\u7d30\u80de\u6578\u6210\u6b63\u6bd4\u3002\u7d30\u80de\u8a08\u6578\u8a66\u5291\u76d28\uff08Cell Counting Kit-8, CCK-8\uff09\u901a\u904e\u4f7f\u7528\u9ad8\u5ea6\u6c34\u6eb6\u6027\u7684\u56db\u5511\u9e7d-WST-8\u9032\u884c\u975e\u5e38\u65b9\u4fbf\u7684\u6e2c\u5b9a\u3002[2-\uff082-\u7532\u6c27\u57fa-4-\u785d\u57fa\u82ef\u57fa\uff09-3-\uff084-\u785d\u57fa\u82ef\u57fa\uff09-5- \uff082,4-\u4e8c\u78fa\u57fa\u82ef\u57fa\uff09-2H-\u56db\u5511\u9393\u9e7d]\u5728\u96fb\u5b50\u4ecb\u9ad4\u7684\u5b58\u5728\u4e0b\u9084\u539f\u751f\u6210\u6c34\u6eb6\u6027formazan dye\u67d3\u6599\u3002 CCK-8\u662f\u975e\u653e\u5c04\u6027\u7684\uff0c\u53ef\u9032\u884c\u654f\u611f\u7684\u6bd4\u8272\u6e2c\u5b9a\uff0c\u4ee5\u6e2c\u5b9a\u7d30\u80de\u589e\u6b96(cell proliferation)\u548c\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u4e2d\u7684\u6d3b\u7d30\u80de\u6578\u91cf\u3002 WST-8\u88ab\u7d30\u80de\u4e2d\u7684\u812b\u6c2b\u9176(dehydrogenases)\u9084\u539f\uff0c\u5f97\u5230\u6a59\u8272\u7522\u7269(formazan)\uff0c\u53ef\u6eb6\u65bc\u7d44\u7e54\u57f9\u990a\u57fa\u3002\u7d30\u80de\u4e2d\u812b\u6c2b\u9176(dehydrogenases) \u7522\u751f\u7684for<\/strong>mazan dye\u67d3\u6599\u7684\u91cf\u8207\u6d3b\u7d30\u80de\u7684\u6578\u91cf\u6210\u6b63\u6bd4\u3002WST-8\u751f\u7522\u7684\u7522\u54c1(formazan)\u662f\u6c34\u6eb6\u6027\u7684\uff0c\u4e0d\u9700\u8981\u6709\u6a5f\u6eb6\u5291\u6216\u540c\u4f4d\u7d20\u3002\u800c\u4e14formazan\u662f\u7a69\u5b9a\u548c\u5b89\u5168\u7684\u3002\u4f7f\u7528CCK-8\u7684\u6aa2\u6e2c\u9748\u654f\u5ea6\u9ad8\u65bc\u4f7f\u7528\u5176\u4ed6\u56db\u5511\u9e7d\uff08tetrazolium salts, \u5982MTT\uff0cXTT\uff0cMTS\u6216WST-1\uff09\u7684\u6aa2\u6e2c\u9748\u654f\u5ea6\u3002<\/p>\n

\"LINE\u7fa4\u767c(E-1000UV)\"<\/p>\n

\"\"<\/p>\n

Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8.[2-(2- methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. The product (formazan) produced by WST-8 is water soluble, no organic solvents or isotopes required. And the formazan is stable and safe. The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.<\/p>\n

\"\"<\/p>\n

Fig. Abbkine Economical Cell Counting Kit-8 (CCK-8) is designed to detect cell proliferation and cell toxicity.<\/span><\/p>\n

CCK-8; WST-8; CCK8; WST8<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCell Counting Kit-8 (CCK-8)<\/a><\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nCCK-8; WST-8; CCK8; WST8<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLiquid solution<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 CCK-8 solution<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 One-bottle, ready-to-use solution.
\n\u2022 The product (formazan) produced by WST-8 is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe.
\n\u2022 The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
\n\u2022 The same cells can be used for other cell assays because of the low toxicity of CCK-8.<\/td>\n<\/tr>\n
Product name<\/strong><\/td>\nCell Counting Kit-8 (CCK-8)<\/a><\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nCCK-8; WST-8; CCK8; WST8<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLiquid solution<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 CCK-8 solution<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 One-bottle, ready-to-use solution.
\n\u2022 The product (formazan) produced by WST-8 is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe.
\n\u2022 The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
\n\u2022 The same cells can be used for other cell assays because of the low toxicity of CCK-8.<\/td>\n<\/tr>\n
ackground<\/strong><\/td>\nCell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8.[2-(2- methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. The product (formazan) produced by WST-8 is water soluble, no organic solvents or isotopes required. And the formazan is stable and safe. The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nCCK-8; WST-8; CCK8; WST8<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
<\/div>\n

\nCell Number Determination Protocol<\/span><\/strong><\/span><\/div>\n
\n
\n
\n

1. \u5c07\u7d30\u80de\u61f8\u6d6e\u6db2\uff08100 \u03bcl\/\u5b54\uff09\u63a5\u7a2e\u65bc 96 \u5b54\u677f\u4e2d\u3002\u5c07\u677f\u5728\u52a0\u6fd5\u57f9\u990a\u7bb1\u4e2d\u9810\u5148\u57f9\u990a\uff08\u4f8b\u5982\uff0c\u5728 37\u00b0C\u30015% \u4e8c\u6c27\u5316\u78b3\uff09\u3002
\n2. \u5c07 10 \u03bcl CCK-8 \u6eb6\u6db2<\/span>\u52a0\u5165\u677f\u7684\u6bcf\u500b\u5b54\u4e2d\u3002\u5c0f\u5fc3\u4e0d\u8981\u5411\u5b54\u4e2d\u5f15\u5165\u6c23\u6ce1\uff0c\u56e0\u70ba\u5b83\u5011\u5e72\u64fe OD \u8b80\u6578\u3002
\n3. \u5c07\u677f\u5728\u57f9\u990a\u7bb1\u4e2d\u57f9\u990a 1 – 4 \u5c0f\u6642\u3002\u5b75\u80b2\u6642\u9593\u53d6\u6c7a\u65bc\u5be6\u9a57\u689d\u4ef6\uff0c\u4f8b\u5982\u7d30\u80de\u985e\u578b\u548c\u7d30\u80de\u5bc6\u5ea6\uff1b
\n4. \u4f7f\u7528\u9176\u6a19\u5100\u6e2c\u91cf 450 nm \u8655\u7684\u5438\u5149\u5ea6\u3002\u70ba\u4e86\u7a0d\u5f8c\u6e2c\u91cf\u5438\u5149\u5ea6\uff0c\u5728\u6bcf\u500b\u5b54\u4e2d\u52a0\u5165 10 \u03bcl 1% w\/v SDS \u6216 0.1 M HCl\uff0c\u84cb\u4e0a\u677f\u4e26\u7528\u5ba4\u6eab\u4e0b\u907f\u5149\u3002 24 \u5c0f\u6642\u5167\u4e0d\u61c9\u89c0\u5bdf\u5230\u5438\u5149\u5ea6\u8b8a\u5316<\/p>\n

\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a(Cell Proliferation and Cytotoxicity Assay Protocol)<\/span><\/strong><\/span><\/p>\n

1. \u5c07 100 \u03bcl \u7d30\u80de\u61f8\u6d6e\u6db2\uff085000 \u500b\u7d30\u80de\/\u5b54<\/span>\uff09<\/span>\u5206\u88dd\u5230 96 \u5b54\u677f\u4e2d\u3002\u5c07\u677f\u5728\u6fd5\u5316\u74b0\u5883\u4e0b\u9810\u5b75\u80b2 24 \u5c0f\u6642\u57f9\u990a\u7bb1\uff08\u4f8b\u5982\uff0c37\u00b0C\uff0c5% CO2\uff09\u3002
\n2. \u5c071-10\u03bcl<\/span><\/span>\u4e0d\u540c\u6fc3\u5ea6\u7684\u5f85\u6e2c\u7269\u8cea\u52a0\u5165\u677f\u4e2d\u3002
\n3. \u5c07\u677f\u5728\u57f9\u990a\u7bb1\u4e2d\u57f9\u990a\u9069\u7576\u7684\u6642\u9593\uff08\u4f8b\u5982\uff0c6\u300112\u300124 \u6216 48 \u5c0f\u6642\uff09\u3002
\n4. \u5728\u677f\u7684\u6bcf\u500b\u5b54\u4e2d\u52a0\u5165 10 \u03bcl CCK-8<\/span> <\/span>\u6eb6\u6db2\u3002\u5c0f\u5fc3\u4e0d\u8981\u5c07\u6c23\u6ce1\u5f15\u5165\u5b54\u4e2d\uff0c\u56e0\u70ba\u5b83\u5011\u6703\u5e72\u64fe\u8207 OD \u8b80\u6578\u3002
\n5. \u5c07\u677f\u5728\u57f9\u990a\u7bb1\u4e2d\u57f9\u990a 1 – 4 \u5c0f\u6642\u3002\u5b75\u80b2\u6642\u9593\u53d6\u6c7a\u65bc\u5be6\u9a57\u689d\u4ef6\uff0c\u4f8b\u5982\u7d30\u80de\u985e\u578b\u548c\u7d30\u80de\u5bc6\u5ea6\uff1b
\n6. \u4f7f\u7528\u9176\u6a19\u5100\u6e2c\u91cf 450 nm \u8655\u7684\u5438\u5149\u5ea6\u3002\u70ba\u4e86\u7a0d\u5f8c\u6e2c\u91cf\u5438\u5149\u5ea6\uff0c\u5728\u6bcf\u500b\u5b54\u4e2d\u52a0\u5165 10 \u03bcl 1% w\/v SDS \u6216 0.1 M HCl\uff0c\u84cb\u4e0a\u677f\u4e26\u7528\u5ba4\u6eab\u4e0b\u907f\u5149\u3002 24 \u5c0f\u6642\u5167\u4e0d\u61c9\u89c0\u5bdf\u5230\u5438\u5149\u5ea6\u8b8a\u5316<\/p>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e5\u0084\u0080\u00e5\u0099\u00a8\"\u5546\u54c1\u9700\u7528\u5100\u5668\u6e2c\u91cf<\/span>\u00a0\u00a0\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100\u8acb\u6309\u6b64\u00a0<\/a><\/span>(\u6a19\u914d405\u3001450\u3001492\u3001630 nm, \u53ef\u64f4\u589e\u81f37\u500b\u6ffe\u7247) \u6216\u4ee5\u5206\u5149\u5149\u5ea6\u8a08\u6e2c\u91cf (\u5fae\u91cfCuvette)<\/p>\n

\"RT-6900\"<\/a><\/p>\n

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 <\/p>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2\u00a0|\u00a0\u8ca8\u865f\u00a0KTA1020\u00a0\u00a0\u00a0\u5438\u6536\u5149450 nm\u00a0\u00a0\u00a0\u00a0CCK8\u64cd\u4f5c\u624b\u518a\u00a0 […]<\/p>\n","protected":false},"author":3,"featured_media":15881,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1401],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25867"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25867"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25867\/revisions"}],"predecessor-version":[{"id":46422,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25867\/revisions\/46422"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/15881"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25867"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25867"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25867"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}