{"id":25619,"date":"2020-07-07T11:46:31","date_gmt":"2020-07-07T03:46:31","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25619"},"modified":"2022-07-19T16:52:17","modified_gmt":"2022-07-19T08:52:17","slug":"anti-rhodopsin-rho-1d4-mouse-igg1-fc-silent-kappa%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9fab00337-1-4","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25619","title":{"rendered":"Anti-Rhodopsin [Rho 1D4], Mouse IgG1, Fc Silent\u2122, Kappa\u6297\u9ad4 |\u8ca8\u865fAb00337-1.4"},"content":{"rendered":"

Anti-Rhodopsin [Rho 1D4], Mouse IgG1, Fc Silent\u2122, Kappa\u6297\u9ad4 |\u8ca8\u865fAb00337-1.4<\/a> \u00a0\u00a0<\/strong><\/span><\/p>\n

\"Binding<\/p>\n

\u6297\u9ad4\u4ecb\u7d39<\/strong>:<\/strong>\u00a0\u00a0Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: ‘TETSQVAPA’. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.Application Notes: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.<\/p>\n

Antibody first published in: Molday et al. 1983 Monoclonal antibodies to rhodopsin: characterization, corss-reactivity, and applicatoin as sturctural probes. Biochemistry, 1983; 22(3):653-660 PMID:6188482<\/p>\n

Note on publication: Describes the generation of two monoclonal antibodies from hybridoma.<\/p>\n

UniProt Accession Number of Target Protein: P08100<\/p>\n

Alternative Name(s) of Target: Rho-tag; Visual Purple<\/p>\n

Immunogen: Bleached bovine rod outer segment (ROS).<\/p>\n

Purified antibody.<\/p>\n

Purification:<\/strong>\u00a0Protein A affinity purified<\/p>\n

Supplied in:<\/strong>\u00a0PBS with 0.02% Proclin 300.<\/p>\n

Storage Recommendation:<\/strong>\u00a0Store at 4\u2070C for up to 3 months. For longer storage, aliquot and store at -20\u2070C.<\/p>\n

Concentration:<\/strong>\u00a01 mg\/ml.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\nMouse target<\/td>\n\u6297\u9ad4clone<\/td>\nOriginal Antibody format<\/td>\n<\/tr>\n
Ab01052<\/a><\/td>\n4-1BB (CD137)<\/td>\nLOB12.3<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n<\/thead>\n
Ab01054<\/a><\/td>\n4-1BBL (CD137L)<\/td>\nAT113-2<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00881<\/a><\/td>\n4-1BBL (CD137L)<\/td>\nTKS-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01027<\/a><\/td>\nBTLA<\/td>\nHMBT-6B2
\n<\/a><\/td>\n		Hamster IgG<\/td>\n<\/tr>\n
Ab00670<\/a><\/td>\nCD27<\/td>\nLG.3A10<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00895<\/a><\/td>\nCD27<\/td>\n\n

RM27-3E5<\/p>\n

<\/a><\/td>\n

Rat IgG2a<\/td>\n<\/tr>\n
Ab00286<\/a><\/td>\nCD28<\/td>\nE18
\n<\/a><\/td>\n		Mouse IgG2b<\/td>\n<\/tr>\n
Ab00285<\/a><\/td>\nCD28<\/td>\nD665
\n<\/a><\/td>\n		Mouse IgG1<\/td>\n<\/tr>\n
Ab01109<\/a><\/td>\nCD40<\/td>\n3\/23
\n<\/a><\/td>\n		Rat IgG2a<\/td>\n<\/tr>\n
Ab01087<\/a><\/td>\nCD40L (CD154)<\/td>\nMR1
\n<\/a><\/td>\n		Hamster IgG<\/td>\n<\/tr>\n
Ab00816<\/a><\/td>\nCD70<\/td>\nTAN 1-7
\n<\/a><\/td>\n		Rat IgG2a<\/td>\n<\/tr>\n
Ab01056<\/a><\/td>\nCD70<\/td>\nTAN1-6
\n<\/a><\/td>\n		Rat IgG2a<\/td>\n<\/tr>\n
Ab01028<\/td>\nCD80 (B7-1)<\/td>\nRM80<\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01029<\/a><\/td>\nCD86 (B7-2)<\/td>\nPO.3<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab01089<\/a><\/td>\nCD153<\/td>\nRM153<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab01297<\/a><\/td>\nCD96<\/td>\n6A6<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00894<\/a><\/td>\nCTLA-4<\/td>\n9H10<\/a><\/td>\nHamster IgG2<\/td>\n<\/tr>\n
Ab01018<\/a><\/td>\nCTLA-4<\/td>\n9D9<\/a><\/td>\nMouse IgG2b<\/td>\n<\/tr>\n
Ab01088<\/a><\/td>\nGAL-9<\/td>\nRG9-35<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01060<\/a><\/td>\nGITR<\/td>\nDTA-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00374<\/a><\/td>\nGITR<\/td>\nYGITR 860.103.5<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00631<\/a><\/td>\nGITR<\/td>\nYGITR765<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00630<\/a><\/td>\nGITRL<\/td>\nYGL386<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01026<\/a><\/td>\nHVEM<\/td>\nHMHV-1B18<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00814<\/a><\/td>\nICOS<\/td>\n7E.17G9<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00882<\/a><\/td>\nICOSL<\/td>\nHK5.3<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01295<\/a><\/td>\nLAG3<\/td>\nC9B7W<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01295<\/a><\/td>\nLAG3<\/td>\nC9B7W<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00110<\/a><\/td>\nOX40 (CD134)<\/td>\nOX86<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00564<\/a><\/td>\nOX40L<\/td>\nOX-89<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00813<\/a><\/td>\nPD-1<\/td>\nRMP1-14<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01017<\/a><\/td>\nPD-1H (VISTA)<\/td>\nmam82<\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01016<\/a><\/td>\nPD-1H (VISTA)<\/td>\nMH5A<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00373<\/a><\/td>\nPD-L1<\/td>\nYDC 127.1.1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00883<\/a><\/td>\nPD-L2<\/td>\nTY25<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00875<\/a><\/td>\nPDPN (MAP-Tag)<\/td>\nPMab-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00973<\/a><\/td>\nTIGIT<\/td>\n4D4<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab01258<\/a><\/td>\nTIGIT<\/td>\n1B4<\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01032<\/a><\/td>\nTIM-1<\/td>\n3B3<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01110<\/a><\/td>\nTIM-2<\/td>\nRMT2-14<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01057<\/a><\/td>\nTIM-3<\/td>\n2C12<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01058<\/a><\/td>\nTIM-4<\/td>\n5G3<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01059<\/a><\/td>\nTIM-4<\/td>\n3A1<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
 <\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Anti-Rhodopsin [Rho 1D4], Mouse IgG1, Fc Silent\u2122, Kappa […]<\/p>\n","protected":false},"author":3,"featured_media":3519,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1442,1305,1443,1427],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25619"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25619"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25619\/revisions"}],"predecessor-version":[{"id":25620,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25619\/revisions\/25620"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/3519"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25619"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25619"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25619"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}