{"id":25691,"date":"2020-08-03T18:20:56","date_gmt":"2020-08-03T10:20:56","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25691"},"modified":"2024-03-01T15:02:15","modified_gmt":"2024-03-01T07:02:15","slug":"%e7%a9%80%e8%83%b1%e7%94%98%e8%82%bdglutathione%e6%aa%a2%e6%b8%ac-chekine-reduced-glutathione-gsh-colorimetric-assay-kit%e8%b2%a8%e8%99%9fktb1600","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25691","title":{"rendered":"\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c |  CheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit\u8ca8\u865fKTB1600"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=25691\"><span style=\"color: #33cccc;\">\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c <strong>| \u00a0<\/strong>CheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit<strong>\u8ca8\u865f<\/strong>KTB1600\u00a0\u00a0\u5075\u6e2c\u9748\u654f\u5ea62-200 \u00b5g\/mL.\u00a0\u00a0<\/span><\/a><\/span><\/p>\n<p>\u7a40\u80f1\u7518\u80bd (Glutathione) \u7814\u7a76\u7684\u610f\u7fa9<\/p>\n<p>\u7a40\u80f1\u7518\u80bd\u662f\u542b\u6709\u8c37\u6c28\u9178 (glutamate)\uff0c\u534a\u80f1\u6c28\u9178(cysteine)\u548c\u7518\u6c28\u9178(glycine)\u7684\u4e09\u80bd\uff0c\u5176\u4e2d\u542b\u6709\u5df0\u57fa(sulfhydryl groups)\u3002\u5b83\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u690d\u7269\u4e2d\u3002\u5b83\u5177\u6709\u6297\u6c27\u5316\u5291(antioxidant)\u548c\u7d9c\u5408\u89e3\u6bd2(detoxification)\u4f5c\u7528\u3002\u5176\u76f8\u95dc\u7684\u8f14\u9176(coenzyme)\u662fNADPH\u3002\u4eba\u9ad4\u65b0\u9673\u4ee3\u8b1d\u7522\u751f\u7684\u81ea\u7531\u57fa(free radicals)\u904e\u591a\u6703\u7834\u58de\u751f\u7269\u819c(biofilm)\uff0c\u4fb5\u5165\u751f\u547d\u5927\u5206\u5b50\uff0c\u52a0\u901f\u4eba\u9ad4\u8870\u8001(aging)\uff0c\u4e26\u8a98\u767c\u816b\u7624(tumors)\u6216\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis)\u7684\u7522\u751f\u3002\u7a40\u80f1\u7518\u80bd\u5177\u6709\u589e\u5f37\u751f\u7269\u9ad4\u7684\u6297\u6c27\u5316\u529f\u80fd\uff0c\u4fc3\u9032\u751f\u9577\u4ee5\u53ca\u589e\u5f37\u751f\u7269\u9ad4\u5c0d\u4eba\u9ad4\u4e0d\u5229\u74b0\u5883\u7684\u5f71\u97ff\u7684\u529f\u80fd\u3002\u5b83\u7684\u4e3b\u8981\u751f\u7406\u4f5c\u7528\u662f\u6e05\u9664\u9ad4\u5167\u7684\u81ea\u7531\u57fa\u3002\u5b83\u4e5f\u662f\u4e00\u7a2e\u5f88\u597d\u7684\u89e3\u6bd2\u5291\uff0c\u53ef\u4ee5\u6e1b\u5f31\u6709\u6bd2\u7269\u8cea\u7684\u6bd2\u6027\u4e26\u5f9e\u9ad4\u5167\u6e05\u9664\u6709\u6bd2\u7269\u8cea\u3002\u7a40\u80f1\u7518\u80bd\u9084\u4fc3\u9032\u751f\u9577\u4e26\u63d0\u9ad8\u4eba\u9ad4\u514d\u75ab\u529b\u3002\u7a40\u80f1\u7518\u80bd(Glutathione)\u4e0d\u50c5\u53ef\u4ee5\u7528\u4f5c\u85e5\u7269\uff0c\u9084\u53ef\u4ee5\u7528\u4f5c\u529f\u80fd\u6027\u98df\u54c1\u7684\u57fa\u790e\u6750\u6599\uff0c\u4e26\u4e14\u5ee3\u6cdb\u7528\u65bc\u529f\u80fd\u6027\u98df\u54c1\u4e2d\uff0c\u4f8b\u5982\u6297\u8870\u8001(anti-aging) \uff0c\u589e\u5f37\u514d\u75ab\u529b(enhancing immunity)\u548c\u6297\u816b\u7624\u4f5c\u7528(anti-tumor)\u3002<\/p>\n<p>\u7a40\u80f1\u7518\u80bd\u6709\u5169\u7a2e\u5f62\u5f0f\uff1a\u9084\u539f\u578b\uff08GSH\uff09\u548c\u6c27\u5316\u578b\uff08GSSG\uff09\u3002\u6c27\u5316\u7a40\u80f1\u7518\u80bd(Glutathione oxidized)\uff0c\u4e5f\u7a31\u70ba\u4e8c\u786b\u4ee3\u7a40\u80f1\u7518\u80bd(Dithioglutathione)\uff0c\u5176\u662f\u901a\u904e\u5169\u500b\u9084\u539f\u7684\u7a40\u80f1\u7518\u80bd\u5206\u5b50\u7684\u6c27\u5316\u5f62\u6210\u7684\u3002\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u5c07GSSG\u9084\u539f\u70baGSH\u3002\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u5b58\u5728\u65bc\u5927\u91cf\u751f\u7269\u4e2d\uff0c\u4e26\u8d77\u4e3b\u8981\u4f5c\u7528\u3002\u5728\u7d05\u8840\u7403\u7d30\u80de\u4e2d\uff0c\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u662f\u4fdd\u6301\u8840\u7d05\u86cb\u767d\u8655\u65bc\u9084\u539f\u72c0\u614b\u4e26\u4fdd\u8b77\u7d30\u80de\u514d\u53d7\u6c27\u5316\u640d\u50b7\u7684\u95dc\u9375\u3002 GSH\u662f\u7d30\u80de\u4e2d\u6700\u91cd\u8981\u7684\u6297\u6c27\u5316\u5291\u5df0\u57fa\u5316\u5408\u7269\uff0c\u5728\u6297\u6c27\u5316\u6027\uff0c\u86cb\u767d\u8cea\u5df0\u57fa\u4fdd\u8b77\u548c\u6c28\u57fa\u9178\u8de8\u819c\u904b\u8f38\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002\u9084\u539f\u578b\u548c\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\u4e4b\u9593\u7684\u6bd4\u4f8b\uff08GSH \/ GSSG\uff09\u662f\u7d30\u80de\u6c27\u5316\u9084\u539f\u72c0\u614b\u7684\u4e3b\u8981\u6307\u6a19\u3002\u56e0\u6b64\uff0c\u6e2c\u91cf\u7d30\u80de\u4e2dGSH\u548cGSSG\u7684\u542b\u91cf\u4ee5\u53caGSH \/ GSSG\u7684\u6bd4\u503c\u53ef\u4ee5\u53cd\u6620\u7d30\u80de\u7684\u6c27\u5316\u9084\u539f\u72c0\u614b\uff0c\u4f46\u662f\u5176\u4ed6\u5177\u6709\u904e\u6c27\u5316\u6d3b\u6027\u7684\u9176\u537b\u6c92\u6709\u5229\u7528\u9019\u4e9b\u5e95\u7269\u3002\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c (\u8ca8\u865fKTB1600) \u5957\u7d44: \u539f\u7406DTNB\u8207\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53cd\u61c9\u5f62\u6210\u9ec3\u8272\u7522\u7269\u3002\u5728412 nm\u8655\u6e2c\u5f97\u7684\u5149\u5bc6\u5ea6\u53ef\u4ee5\u76f4\u63a5\u53cd\u6620\u6a23\u54c1\u4e2d\u7684\u7a40\u80f1\u7518\u80bd\u6fc3\u5ea6\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/12\/citation-5_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1.jpg\" width=\"297\" height=\"55\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/01\/%E7%A9%80%E8%83%B1%E7%94%98%E8%82%BDGlutathione%E6%AA%A2%E6%B8%AC-CheKine%E2%84%A2-Reduced-Glutathione-GSH-Colorimetric-Assay-Kit%E8%B2%A8%E8%99%9FKTB1600.png\" \/><\/p>\n<p>(<a href=\"https:\/\/www.hindawi.com\/journals\/omcl\/2023\/6935947\/fig3\/\">\u6587\u737b\u5f15\u7528<\/a>) Mep-S inhibits H<sub>2<\/sub>O<sub>2<\/sub>-induced ROS generation and decreases GSH content and SOD activity in SH-SY5Y cells. (a) Mep-S inhibited H<sub>2<\/sub>O<sub>2<\/sub>-induced intracellular ROS accumulation, as demonstrated by decreased DCFH-DA fluorescence intensity. Scale\u00a0\u2009<i>\u03bc<\/i>m. Mep-S inhibited the H<sub>2<\/sub>O<sub>2<\/sub>-induced decrease in GSH content (b) and SOD activity (c). The cells were pretreated with Mep-S (0.5-2\u2009<i>\u03bc<\/i>M) or NAC (1\u2009mM) for 1\u2009h prior to stimulation with H<sub>2<\/sub>O<sub>2<\/sub>\u00a0(400\u2009<i>\u03bc<\/i>M) for 2\u2009h (a) or 24\u2009h (b, c). Data are presented as the\u00a0\u00a0of 2-4 independent experiments.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/01\/3-%E7%A9%80%E8%83%B1%E7%94%98%E8%82%BDGlutathione%E6%AA%A2%E6%B8%AC-CheKine%E2%84%A2-Reduced-Glutathione-GSH-Colorimetric-Assay-Kit%E8%B2%A8%E8%99%9FKTB1600.jpg\" width=\"1041\" height=\"1280\" \/><\/p>\n<p>(<a href=\"https:\/\/www.hindawi.com\/journals\/omcl\/2023\/6935947\/fig3\/\">\u6587\u737b\u5f15\u7528<\/a>) Figure 7. (a\u2013d) Role of C. papaya on enzymatic antioxidants levels. p &lt; 0.05 was considered statistically significant among the groups: *-control; #-diabetes; $-C. papaya-treated T2DM.<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/05\/\u7a40\u80f1\u7518\u80bdGlutathione\u6aa2\u6e2c-CheKine\u2122-Reduced-Glutathione-GSH-Colorimetric-Assay-Kit\u8ca8\u865fKTB1600-\u5075\u6e2c\u9748\u654f\u5ea62-200-\u00b5g-mL..pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E6%93%8D%E4%BD%9C%E6%89%8B%E5%86%8A-300x144.png\" alt=\"\u64cd\u4f5c\u624b\u518a\" width=\"108\" height=\"53\" \/><\/a><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abbkine.com\/file\/img\/KTB1600.png\" alt=\"\" \/><\/p>\n<ul>\n<li><strong>Kit components<\/strong><\/li>\n<li>Extraction Buffer<br \/>\n\u2022 Assay Buffer<br \/>\n\u2022 Chromogen<br \/>\n\u2022 Standard<\/li>\n<li><strong>Features &amp; Benefits<\/strong><\/li>\n<li>Determination of Reduced Glutathione concentration in serum, plasma, tissue\/cell lysates and other biological fluids.<br \/>\n\u2022 A broad range linearity: 2-200 \u00b5g\/mL.<br \/>\n\u2022 Measure concentration down to 2 \u00b5g\/mL.<\/li>\n<li><strong>Usage notes<\/strong><\/li>\n<li>The sample can be kept at -80\u00b0C for 10 days. If not do experiments immediately.<br \/>\n\u2022 Always prepare fresh standards per use.<br \/>\n\u2022 Diluted standard solution is unstable and must be used within 4 hours.<\/li>\n<li><strong>Storage instructions<\/strong><\/li>\n<\/ul>\n<p>Storage at -20\u00b0C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.<\/p>\n<ul>\n<li><strong>Shipping<\/strong><\/li>\n<\/ul>\n<p>Gel pack with blue ice.<\/p>\n<table>\n<tbody>\n<tr>\n<td><strong>CAT#<\/strong><\/td>\n<td><strong>Product name<\/strong><\/td>\n<td><strong>classification<\/strong><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.abbkine.com\/product\/chekinetm-reduced-glutathione-gsh-colorimetric-assay-kit-ktb1600\/\">KTB1600<\/a><\/td>\n<td>CheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit<\/td>\n<td>Cell metabolism<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.abbkine.com\/product\/chekinetm-glutathione-oxidized-gssg-colorimetric-assay-kit-ktb1610\/\">KTB1610<\/a><\/td>\n<td>CheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit<\/td>\n<td>Cell metabolism<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.abbkine.com\/product\/chekinetm-glutathione-reductases-gr-colorimetric-assay-kit-ktb1620\/\">KTB1620<\/a><\/td>\n<td>CheKine\u2122 Glutathione Reductases (GR) Colorimetric Assay Kit<\/td>\n<td>Cell metabolism<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.abbkine.com\/product\/chekinetm-glutathione-s-transferase-gst-colorimetric-assay-kit-ktb1630\/\">KTB1630<\/a><\/td>\n<td>CheKine\u2122 Glutathione S-Transferase (GST) Colorimetric Assay Kit<\/td>\n<td>Cell metabolism<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.abbkine.com\/product\/chekinetm-glutathione-peroxidase-gsh-px-colorimetric-assay-kit-ktb1640\/\">KTB1640<\/a><\/td>\n<td>CheKine\u2122 Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit<\/td>\n<td>Cell metabolism<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.abbkine.com\/product\/chekinetm-thioredoxin-reductase-trxr-colorimetric-assay-kit-ktb1650\/\">KTB1650<\/a><\/td>\n<td>CheKine\u2122 Thioredoxin Reductase (TrxR) Colorimetric Assay Kit<\/td>\n<td>Cell metabolism<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/www.abbkine.com\/product\/chekinetm-thioredoxin-peroxidase-tpx-colorimetric-assay-kit-ktb1660\/\">KTB1660<\/a><\/td>\n<td>CheKine\u2122 Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit<\/td>\n<td>Cell metabolism<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?p=24999\">\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0<a href=\"http:\/\/biopioneer.com.tw\/?p=15880\"><strong><u>CCK-8<\/u><\/strong><strong><u>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0<a href=\"http:\/\/biopioneer.com.tw\/?p=25057\">LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0<a href=\"http:\/\/biopioneer.com.tw\/?p=25127\">\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0<a href=\"http:\/\/biopioneer.com.tw\/?p=25092\">\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>| <a href=\"http:\/\/biopioneer.com.tw\/?p=24762\">Abbkine\u00a0<\/a><a href=\"http:\/\/biopioneer.com.tw\/?p=24762\">\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>| <a href=\"http:\/\/biopioneer.com.tw\/?p=24764\">\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c | \u00a0CheKine\u2122 Reduced Glutathione (GS [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":24734,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1429],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25691"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25691"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25691\/revisions"}],"predecessor-version":[{"id":48867,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25691\/revisions\/48867"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24734"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25691"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25691"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25691"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}