{"id":25627,"date":"2020-07-09T14:30:45","date_gmt":"2020-07-09T06:30:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25627"},"modified":"2023-04-11T16:44:28","modified_gmt":"2023-04-11T08:44:28","slug":"%e7%b4%b0%e8%8f%8cdna%e7%b4%94%e5%8c%96%e8%a9%a6%e5%8a%91%e7%9b%92-bacterial-dna-purification-kit%e8%b2%a8%e8%99%9fk1457","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25627","title":{"rendered":"\u7d30\u83ccDNA\u7d14\u5316\u8a66\u5291\u76d2 | Bacterial DNA Purification Kit\u8ca8\u865fK1457"},"content":{"rendered":"

\u7d30\u83ccDNA\u7d14\u5316\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u00a0<\/strong>\u8ca8\u865f<\/strong>K1457<\/strong><\/a><\/span><\/p>\n

Bacterial DNA Purification Kit, K1457<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u00a0<\/strong>BioVision\u7684\u7d30\u83ccDNA\u7d14\u5316\u8a66\u5291\u76d2II(Bacterial DNA Purification Kit, K1457) \u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\uff0c\u53ef\u9760\u7684\u65b9\u6cd5\uff0c\u53ef\u4f7f\u7528\u65cb\u8f49\u67f1(spin columns)\u5f9e\u591a\u7a2e\u7d30\u83cc\u4e2d\u5206\u96e2\u51fa\u9ad8\u8cea\u91cf\u7684total DNA\u3002\u8a72\u8a66\u5291\u76d2\u5c07\u5206\u96e2\u6240\u6709\u7d30\u80deDNA\uff0c\u5305\u62ecplasmid DNA\u3002\u4f7f\u7528\u65cb\u8f49\u67f1(spin columns)\u6700\u591a\u53ef\u8655\u74061×109<\/sup>\u500b\u7d30\u83cc\u7d30\u80de\u3002\u5728\u8a72\u8a66\u5291\u76d2\u4e2d\uff0c\u6536\u7a6b\u8655\u65bc\u5c0d\u6578\u671f\u7684\u7d30\u83cc\u7d30\u80de\uff0c\u4e26\u4f7f\u7528\u6eb6\u83cc\u9176(lysozyme)\u548c\u86cb\u767d\u9176K(Proteinase K.)\u6d88\u5316\u7d30\u83cc\u7d30\u80de\u58c1\u3002\u5c07\u88c2\u89e3\u7684\u6a23\u54c1\u4e0a\u6a23\u81f3\u96e2\u5fc3\u67f1\u3002DNA\u88ab\u6709\u6548\u5730\u7d50\u5408\u5230\u65cb\u8f49\u67f1\u4e0a\uff0c\u540c\u6642\u86cb\u767d\u8cea\u548c\u5176\u4ed6\u6c61\u67d3\u7269\u88ab\u53bb\u9664\u3002\u7136\u5f8c\u5c07\u7d14DNA\u5728\u7121\u83cc\u6c34\u6216\u6d17\u812b\u7de9\u885d\u6db2\u4e2d\u6d17\u812b\u3002\u6bcf\u500b\u96e2\u5fc3\u67f1\u53ef\u7d50\u5408\u7d04100\u03bcg\u57fa\u56e0\u7d44DNA (genomic DNA)\u3002\u4f7f\u7528\u8a72\u8a66\u5291\u76d2\u7d14\u5316\u7684DNA\u53ef\u7528\u65bc\u4e0b\u6e38\u61c9\u7528\uff0c\u4f8b\u5982PCR\uff0c\u9650\u5236\u6027\u9176\u5207\u548c\u96dc\u4ea4\u6280\u8853\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
Kit Summary<\/strong><\/td>\nHigh-quality DNA is purified from bacteria using spin columns<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nBacteria is lysed using lysozyme, glass beads and DNA is purified using spin columns.<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nPCR, restriction digestion, and hybridization<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Many downstream applications such as PCR, restriction digestion, and hybridization
\n\u2022 Rapid, easy and convenient
\n\u2022 Highly pure, high yield
\n\u2022 High quality spin columns<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Buffer TL
\n\u2022 Lysozyme
\n\u2022 Lysozyme Storage Buffer
\n\u2022 RNase A
\n\u2022 Glass Beads
\n\u2022 Proteinase K
\n\u2022 Buffer BL
\n\u2022 DNA Column
\n\u2022 Buffer KB
\n\u2022 *DNA Wash Buffer
\n\u2022 Elution Buffer<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\nMultiple Temperatures<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u83ccDNA\u7d14\u5316\u8a66\u5291\u76d2 | \u00a0\u8ca8\u865fK1457 Bacterial DNA Purification Kit, K […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1440],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25627"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25627"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25627\/revisions"}],"predecessor-version":[{"id":40979,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25627\/revisions\/40979"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25627"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25627"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25627"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}