{"id":25707,"date":"2020-08-11T12:23:12","date_gmt":"2020-08-11T04:23:12","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25707"},"modified":"2022-07-19T16:49:14","modified_gmt":"2022-07-19T08:49:14","slug":"his-tag%e6%a8%99%e7%b1%a4%e5%a4%9a%e6%a0%aa%e6%8a%97%e9%ab%94-hrp-%e8%b2%a8%e8%99%9f-a02051hrp","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25707","title":{"rendered":"His-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP  | \u8ca8\u865f A02051HRP"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=25704\">His-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0\u00a0<strong>|\u00a0<\/strong><strong>\u8ca8\u865f\u00a0A02051HRP<\/strong><\/a><\/span><\/p>\n<p>Anti-His Tag Rabbit Polyclonal Antibody, HRP Conjugated<\/p>\n<p><strong>His-tag<\/strong><strong>\u6a19\u7c64\u4ecb\u7d39<\/strong><strong>:\u00a0<\/strong>His-tag\u591a\u7d44\u6c28\u9178\u6a19\u7c64(polyhistidine-tag)\u662f\u86cb\u767d\u8cea\u4e2d\u7684\u6c28\u57fa\u9178\u57fa\u5e8f(histidine (His) residues)\uff0c\u901a\u5e38\u7531\u86cb\u767d\u8cea\u7684N\u6216C\u7aef\u7d44\u6210\uff0c\u7531\u81f3\u5c11\u4e94\u500b\u7d44\u6c28\u9178\uff08His\uff09\u6b98\u57fa\u7d44\u6210\u3002\u5b83\u4e5f\u88ab\u7a31\u70ba\u516d\u7d44\u6c28\u9178\u6a19\u7c64(6xHis-tag)\uff0c6xHis\u6a19\u7c64\u548c\u5546\u6a19\u540d\u7a31His-tag\u3002\u591a\u7d44\u6c28\u9178\u6a19\u7c64(Polyhistidine-tags)\u901a\u5e38\u7528\u65bc\u89aa\u548c\u7d14\u5316\u5728\u5927\u8178\u687f\u83cc(Escherichia coli)\u548c\u5176\u4ed6\u539f\u6838\u8868\u9054\u7cfb\u7d71(prokaryotic expression)\u4e2d\u8868\u9054\u7684\u591a\u7d44\u6c28\u9178\u6a19\u7c64\u7684\u91cd\u7d44\u86cb\u767d(recombinant proteins)\u3002A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.<\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<\/strong>\u00a0His-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4 (\u5177HRP\u6a19\u8a8c) \u3002Abbkine\u63d0\u4f9b\u4e00\u7cfb\u5217Tag\u55ae\u591a\u682a\u6297\u9ad4\uff0c\u6297His\u6a19\u7c64\u5154\u591a\u682a\u6297\u9ad4\uff0c\u514d\u75ab\u539f\u70ba\u5408\u6210\u80dc\u80bd(Synthetic Peptide)\u3002\u7d93\u7531Rabbit\u514d\u75ab\u5f8c, \u6297\u9ad4\u7d14\u5316\u4e26\u4fdd\u5b58\u65bcLiquid in Liquid in PBS, pH 7.4 and 50% Glycerol. \u6297His\u6a19\u7c64\u5154\u591a\u682a\u6297\u9ad4-HRP, \u53ef\u61c9\u7528\u65bcWestern blot\u5be6\u9a57(1:5000)\u3002<\/p>\n<ul class=\"product-info\">\n<li><span class=\"info-name\">Product name<\/span>Anti-His Tag Rabbit Polyclonal Antibody, HRP Conjugated<\/li>\n<li><span class=\"info-name\">Immunogen:\u00a0<\/span>Synthetic Peptide<\/li>\n<li><span class=\"info-name\">Host:\u00a0<\/span>Rabbit<\/li>\n<li><span class=\"info-name\">Applications:\u00a0<\/span>WB<\/li>\n<li><span class=\"info-name\">Application notes:\u00a0<\/span>Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).<\/li>\n<li><span class=\"info-name\">Clonality:\u00a0<\/span>Polyclonal<\/li>\n<li><span class=\"info-name\">Isotype:\u00a0<\/span>Rabbit IgG<\/li>\n<li><span class=\"info-name\">Purification<\/span>The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen<\/li>\n<li>Conjugate:\u00a0 HRP<\/li>\n<\/ul>\n<ul class=\"product-info\">\n<li><span class=\"info-name\">Formulation:\u00a0<\/span>Liquid solution<\/li>\n<li><span class=\"info-name\">Storage buffer:\u00a0<\/span>Liquid in PBS, pH 7.4 and 50% Glycerol.<\/li>\n<li><span class=\"info-name\">Storage instructions:\u00a0<\/span>Stable for one year at -20\u00b0C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>His-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0\u00a0|\u00a0\u8ca8\u865f\u00a0A02051HRP Anti-His Tag Rabbit P [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":25491,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136,3],"tags":[1421],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25707"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25707"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25707\/revisions"}],"predecessor-version":[{"id":25711,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25707\/revisions\/25711"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/25491"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25707"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25707"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25707"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}