{"id":25634,"date":"2020-07-10T13:18:16","date_gmt":"2020-07-10T05:18:16","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25634"},"modified":"2022-07-19T16:51:25","modified_gmt":"2022-07-19T08:51:25","slug":"%e6%a4%8d%e7%89%a9%e7%b5%84%e7%b9%94%e7%9b%b4%e6%8e%a5pcr%e5%a5%97%e7%b5%84-directpcr-%e8%b2%a8%e8%99%9fk296-50","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25634","title":{"rendered":"\u690d\u7269\u7d44\u7e54\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2 | \u8ca8\u865fK296-50"},"content":{"rendered":"

\u690d\u7269\u7d44\u7e54\u8403\u53d6\u5957\u7d44 | \u00a0\u8ca8\u865f<\/strong>K296-50<\/a><\/span><\/p>\n

Plant Tissue Extraction Kit<\/em><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39:\u00a0 Biovision\u7684\u690d\u7269\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2(Biovision\u2019s Plant Protein Extraction Kit , K296) \u63d0\u4f9b\u4e86\u5f9e\u5404\u7a2e\u4e7e\u71e5\u548c\u65b0\u9bae\u690d\u7269\u7d44\u7e54\u4e2d\u63d0\u53d6\u53ef\u6eb6\u6027\u86cb\u767d\u4ee5\u53ca\u5176\u4ed6\u751f\u7269\u5206\u5b50\u548c\u4ee3\u8b1d\u7522\u7269\u7684\u6709\u6548\u65b9\u6cd5\uff0c\u800c\u7121\u9700\u4f7f\u7528\u6db2\u6c2e(liquid nitrogen)\u3002\u8a72\u8a66\u5291\u76d2\u5305\u62ec\u7528\u65bc\u63d0\u53d6\u690d\u7269\u86cb\u767d\u548c\u4ee3\u8b1d\u7522\u7269\u7684\u512a\u5316\u63d0\u53d6\u7de9\u885d\u6db2 (Extraction Buffer )\uff0c\u7528\u65bc\u9632\u6b62\u86cb\u767d\u8cea\u964d\u89e3\u7684\u86cb\u767d\u9176\u6291\u5236\u5291\u6df7\u5408\u7269(Protease Inhibitor Cocktail )\u4ee5\u53ca\u7528\u65bc\u5747\u8cea\u5316\u690d\u7269\u7d44\u7e54\u7684\u5747\u8cea\u5316\u6775(Homogenization Pestles )\u548c\u8a66\u7ba1\u3002\u63d0\u53d6\u7684\u86cb\u767d\u8cea\u53ef\u7528\u65bc\u591a\u7a2e\u4e0b\u6e38\u61c9\u7528\uff0c\u4f8b\u5982\u86cb\u767d\u8cea\u5370\u8de1(\u00a0Western Blotting)\uff0c2-D\u51dd\u81a0\u96fb\u6cf3(2-D gel electrophoresis)\uff0c\u514d\u75ab\u6c89\u6fb1\u6e2c\u5b9a(\u00a0immunoprecipitation assay)\u548c\u9176\u6d3b\u6027\u6e2c\u5b9a(enzymatic activity assays )\u7b49\uff0c\u63d0\u53d6\u7684\u5c0f\u5206\u5b50\u53ef\u7528\u65bc\u690d\u7269\u4ee3\u8b1d\u7814\u7a76\u3002\u9019\u7a2e\u6613\u65bc\u4f7f\u7528\u548c\u5feb\u901f\u7684\u7a0b\u5e8f\uff0c\u53ef\u78ba\u4fdd\u59cb\u7d42\u5982\u4e00\u7684\u86cb\u767d\u8cea\u7522\u91cf\u301c2-9 mg \/ ml\uff0c\u5177\u9ad4\u53d6\u6c7a\u65bc\u6a23\u54c1\u985e\u578b\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK296-50<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n50 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nProtein Quantitation<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nExtraction of plant soluble protein and metabolites for various downstream applications such as Western Blotting, 2-D gel electrophoresis, immunoprecipitation assay, enzymatic activity assay and quantitation of metabolites etc.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple method for extracting soluble protein, biomolecules & metabolites from plant tissues without the use of liquid nitrogen
\n\u2022 Easy-to-use and rapid procedure ensures consistent protein yield ~ 2-9 mg\/ml depending on the sample type<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Plant Extraction Buffer
\n\u2022 Protease Inhibitor Cocktail (200X)
\n\u2022 Homogenization Pestles
\n\u2022 Homogenization Tubes (1.5 ml)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u690d\u7269\u7d44\u7e54\u8403\u53d6\u5957\u7d44 | \u00a0\u8ca8\u865fK296-50 Plant Tissue Extraction Kit \u7522\u54c1\u4ecb\u7d39: […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1437],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25634"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25634"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25634\/revisions"}],"predecessor-version":[{"id":25642,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25634\/revisions\/25642"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25634"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25634"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25634"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}