{"id":25863,"date":"2020-09-02T12:54:39","date_gmt":"2020-09-02T04:54:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25863"},"modified":"2023-09-11T14:47:40","modified_gmt":"2023-09-11T06:47:40","slug":"chekine%e4%b9%b3%e9%85%b8%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-chekine-lactate-assay-kit%e8%b2%a8%e8%99%9fktb1100","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25863","title":{"rendered":"CheKine\u2122\u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Lactate Assay Kit\u8ca8\u865fKTB1100"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=25863\">CheKine\u2122\u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2 <strong>|<\/strong> <strong>CheKine\u2122 Lactate Assay Kit<\/strong><strong>\u8ca8\u865f<\/strong>KTB1100<\/a><\/span><\/p>\n<p>\u5075\u6e2c\u7bc4\u570d:\u00a0<strong><u>0.03 mM-2 mM<\/u><\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%8E%9F%E7%90%86%E4%BB%8B%E7%B4%B9-300x114.png\" alt=\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\" width=\"93\" height=\"35\" \/>\u4e73\u9178\uff08CH 3 CH\uff08OH\uff09COO-\uff09\u5728\u8a31\u591a\u751f\u7269\u904e\u7a0b\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002\u5728\u589e\u6b96\u7d30\u80de\u4e2d\u7522\u751f\u7121\u6c27\u904b\u52d5\u7b49\u689d\u4ef6\u3002\u7570\u5e38\u9ad8\u6fc3\u5ea6\u7684\u4e73\u9178(Lactate )\u8207\u8af8\u5982\u764c\u75c7\u3001\u7cd6\u5c3f\u75c5(\u00a0diabetes )\u548c\u4e73\u9178\u9178\u4e2d\u6bd2(lactate acidosis)\u7b49\u75c5\u7406\u72c0\u6cc1\u6709\u95dc\u3002L\uff08+\uff09 &#8211; \u4e73\u9178\u9e7d(L(+)-Lactate)\u662f\u5728\u4eba\u985e\u4e2d\u9593\u4ee3\u8b1d\u4e2d\u5f62\u6210\u7684\u4e73\u9178\u9e7d\u4e4b\u4e3b\u8981\u7acb\u9ad4\u7570\u69cb\u9ad4 (stereo-isomer )\u3002\uff0c\u4e26\u4e14\u5728\u8840\u6db2\u4e2d\u7684\u542b\u91cf\u7d04\u70ba1-2 mmol \/ L\u3002 D\uff08-\uff09-\u4e73\u9178\u4e5f\u53ef\u4ee5\u5728\u8840\u6db2\uff0c\u4f46\u50c5\u7d04\u4f54L\uff08+\uff09-\u4e73\u9178\u6fc3\u5ea6\u76841-5\uff05\u3002\u64cd\u4f5c\u624b\u518a\u00a0<a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/04\/CheKine\u2122-\u4e73\u9178\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2-CheKine\u2122-Lactate-Colorimetric-Assay-Kit\u8ca8\u865fKTB1100.pdf\">CheKine\u2122 Lactate Assay Kit<\/a><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abbkine.com\/file\/img\/KTB1100.png\" alt=\"\" \/><\/p>\n<p>Fig. Standard Curve of Lactate assay. The y-axis is OD450 and the x-axis is Lactate concentration (mM).<\/p>\n<p><span style=\"color: #800000;\"><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong><\/span>CheKine\u2122\u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2(CheKine\u2122 Lactate Assay Kit, KTB1100)\u70ba\u6aa2\u6e2c\u751f\u7269\u6a23\u54c1\uff08\u4f8b\u5982\u8840\u6e05\u6216\u8840\u6f3f\uff0c\u7d30\u80de\uff0c\u57f9\u990a\u57fa\u548c\u767c\u9175\u57f9\u990a\u57fa\uff09\u4e2d\u7684L-\uff08+\uff09-\u4e73\u9178\u63d0\u4f9b\u4e86\u4fbf\u5229\u7684\u65b9\u6cd5\u3002\u5728\u8a72\u8a66\u5291\u76d2\u4e2d\uff0c\u4e73\u9178\u88ab\u4e73\u9178\u812b\u6c2b\u9176(lactate dehydrogenase)\u6c27\u5316\uff0c\u751f\u6210\u8207\u56db\u5511\u9e7d(tetrazolium salt , WST-8)\u67d3\u6599\u76f8\u4e92\u4f5c\u7528\u7684\u7522\u7269\uff0c\u5f62\u6210\u6bd4\u8272\uff08450 nm\uff09\u7522\u7269\uff0c\u8207\u5b58\u5728\u7684\u4e73\u9178(lactate)\u6210\u6bd4\u4f8b\u3002\u5075\u6e2c\u7bc4\u570d: <strong><u>0.03 mM-2 mM<\/u><\/strong>\u00a0<strong>\u3002<\/strong>\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: serum or plasma, cells, culture and fermentation media<\/p>\n<p><strong><span style=\"color: #800000;\">\u6ce8\u610f\u4e8b\u9805: <\/span><\/strong>\u7d30\u80de\u6216\u7d44\u7e54\u63d0\u53d6\u7269\u4e2d\u7684NADH\u6216NADPH\u7522\u751f\u4e73\u9178\u7684\u80cc\u666f\u5206\u6790\u3002\u8981\u53bb\u9664NADH\u6216NADPH\u80cc\u666f\uff0c\u53ef\u4ee5\u5c07\u76f8\u540c\u91cf\u7684\u6a23\u54c1\u5728\u6c92\u6709\u4e73\u9178\u812b\u6c2b\u9176\u7684\u689d\u4ef6\u4e0b\u9032\u884c\u6e2c\u8a66\u3002\u7136\u5f8c\u53ef\u4ee5\u5c07\u80cc\u666f\u8b80\u6578\u5f9e\u4e73\u9178\u8b80\u6578\u4e2d\u6e1b\u53bb\u3002\u5167\u6e90\u6027\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff09\u53ef\u80fd\u964d\u89e3\u4e73\u9178\u3002\u6a23\u54c1\u542bLDH\uff08\u4f8b\u5982\u57f9\u990a\u57fa\u6216\u7d44\u7e54\u88c2\u89e3\u6db2\uff09\u61c9\u901a\u904e10 KDa MW\u904e\u6ffe\u65cb\u8f49\u904e\u6ffe\u5668\u9664\u53bb\u6240\u6709\u86cb\u767d\u8cea\uff0c\u7136\u5f8c\u4fdd\u6301\u5728-80\u00b0C\u9032\u884c\u5132\u5b58\u3002<\/p>\n<ul>\n<li><strong>Product name<\/strong><\/li>\n<\/ul>\n<p>CheKine\u2122 Lactate Assay Kit<\/p>\n<ul>\n<li><strong>Application notes<\/strong><\/li>\n<\/ul>\n<p>CheKine\u2122 Lactate Assay Kit provides a convenient means for detecting L-(+)-Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. In this kit lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a tetrazolium salt WST-8 dye to form a colorimetric (450 nm) product, proportional to the lactate present.<\/p>\n<ul>\n<li><strong>Kit components<\/strong><\/li>\n<li>Lactate Assay Buffer<br \/>\n\u2022 Lactate Dehydrogenase<br \/>\n\u2022 Lactate Dehydrogenase Cofactor<br \/>\n\u2022 WST-8<br \/>\n\u2022 Enhancer<br \/>\n\u2022 L(+)-Lactate Standard (100 mM)<\/li>\n<li><strong>Features &amp; Benefits<\/strong><\/li>\n<li>Determination of L-(+)-Lactate in serum, plasma, cell\/tissue samples, urine and other biological fluids.<br \/>\n\u2022 Detect biological samples at a level of 0.03 mM-2 mM.<br \/>\n\u2022 Pre-treatment or purification of samples are not required.<br \/>\n\u2022 Using WST-8 as substrate, more sensitive than MTT.<\/li>\n<li><strong>Usage notes<\/strong><\/li>\n<li>If not assayed immediately, samples can be stored at -80\u00b0C for one month.<br \/>\n\u2022 It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.<br \/>\n\u2022 Fresh samples are necessary for good results. If don&#8217;t perform the assay at the same time, it&#8217;s better to complete the Sample Preparation step before storing the samples.<\/li>\n<li><strong>Storage instructions<\/strong><\/li>\n<\/ul>\n<p>Storage at -20\u00b0C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.<\/p>\n<ul>\n<li><strong>Shipping<\/strong><\/li>\n<\/ul>\n<p>Gel pack with blue ice.<\/p>\n<p>\u5f15\u7528\u6587\u737b<\/p>\n<p><a href=\"http:\/\/www.ajtr.org\/files\/ajtr0096507.pdf\">Experimental evidence and network pharmacology identify the molecular targets of Tong Sheng tablets in cerebral ischemia reperfusion injury<\/a><\/p>\n<p>Tuo Liu, Kangqiang Yang, Guoshun Li, et al. Am J Transl Res 2019;11(6):3301-3316.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CheKine\u2122\u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Lactate Assay Kit\u8ca8\u865fKTB1100 \u5075 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":25491,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1402],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25863"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25863"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25863\/revisions"}],"predecessor-version":[{"id":45747,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25863\/revisions\/45747"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/25491"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25863"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25863"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25863"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}