{"id":25697,"date":"2020-08-05T19:36:44","date_gmt":"2020-08-05T11:36:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25697"},"modified":"2022-07-19T16:49:48","modified_gmt":"2022-07-19T08:49:48","slug":"mouse-anti-human-cd45-cy5%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f9625-15-%e8%a6%8f%e6%a0%bc100test","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25697","title":{"rendered":"Mouse Anti-Human CD45-CY5\u6297\u9ad4 | \u8ca8\u865f9625-15 \u898f\u683c100Test"},"content":{"rendered":"

Mouse Anti-Human CD45-CY5\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>9625-15 \u00a0<\/strong>\u898f\u683c<\/strong>100Test<\/a><\/span><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

CD45\uff0c\u4e5f\u7a31\u70ba\u767d\u7d30\u80de\u5171\u540c\u6297\u539f\uff08LCA\uff09\uff0c\u4ee5\u56db\u7a2e\u4e0d\u540c\u7684\u540c\u5de5\u578b\uff08180\u3001190\u3001205\u3001220 kDa\uff09\u5b58\u5728\uff0c\u5b83\u5011\u662f\u901a\u904e\u4e09\u500b\u5916\u986f\u5b50\u7684\u53ef\u8b8a\u526a\u63a5\u7522\u751f\u7684\u3002 LCA\u5b58\u5728\u65bc\u6240\u6709\u9020\u8840\u7d30\u80de\u4e0a\uff0c\u4f46\u7d05\u7d30\u80de\u548c\u8840\u5c0f\u677f\u9664\u5916\u3002 CD45\u8868\u9054\u662fT\u7d30\u80de\u548cB\u7d30\u80de\u6297\u539f\u53d7\u9ad4\u4ecb\u5c0e\u7684\u6fc0\u6d3b\u6240\u5fc5\u9700\u7684\u3002<\/p>\n

\"\"<\/p>\n

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45-CY5 (SB Cat. No. 9625-15).<\/p>\n

<\/div>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0Mouse Anti-Human CD45-CY5\u6297\u9ad4\uff0c\u5177HRP\u6a19\u8a8c\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nF10-89-4<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse IgG2a<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG2a<\/sub>-CY5<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nPurified T cells from human lymph nodes<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nHuman\/Horse CD45<\/td>\n<\/tr>\n
Alternalte Name(s)<\/strong><\/td>\nLCA, T200, Ly5<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nCD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation.<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nCY5 (Cyanine 5)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nPhosphate buffered saline containing 0.1% sodium azide<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C; Avoid exposure to light
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

Flow Cytometry \u2013 Quality tested\u00a01,5,9-11<\/sup>
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a01-3<\/sup>
\nImmunocytochemistry \u2013 Reported in literature\u00a04,5<\/sup>
\nImmunoprecipitation \u2013 Reported in literature\u00a06,7<\/sup>
\nWestern Blot \u2013 Reported in literature\u00a07,8<\/sup><\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2797030<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n
Gene ID<\/strong><\/td>\n\n

5788<\/a>\u00a0(Human)<\/p>\n

100061950<\/a>\u00a0(Horse)<\/td>\n<\/tr>\n

Gene Symbol<\/strong><\/td>\n\n

PTPRC<\/a>\u00a0(Human)<\/p>\n

PTPRC<\/a>\u00a0(Horse)<\/td>\n<\/tr>\n

Gene Aliases<\/strong><\/td>\nB220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200<\/td>\n<\/tr>\n
Uniprot ID<\/strong><\/td>\nP08575<\/a>\u00a0(Human)<\/td>\n<\/tr>\n
Uniprot Name<\/strong><\/td>\nPTPRC_HUMAN<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/p>\n

    \n
  1. Dalchau R, Kirkley J, Fabre JW. Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Eur J Immunol. 1980;10:737-44. (Immunogen, FC, IHC-FS)<\/li>\n
  2. Mason DY, Cordell JL, Abdulaziz Z, Naiem M, Bordenave G. Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies. J Histochem Cytochem. 1982;30:1114-22. (IHC-FS)<\/li>\n
  3. Salter DM, Krajewski AS, Dewar AE. Immunophenotype analysis of malignant histiocytosis of the intestine. J Clin Pathol. 1986;39:8-15. (IHC-FS)<\/li>\n
  4. Toscano MA, Bianco GA, Ilarregui JM, Croci DO, Correale J, Hernandez JD, et al. Differential glycosylation of TH1, TH2 and TH-17 effector cells selectively regulates susceptibility to cell death. Nat Immunol. 2007;8:825-34. (ICC)<\/li>\n
  5. De Schauwer C, Piepers S, Van de Walle GR, Demeyere K, Hoogewijs MK, Govaere JL, et al. In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry. Cytometry A. 2012;81:312-23. (FC, ICC, Horse Reactivity)<\/li>\n
  6. Chen I, Chen H, Demetriou M. Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling. J Biol Chem. 2007;282:35361-72. (IP)<\/li>\n
  7. Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I. Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45\/B220 on the surface of human B lymphocytes. J Immunol. 2004;172:4298-307. (IP, WB)<\/li>\n
  8. Stillman BN, Hsu DK, Pang M, Brewer CF, Johnson P, Liu F, et al. Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. J Immunol. 2006;176:778-89. (WB)<\/li>\n
  9. Young AJ, Holzgreve W, Dudler L, Schoeberlein A, Surbeck DV. Engraftment of human cord blood-derived stem cells in preimmune ovine fetuses after ultrasound-guided in utero transplantation. Am J Obstet Gynecol. 2003;189:698-701. (FC)<\/li>\n
  10. Mitchell P, Lee F, Hall C, Rigopoulos A, Smyth FE, Hekman A, et al. Targeting primary human Ph+ B-cell precursor leukemia-engrafted SCID mice using radiolabeled anti-CD19 monoclonal antibodies. J Nucl Med. 2003;44:1105-12. (FC)<\/li>\n
  11. R\u00f8sland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, et al. Long-term cultures of bone marrow-derived<\/li>\n<\/ol>\n

    Cy\u00ae is a registered trademark of GE Healthcare<\/p>\n

    \u00a0<\/strong>SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    Mouse Anti-Human CD45-CY5\u6297\u9ad4 | \u8ca8\u865f9625-15 \u00a0\u898f\u683c100Test \u7f8e\u570b\u6297\u9ad4 […]<\/p>\n","protected":false},"author":3,"featured_media":6494,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1425],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25697"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25697"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25697\/revisions"}],"predecessor-version":[{"id":25701,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25697\/revisions\/25701"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6494"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25697"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25697"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25697"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}