{"id":25506,"date":"2020-06-20T11:59:44","date_gmt":"2020-06-20T03:59:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25506"},"modified":"2025-03-17T15:12:00","modified_gmt":"2025-03-17T07:12:00","slug":"%e7%99%bd%e4%bb%8b%e7%b4%a0-1%ce%b2-elisa%e8%a9%a6%e5%8a%91%e7%9b%92-elikine-mouse-il-1%ce%b2-elisa-kit%e8%b2%a8%e8%99%9fket7005","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=25506","title":{"rendered":"\u767d\u4ecb\u7d20-1\u03b2 ELISA\u8a66\u5291\u76d2(Mouse) | EliKine\u2122 Mouse IL-1\u03b2 ELISA Kit\u8ca8\u865fKTE7005"},"content":{"rendered":"

\"\"\u00a0\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2 | \u8ca8\u865fKTE7005<\/a>\u00a0<\/span><\/strong>15.6 pg\/ml-1000 pg\/ml<\/u><\/strong>, \u6700\u4f4e\u9748\u654f\u5ea68 pg\/mL<\/u><\/strong><\/span><\/p>\n

\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2\u63a1\u7528\u5169\u9ede\u593e\u5fc3ELISA(Sandwich ELISA)\u5b9a\u91cf\u6a23\u54c1\u4e2d\u7684\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09\u3002\u5c0d\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09\u7279\u7570\u7684\u6297\u9ad4\u5df2\u9810\u5148\u5305\u88ab\u5728\u5fae\u5b54\u677f\u4e0a\u3002\u5c07\u6a19\u6e96\u54c1\u548c\u6837\u54c1\u5438\u53d6\u5230\u5b54\u4e2d\uff0c\u4e26\u4e14\u5b58\u5728\u7684\u4efb\u4f55\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09\u90fd\u88ab\u56fa\u5b9a\u7684\u6297\u9ad4\u7d50\u5408\u3002\u9664\u53bb\u6240\u6709\u672a\u7d50\u5408\u7684\u7269\u8cea\u5f8c\uff0c\u5c07HRP-\u7db4\u5408\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09\u6aa2\u6e2c\u6297\u9ad4\u6dfb\u52a0\u5230\u5b54\u4e2d\u3002\u6d17\u6ecc\u4ee5\u9664\u53bb\u4efb\u4f55\u672a\u7d50\u5408\u7684HRP\u8a66\u5291\u5f8c\uff0c\u5c07\u8272\u539f\u6eb6\u6db2\u6dfb\u52a0\u5230\u5b54\u4e2d\uff0c\u4e26\u4e14\u6839\u64da\u5728\u521d\u59cb\u6b65\u9a5f\u4e2d\u7d50\u5408\u7684\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09\u7684\u91cf\u6210\u6bd4\u4f8b\u5730\u986f\u8272\u3002\u505c\u6b62\u986f\u8272\u4e26\u6e2c\u91cf\u984f\u8272\u7684\u5f37\u5ea6\u3002\u5075\u6e2c\u7bc4\u570d: 15.6 pg\/ml-1000 pg\/ml<\/u><\/strong>, \u6700\u4f4e\u9748\u654f\u5ea68 pg\/mL<\/u><\/strong>\u3002<\/u><\/strong>\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: Cell culture supernatants, Other biological fluids, Plasma, Serum\u3002<\/p>\n

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\"\u7269\u7a2emouse-2\"<\/a>\u00a0\"\u7269\u7a2eHUMAN\"<\/a>\u00a0\u00a0\"\u7269\u7a2eRAT\"\u00a0\"\u7269\u7a2eporcine\"\u00a0\"\u7269\u7a2erabbit\"<\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)\u529f\u80fd<\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)\u5177\u6709\u591a\u7a2e\u751f\u7269\u5b78\u529f\u80fd\u3002\u5b83\u662f\u6025\u6027\u671f\u53cd\u61c9(acute phase response)\u7684\u6709\u6548\u8a98\u5c0e\u5291\u3002\u5728B\u7d30\u80de(B-cells)\u6700\u7d42\u5206\u5316\u70baIg\u5206\u6ccc\u7d30\u80de(Ig-secreting cells)\u7684\u6700\u7d42\u5206\u5316\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\uff0c\u53c3\u8207\u6dcb\u5df4\u7d30\u80de(lymphocyte)\u548c\u55ae\u6838\u7d30\u80de(monocyte)\u7684\u5206\u5316\u3002\u5b83\u8a98\u5c0e\u9aa8\u9ad3\u7624(myeloma)\u548c\u6f3f\u7d30\u80de\u7624(plasmacytoma growth)\u751f\u9577\uff0c\u4e26\u8a98\u5c0e\u795e\u7d93\u7d30\u80de\u5206\u5316(differentiation)\u3002\u4f5c\u7528\u65bcB\u7d30\u80de(B-cells)\uff0cT\u7d30\u80de(T-cells)\uff0c\u809d\u7d30\u80de(hepatocytes)\uff0c\u9020\u8840\u7956\u7d30\u80de(hematopoeitic progenitor cells)\u548cCNS\u4e2d\u6a1e\u795e\u7d93\u7cfb\u7d71\u7d30\u80de\u3002\u4e5f\u53ef\u4ee5\u4f5c\u70ba\u808c\u8089\u56e0\u5b50(myokine)\u3002\u5b83\u5728\u808c\u8089\u6536\u7e2e\u5f8c\u6392\u5165\u8840\u6db2\uff0c\u4e26\u8d77\u5230\u589e\u52a0\u8102\u80aa\u5206\u89e3\u548c\u6539\u5584\u80f0\u5cf6\u7d20\u62b5\u6297(insulin resistance)\u7684\u4f5c\u7528\u3002<\/p>\n

\u767d\u4ecb\u7d20-1\uff08Interleukin-1, IL-1\uff09\u662f\u6709\u53f2\u4ee5\u4f86\u6700\u65e9\u7684\u7d30\u80de\u56e0\u5b50(cytokines)\u4e4b\u4e00\u3002\u5b83\u7684\u6700\u521d\u767c\u73fe\u662f\u53ef\u80fd\u8a98\u767c\u767c\u71d2(fever)\uff0c\u63a7\u5236\u6dcb\u5df4\u7d30\u80de(lymphocytes)\uff0c\u589e\u52a0\u9aa8\u9ad3\u7d30\u80de\u6578\u91cf(bone marrow cells)\u4e26\u5f15\u8d77\u9aa8\u95dc\u7bc0\u9000\u5316\u7684\u56e0\u7d20\u3002\u6b64\u6642\uff0cIL-1\u4ee5\u5176\u4ed6\u5e7e\u7a2e\u540d\u7a31\u70ba\u4eba\u6240\u77e5\uff0c\u5305\u62ec\u5167\u6e90\u6027\u71b1\u539f(endogenous pyrogen)\uff0c\u6dcb\u5df4\u7d30\u80de\u6d3b\u5316\u56e0\u5b50(lymphocyte activating factor)\uff0c\u9020\u8840\u7d20-1(haemopoetin-1)\u548c\u55ae\u6838\u7d30\u80de\u56e0\u5b50(mononuclear cell factor)\u7b49\u3002\u5927\u7d04\u57281984\u5e74\u81f31985\u5e74\u9593\uff0c\u79d1\u5b78\u5bb6\u5011\u8b49\u5be6IL-1\u5be6\u969b\u4e0a\u7531\u5169\u7a2e\u4e0d\u540c\u7684\u86cb\u767d\u8cea\u7d44\u6210\uff0c\u73fe\u5728\u7a31\u70baIL-1\u03b1\u548cIL-1\u03b2\uff0c\u5b83\u5011\u5c6c\u65bc\u7a31\u70ba\u767d\u7d30\u80de\u4ecb\u7d201\u8d85\u5bb6\u65cf\u7684\u7d30\u80de\u56e0\u5b50\u5bb6\u65cf\u3002 IL-1\u03b2\u7531\u5de8\u566c\u7d30\u80de(macrophages)\uff0c\u55ae\u6838\u7d30\u80de(monocytes)\u548c\u6a39\u7a81\u72c0\u7d30\u80de(dendritic cells)\u7522\u751f\u3002<\/p>\n

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Fig.1. Mouse IL-1\u03b2 Standard Curve.<\/p>\n

\"ELISA<\/a><\/p>\n

ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a>\u00a0<\/a> (\u6a19\u914d405\u3001450\u3001492\u3001630 nm\u6ffe\u7247\/\u6700\u9ad8\u53ef\u64f4\u5145\u81f37\u6ffe\u7247\uff09\uff08KTE7005<\/a>\u00a0 absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.\u03bb= 570 nm\uff09<\/span><\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nEliKine\u2122 Mouse IL-1\u03b2 ELISA Kit<\/a><\/td>\n<\/tr>\n
Reactivity<\/strong><\/td>\nMouse<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nELISA<\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nEliKine\u2122 Mouse IL-1\u03b2 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1\u03b2 in samples. An antibody specific for IL-1\u03b2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1\u03b2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1\u03b2 is added to the wells. After washing, proprietary EliKine\u2122 Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1\u03b2 bound in the initial step. The color development is stopped and the intensity of the color is measured.<\/td>\n<\/tr>\n
Detection method<\/strong><\/td>\nColorimetric<\/td>\n<\/tr>\n
SampleType<\/strong><\/td>\nCell culture supernatants, Other biological fluids, Plasma, Serum<\/td>\n<\/tr>\n
Assay type<\/strong><\/td>\nSandwich ELISA (quantitative)<\/td>\n<\/tr>\n
Assay duration<\/strong><\/td>\nMultiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nIL1B; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Mouse IL-1\u03b2 microplate
\n\u2022 Mouse IL-1\u03b2 standard
\n\u2022 Mouse IL-1\u03b2 detect antibody
\n\u2022 EliKine\u2122 Streptavidin-HRP
\n\u2022 Standard diluent
\n\u2022 Assay buffer
\n\u2022 HRP substrate
\n\u2022 Stop solution
\n\u2022 Wash buffer
\n\u2022 Plate covers.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\nEliKine\u2122 Mouse IL-1\u03b2 ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-1\u03b2. No significant cross-reactivity or interference between Mouse IL-1\u03b2 and analogues was observed.<\/td>\n<\/tr>\n
Calibration range<\/strong><\/td>\n15.6 pg\/ml-1000 pg\/ml<\/td>\n<\/tr>\n
Limit of detection<\/strong><\/td>\n8 pg\/mL<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix components from different kit lots or use reagents beyond the kit expiration date.
\n\u2022 Allow all reagents to warm to room temperature for at least 30 minutes before opening.
\n\u2022 Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
\n\u2022 Unused wells must be kept desiccated at 4 \u00b0C in the sealed bag provided.
\n\u2022 Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
\n\u2022 It is recommended that all samples and standards be assayed in duplicate or triplicate.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nThe unopened kit should be stored at 2 – 8\u00b0C. After opening, please store refer to protocols.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nInterleukin-1 (IL-1) is one of the first cytokines ever described. Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints. At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others. It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1\u03b1 and IL-1\u03b2.These belong to a family of cytokines known as the interleukin-1 superfamily.Both IL-1\u03b1 and IL-1\u03b2 are produced by macrophages, monocytes and dendritic cells.<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n16176<\/a><\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nIL1B; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta<\/td>\n<\/tr>\n
Accession<\/strong><\/td>\nP10749<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805<\/p>\n