{"id":25614,"date":"2020-07-02T15:45:43","date_gmt":"2020-07-02T07:45:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25614"},"modified":"2022-07-19T16:52:34","modified_gmt":"2022-07-19T08:52:34","slug":"goat-anti-mouse-igg3-human-ads-pe%e6%8a%97%e9%ab%94-southern-biotech%e8%b2%a8%e8%99%9f1100-09s","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25614","title":{"rendered":"Goat Anti-Mouse IgG3, Human ads-PE\u6297\u9ad4 | Southern Biotech\u8ca8\u865f1100-09S"},"content":{"rendered":"

Goat Anti-Mouse IgG3, Human ads-PE\u6297\u9ad4 | \u8ca8\u865f1100-09S\u00a0<\/a><\/span> \u00a0\u898f\u683c0.25 mg\u00a0 \u00a0(PE\u6a19\u8a8c)<\/p>\n

\u7d30\u80de\u514d\u75ab\u5c08\u5bb6\u6975\u529b\u63a8\u85a6SouthernBiotech\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4<\/p>\n

\"\"<\/p>\n

FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG3, Human ads-PE (SB Cat. No. 1100-09S).<\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\u3002\u7269\u7a2e\u7a2e\u985e\u9f4a\u5168\uff0c\u5305\u62ec\u6709Bovine\u3001Canine\u3001Chicken\u3001Cynomolgus\u3001Donkey\u3001Equine\u3001Feline\u3001Goat\u3001Guinea Pig\u3001Hamster\u3001Human\u3001Monkey\u3001Mouse\u3001Porcine\u3001Rabbit\u3001Rat\u3001Rhesus\u3001Sheep\u3001Turkey\u3002\u5177\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001PE\u3001APC\u3001SPRD\u3001PE\/CY5.5\u3001APC\/CY5.5\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u5404\u7a2e\u87a2\u5149\u6a19\u8a8c\u3001\u53e6\u5177\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001\u6216\u672a\u6a19\u8a8c\u53caLow Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Specificity<\/strong><\/td>\nReacts with the heavy chain of mouse IgG3<\/sub><\/td>\n<\/tr>\n
Source<\/strong><\/td>\nPooled antisera from goats hyperimmunized with mouse IgG3<\/sub><\/td>\n<\/tr>\n
Cross Adsorption<\/strong><\/td>\nMouse IgG1<\/sub>, IgG2a<\/sub>, IgG2b<\/sub>, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species<\/td>\n<\/tr>\n
Purification<\/strong><\/td>\nAffinity chromatography on mouse IgG3<\/sub>\u00a0covalently linked to agarose<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nGoat IgG<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nGoat IgG-PE<\/a><\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nPE (R-phycoerythrin)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nPhosphate buffered saline containing 0.1% sodium azide and a stabilizer<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.5 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n0.5 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C; Avoid exposure to light; Do not freeze
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Quality tested applications for relevant formats include –<\/p>\n

ELISA\u00a01-9<\/sup>
\nFLISA\u00a02<\/sup>
\nFlow Cytometry\u00a010-16<\/sup><\/p>\n

Other referenced applications for relevant formats include –<\/p>\n

ELISpot\u00a01,3,4,10<\/sup>
\nImmunohistochemistry-Frozen Sections\u00a017-21<\/sup>
\nImmunohistochemistry-Paraffin Sections\u00a017,22<\/sup>
\nImmunocytochemistry\u00a023<\/sup>
\nWestern Blot\u00a01,24<\/sup><\/td>\n<\/tr>\n

Recommended Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2794577<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n
Gene ID<\/strong><\/td>\n380795<\/a>\u00a0(Mouse)<\/td>\n<\/tr>\n
Gene Symbol<\/strong><\/td>\nIghg3<\/a>\u00a0(Mouse)<\/td>\n<\/tr>\n
Gene Aliases<\/strong><\/td>\nIgG3<\/td>\n<\/tr>\n
Uniprot ID<\/strong><\/td>\nP03987<\/a>\u00a0(Mouse)<\/td>\n<\/tr>\n
Uniprot Name<\/strong><\/td>\nIGHG3_MOUSE<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Isotype control \u5206\u578b\u5c0d\u7167\u7d44<\/a> |\u5206\u578b\u5c0d\u7167\u7d44\u5957\u7d44<\/a> | \u6297\u9ad4\u5206\u578bELISA \u5957\u7d44<\/a> | anti-rabbit \u4e8c\u7d1a\u6297\u9ad4<\/a> | anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a> |<\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

References<\/p>\n

    \n
  1. McLay J, Leonard E, Petersen S, Shapiro D, Greenspan NS, Schreiber JR. \u03b33 gene-disrupted mice selectively deficient in the dominant IgG subclass made to bacterial polysaccharides. II. Increased susceptibility to fatal pneumococcal sepsis due to absence of anti-polysaccharide IgG3 is corrected by induction of anti-polysaccharide IgG1. J Immunol. 2002;168:3437-43. (ELISA, ELISPOT, WB)<\/li>\n
  2. Szmacinski H, Smith DS, Hanson MA, Kostov Y, Lakowicz JR, Rao G. A novel method for monitoring monoclonal antibody production during cell culture. Biotechnol Bioeng. 2008;100:448-57. (ELISA, FLISA)<\/li>\n
  3. Hovden A, Brokstad KA, Major D, Wood J, Haaheim LR, Cox RJ. A pilot study of the immune response to whole inactivated avian influenza H7N1 virus vaccine in mice. Influenza Other Respir Viruses. 2009;3:21-8. (ELISA, ELISPOT)<\/li>\n
  4. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Ig\u03b1 serine\/threonines fine-tune Ig \u03b1 tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA, ELISPOT)<\/li>\n
  5. F\u00f6rster M, Raposo B, Ekman D, Klaczkowska D, Popovic M, Nandakumar KS, et al. Genetic control of antibody production during collagen-induced arthritis development in heterogeneous stock mice. Arthritis Rheum. 2012;64:3594-603. (ELISA)<\/li>\n
  6. Li X, Wu J, Ptacek T, Redden DT, Brown EE, Alarc\u00f3n GS, et al. Allelic-dependent expression of an activating Fc receptor on B cells enhances humoral immune responses. Sci Transl Med. 2013;5(216):216ra175. (ELISA)<\/li>\n
  7. Brachs S, Turqueti-Neves A, Stein M, Reimer D, Brachvogel B, B\u00f6sl M, et al. Swiprosin-1\/EFhd2 limits germinal center responses and humoral type 2 immunity. Eur J Immunol. 2014;44:3206-19. (ELISA)<\/li>\n
  8. Pucella JN, Yen W, Kim MV, van der Veeken J, Socci ND, Naito Y, et al. miR-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function. J Immunol. 2015;194:2635-42. (ELISA)<\/li>\n
  9. Patel PS, Kearney JF. Neonatal exposure to pneumococcal phosphorylcholine modulates the development of house dust mite allergy during adult life. J Immunol. 2015;194:5838-50. (ELISA)<\/li>\n
  10. Conway KL, Kuballa P, Khor B, Zhang M, Shi HN, Virgin HW, et al. ATG5 regulates plasma cell differentiation. Autophagy. 2013;9:528-37. (ELISPOT, FC)<\/li>\n
  11. Cook AJ, Oganesian L, Harumal P, Basten A, Brink R, Jolly CJ. Reduced switching in SCID B cells is associated with altered somatic mutation of recombined S regions. J Immunol. 2003;171:6556-64. (FC)<\/li>\n
  12. Martomo SA, Saribasak H, Yokoi M, Hanaoka F, Gearhart PJ. Reevaluation of the role of DNA polymerase \u03b8 in somatic hypermutation of immunoglobulin genes. DNA Repair. 2008;7:1603-8. (FC)<\/li>\n
  13. Johansson S, Salmon-Divon M, Johansson MH, Pickman Y, Brodin P, K\u00e4rre K, et al. Probing natural killer cell education by Ly49 receptor expression analysis and computational modelling in single MHC class I mice. PLoS One. 2009;4(6):e6046. (FC)<\/li>\n
  14. Brodin P, Lakshmikanth T, Johansson S, K\u00e4rre K, H\u00f6glund P. The strength of inhibitory input during education quantitatively tunes the functional responsiveness of individual natural killer cells. Blood. 2009;113:2434-41. (FC)<\/li>\n
  15. Pauklin S, Petersen-Mahrt SK. Progesterone inhibits activation-induced deaminase by binding to the promoter. J Immunol. 2009;183:1238-44. (FC)<\/li>\n
  16. Bai L, Deng S, Reboulet R, Mathew R, Teyton L, Savage PB, et al. Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci USA. 2013;110:16097-102. (FC)<\/li>\n
  17. van der Loos C. User Protocol: Practical Guide to Multiple Staining. BioTechniques’ Protocol Guide 2010. 2009 Nov. doi: doi10.2144\/000113281. (IHC-FS, IHC-PS)<\/li>\n
  18. Halstensen TS, Farstad IN, Scott H, Fausa O, Brandtzaeg P. Intraepithelial TcR\u03b1\/\u03b2+ lymphocytes express CD45RO more often than the TcR\u03b3\/\u03b4+ counterparts in coeliac disease. Immunology. 1990;71:460-6. (IHC-FS)<\/li>\n
  19. Ladinser B, Pittschieler K. Expression of the VLA family of integrins in coeliac intestinal mucosa. Acta Paediatr. 1998;87:1125-30. (IHC-FS)<\/li>\n
  20. Richards HB, Satoh M, Jennette JC, Croker BP, Yoshida H, Reeves WH. Interferon-\u03b3 is required for lupus nephritis in mice treated with the hydrocarbon oil pristane. Kidney Int. 2001;60:2173-80. (IHC-FS)<\/li>\n
  21. Jacob N, Guo S, Mathian A, Koss MN, Gindea S, Putterman C, et al. B Cell and BAFF dependence of IFN-\u03b1-exaggerated disease in systemic lupus erythematosus-prone NZM 2328 mice. J Immunol. 2011;186:4984-93. (IHC-FS)<\/li>\n
  22. Chalan P, Bijzet J, Huitema MG, Kroesen B, Brouwer E, Boots AM. Expression of lectin-like transcript 1, the ligand for CD161, in rheumatoid arthritis. PLoS One. 2015;10(7):e0132436. (IHC-PS)<\/li>\n
  23. H\u00e4ggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)<\/li>\n
  24. Esser-von Bieren J, Volpe B, Kulagin M, Sutherland DB, Guiet R, Seitz A, et al. Antibody-mediated trapping of helminth larvae requires CD11b and Fc\u03b3 receptor I. J Immunol. 2015;194:1154-63. (WB)<\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    <\/td>\n <\/td>\n<\/tr>\n
    \u00a0Cat. No.<\/strong><\/td>\nProduct Description<\/strong><\/td>\nClone<\/strong><\/td>\nSize<\/strong><\/td>\n<\/tr>\n
    1100-30<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-Alexa Fluor<\/strong>\u00ae<\/sup><\/strong>488<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mg<\/td>\n<\/tr>\n
    1100-32<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-Alexa Fluor<\/strong>\u00ae<\/sup><\/strong>555<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mg<\/td>\n<\/tr>\n
    1100-31<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-Alexa Fluor<\/strong>\u00ae<\/sup><\/strong>647<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mg<\/td>\n<\/tr>\n
    1100-04<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-AP<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mL<\/td>\n<\/tr>\n
    1100-19<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-APC\/CY7<\/strong><\/td>\nPolyclonal<\/td>\n0.25 mg<\/td>\n<\/tr>\n
    1100-08<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-BIOT<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mg<\/td>\n<\/tr>\n
    1100-15<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-CY5<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mg<\/td>\n<\/tr>\n
    1100-02<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-FITC<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mg<\/td>\n<\/tr>\n
    1100-05<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-HRP<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mL<\/td>\n<\/tr>\n
    1100-17<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-PE\/CY7<\/strong><\/td>\nPolyclonal<\/td>\n0.25 mg<\/td>\n<\/tr>\n
    1100-09<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-PE<\/strong><\/td>\nPolyclonal<\/td>\n0.5 mg<\/td>\n<\/tr>\n
    1100-09S<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-PE<\/strong><\/td>\nPolyclonal<\/td>\n0.25 mg<\/td>\n<\/tr>\n
    1100-13<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-SPRD<\/strong><\/td>\nPolyclonal<\/td>\n0.25 mg<\/td>\n<\/tr>\n
    1100-03<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-TRITC<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mg<\/td>\n<\/tr>\n
    1100-07<\/strong><\/a><\/td>\nGoat Anti-Mouse IgG<\/strong>3<\/sub><\/strong>, Human ads-TXRD<\/strong><\/td>\nPolyclonal<\/td>\n1.0 mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Goat Anti-Mouse IgG3, Human ads-PE\u6297\u9ad4 | \u8ca8\u865f1100-09S\u00a0 \u00a0\u898f\u683c0 […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198,3],"tags":[1445,1446],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25614"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25614"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25614\/revisions"}],"predecessor-version":[{"id":25615,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25614\/revisions\/25615"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25614"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25614"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}